##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936448_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 904925 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.247727712241346 31.0 31.0 34.0 28.0 34.0 2 31.28484902063707 31.0 31.0 34.0 28.0 34.0 3 31.209319004337377 31.0 31.0 34.0 27.0 34.0 4 34.87178384949029 37.0 35.0 37.0 32.0 37.0 5 34.91138271127441 37.0 35.0 37.0 32.0 37.0 6 34.98050667182363 37.0 35.0 37.0 32.0 37.0 7 34.83061469182529 37.0 35.0 37.0 32.0 37.0 8 34.822875928944384 37.0 35.0 37.0 32.0 37.0 9 36.26713705555709 39.0 37.0 39.0 32.0 39.0 10-11 36.15449291377739 39.0 35.0 39.0 31.5 39.0 12-13 36.07008315606265 39.0 35.0 39.0 30.5 39.0 14-15 37.01021410614139 39.0 36.0 41.0 31.0 41.0 16-17 36.94681990220184 39.0 36.0 41.0 31.0 41.0 18-19 36.8480868580269 39.0 36.0 41.0 30.0 41.0 20-21 36.85925242423406 39.0 36.0 41.0 30.0 41.0 22-23 36.85785838605409 39.0 36.0 41.0 30.5 41.0 24-25 36.9580274608393 39.0 36.0 41.0 31.0 41.0 26-27 36.87411221924469 39.0 36.0 41.0 30.0 41.0 28-29 36.76720114926651 39.0 36.0 41.0 30.0 41.0 30-31 36.57954802884217 39.0 36.0 41.0 30.0 41.0 32-33 36.409582562090776 39.0 35.0 40.5 30.0 41.0 34-35 36.200248639390004 39.0 35.0 40.0 30.0 41.0 36-37 35.96765698814819 38.5 35.0 40.0 29.0 41.0 38-39 35.76790673260215 38.0 35.0 40.0 28.0 41.0 40-41 35.57337403652237 38.0 35.0 40.0 27.5 41.0 42-43 35.27035610685968 38.0 34.0 40.0 26.5 41.0 44-45 35.08483189214576 38.0 34.0 40.0 26.0 41.0 46-47 34.90040390087576 38.0 34.0 40.0 24.5 41.0 48-49 34.61634444843495 38.0 34.0 40.0 24.0 41.0 50-51 33.59213802248805 37.0 32.5 39.5 22.5 40.5 52-53 33.698747410006355 37.5 33.0 39.5 23.0 40.5 54-55 34.1466143603061 38.0 33.0 40.0 23.0 41.0 56-57 33.968944387656435 38.0 33.0 40.0 22.0 41.0 58-59 33.732016465452936 37.5 33.0 40.0 21.5 41.0 60-61 33.39127496753875 37.0 33.0 40.0 20.0 41.0 62-63 33.07400834323286 36.5 32.0 40.0 19.5 41.0 64-65 32.706369588639944 36.0 32.0 39.0 18.0 41.0 66-67 32.312901621681355 35.5 31.0 39.0 17.5 40.5 68-69 31.874125480012154 35.0 31.0 38.5 13.0 40.0 70-71 31.451661739923196 35.0 31.0 38.0 11.0 40.0 72-73 31.03477415255408 35.0 30.5 37.0 8.0 39.5 74-75 30.63551841312816 35.0 30.0 37.0 7.0 39.0 76-77 30.185316462690277 34.0 29.5 36.0 4.0 39.0 78-79 29.698687736552753 34.0 29.0 36.0 2.0 37.5 80-81 29.234616128408433 34.0 29.0 35.0 2.0 37.0 82-83 28.83588032157361 34.0 29.0 35.0 2.0 37.0 84-85 28.475881426637564 34.0 28.0 35.0 2.0 36.0 86-87 28.15474155316739 34.0 27.0 35.0 2.0 36.0 88-89 27.770976047738763 34.0 26.5 35.0 2.0 35.5 90-91 27.403283697543998 33.0 26.0 35.0 2.0 35.0 92-93 26.975934469707433 33.0 25.0 35.0 2.0 35.0 94-95 26.487974694035415 33.0 25.0 35.0 2.0 35.0 96-97 25.867847611680524 33.0 23.5 35.0 2.0 35.0 98-99 25.144057242312897 32.5 19.0 35.0 2.0 35.0 100-101 22.70460645909882 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5703388965554943 1101 2 0.6256011181714527 1101 3 0.605219246662088 1101 4 0.6900454597339376 1101 5 0.6657953630540447 1101 6 0.6448498929751594 1101 7 