##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936449_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 831656 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.825032224862202 31.0 31.0 34.0 30.0 34.0 2 31.951629038929557 31.0 31.0 34.0 30.0 34.0 3 32.05389127235299 33.0 31.0 34.0 30.0 34.0 4 35.81605736025472 37.0 35.0 37.0 35.0 37.0 5 35.58379546350895 37.0 35.0 37.0 33.0 37.0 6 35.699215781525055 37.0 35.0 37.0 33.0 37.0 7 35.64177977432977 37.0 35.0 37.0 33.0 37.0 8 35.624343478553634 37.0 35.0 37.0 33.0 37.0 9 37.244807949440634 39.0 37.0 39.0 33.0 39.0 10-11 37.10417348038131 39.0 37.0 39.0 33.0 39.0 12-13 37.006200881133545 39.0 37.0 39.0 33.0 39.0 14-15 38.05266841097762 40.0 37.0 41.0 33.0 41.0 16-17 37.99054837577076 40.0 37.0 41.0 33.0 41.0 18-19 38.175082606269896 40.0 37.5 41.0 33.0 41.0 20-21 38.21993408332291 40.0 38.0 41.0 33.0 41.0 22-23 38.124090369094915 40.0 37.0 41.0 33.0 41.0 24-25 37.98147250786383 40.0 37.0 41.0 33.0 41.0 26-27 37.735029868118545 40.0 37.0 41.0 32.5 41.0 28-29 37.63752020065989 39.5 37.0 41.0 32.0 41.0 30-31 37.582436127437305 39.0 37.0 41.0 32.0 41.0 32-33 37.40324184518599 39.0 36.0 41.0 32.0 41.0 34-35 37.185064497821216 39.0 36.0 41.0 31.5 41.0 36-37 36.911414094288986 39.0 35.5 41.0 31.0 41.0 38-39 36.709226531161924 39.0 35.0 41.0 31.0 41.0 40-41 36.48018411458584 39.0 35.0 40.5 30.0 41.0 42-43 36.195593490577835 39.0 35.0 40.0 30.0 41.0 44-45 35.87640863530113 39.0 35.0 40.0 29.5 41.0 46-47 35.42611308040824 38.0 35.0 40.0 27.5 41.0 48-49 34.96099529132238 38.0 34.0 40.0 26.0 41.0 50-51 34.609210418730825 38.0 34.0 40.0 24.0 41.0 52-53 34.333458184634026 38.0 34.0 40.0 24.0 41.0 54-55 34.06700065892629 38.0 33.0 40.0 23.0 41.0 56-57 33.72541110747713 37.5 33.0 40.0 22.5 41.0 58-59 33.36625660128707 37.0 32.5 40.0 21.5 41.0 60-61 32.967691569591274 36.5 32.0 39.0 20.0 41.0 62-63 32.57869299325682 36.0 31.5 39.0 18.5 40.0 64-65 32.17173566955569 35.5 31.0 39.0 17.5 40.0 66-67 31.68609797801014 35.0 31.0 38.0 14.0 40.0 68-69 31.39266535683023 35.0 30.5 38.0 12.5 40.0 70-71 30.931618361437902 35.0 30.0 37.0 8.0 39.5 72-73 30.466772920534453 34.0 29.5 37.0 7.5 39.0 74-75 29.96548813451716 34.0 29.0 36.0 6.5 39.0 76-77 28.542210962224765 32.5 27.0 34.5 2.0 36.5 78-79 29.32165101917139 34.0 29.0 35.0 2.0 37.0 80-81 29.109655915426572 34.0 29.0 35.0 2.0 37.0 82-83 28.74611197418163 34.0 29.0 35.0 2.0 36.5 84-85 28.323004944351993 33.5 27.5 35.0 2.0 36.0 86-87 27.970817260982905 33.0 27.0 35.0 2.0 35.5 88-89 27.560050068778438 33.0 26.5 35.0 2.0 35.0 90-91 27.053389261906368 33.0 25.5 35.0 2.0 35.0 92-93 26.574235020248757 32.0 25.0 34.0 2.0 35.0 94-95 25.95882672643497 32.0 24.0 34.0 2.0 35.0 96-97 25.185606789345595 32.0 20.5 34.0 2.0 35.0 98-99 24.23853131583251 31.0 9.5 34.0 2.0 35.0 100-101 21.119362452744884 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3805468091640627 1101 2 0.4036250756855786 1101 3 0.33393721491401607 1101 4 0.2933647389704248 1101 5 0.3198351008670457 1101 6 0.32549942520832076 1101 7 0.32652951927004636 1101 8 0.25595014206147937 1101 9 0.4360742689291186 1101 10-11 0.38232177793042155 1101 12-13 0.3401735019405976 1101 14-15 0.5560583051244663 1101 16-17 0.6461671904273132 1101 18-19 0.5489185308901554 1101 20-21 0.4018203913634437 1101 22-23 0.46539086608083124 1101 24-25 0.38179376214220184 1101 26-27 0.34606477649553113 1101 28-29 0.42989606997102925 1101 