##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936449_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 831656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.315850543974914 31.0 31.0 34.0 28.0 34.0 2 31.332590638437047 31.0 31.0 34.0 28.0 34.0 3 31.38982584145368 31.0 31.0 34.0 28.0 34.0 4 35.003429302500074 37.0 35.0 37.0 32.0 37.0 5 34.971633704320055 37.0 35.0 37.0 32.0 37.0 6 35.12420399780679 37.0 35.0 37.0 32.0 37.0 7 34.97806064045711 37.0 35.0 37.0 32.0 37.0 8 34.929278451667514 37.0 35.0 37.0 32.0 37.0 9 36.37651384707139 39.0 37.0 39.0 32.0 39.0 10-11 36.28368941004454 39.0 36.0 39.0 32.0 39.0 12-13 36.190140514828244 39.0 35.0 39.0 32.0 39.0 14-15 37.210929158209645 39.5 36.5 41.0 31.5 41.0 16-17 37.09657839298941 39.0 36.0 41.0 31.0 41.0 18-19 37.078459122521814 39.0 36.0 41.0 31.0 41.0 20-21 36.99555044393355 39.0 36.0 41.0 31.0 41.0 22-23 36.88013373317814 39.0 36.0 41.0 30.5 41.0 24-25 36.982869720172765 39.0 36.0 41.0 31.0 41.0 26-27 36.95351082659177 39.0 36.0 41.0 30.5 41.0 28-29 36.882697293111576 39.0 36.0 41.0 30.0 41.0 30-31 36.703894398645595 39.0 36.0 41.0 30.0 41.0 32-33 36.52389990573026 39.0 35.0 41.0 30.0 41.0 34-35 36.274914147195474 39.0 35.0 40.0 30.0 41.0 36-37 36.05678790269053 38.5 35.0 40.0 29.5 41.0 38-39 35.854247429225545 38.0 35.0 40.0 28.5 41.0 40-41 35.69416080687208 38.0 35.0 40.0 28.0 41.0 42-43 35.444836567042145 38.0 35.0 40.0 27.0 41.0 44-45 35.14192105870697 38.0 34.0 40.0 26.5 41.0 46-47 34.8132418932828 38.0 34.0 40.0 24.5 41.0 48-49 34.677410491837975 38.0 34.0 40.0 24.0 41.0 50-51 33.727743802726124 37.5 33.0 39.5 23.5 40.5 52-53 33.87051316890638 37.5 33.0 39.5 23.0 40.5 54-55 34.28116973844955 38.0 34.0 40.0 23.0 41.0 56-57 34.169022408303434 38.0 33.5 40.0 23.0 41.0 58-59 33.89482911203671 38.0 33.0 40.0 22.0 41.0 60-61 33.53667922795002 37.0 33.0 40.0 21.5 41.0 62-63 33.22324855469088 37.0 32.5 40.0 20.0 41.0 64-65 32.884946420154485 36.0 32.0 39.0 19.5 41.0 66-67 32.461393893629094 36.0 31.5 39.0 18.0 41.0 68-69 32.03645437536674 35.0 31.0 39.0 15.5 40.0 70-71 31.567901271679638 35.0 31.0 38.0 12.0 40.0 72-73 31.181492107313602 35.0 30.5 37.0 9.5 39.5 74-75 30.775172066335116 35.0 30.0 37.0 7.5 39.0 76-77 30.356170700385736 34.5 30.0 36.0 6.5 39.0 78-79 29.889807805150205 34.0 29.0 36.0 2.0 38.0 80-81 29.396324321594506 34.0 29.0 35.0 2.0 37.0 82-83 28.92019356560886 34.0 29.0 35.0 2.0 37.0 84-85 28.597266177361796 34.0 28.5 35.0 2.0 36.0 86-87 28.302207884028974 34.0 28.0 35.0 2.0 36.0 88-89 27.87979344825264 34.0 27.0 35.0 2.0 35.5 90-91 27.433565079792608 33.0 26.0 35.0 2.0 35.0 92-93 27.078667742431968 33.0 25.5 35.0 2.0 35.0 94-95 26.61265294785344 33.0 25.0 35.0 2.0 35.0 96-97 26.011786123108593 33.0 24.0 35.0 2.0 35.0 98-99 25.313296002193212 32.5 19.0 35.0 2.0 35.0 100-101 22.930375058918592 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4530347606387721 1101 2 0.4843680973188498 1101 3 0.4857172884856098 1101 4 0.5882852624479042 1101 5 0.5564233870902768 1101 6 0.4882271988438305 1101 7 0.43074569466843116 1101 8 0.628427930660898 1101 9 0.7308601845422373 1101 10-11 0.6215085915167435 1101 12-13 0.775331289458066 1101 14-15 0.9975618721325574 1101 16-17 1.049821274053933 1101 18-19 0.9241113281705111 1101 20-21 0.8918719768581909 1101 22-23 0.8963397640371227 1101 24-25 1.0601083285823094 1101 26-27 0.7375578894888832 1101 28-29 0.9091964868006954 1101 30-31 0.785365545297239 1101 32-33 0.81167215752982 1101 