0.5975632734676637 1101 8 0.5445245999434363 1101 9 0.7421284896131226 1101 10-11 0.7217623938823579 1101 12-13 0.9117412911227092 1101 14-15 1.157944206912525 1101 16-17 1.178375139398348 1101 18-19 1.1382302853733464 1101 20-21 1.0449524256068017 1101 22-23 1.0309159133526649 1101 24-25 0.8397384390775287 1101 26-27 0.8179388432519517 1101 28-29 0.8969151644140183 1101 30-31 0.7997962940208296 1101 32-33 0.8206531678835915 1101 34-35 0.949722017671597 1101 36-37 0.8963307540532739 1101 38-39 0.9227560039428866 1101 40-41 0.6501764213318637 1101 42-43 0.9134187071847251 1101 44-45 0.873906428605153 1101 46-47 0.9941519844520528 1101 48-49 1.1512602186665006 1101 50-51 1.045174392555218 1101 52-53 1.1937906116002708 1101 54-55 1.086617230734241 1101 56-57 1.217019527691022 1101 58-59 1.1600451595501582 1101 60-61 1.0681241505297834 1101 62-63 1.1982174823566893 1101 64-65 1.0911557230745714 1101 66-67 0.884759607513935 1101 68-69 1.0126144525667655 1101 70-71 1.031745822744913 1101 72-73 0.9566744262312383 1101 74-75 0.9164367743366775 1101 76-77 1.0171509314424263 1101 78-79 0.9759456443269912 1101 80-81 1.056322868479274 1101 82-83 1.123032881685976 1101 84-85 1.1148639230911925 1101 86-87 1.1950132564283535 1101 88-89 1.1620579002498772 1101 90-91 1.223059904988233 1101 92-93 1.4386823568240281 1101 94-95 1.5228464193500386 1101 96-97 1.203831993418138 1101 98-99 1.2895502588428123 1101 100-101 1.5086111020691888 1102 1 0.35776186574317137 1102 2 0.5176752811699856 1102 3 0.5993662387922747 1102 4 0.5459064708050292 1102 5 0.36233664623398454 1102 6 0.38393686109574787 1102 7 0.5235810138971644 1102 8 0.4043025111079004 1102 9 0.5673112293756972 1102 10-11 0.4892213774758005 1102 12-13 0.6037214165062039 1102 14-15 0.8598789808220957 1102 16-17 0.5693918794276911 1102 18-19 0.6941719953906826 1102 20-21 0.7654456228857143 1102 22-23 0.5667672538888837 1102 24-25 0.6724888725842604 1102 26-27 0.5607329608990099 1102 28-29 0.40807229393248434 1102 30-31 0.4651247587400533 1102 32-33 0.49852031474233627 1102 34-35 0.13610957269360568 1102 36-37 0.2703877101690608 1102 38-39 0.14838192097636949 1102 40-41 0.45097407372615805 1102 42-43 0.1880815723307805 1102 44-45 0.6487803781849806 1102 46-47 0.5152884390338883 1102 48-49 0.46642961976026953 1102 50-51 0.38230057636207704 1102 52-53 0.23739805457746144 1102 54-55 0.5243039720974565 1102 56-57 0.3764896490729086 1102 58-59 0.18773423518041454 1102 60-61 0.15796808810481622 1102 62-63 0.3745103328302122 1102 64-65 0.4083949520995205 1102 66-67 0.35264943010963634 1102 68-69 0.4324503869405163 1102 70-71 0.09912043925818992 1102 72-73 0.4599227255509639 1102 74-75 0.4176832729361166 1102 76-77 0.5981806766738522 1102 78-79 0.44339729165258746 1102 80-81 0.7407703141242017 1102 82-83 0.37256402747762607 1102 84-85 0.4253861319747472 1102 86-87 0.37174795030590246 1102 88-89 0.4279189113209725 1102 90-91 0.7762518484322811 1102 92-93 0.5305180911177452 1102 94-95 0.6200646399716199 1102 96-97 0.5025031285388515 1102 98-99 