30-31 0.49029360071411077 1101 32-33 0.5823383201210888 1101 34-35 0.49739631134544027 1101 36-37 0.5991716905974371 1101 38-39 0.5619676325972094 1101 40-41 0.5888996024031314 1101 42-43 0.7784494698975593 1101 44-45 0.6612648529655942 1101 46-47 0.6390462802643029 1101 48-49 0.5669127711486013 1101 50-51 0.8618395159078105 1101 52-53 0.7116298588415475 1101 54-55 0.7894569848488473 1101 56-57 0.5478832323830787 1101 58-59 0.4447255128726155 1101 60-61 0.6139734096548608 1101 62-63 0.7445614016139004 1101 64-65 0.6574897347605031 1101 66-67 0.6461736522331663 1101 68-69 0.4468469653218037 1101 70-71 0.6238831286275897 1101 72-73 0.7211069189648391 1101 74-75 0.8342856744368667 1101 76-77 0.6129749744065975 1101 78-79 0.2777890107138994 1101 80-81 0.3802403436738615 1101 82-83 0.650381471383298 1101 84-85 0.5808414806625244 1101 86-87 0.4901730069245325 1101 88-89 0.12073330081300782 1101 90-91 0.15438838594540982 1101 92-93 0.1415179734218519 1101 94-95 0.5347448959646961 1101 96-97 0.6962293321258208 1101 98-99 0.6237161837630758 1101 100-101 1.2549769119146852 1102 1 0.206212714795722 1102 2 0.23210388378767632 1102 3 0.2275518384943922 1102 4 0.22504428469106585 1102 5 0.15013746592968857 1102 6 0.1988090493741126 1102 7 0.1569109148911636 1102 8 0.23345570347115085 1102 9 0.29255623825355315 1102 10-11 0.2641432859505599 1102 12-13 0.30094068376942573 1102 14-15 0.3451842856888021 1102 16-17 0.3077854142886949 1102 18-19 0.16785220376218746 1102 20-21 0.035996717320593064 1102 22-23 0.11237819192088949 1102 24-25 0.24200041241734738 1102 26-27 0.05984170542657097 1102 28-29 0.06360918904373847 1102 30-31 0.3354756637044005 1102 32-33 0.23300203045946688 1102 34-35 0.20102220409804517 1102 36-37 0.22797393857776171 1102 38-39 0.22677781290396837 1102 40-41 0.22983084581725421 1102 42-43 0.13755554646939316 1102 44-45 0.1386167438448851 1102 46-47 0.0860562908017144 1102 48-49 -0.07043355832102094 1102 50-51 -0.28755751020154463 1102 52-53 -0.1666345422824449 1102 54-55 -0.09129907416063787 1102 56-57 -0.1408329601002407 1102 58-59 -0.32695593543554224 1102 60-61 -0.31008846834536996 1102 62-63 -0.28178358140916515 1102 64-65 -0.22759544261919729 1102 66-67 -0.12587880064120682 1102 68-69 -0.3085132091308367 1102 70-71 -0.22700596515533888 1102 72-73 -0.40135385846169314 1102 74-75 -0.4044363721618929 1102 76-77 -0.4222748499699378 1102 78-79 -0.4677406403808213 1102 80-81 -0.35091379518575394 1102 82-83 -0.4340729250574 1102 84-85 -0.26641586625235547 1102 86-87 -0.2555300723401892 1102 88-89 -0.3660254218190033 1102 90-91 -0.2959504209858608 1102 92-93 -0.14142240241235626 1102 94-95 -0.07179561451416916 1102 96-97 0.1879625893556529 1102 98-99 0.09361432215436949 1102 100-101 0.3926386318538029 1103 1 0.16861644562079547 1103 2 0.19550344820911292 1103 3 0.14087722830564076 1103 4 0.12707225009728518 1103 5 -0.1352714413362719 1103 6 0.20207983404679908 1103 7 0.18840277146587425 1103 8 0.1401677166016313 1103 9 0.11558114198437863 1103 10-11 0.11653344440015445 1103 12-13 0.09753101897553051 1103 14-15 0.1767621532476653 1103 16-17 0.33186178257351884 1103 18-19 0.048566016021517555 1103 20-21 0.1610487973660426 1103 22-23 0.11857137732585699 1103 24-25 0.1810178355598282 1103 26-27 0.2950387415985247 1103 28-29 0.15802439326064643 1103 30-31 0.20396881438932724 1103 32-33 0.2366669663852221 1103 34-35 0.21927586548306266 1103 36-37 