34-35 0.9013790338275527 1101 36-37 0.8863806405125629 1101 38-39 0.7623650367946553 1101 40-41 0.7104808931315105 1101 42-43 0.7480755696240919 1101 44-45 0.804683123497469 1101 46-47 0.965022321377873 1101 48-49 0.8635715338876722 1101 50-51 0.7966057588206539 1101 52-53 0.9529311047283642 1101 54-55 1.0566196546940816 1101 56-57 1.1045863430850176 1101 58-59 0.9585672643311156 1101 60-61 1.062519978081447 1101 62-63 1.090267628647645 1101 64-65 0.9572753850914495 1101 66-67 1.042057414639281 1101 68-69 0.9188814757517463 1101 70-71 1.0418107269110841 1101 72-73 0.8763232889720562 1101 74-75 0.9217175444208578 1101 76-77 0.850099672156535 1101 78-79 0.8179831912352924 1101 80-81 0.8379153603274503 1101 82-83 1.0912128599376878 1101 84-85 0.9941008149293005 1101 86-87 0.7429595255087023 1101 88-89 0.9702660820155238 1101 90-91 0.9349832363488382 1101 92-93 0.8449163171810596 1101 94-95 1.087558374986802 1101 96-97 0.5780916117808133 1101 98-99 0.5500834957521263 1101 100-101 0.8896915316806222 1102 1 0.29769141676802846 1102 2 0.43526084992393166 1102 3 0.25178683537707514 1102 4 0.3752876626352375 1102 5 0.4041358914808626 1102 6 0.3138009021572543 1102 7 0.2919812057327107 1102 8 0.42223134946431884 1102 9 0.6814821843080736 1102 10-11 0.5952726413938052 1102 12-13 0.44402860241908115 1102 14-15 0.5309947254284282 1102 16-17 0.5912793878579379 1102 18-19 0.6673615330645575 1102 20-21 0.7265334626326947 1102 22-23 0.7063927437818833 1102 24-25 0.38306451052822155 1102 26-27 0.4383138021454869 1102 28-29 0.5232163615226284 1102 30-31 0.3800683025856131 1102 32-33 0.4728505909685339 1102 34-35 0.14830855027761913 1102 36-37 0.4526967920170719 1102 38-39 0.3889163773870834 1102 40-41 0.2750525182339558 1102 42-43 0.44884538580486577 1102 44-45 0.13386442545274235 1102 46-47 0.2964442860190175 1102 48-49 0.4244433580300466 1102 50-51 0.35106732655277284 1102 52-53 0.3910070033350834 1102 54-55 0.49815460376327536 1102 56-57 0.6320077515851921 1102 58-59 0.54820255293901 1102 60-61 0.5404331322788423 1102 62-63 0.5655522850110657 1102 64-65 0.340970210053392 1102 66-67 0.496262864820757 1102 68-69 0.041230293220429814 1102 70-71 -0.05777875324346482 1102 72-73 -0.18827614317874364 1102 74-75 -0.26676521520916197 1102 76-77 0.004950538565623219 1102 78-79 0.139800015963079 1102 80-81 0.28695967598135397 1102 82-83 0.156760658790283 1102 84-85 0.17453489758941032 1102 86-87 0.11597205331575111 1102 88-89 0.15503513716128836 1102 90-91 0.23070168266659508 1102 92-93 -0.06730853939311388 1102 94-95 0.10001227382769073 1102 96-97 -0.0037935597447713576 1102 98-99 0.2512345612011373 1102 100-101 0.0766273413047891 1103 1 0.30119735073684595 1103 2 0.41968260360109966 1103 3 0.32183923105916534 1103 4 0.31160244705541373 1103 5 0.4374821133102955 1103 6 0.3610185651835778 1103 7 0.3194376478765122 1103 8 0.3283373402477281 1103 9 0.49480126138654157 1103 10-11 0.41818825593833964 1103 12-13 0.31600835432868024 1103 14-15 0.4508861464245726 1103 16-17 0.5654717526076354 1103 18-19 0.505623006456382 1103 20-21 0.5412693536749202 1103 22-23 0.47586042259003847 1103 24-25 0.46061051456836566 1103 26-27 0.5232938381280619 1103 28-29 0.1563822759219775 1103 30-31 0.07273409073029313 1103 32-33 0.35696933347215776 1103 34-35 0.25537092007750317 1103 36-37 0.19484493607362907 1103 38-39 0.26905494187165147 1103 40-41 0.09857810058355909 1103 42-43 -0.03928509838173255 1103 44-45 0.22754932539592687 1103 46-47 