0.5247863532805859 1102 100-101 0.7877894400710552 1103 1 0.3230127687359463 1103 2 0.40774666059894926 1103 3 0.5809785108940986 1103 4 0.528996856081946 1103 5 0.4800213297565179 1103 6 0.4070061765412234 1103 7 0.5844147772270603 1103 8 0.47817054516362845 1103 9 0.7603958096990553 1103 10-11 0.5024598965676432 1103 12-13 0.5312750585770658 1103 14-15 0.8828730468695625 1103 16-17 0.8210070540062944 1103 18-19 0.8641791978330673 1103 20-21 0.8356210614822004 1103 22-23 0.7434474654471899 1103 24-25 0.4981091426220985 1103 26-27 0.7057842379887944 1103 28-29 0.6747991601175656 1103 30-31 0.6318344250627277 1103 32-33 0.5726140969920763 1103 34-35 0.5268151970486059 1103 36-37 0.30884015254952146 1103 38-39 0.22497237075276644 1103 40-41 0.4150505486143601 1103 42-43 0.5356934789355279 1103 44-45 0.7308237218382345 1103 46-47 0.9084934183940518 1103 48-49 0.9402455167330928 1103 50-51 0.6806859392791793 1103 52-53 0.7825073812028478 1103 54-55 0.4415161968996557 1103 56-57 0.698288324683503 1103 58-59 0.5042251407531637 1103 60-61 0.618470216587788 1103 62-63 0.6020349501332802 1103 64-65 0.5440400544387032 1103 66-67 0.4766792503710775 1103 68-69 0.5627070948868536 1103 70-71 0.4940803420789628 1103 72-73 0.48569026941061466 1103 74-75 0.4018244623066032 1103 76-77 0.298502636598176 1103 78-79 0.27853392305953406 1103 80-81 0.5578632148809923 1103 82-83 0.4670584590221729 1103 84-85 0.6915190654112777 1103 86-87 0.5008597924111662 1103 88-89 0.35467705975578667 1103 90-91 0.39408165579381915 1103 92-93 0.4441613444717163 1103 94-95 0.3706720101126564 1103 96-97 0.35116852671566434 1103 98-99 0.5057186933218638 1103 100-101 0.9357727562045284 1104 1 0.2204069237663795 1104 2 0.2795466964027433 1104 3 0.436817886117872 1104 4 0.7046032828631894 1104 5 0.5754552269996225 1104 6 0.45223084535611235 1104 7 0.5965768789098362 1104 8 0.5641164366781268 1104 9 0.7524686256675466 1104 10-11 0.7057079721136503 1104 12-13 0.6886970734356339 1104 14-15 0.6364646150757949 1104 16-17 0.5619805815752201 1104 18-19 0.6868357275502177 1104 20-21 0.9261599085999919 1104 22-23 0.6271914235567522 1104 24-25 0.7713200717305853 1104 26-27 0.37008170039481314 1104 28-29 0.40683013040040805 1104 30-31 0.3340139810956657 1104 32-33 0.5683232359107961 1104 34-35 0.4656914054266892 1104 36-37 0.4664327948696041 1104 38-39 0.3731301536027516 1104 40-41 0.4849043124883252 1104 42-43 0.7609357139874433 1104 44-45 0.8529370408500512 1104 46-47 0.8012778347921952 1104 48-49 0.7691173615236409 1104 50-51 0.72032745377971 1104 52-53 0.7374980945934695 1104 54-55 0.9834709722308403 1104 56-57 0.9754208882352344 1104 58-59 0.9038206697542392 1104 60-61 0.859773810393726 1104 62-63 0.9833705435811808 1104 64-65 0.987618308108587 1104 66-67 0.9661031449289013 1104 68-69 0.7091110512062215 1104 70-71 0.7505893601598821 1104 72-73 0.9127288479999471 1104 74-75 0.9472701076700112 1104 76-77 0.8418448089934465 1104 78-79 0.526438841605902 1104 80-81 0.9129725283432215 1104 82-83 1.0289682558356361 1104 