0.28073260280351064 1103 38-39 0.3801034394426992 1103 40-41 0.4064820199855532 1103 42-43 0.2676436090916994 1103 44-45 0.5234748651756078 1103 46-47 0.4105457485379631 1103 48-49 0.4760171076722912 1103 50-51 0.5288271877341941 1103 52-53 0.469653018477608 1103 54-55 0.6590599625163378 1103 56-57 0.5931626841141409 1103 58-59 0.5947097580181548 1103 60-61 0.48615624474092556 1103 62-63 0.4339485377752297 1103 64-65 0.33133470699257117 1103 66-67 0.4688153474555037 1103 68-69 0.5172810115623037 1103 70-71 0.2030217319597405 1103 72-73 0.3872792770726825 1103 74-75 0.3643203351166875 1103 76-77 0.11200605085702975 1103 78-79 0.15374119473059622 1103 80-81 0.3756399261702228 1103 82-83 0.5277397761609564 1103 84-85 0.5438786227319241 1103 86-87 0.39505701180853947 1103 88-89 0.270315797169701 1103 90-91 0.3653852743616568 1103 92-93 0.20404308272115657 1103 94-95 0.2633891907380246 1103 96-97 0.5537306122077439 1103 98-99 0.8276273263838974 1103 100-101 1.1877214075624032 1104 1 0.14314306879002459 1104 2 0.20467441138837827 1104 3 0.1778485291223575 1104 4 0.10340112722462891 1104 5 -0.05001342244682405 1104 6 0.12456010050662059 1104 7 0.16102219930727557 1104 8 -0.017198711285395518 1104 9 0.03575269831051742 1104 10-11 0.0990561436746944 1104 12-13 0.13481258123314177 1104 14-15 0.032723279314197384 1104 16-17 0.025480891028145436 1104 18-19 0.09808921718956043 1104 20-21 0.12452920070591489 1104 22-23 0.12479257548253742 1104 24-25 0.05130802126042511 1104 26-27 0.20627887482844187 1104 28-29 0.1592563136877061 1104 30-31 0.08812723023090996 1104 32-33 0.24027603696969635 1104 34-35 0.14274623095278827 1104 36-37 0.3979658007776834 1104 38-39 0.33262431364993006 1104 40-41 0.404855428754999 1104 42-43 0.34168887076072707 1104 44-45 0.4605328566890492 1104 46-47 0.5030219085963736 1104 48-49 0.5182582903158917 1104 50-51 0.45574236773145316 1104 52-53 0.6521985310933829 1104 54-55 0.7079261394566103 1104 56-57 0.5358624149167142 1104 58-59 0.271542809986947 1104 60-61 0.3923477855539943 1104 62-63 0.5255760415394377 1104 64-65 0.7214907502466659 1104 66-67 0.4396576160141592 1104 68-69 0.6757036971488333 1104 70-71 0.11588617508607868 1104 72-73 0.2981019261578659 1104 74-75 0.38855494751802055 1104 76-77 0.17709023208621133 1104 78-79 0.36363990349548203 1104 80-81 0.5044554574929379 1104 82-83 0.4905608739389393 1104 84-85 0.7043796037056005 1104 86-87 0.4406722876218332 1104 88-89 0.5883223674119762 1104 90-91 0.7938357922245025 1104 92-93 0.8752321140666872 1104 94-95 0.7162842037416297 1104 96-97 1.0175714663392377 1104 98-99 1.1819162345373861 1104 100-101 1.6704334575692492 1105 1 -0.024288923578648536 1105 2 0.08196792147028376 1105 3 -0.035895536314086485 1105 4 -0.12421199953654849 1105 5 -0.15592660512722034 1105 6 -0.10260347963876626 1105 7 -0.0281656563553625 1105 8 -0.031439470909532474 1105 9 -0.13976126504594788 1105 10-11 -0.23543669099083075 1105 12-13 -0.2940954590725511 1105 14-15 -0.05957193027320784 1105 16-17 0.09639577321404147 1105 18-19 -0.2314361273714738 1105 20-21 -0.1368328158944152 1105 22-23 -0.09586080214758397 1105 24-25 -0.08520229090036224 1105 26-27 -0.12230845234281418 1105 28-29 -0.252142434160497 1105 30-31 0.08226338803274302 1105 32-33 0.05984776158401672 1105 34-35 -0.1773232843043857 1105 36-37 -0.1786501000150551 1105 38-39 0.06254547852389436 1105 40-41 0.10925056926572552 1105 42-43 0.12833020931318373 1105 