0.45215257463716085 1103 48-49 0.38585480617094703 1103 50-51 0.6161654106383665 1103 52-53 0.4046247515832988 1103 54-55 0.6191034075004112 1103 56-57 0.6538478342819971 1103 58-59 0.40234212746081965 1103 60-61 0.405477558135793 1103 62-63 0.6320928285373597 1103 64-65 0.7350275612307158 1103 66-67 0.5061205916056863 1103 68-69 0.6284623966229859 1103 70-71 0.44515469477117975 1103 72-73 0.5841889000634737 1103 74-75 0.35716990567967244 1103 76-77 0.34737802104778837 1103 78-79 0.4891695317764757 1103 80-81 0.36792441936876585 1103 82-83 0.19447805352546155 1103 84-85 0.41437534326834324 1103 86-87 0.423228539648683 1103 88-89 0.4835710566108169 1103 90-91 0.5259635821678934 1103 92-93 0.5240943076493707 1103 94-95 0.4407487330158695 1103 96-97 0.35525298087766544 1103 98-99 0.35666311684787644 1103 100-101 0.7005151166977548 1104 1 0.1893891653100077 1104 2 0.28874315020875585 1104 3 0.21397319099893863 1104 4 0.39868553324421185 1104 5 0.3339049814035562 1104 6 0.25714485984069313 1104 7 0.4573260450111576 1104 8 0.4541620425336248 1104 9 0.3398895490171512 1104 10-11 0.5324123742026998 1104 12-13 0.3729322033956137 1104 14-15 0.4534686166531614 1104 16-17 0.2689758818223993 1104 18-19 0.6026253334171869 1104 20-21 0.6144157696990149 1104 22-23 0.09025107824546552 1104 24-25 0.08637133949878972 1104 26-27 -0.044984210875000485 1104 28-29 0.41267875810475374 1104 30-31 0.3116285562137051 1104 32-33 0.2681204946712299 1104 34-35 0.03117509035627819 1104 36-37 0.17903529059295664 1104 38-39 0.2583623288315451 1104 40-41 0.23530529864898142 1104 42-43 0.39949301906134593 1104 44-45 0.6395354548844523 1104 46-47 0.45370388184161214 1104 48-49 0.31244603672256943 1104 50-51 0.2559016884136085 1104 52-53 0.359675625334269 1104 54-55 0.6007044477887789 1104 56-57 0.47711744234879205 1104 58-59 0.3234399054413828 1104 60-61 0.5488616891756308 1104 62-63 0.6730382133984278 1104 64-65 0.45425008256118815 1104 66-67 0.532609162121723 1104 68-69 0.5656869255274941 1104 70-71 0.7332552559793761 1104 72-73 0.5122926551394436 1104 74-75 0.2154511485505033 1104 76-77 0.534025372418867 1104 78-79 0.6398288373383636 1104 80-81 0.5282132362688898 1104 82-83 0.6345656567558322 1104 84-85 0.45425990776166003 1104 86-87 0.7080987317370173 1104 88-89 0.6176131244120704 1104 90-91 0.7656086065781658 1104 92-93 0.676311764418088 1104 94-95 0.49071907567225637 1104 96-97 0.6401208916318737 1104 98-99 1.053603847787329 1104 100-101 1.3780896022569138 1105 1 0.2040016232335411 1105 2 0.01626746410194002 1105 3 0.11494465027111289 1105 4 0.19926007574829896 1105 5 0.3322986745934742 1105 6 0.19648457454306367 1105 7 0.25492151884425596 1105 8 0.2642856130302107 1105 9 0.22874188940068763 1105 10-11 0.39596127062058173 1105 12-13 0.13816032089174968 1105 14-15 0.07881098477702153 1105 16-17 0.27903585791968055 1105 18-19 0.08304593361674506 1105 20-21 0.2524435758018839 1105 22-23 0.03497877360636181 1105 24-25 -0.020831274450316073 1105 26-27 0.09521896226623738 1105 28-29 -0.12054767085043494 1105 30-31 -0.2311776014784641 1105 32-33 -0.1340855731116264 1105 34-35 -0.08231350102777668 1105 36-37 0.017382713375106107 1105 38-39 -0.2419013686345437 1105 40-41 -0.5671776240618911 1105 42-43 -0.33538412313792065 1105 44-45 -0.744783043410564 1105 46-47 -0.32045486293652203 1105 48-49 -0.1202360022454414 1105 50-51 -0.05850682885007785 1105 52-53 -0.22669379233839493 1105 54-55 -0.3789711111292391 1105 56-57 0.1965192164132361 1105 58-59 