84-85 1.0101700455401712 1104 86-87 0.9669860455439974 1104 88-89 0.9926531383451191 1104 90-91 1.1284510614508214 1104 92-93 0.7898218126063377 1104 94-95 0.7624382560847316 1104 96-97 0.6639510410371834 1104 98-99 0.9056386942169574 1104 100-101 1.3304648435657853 1105 1 0.15287038109944717 1105 2 0.2709039694989812 1105 3 0.3641361420043765 1105 4 0.4296140351605473 1105 5 0.3227732102926737 1105 6 0.11014801934948792 1105 7 0.29521497172488154 1105 8 0.27609419305227334 1105 9 0.29548084391192475 1105 10-11 0.3644330828168876 1105 12-13 0.43919872111162306 1105 14-15 0.45683347945146124 1105 16-17 0.5164499026963654 1105 18-19 0.5774205010856335 1105 20-21 0.3531756614728252 1105 22-23 0.3234075992523202 1105 24-25 0.07502438961089553 1105 26-27 0.2661668040711831 1105 28-29 0.2637409045052479 1105 30-31 0.2044518267593105 1105 32-33 0.34902142346721376 1105 34-35 0.12123890937930071 1105 36-37 -5.856687936827143E-4 1105 38-39 -0.16767632927535203 1105 40-41 -0.007004962352510802 1105 42-43 -0.3625301906290801 1105 44-45 0.008779854377955587 1105 46-47 0.34886621564765363 1105 48-49 0.5085793529096918 1105 50-51 0.5229391262314138 1105 52-53 0.6482002650431227 1105 54-55 0.428393086223366 1105 56-57 0.4040415350692186 1105 58-59 0.509044014657654 1105 60-61 0.43574295584354417 1105 62-63 0.7065075944834618 1105 64-65 0.5383358346561593 1105 66-67 0.47924631403982687 1105 68-69 0.46824823234760515 1105 70-71 0.6409053262613718 1105 72-73 0.28707840973024545 1105 74-75 0.2896539222822305 1105 76-77 0.37993065775859947 1105 78-79 0.49990224744909284 1105 80-81 0.24946150951004142 1105 82-83 0.2659220088417378 1105 84-85 0.21555558354878457 1105 86-87 0.17139176152584668 1105 88-89 0.24996129312973636 1105 90-91 0.2790774963524747 1105 92-93 0.459170117377937 1105 94-95 0.5580204248382543 1105 96-97 0.49990846496846686 1105 98-99 0.5515769003097297 1105 100-101 1.1928115854682488 1106 1 0.18967204917138147 1106 2 0.1110760823506709 1106 3 0.14406368766429622 1106 4 0.013701115544648701 1106 5 0.18180744530630477 1106 6 0.22156599877231997 1106 7 0.23818674866419087 1106 8 0.34429302344186397 1106 9 0.17291422326142225 1106 10-11 0.43877861972753607 1106 12-13 0.45510766993230334 1106 14-15 0.32042610289672524 1106 16-17 0.08669369262609194 1106 18-19 0.45874610460426624 1106 20-21 0.11953322566876068 1106 22-23 -0.04846835648429959 1106 24-25 -0.32867073104213773 1106 26-27 -0.24441409792451907 1106 28-29 -0.11472445917735996 1106 30-31 -0.10968285247647458 1106 32-33 0.11079102947984154 1106 34-35 0.20604157775369458 1106 36-37 0.01076827099554123 1106 38-39 -0.01993190993234606 1106 40-41 0.10904913170633534 1106 42-43 0.09039814412078329 1106 44-45 -0.12968261991748875 1106 46-47 0.15112019108955366 1106 48-49 0.09027001613387142 1106 50-51 -0.13664840107836085 1106 52-53 0.04503562573495401 1106 54-55 0.22901354409830077 1106 56-57 0.369044466698881 1106 58-59 0.2605520719925565 1106 60-61 0.37428687368355895 1106 62-63 0.2170018993492917 1106 64-65 0.0778156773788794 