44-45 0.39800421179797496 1105 46-47 0.18209935122435184 1105 48-49 0.16116696656558105 1105 50-51 0.3475211469382842 1105 52-53 0.23487289487528074 1105 54-55 0.15791681958774717 1105 56-57 -0.020930124802376326 1105 58-59 0.09229486858151148 1105 60-61 0.0753571163874085 1105 62-63 0.31580114378387947 1105 64-65 0.29612590479202083 1105 66-67 0.3678293866795954 1105 68-69 0.18073258874231257 1105 70-71 0.022072923329009342 1105 72-73 0.2606261852282934 1105 74-75 -0.033600573681940915 1105 76-77 -0.3241065487214385 1105 78-79 -0.48848721951582164 1105 80-81 -0.2824424515670714 1105 82-83 -0.1697238754662358 1105 84-85 -0.40685906168917896 1105 86-87 -0.28002604466986725 1105 88-89 -0.25392240523805043 1105 90-91 -0.20904404725446568 1105 92-93 -0.3156546988670961 1105 94-95 -0.40534227784340615 1105 96-97 -0.28800839397237255 1105 98-99 -0.2336737131617035 1105 100-101 -0.029279271178708655 1106 1 0.00948229773257836 1106 2 0.1847141191599455 1106 3 0.08095248303020242 1106 4 0.12042615869238915 1106 5 -0.05774216386618036 1106 6 0.08856357818073235 1106 7 -0.013322168288063096 1106 8 -0.10271056522308442 1106 9 0.07694756484828247 1106 10-11 0.029622693121247323 1106 12-13 -0.0011372260673709889 1106 14-15 0.1091724183303171 1106 16-17 6.395274684578567E-4 1106 18-19 -0.023352979454749345 1106 20-21 -0.03284204100540933 1106 22-23 -0.08130178886870709 1106 24-25 -0.18072486092687967 1106 26-27 0.09306436202722779 1106 28-29 -0.005878705712007104 1106 30-31 -0.03246631011919021 1106 32-33 -0.057955280264245346 1106 34-35 -0.031855069282080706 1106 36-37 -0.16622972755627785 1106 38-39 0.042180131617051586 1106 40-41 -0.044238818808679525 1106 42-43 0.15846929716509095 1106 44-45 0.05846831209566261 1106 46-47 0.11337837019272712 1106 48-49 0.1477458524403943 1106 50-51 -0.07935359271648679 1106 52-53 -0.1919205662685144 1106 54-55 -0.4443518668499138 1106 56-57 -0.28576599300927086 1106 58-59 0.09751701291182968 1106 60-61 -0.26239573598033417 1106 62-63 -0.20581386022924875 1106 64-65 -0.2112856794277569 1106 66-67 -0.2514061889246584 1106 68-69 -0.10695270086318232 1106 70-71 0.07260275762244817 1106 72-73 -0.18021321859383121 1106 74-75 -0.3080679454396922 1106 76-77 -0.5714421375334489 1106 78-79 -0.18151860318087287 1106 80-81 -0.11067047842961841 1106 82-83 0.06840692197755516 1106 84-85 -0.38725062091581464 1106 86-87 -0.3121513761337944 1106 88-89 -0.36118972010083894 1106 90-91 -0.08398689830143269 1106 92-93 -0.564946051849514 1106 94-95 -0.5381920040364214 1106 96-97 -0.358365105037425 1106 98-99 -0.035328746841486236 1106 100-101 0.31335029184769425 1107 1 -0.005000177411201179 1107 2 0.10413482015419007 1107 3 0.04397390772705734 1107 4 -0.0735567999305502 1107 5 -0.01633478466016669 1107 6 -0.06264242437311651 1107 7 -0.035228659711783905 1107 8 -0.016038081083557643 1107 9 0.006388245155690697 1107 10-11 0.08493641550650466 1107 12-13 -0.15403035190335856 1107 14-15 0.16535455015335998 1107 16-17 0.034260702171216906 1107 18-19 -0.22004308447248633 1107 20-21 -0.1332465125980633 1107 22-23 -0.22353647336064597 1107 24-25 -0.11706480573949563 1107 26-27 0.10702208539628799 1107 28-29 -0.10265287208947882 1107 30-31 -0.15579982314280016 1107 32-33 0.06002711233430347 1107 34-35 -0.18619189422219762 1107 36-37 -0.11170102708762641 1107 38-39 0.043542127796385444 1107 40-41 -0.027285883062560856 1107 42-43 -0.2961830526784084 1107 44-45 