0.04013014589400399 1105 60-61 5.513265779057974E-4 1105 62-63 -0.1567579555872456 1105 64-65 0.06692839358166225 1105 66-67 0.25202135251019797 1105 68-69 0.2390436133557472 1105 70-71 0.12648574181865513 1105 72-73 0.2671203472109731 1105 74-75 -0.07107286929978684 1105 76-77 -0.2890632321226505 1105 78-79 -0.11390623716576442 1105 80-81 -0.33419753969242905 1105 82-83 -0.14357431670995524 1105 84-85 0.026742720827019895 1105 86-87 0.06338857965868527 1105 88-89 -0.1225697643086967 1105 90-91 -0.11148863803001419 1105 92-93 -0.05502547229561472 1105 94-95 0.1428313965388952 1105 96-97 0.04078150586605389 1105 98-99 0.08173724252215209 1105 100-101 0.47305468016005037 1106 1 0.12177824956574668 1106 2 0.21345929154766097 1106 3 0.14762877149801312 1106 4 0.18596087938957595 1106 5 -0.05617196862434071 1106 6 0.12186074165168037 1106 7 0.3925361977433397 1106 8 0.27134668367139625 1106 9 0.3592243789614784 1106 10-11 0.3184672903103021 1106 12-13 0.47890523844485955 1106 14-15 0.5681228605834505 1106 16-17 0.5964741624499368 1106 18-19 0.4033442257520292 1106 20-21 0.09897450735521574 1106 22-23 0.31393170318869323 1106 24-25 0.3717845728273588 1106 26-27 0.20842468833733818 1106 28-29 0.29898152337472794 1106 30-31 0.32594087211109724 1106 32-33 0.13333596772574197 1106 34-35 0.005157416505014112 1106 36-37 -0.1459870936900316 1106 38-39 -0.17408969094447713 1106 40-41 -0.21527215097015784 1106 42-43 -0.22757833813990658 1106 44-45 -0.029923299641069434 1106 46-47 -0.06869467518315986 1106 48-49 0.005776169211578974 1106 50-51 -0.2982682764203517 1106 52-53 0.008480499268578967 1106 54-55 0.04555671152105134 1106 56-57 -0.02124623412370852 1106 58-59 0.1036950213854837 1106 60-61 0.28823661618340424 1106 62-63 0.3952602914384684 1106 64-65 0.2854609100659644 1106 66-67 0.33423964627105107 1106 68-69 -0.13112412517128647 1106 70-71 0.24171327085032246 1106 72-73 0.2894820975437078 1106 74-75 -0.09117914095289237 1106 76-77 0.10887127771713523 1106 78-79 0.30716724876797485 1106 80-81 0.4813561193645377 1106 82-83 0.4846272946365282 1106 84-85 0.06325709188374162 1106 86-87 0.4799866675817128 1106 88-89 0.5264747051966232 1106 90-91 0.3740082276674066 1106 92-93 0.4036952039415951 1106 94-95 0.41998603906584364 1106 96-97 0.6893332243985704 1106 98-99 0.8275336195325238 1106 100-101 1.4934251069738345 1107 1 0.1748329281952934 1107 2 0.15271779960171372 1107 3 0.13323557996277202 1107 4 0.2102460209125212 1107 5 0.4421922882833442 1107 6 0.24264461804273907 1107 7 0.15429720742302777 1107 8 0.3659974163023634 1107 9 0.24627556036892884 1107 10-11 0.20808495431418805 1107 12-13 0.2766660729265311 1107 14-15 0.2626213057009892 1107 16-17 0.2232442521041662 1107 18-19 0.28501480629763876 1107 20-21 0.09673652105435337 1107 22-23 0.3164021637910466 1107 24-25 0.28017622127230624 1107 26-27 0.3367218646168695 1107 28-29 0.2881706260576138 1107 30-31 0.40504783387412147 1107 32-33 0.18218168228099074 1107 34-35 0.2883717611801728 1107 36-37 0.13589726213715636 1107 38-39 -0.0407901056921105 1107 40-41 0.18252843998624968 1107 42-43 0.36804019511570374 1107 44-45 0.558304066964169 1107 46-47 0.24043044652892576 1107 48-49 0.23923343268582187 1107 50-51 0.26097308244247586 1107 52-53 0.41737752662979943 1107 54-55 0.4701322774612038 1107 56-57 0.47415987152744066 1107 58-59 0.6604019049832388 1107 60-61 0.7851453818122494 1107 62-63 0.43469669287105006 1107 64-65 0.853790666439636 1107 66-67 0.7835038145689737 