1106 66-67 7.097294982116864E-4 1106 68-69 0.0015522212226173338 1106 70-71 0.01540496382069989 1106 72-73 -0.08043938703600517 1106 74-75 -0.05045398196584472 1106 76-77 0.10761563345226222 1106 78-79 0.1377099629576648 1106 80-81 0.3054577482376324 1106 82-83 0.37907362056217053 1106 84-85 0.27002560066544845 1106 86-87 0.27260184024897427 1106 88-89 0.647136705965714 1106 90-91 0.890789061166835 1106 92-93 0.7912312473479766 1106 94-95 0.9020328035811502 1106 96-97 0.8016149775795434 1106 98-99 1.0014625076132866 1106 100-101 1.3199420860756916 1107 1 0.12614877440998384 1107 2 0.12366575666625579 1107 3 0.1094495781999072 1107 4 0.39404894960571824 1107 5 0.4384840816641642 1107 6 0.23753102490749 1107 7 0.39813151563441807 1107 8 0.3046141227414054 1107 9 0.5669680313777832 1107 10-11 0.49973599224364307 1107 12-13 0.3300194945974084 1107 14-15 0.10084042116030645 1107 16-17 0.23652474868443818 1107 18-19 0.41464785611911026 1107 20-21 0.4335837889328005 1107 22-23 0.346365803345833 1107 24-25 0.2669624736727769 1107 26-27 0.05661914064972251 1107 28-29 0.18529123603224917 1107 30-31 0.059967373605083196 1107 32-33 0.1837561191012469 1107 34-35 0.15940299787036594 1107 36-37 -0.011651189653569816 1107 38-39 -0.0026601253341027586 1107 40-41 0.021247660236348054 1107 42-43 -0.3217906851223802 1107 44-45 -0.17589707643999475 1107 46-47 -0.26395020809782466 1107 48-49 -0.23458114390083296 1107 50-51 -0.3221816502336381 1107 52-53 -0.04231438802039378 1107 54-55 -0.14102482980744213 1107 56-57 0.08971735031449413 1107 58-59 0.18357941340039474 1107 60-61 0.0944977185443463 1107 62-63 -0.008023318796482215 1107 64-65 0.002235307324632174 1107 66-67 0.20202195483204832 1107 68-69 0.03166497984087613 1107 70-71 -0.034691852697008585 1107 72-73 -0.14006178498187083 1107 74-75 0.04313884549971547 1107 76-77 0.2177836603704293 1107 78-79 0.3523040389859666 1107 80-81 0.27313267046698186 1107 82-83 -0.029855366550119555 1107 84-85 0.03382151356232299 1107 86-87 0.18819776443229586 1107 88-89 0.1264331373335601 1107 90-91 -0.0906632571390702 1107 92-93 -0.16388838894640045 1107 94-95 -0.18972774042539697 1107 96-97 0.2897492227634082 1107 98-99 0.42051490492429267 1107 100-101 0.8226711126905357 1108 1 -0.06361564205657544 1108 2 -0.02765279034664303 1108 3 0.10860541040050364 1108 4 0.19098350407706732 1108 5 0.2225524220420212 1108 6 0.2342238849323266 1108 7 0.5164432975531668 1108 8 0.3439061186204526 1108 9 -0.12032264047009988 1108 10-11 0.12435846708508791 1108 12-13 0.015528306455713903 1108 14-15 0.3580633441353811 1108 16-17 0.43004972704162725 1108 18-19 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2316 96-97 -4.975811388724242 2316 98-99 -6.943451783882605 2316 100-101 -7.7501086627310265 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2632.0 3 913.0 4 994.0 5 1012.0 6 1077.0 7 1294.0 8 1644.0 9 2068.0 10 2653.0 11 3181.0 12 4191.0 13 4954.0 14 5620.0 15 6824.0 16 8335.0 17 10238.0 18 8257.0 19 6551.0 20 6552.0 21 7019.0 22 7940.0 23 8810.0 24 10065.0 25 11565.0 26 13320.0 27 15842.0 28 18650.0 29 22256.0 30 27203.0 31 32807.0 32 40478.0 33 50752.0 34 66237.0 35 89558.0 36 120952.0 37 152634.0 38 113843.0 39 15997.