0.07799194192919146 1107 46-47 0.20088136035131043 1107 48-49 -4.6578003458108697E-4 1107 50-51 -0.3330992927842047 1107 52-53 -0.38581592587929947 1107 54-55 -0.02124105746873539 1107 56-57 -0.18594776095948617 1107 58-59 -0.023128726575450287 1107 60-61 0.02832095870678586 1107 62-63 0.009208864976024245 1107 64-65 0.04659065098223891 1107 66-67 0.1437007324080568 1107 68-69 0.14274154489247692 1107 70-71 -0.08156476538071544 1107 72-73 -0.002801634635229533 1107 74-75 0.2016883945388166 1107 76-77 -0.3396880010070191 1107 78-79 -0.00641660086960627 1107 80-81 0.2103756896189779 1107 82-83 0.17290580509015285 1107 84-85 0.248669354017391 1107 86-87 0.34388183736858124 1107 88-89 0.14682474721293914 1107 90-91 0.2242310682559605 1107 92-93 0.23538126522859315 1107 94-95 0.44077833695957835 1107 96-97 0.26115363946657766 1107 98-99 0.676211075332521 1107 100-101 1.009632724539646 1108 1 -0.061560963845000316 1108 2 0.01048469198725499 1108 3 0.04168087802248266 1108 4 0.027989294195833736 1108 5 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-0.6196194610120251 2316 98-99 -0.7357553441106042 2316 100-101 -1.142609363768539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 13.0 5 32.0 6 67.0 7 182.0 8 406.0 9 829.0 10 1325.0 11 2092.0 12 2900.0 13 4159.0 14 5325.0 15 6886.0 16 8768.0 17 11633.0 18 10178.0 19 6594.0 20 5769.0 21 5890.0 22 6372.0 23 7006.0 24 7938.0 25 9456.0 26 11623.0 27 14292.0 28 17520.0 29 21364.0 30 26198.0 31 31649.0 32 39794.0 33 49457.0 34 64856.0 35 87228.0 36 120558.0 37 150667.0 38 87740.0 39 4889.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.54442220340288 18.739654707829704 14.86185426129802 21.854068827469398 2 15.969167498406666 21.00566384877163 38.83103452422468 24.194134128597025 3 20.820226448491145 24.578896231950978 30.358190720806515 24.24268659875136 4 12.980957110511767 16.929618144193043 37.48636744588161 32.60305729941357 5 13.181762979333831 37.997400345578484 33.81775244123422 15.003084233853464 6 31.13298182539635 36.229927073125275 16.93490690250164 15.70218419897674 7 27.93919601373645 33.8433198341622 20.256091460892485 17.961392691208864 8 26.763830237502045 32.59484690785614 19.573116769433515 21.068206085208306 9 26.160770327759646 17.2364203038312 20.93691306156706 35.66589630684209 10-11 25.430438970193027 26.203780191450498 26.364604864300052 22.001175974056427 12-13 27.449835716669124 23.819309378729443 27.06150779932483 21.669347105276607 14-15 23.531165922652598 24.893057371751514 27.35693135530805 24.218845350287836 16-17 21.702008693717797 28.44102280447552 27.759533930149644 22.097434571657036 18-19 21.404883750012026 26.775674076781748 30.57297728868667 21.24646488451956 20-21 22.466680935386744 25.003246534624896 30.425500447300323 22.104572082688033 22-23 21.764494041016373 25.177555046122528 30.846690919847262 22.211259993013833 24-25 22.72177979359031 25.170655338142268 30.685555289684206 21.42200957858322 26-27 22.174016038710313 25.032857831707776 31.51513985471607 21.277986274865835 28-29 21.200841501740303 26.415735288900965 30.934327871829108 21.44909533752962 30-31 21.494950077947784 25.547152546183987 30.686896415724714 22.27100096014352 32-33 21.615191903839758 25.576396900872776 30.74110792535593 22.06730326993153 34-35 22.724624435939912 25.192148704428003 30.427287731523933 21.655939128108155 36-37 22.48594357950333 24.829071160873035 31.039931598400354 