1107 68-69 0.8630589635025032 1107 70-71 0.8453833325402407 1107 72-73 0.8626744413900447 1107 74-75 1.0072172917457998 1107 76-77 0.7006127123867998 1107 78-79 0.46628916974692913 1107 80-81 0.69757743489955 1107 82-83 0.903843317169688 1107 84-85 0.6006736622476474 1107 86-87 0.5874479134418209 1107 88-89 0.2296622984811485 1107 90-91 0.2399093014291438 1107 92-93 0.16199605264125694 1107 94-95 0.20072823854227195 1107 96-97 0.4735819112556854 1107 98-99 0.7256761835556134 1107 100-101 0.811723698314335 1108 1 -0.09612337736056986 1108 2 0.006348215436307925 1108 3 0.06175998684590667 1108 4 0.1062083026885432 1108 5 0.3642807657631053 1108 6 0.12643196022455783 1108 7 0.09796259793683504 1108 8 0.30903392457161516 1108 9 0.20137483917706334 1108 10-11 0.1632605448566764 1108 12-13 0.22820352451914516 1108 14-15 0.28214607667383973 1108 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-1.6236377558142934 2316 64-65 -2.0437519438533016 2316 66-67 -2.214823157411846 2316 68-69 -2.813797405311334 2316 70-71 -2.8347371716296337 2316 72-73 -2.2239099507584896 2316 74-75 -1.775445984250748 2316 76-77 -1.8386411401928946 2316 78-79 -2.6625397567921425 2316 80-81 -2.2168016063284774 2316 82-83 -2.216529318091741 2316 84-85 -2.09349681584607 2316 86-87 -2.499408778439495 2316 88-89 -2.8940183170085554 2316 90-91 -2.8139610469760576 2316 92-93 -3.391530459660693 2316 94-95 -3.9548183685569036 2316 96-97 -4.501776116740416 2316 98-99 -5.547363142987152 2316 100-101 -5.757481971058407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2113.0 3 817.0 4 793.0 5 870.0 6 1000.0 7 1133.0 8 1332.0 9 1697.0 10 2197.0 11 2824.0 12 3660.0 13 4304.0 14 4993.0 15 6175.0 16 7455.0 17 9360.0 18 7847.0 19 5700.0 20 5781.0 21 6369.0 22 6916.0 23 7864.0 24 9108.0 25 10071.0 26 12185.0 27 14150.0 28 16595.0 29 20203.0 30 24545.0 31 29632.0 32 36635.0 33 46911.0 34 61498.0 35 82906.0 36 112222.0 37 141739.0 38 106525.0 39 15525.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.73556779099296 18.361857514228006 14.056344229341871 21.84623046543716 2 15.615403952624318 20.825550248557548 39.20497668382253 24.354069114995607 3 20.743277212884852 24.811829314936027 29.437737092657674 25.00715637952145 4 12.710314557425018 16.35845397143187 37.5623243368113 33.368907134331806 5 13.543057911898831 38.1358216318329 33.569529172532455 14.751591283735818 6 31.265054587543407 35.94969331353728 17.391011403586873 15.394240695332446 7 28.573456813147548 33.34352037789925 20.298368107652195 17.784654701301015 8 26.732660164366155 32.83749983457513 19.617345189384412 20.812494811674302 9 26.420471976756076 17.639274048497025 20.83808057171388 35.10217340303302 10-11 25.43313339630247 26.27738104548818 26.55472599401994 21.734759564189414 12-13 27.39731136578696 23.940599074432228 26.869498312911205 21.792591246869605 14-15 23.487747630911894 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21.787408501807864 40-41 22.336323069467774 26.88934769721376 28.843418231578493 21.93091100173997 42-43 21.874844055594668 27.22664561554977 28.07119542246429 22.827314906391273 44-45 22.49924544773508 26.69221121691544 28.014932318766167 22.793611016583316 46-47 23.151164258788302 27.320551437211794 27.59909576802602 21.929188535973886 48-49 22.92687120739795 27.27348700241873 27.495579493660383 22.304062296522936 50-51 22.047075091685205 27.6720976372272 28.15968255876871 22.121144712318884 52-53 22.829296448723998 27.963780772664826 