FastQCFastQC Report
Thu 26 May 2016
SRR936449_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936449_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831656
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT64380.7741181450022606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47750.5741556605134815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42950.5164394894042729No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40520.48722067778023603No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT28830.34665775272468424No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26800.3222486220264148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24590.29567513491154995No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT21700.26092519022288063No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20430.24565445328356916No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14980.18012255066998856No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC13080.15727656627259348No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12760.15342882153197956No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.1524668853468261No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG11680.14044268303240762No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA11300.13587348615292863No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9680.11639427840357071No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA9270.11146435545465914No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG8580.10316765585771041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTACT45900.023.1804112-13
TACCTGG6700.021.9791972
GTACATG42750.021.5648921
GTACTTT50000.021.32647514-15
GGCTCGT1150.003720868820.6536646
CTATACC1150.003722165220.6524244
GTATAGG2108.566112E-720.365961
TACATGG44000.020.1889572
ACATGGG42250.020.0120753
AGTACTT48050.019.86956812-13
GCCACGT2651.8513674E-819.7184058
GTGTATC1951.0282325E-519.4956191
ACTTTTT57000.019.08241716-17
CAATTGG1250.00601470919.0082281
GTACCTG7600.018.758121
GCTATCG650.007565300418.26890220-21
ATGGGAG10450.018.1820375
CATGGGA25200.017.9069614
AGTACAT34900.017.83082
AGAGTAC82000.017.49411210-11
Produced by FastQC (version 0.11.5)