21.64505366122328 38-39 22.008204877831737 25.634741638943286 30.970076787699536 21.386976695525437 40-41 21.691892850593856 25.974562387083395 30.696182554402885 21.637362207919868 42-43 21.55369259461 25.983435359303364 29.809018446439673 22.65385359964697 44-45 22.203370673028363 25.536166917575308 29.489721563553633 22.770740845842692 46-47 22.897281386343263 26.315463554320267 29.050430136035892 21.736824923300574 48-49 22.858668225015467 26.409683406290753 28.636522958289518 22.09512541040426 50-51 21.9151085192088 26.69566524379246 29.44604100282571 21.94318523417303 52-53 22.640009763963402 27.243201449684022 28.00724361028559 22.109545176066987 54-55 21.81588076499907 26.497742399009184 28.659387832402437 23.026989003589314 56-57 22.81116810048621 26.49234371026284 28.09520746143297 22.601280727817983 58-59 23.5271165194179 26.12348455015226 27.96868846890341 22.380710461526437 60-61 22.570598693272583 26.143987725155554 29.603322192420933 21.68209138915093 62-63 22.082059705584562 26.68371383839137 29.19226949041262 22.04195696561145 64-65 21.7850226165717 27.238373194176628 28.586047893138428 22.390556296113246 66-67 22.055011871400477 26.78180732677946 28.021916246653372 23.14126455516669 68-69 22.14405489042177 26.560372188997267 28.474346171120118 22.82122674946084 70-71 22.821575266696808 26.599519512875514 28.03166212953433 22.54724309089335 72-73 22.399549323955874 26.2815198171804 28.90525281687558 22.413678041988145 74-75 21.95438705348446 27.508940531934595 28.025050324312232 22.51162209026871 76-77 22.177624692548598 26.703465640896763 28.048886801970337 23.070022864584306 78-79 22.352683000150307 26.741379828648732 28.06373064782805 22.842206523372916 80-81 22.88072004473224 26.918105132783797 27.66003499215382 22.54113983033014 82-83 22.512012755887334 26.666394104971523 28.254567011697706 22.567026127443434 84-85 22.122322055583883 26.91779059364508 28.172949274177256 22.78693807659378 86-87 22.01110842559291 27.30348319727376 27.75224531367053 22.933163063462803 88-89 21.635284865305575 27.16141204878389 27.148905825735405 24.054397260175136 90-91 21.881999248402856 26.472574220217965 27.854731304021048 23.790695227358135 92-93 22.30743517042189 26.6007488799267 28.175765533727255 22.91605041592416 94-95 21.816628468773523 26.520440482231365 28.198221813922952 23.46470923507216 96-97 21.305570652827413 26.429036943692253 28.8304250376363 23.434967365844038 98-99 21.4810632911088 26.923416850493403 27.556814936530927 24.038704921866874 100-101 20.913065096712245 26.236251926218397 28.096183757777776 24.754499219291585 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 224.0 1 179.5 2 240.5 3 316.5 4 230.0 5 210.5 6 346.5 7 635.0 8 1025.0 9 1234.0 10 1136.0 11 992.0 12 962.0 13 1076.5 14 1239.0 15 1378.5 16 1484.0 17 1584.5 18 1756.0 19 1883.5 20 2003.5 21 2248.0 22 2549.0 23 2911.5 24 3383.5 25 4029.5 26 4944.0 27 5980.5 28 6950.0 29 8223.5 30 9785.0 31 11334.0 32 13171.0 33 14337.0 34 15296.5 35 16574.0 36 18117.5 37 19637.0 38 20573.0 39 20995.5 40 21578.5 41 22912.5 42 24017.0 43 25180.5 44 26222.5 45 27240.0 46 28694.5 47 34449.5 48 46754.5 49 45249.5 50 33384.5 51 29017.5 52 27106.0 53 26711.5 54 25726.0 55 23814.5 56 21845.0 57 19907.5 58 18653.5 59 16585.5 60 13944.0 61 11431.0 62 9547.0 63 7536.0 64 5293.0 65 4021.5 66 3148.5 67 2563.0 68 1884.0 69 1215.5 70 921.0 71 781.5 72 677.5 73 590.5 74 494.5 75 439.5 76 347.5 77 