26.968774143896873 22.238148634714303 54-55 22.021724033226405 27.38310479906923 27.54184116142105 23.053330006283314 56-57 22.775121458695025 27.465450646486794 27.100608511219182 22.658819383599003 58-59 23.550514179471545 27.053500882120552 26.848334193205446 22.547650745202453 60-61 22.556876091747366 27.360774963996764 28.255098328668193 21.827250615587673 62-63 22.33164054937113 27.537702824419863 27.995331716309774 22.13532490989924 64-65 21.97779008255221 28.204286510460157 27.450067966844443 22.367855440143185 66-67 22.200683170096845 27.651012038152185 26.96267096539114 23.18563382635983 68-69 22.085779264278404 27.5267804633408 27.531410744416903 22.85602952796389 70-71 22.651313186700868 27.621473227008558 26.97897915231186 22.748234433978716 72-73 22.495546415578495 27.114107356185148 27.838284532789988 22.552061695446373 74-75 21.920068204104872 28.685285313090148 27.027980878320246 22.36666560448473 76-77 22.34201950480405 27.702172945802616 26.95655310910425 22.999254440289086 78-79 22.421550277507382 27.933037477776264 27.109383783900785 22.536028460815576 80-81 22.823102153463186 27.91162473280882 26.830594028746912 22.434679084981074 82-83 22.530421408205452 27.732094390243024 27.332127580513465 22.40535662103806 84-85 22.264429830469336 27.968350106798546 27.137534396828755 22.629685665903363 86-87 22.425424150706526 28.106981336046168 26.778342550471802 22.689251962775504 88-89 22.1087504847685 28.059633289541324 26.42215294333394 23.40946328235623 90-91 22.2899693965284 27.557975240649107 26.91415996777316 23.237895395049332 92-93 22.716270913900548 27.994905436065686 27.003413814855413 22.28540983517835 94-95 22.318189088398952 27.69491916775802 27.04365530337195 22.94323644047108 96-97 21.677736369113546 27.853920815249612 27.5101046360467 22.958238179590136 98-99 22.00887879637102 28.22072865787287 26.404015303956246 23.366377241799864 100-101 21.958281706744938 27.517111958522296 26.880751602930385 23.64385473180238 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 246.0 1 174.5 2 162.5 3 229.0 4 218.5 5 210.0 6 279.5 7 463.0 8 735.5 9 900.0 10 838.0 11 798.5 12 889.5 13 979.5 14 1051.0 15 1081.0 16 1123.5 17 1238.5 18 1364.0 19 1461.5 20 1605.0 21 1833.5 22 2146.0 23 2544.5 24 3015.0 25 3709.0 26 4705.0 27 5836.0 28 6885.5 29 8048.5 30 9464.0 31 11121.0 32 13204.0 33 14604.0 34 15473.0 35 16613.0 36 18205.5 37 19941.0 38 20914.0 39 21384.5 40 21974.5 41 23194.5 42 24334.5 43 25496.0 44 26831.0 45 28273.0 46 29427.5 47 34085.0 48 45687.5 49 45041.5 50 34015.5 51 29282.5 52 27107.5 53 26837.0 54 26509.5 55 24641.5 56 22497.5 57 20306.5 58 18538.5 59 16431.0 60 13769.5 61 11484.0 62 9578.5 63 7492.5 64 5483.5 65 4301.0 66 3228.5 67 2478.0 68 1852.0 69 1289.0 70 1015.5 71 788.0 72 645.5 73 549.0 74 433.5 75 351.5 76 272.0 77 179.0 78 109.0 79 72.5 80 55.0 81 40.0 82 29.5 83 21.5 84 15.5 85 12.0 86 6.0 87 4.5 88 4.0 89 2.5 90 1.5 91 2.0 92 1.5 93 0.0 94 0.0 95 1.0 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044850274632780865 2 0.031142683994343816 3 0.02765566532316246 4 0.062525852034976 5 0.06805698509960849 6 0.06180439989611089 7 0.06432948238213877 8 0.05567205671575747 9 0.05663399290091096 10-11 0.06745577498388756 12-13 0.05897871235222256 14-15 0.05158382792885521 16-17 0.0617442788845388 18-19 0.06811710611118059 20-21 0.015030252893023078 22-23 0.028016391392595017 24-25 0.009018151735813846 26-27 0.015030252893023078 28-29 0.009198514770530124 30-31 