FastQCFastQC Report
Thu 26 May 2016
SRR936448_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936448_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904925
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT66110.7305577810315772No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT39750.4392629223416305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33390.3689808547669696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.31317512501035993No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT26480.29262093543663836No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC25570.2825648534408929No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT20240.2236649446086692No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15810.17471061137663343No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT15700.17349504102549934No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14880.16443351658977262No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.16189186949194687No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG13200.14586844213608863No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12330.1362543857225737No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC11980.1323866618780562No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA11460.12664032930905875No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA10570.11680526010442854No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA10180.11249551067768049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG6850.027.0697382
GTACATG49900.025.4444371
TACATGG50200.024.0569232
GTACCTG8250.023.632631
ACATGGG49600.023.3906463
CATGGGG20200.022.8338154
GAGTACT33100.021.69057312-13
TAGCCCT4550.020.9014644
GTTCCGT1404.815776E-420.3789276
AGTACTT34850.020.12386112-13
ATACCGT1200.00473334919.8128456
GTATAGC2651.8295395E-819.7391741
GTACTTT36950.019.48839614-15
GGACGCT2952.9740477E-919.342716
GTATATG3455.0931703E-1119.2970581
GGTGCGT1250.00598537219.0245448
GTCTACT1759.8483935E-519.0213851
GTGTAGC4750.019.0213831
CTGGACG3254.802132E-1019.0203324
TAAGGTG4300.018.8002055
Produced by FastQC (version 0.11.5)