220.5 78 137.0 79 94.0 80 77.5 81 61.0 82 43.0 83 27.5 84 17.5 85 12.0 86 7.0 87 3.5 88 2.0 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04328712833190646 2 0.007935973527516186 3 0.003847744740613908 4 8.416941620092923E-4 5 8.416941620092923E-4 6 1.2024202314418463E-4 7 0.0 8 0.0 9 0.0012024202314418462 10-11 6.012101157209231E-4 12-13 0.0016232673124464924 14-15 0.002224477428167415 16-17 0.002525082486027877 18-19 0.0 20-21 0.0 22-23 0.0015030252893023077 24-25 0.0010821782082976615 26-27 0.005170406995199939 28-29 0.005711496099348769 30-31 0.0011422992198697537 32-33 3.0060505786046155E-4 34-35 0.00883778870109757 36-37 0.011603355233413815 38-39 0.009318756793674308 40-41 0.0016232673124464924 42-43 6.012101157209231E-4 44-45 0.0014429042777302154 46-47 6.012101157209231E-5 48-49 7.815731504372E-4 50-51 7.214521388651077E-4 52-53 0.0031864136133208927 54-55 0.002525082486027877 56-57 0.0023447194513116 58-59 0.0016232673124464924 60-61 0.004388833844762738 62-63 0.005050164972055754 64-65 0.00907827274738594 66-67 0.004268591821618554 68-69 0.004268591821618554 70-71 0.0 72-73 0.0019839933818790465 74-75 0.006012101157209231 76-77 0.0024649614744557844 78-79 0.003727502717469723 80-81 0.004929922948911569 82-83 0.004809680925767385 84-85 0.005771617110920862 86-87 0.004208470810046461 88-89 0.008416941620092922 90-91 0.011242629163981261 92-93 0.0016833883240185846 94-95 0.002645324509172062 96-97 0.0014429042777302154 98-99 0.003667381705897631 100-101 0.004749559914195292 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 831656.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.09093436682037 #Duplication Level Percentage of deduplicated Percentage of total 1 83.28466637943946 50.04653421169428 2 10.044616519793784 12.071807840616158 3 2.8301684791951445 5.102024049910777 4 1.2015219346020038 2.8880230284985626 5 0.6327016428788128 1.900981644800508 6 0.3977205531812064 1.433963979452842 7 0.26101690471224065 1.0979324782785702 8 0.18596128745664023 0.8939670015461109 9 0.14041868814729674 0.7594101156000805 >10 0.9006881311189952 10.158960999668238 >50 0.07532556037117412 3.0771985932227146 >100 0.039168063230164946 4.563899703903024 >500 0.0030129279365350057 1.2445353276883202 >1k 0.0028120660740993384 3.983695906347881 >5k 2.0086186243566703E-4 0.7770651187719334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6438 0.7741181450022606 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4775 0.5741556605134815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4295 0.5164394894042729 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 4052 0.48722067778023603 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2883 0.34665775272468424 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2680 0.3222486220264148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2459 0.29567513491154995 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2170 0.26092519022288063 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2043 0.24565445328356916 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1498 0.18012255066998856 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1308 0.15727656627259348 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1276 0.15342882153197956 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1268 0.1524668853468261 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1168 0.14044268303240762 