0.014008195696297509 32-33 0.0067936743076464304 34-35 0.012324807372278923 36-37 0.01460940581201843 38-39 0.018096424483199786 40-41 0.003967986763758093 42-43 0.003547139682753446 44-45 0.00444895485633483 46-47 0.001322662254586031 48-49 0.0024649614744557844 50-51 7.214521388651077E-4 52-53 0.0064329482382138765 54-55 0.010641419048260338 56-57 0.024108525640409013 58-59 0.016473157170753292 60-61 0.016052310089748646 62-63 0.010461056013544062 64-65 6.012101157209231E-5 66-67 0.011002145117692892 68-69 0.02080187000394394 70-71 0.006372827226641785 72-73 0.002705445520744154 74-75 0.004148349798474369 76-77 0.007935973527516186 78-79 0.0067936743076464304 80-81 0.010641419048260338 82-83 0.010461056013544062 84-85 0.022124532258529967 86-87 0.006372827226641785 88-89 0.00883778870109757 90-91 0.0061323431803534155 92-93 0.021823927200669505 94-95 0.01430880075415797 96-97 0.012865896476427753 98-99 0.028497359485171753 100-101 0.044008580470771566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 831656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.89582402007258 #Duplication Level Percentage of deduplicated Percentage of total 1 85.06472584858021 54.352807531366 2 9.198124484198758 11.754434867141693 3 2.44646488549335 4.689566693843102 4 1.006095359434346 2.571411681353146 5 0.5292857337214425 1.690957404910014 6 0.323194194493906 1.2390455625415036 7 0.2225329907694205 0.995325017180433 8 0.1786655807240569 0.9132787603510729 9 0.13879644398640392 0.7981661843610411 >10 0.793647506428238 9.515807835618418 >50 0.06168448791778483 2.6593838538534484 >100 0.03168952491160724 3.854043729750062 >500 0.001886281237228424 0.7444859763534502 >1k 0.0030180499795654783 3.4563095910454735 >5k 1.886281237228424E-4 0.7649753103311232 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6347 0.7631761208961397 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3553 0.42721990823128797 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3210 0.3859768942928326 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2702 0.32489394653558684 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2468 0.29675731311984765 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2423 0.2913464220783593 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1899 0.22833960195080658 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1520 0.1827678751791606 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1480 0.17795819425339324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.17411044951277932 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1355 0.16292794136037017 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1222 0.1469357522821936 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1130 0.13587348615292863 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1127 0.13551276008349605 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1096 0.13178525736602634 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1022 0.12288734765335668 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1022 0.12288734765335668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 6.0121011572092306E-5 0.0 22-23 0.0 0.0 0.0 1.8036303471627693E-4 6.0121011572092306E-5 24-25 0.0 0.0 0.0 5.410891041488308E-4 1.2024202314418461E-4 26-27 0.0 0.0 0.0 9.619361851534769E-4 1.2024202314418461E-4 28-29 0.0 0.0 0.0 0.0019238723703069536 1.2024202314418461E-4 30-31 0.0 0.0 0.0 0.004268591821618554 1.2024202314418461E-4 32-33 0.0 0.0 0.0 0.007214521388651077 2.4048404628836923E-4 34-35 0.0 0.0 0.0 0.010340813990399877 2.4048404628836923E-4 36-37 1.2024202314418461E-4 0.0 0.0 0.014008195696297507 2.4048404628836923E-4 38-39 1.2024202314418461E-4 6.0121011572092306E-5 0.0 0.021884048212241602 2.4048404628836923E-4 40-41 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.03643333301268793 2.4048404628836923E-4 42-43 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.05609290379676212 2.4048404628836923E-4 44-45 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.07803707302057582 3.0060505786046155E-4 46-47 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.12481122002366363 3.6072606943255385E-4 48-49 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.16599411295054686 3.6072606943255385E-4 50-51 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.19599449772502092 3.6072606943255385E-4 52-53 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.2313456525294112 3.6072606943255385E-4 54-55 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.26838019565782006 3.6072606943255385E-4 56-57 1.2024202314418461E-4 1.2024202314418461E-4 0.0 0.3477399309329819 3.6072606943255385E-4 58-59 1.2024202314418461E-4 1.8036303471627693E-4 0.0 0.4330516463537809 3.6072606943255385E-4 60-61 1.2024202314418461E-4 2.4048404628836923E-4 0.0 0.5281029656492588 3.6072606943255385E-4 62-63 1.2024202314418461E-4 2.4048404628836923E-4 0.0 0.6212304125744298 3.6072606943255385E-4 64-65 1.2024202314418461E-4 2.4048404628836923E-4 0.0 0.7257207306867263 3.6072606943255385E-4 66-67 1.2024202314418461E-4 2.4048404628836923E-4 0.0 0.8813740296468733 3.6072606943255385E-4 68-69 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.0752041709552989 3.6072606943255385E-4 70-71 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.2078311224833345 3.6072606943255385E-4 72-73 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.318994872880133 3.6072606943255385E-4 74-75 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.4542070279057686 3.6072606943255385E-4 76-77 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.6090186327039064 3.6072606943255385E-4 78-79 1.2024202314418461E-4 2.4048404628836923E-4 0.0 1.7786801273603507 3.6072606943255385E-4 80-81 1.2024202314418461E-4 2.4048404628836923E-4 0.0 2.0415893109651106 4.2084708100464615E-4 82-83 1.8036303471627693E-4 2.4048404628836923E-4 0.0 2.3517536096655345 4.8096809257673845E-4 84-85 2.4048404628836923E-4 2.4048404628836923E-4 0.0 2.754504266186981 4.8096809257673845E-4 86-87 2.4048404628836923E-4 2.4048404628836923E-4 0.0 3.145290841405581 4.8096809257673845E-4 88-89 2.4048404628836923E-4 2.4048404628836923E-4 0.0 3.60557730600152 4.8096809257673845E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 4560 0.0 24.806776 1 TACATGG 4635 0.0 24.097744 2 GAGTACT 3185 0.0 23.435766 12-13 ACATGGG 4740 0.0 22.46094 3 GTACTAT 155 3.8864386E-5 21.46469 1 GTACTTT 3490 0.0 21.25015 14-15 AGTACTT 3350 0.0 19.443066 12-13 CTAGACT 225 1.6558515E-6 19.012726 4 CTATACT 350 6.366463E-11 19.012726 4 ACTATAC 225 1.6568065E-6 19.011581 3 GTGTAAG 400 1.8189894E-12 19.011581 1 CATGGGA 2775 0.0 18.84144 4 CATATAC 305 4.5220077E-9 18.699917 3 ACTTTTT 4170 0.0 18.691881 16-17 TACCTGG 560 0.0 18.672089 2 ACTAGAC 235 2.511044E-6 18.202578 3 TCGCGCT 105 1.9615956E-5 18.089935 60-61 CATGGGG 1925 0.0 18.025051 4 GTGTAGC 400 2.1827873E-11 17.823357 1 GTATAAA 410 3.274181E-11 17.388643 1 >>END_MODULE