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1130 0.13587348615292863 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 968 0.11639427840357071 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 927 0.11146435545465914 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 858 0.10316765585771041 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 1.2024202314418461E-4 10-11 0.0 0.0 0.0 0.0 1.2024202314418461E-4 12-13 0.0 0.0 0.0 0.0 1.2024202314418461E-4 14-15 0.0 0.0 0.0 0.0 1.2024202314418461E-4 16-17 0.0 0.0 0.0 0.0 1.2024202314418461E-4 18-19 0.0 0.0 0.0 0.0 1.2024202314418461E-4 20-21 0.0 0.0 0.0 6.0121011572092306E-5 1.2024202314418461E-4 22-23 0.0 0.0 0.0 1.8036303471627693E-4 1.2024202314418461E-4 24-25 0.0 0.0 0.0 5.410891041488308E-4 1.2024202314418461E-4 26-27 0.0 0.0 0.0 9.619361851534769E-4 1.2024202314418461E-4 28-29 0.0 0.0 0.0 0.0019238723703069536 1.2024202314418461E-4 30-31 0.0 0.0 0.0 0.004088228786902277 1.2024202314418461E-4 32-33 0.0 0.0 0.0 0.0068537953192185225 1.2024202314418461E-4 34-35 0.0 0.0 0.0 0.010280692978827784 1.2024202314418461E-4 36-37 0.0 0.0 0.0 0.014128437719441693 1.2024202314418461E-4 38-39 0.0 0.0 0.0 0.022425137316390432 1.2024202314418461E-4 40-41 0.0 0.0 0.0 0.036914301105264676 1.2024202314418461E-4 42-43 0.0 0.0 0.0 0.05711496099348769 1.2024202314418461E-4 44-45 0.0 0.0 0.0 0.07911925122887348 1.2024202314418461E-4 46-47 0.0 0.0 0.0 0.12607376126667758 1.2024202314418461E-4 48-49 0.0 0.0 0.0 0.1671965331819887 1.2024202314418461E-4 50-51 0.0 0.0 0.0 0.19737728099117904 1.2024202314418461E-4 52-53 0.0 0.0 0.0 0.23254807276085304 1.2024202314418461E-4 54-55 0.0 0.0 0.0 0.2695224948776898 1.2024202314418461E-4 56-57 0.0 0.0 0.0 0.34894235116442374 1.2024202314418461E-4 58-59 0.0 0.0 0.0 0.43479515568937155 1.2024202314418461E-4 60-61 0.0 0.0 0.0 0.529485748915417 1.8036303471627693E-4 62-63 0.0 0.0 0.0 0.621290533586002 2.4048404628836923E-4 64-65 0.0 0.0 0.0 0.72554036765201 2.4048404628836923E-4 66-67 0.0 0.0 0.0 0.8813139086353012 2.4048404628836923E-4 68-69 0.0 0.0 0.0 1.074542839828006 2.4048404628836923E-4 70-71 0.0 0.0 0.0 1.2077710014717624 2.4048404628836923E-4 72-73 0.0 0.0 0.0 1.319596082995854 3.6072606943255385E-4 74-75 0.0 0.0 0.0 1.4546879959983454 3.6072606943255385E-4 76-77 0.0 0.0 0.0 1.6108222630510691 3.6072606943255385E-4 78-79 0.0 0.0 0.0 1.7808444837769462 3.6072606943255385E-4 80-81 0.0 0.0 0.0 2.0461585078445896 3.6072606943255385E-4 82-83 0.0 0.0 0.0 2.3583669209384652 3.6072606943255385E-4 84-85 0.0 0.0 0.0 2.760997335436767 3.6072606943255385E-4 86-87 0.0 0.0 0.0 3.151242821551218 3.6072606943255385E-4 88-89 0.0 0.0 0.0 3.6105673499620035 3.6072606943255385E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGTACT 4590 0.0 23.18041 12-13 TACCTGG 670 0.0 21.979197 2 GTACATG 4275 0.0 21.564892 1 GTACTTT 5000 0.0 21.326475 14-15 GGCTCGT 115 0.0037208688 20.653664 6 CTATACC 115 0.0037221652 20.652424 4 GTATAGG 210 8.566112E-7 20.36596 1 TACATGG 4400 0.0 20.188957 2 ACATGGG 4225 0.0 20.012075 3 AGTACTT 4805 0.0 19.869568 12-13 GCCACGT 265 1.8513674E-8 19.718405 8 GTGTATC 195 1.0282325E-5 19.495619 1 ACTTTTT 5700 0.0 19.082417 16-17 CAATTGG 125 0.006014709 19.008228 1 GTACCTG 760 0.0 18.75812 1 GCTATCG 65 0.0075653004 18.268902 20-21 ATGGGAG 1045 0.0 18.182037 5 CATGGGA 2520 0.0 17.906961 4 AGTACAT 3490 0.0 17.8308 2 AGAGTAC 8200 0.0 17.494112 10-11 >>END_MODULE