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.628231858730096 18.339426049541803 14.034189263177687 21.998152828550413 2 15.627901937720548 20.77787407911843 39.19948527916088 24.39473870400014 3 20.770443171167347 24.662891046386193 29.52378110244575 25.04288468000071 4 12.747989335408477 16.371385998965376 37.444123642055274 33.43650102357087 5 13.592889832194736 38.088589684066235 33.436360018571335 14.882160465167695 6 31.357655099069497 35.878934340960924 17.314693107309907 15.448717452659672 7 28.517847089416293 33.34067030418196 20.264750353114767 17.87673225328698 8 26.74529830222459 32.85432615909968 19.55797903574142 20.84239650293431 9 26.41596037777377 17.664540518659187 20.84281288607172 35.076686217495315 10-11 25.44201752974477 26.246178397307336 26.468576458872118 21.843227614075776 12-13 27.394493891315165 23.884626015810714 26.79014870916026 21.93073138371386 14-15 23.457089779173533 25.254995410967236 26.535114394081806 24.752800415777426 16-17 22.071474782550997 28.720133593590162 26.828755799100897 22.379635824757944 18-19 21.858626295243678 27.599831908611367 28.97193316155573 21.569608634589226 20-21 23.250921004201306 25.944298727008054 28.643258293459073 22.161521975331567 22-23 22.45206527169276 26.12669269163944 28.92999562320673 22.49124641346107 24-25 23.040900830794673 26.237625404012043 28.838446029296676 21.88302773589661 26-27 22.409052295655414 25.989148343373074 29.687355780577008 21.914443580394504 28-29 21.65537766490618 27.476203579989456 29.106560882697156 21.7618578724072 30-31 22.017095528309287 26.426404689943254 28.89298994933226 22.663509832415198 32-33 21.886153936270563 26.68009122362221 29.030917557784264 22.402837282322967 34-35 23.242221066656203 26.2663717210277 28.57202065208922 21.91938656022688 36-37 22.87212351679392 25.94081431702069 29.16891834395904 22.01814382222635 38-39 22.292321719669552 26.70394827729119 29.167352803028212 21.83637720001105 40-41 22.45575471849287 26.757751523560554 28.85794155075229 21.928552207194286 42-43 22.004444694932804 27.02892809031726 28.023403716878903 22.94322349787103 44-45 22.439080182867198 26.59039096316908 28.01736951042027 22.953159343543454 46-47 23.144115661180724 27.276529722549753 27.528762242808476 22.05059237346105 48-49 22.992236317414218 27.202615484156862 27.415010302032723 22.390137896396194 50-51 22.1344790775698 27.568034089427286 28.089022824268383 22.208464008734534 52-53 22.868979677310097 27.89149813050702 26.999545265931026 22.23997692625186 54-55 22.056529702819855 27.22205492735566 27.526777154949734 23.194638214874754 56-57 22.83768954233559 27.407813437627432 27.104678315395354 22.649818704641625 58-59 23.622740759977674 26.868255432950782 26.868255432950782 22.640748374120754 60-61 22.665596159435587 27.150067357965312 28.264485277946928 21.919851204652176 62-63 22.390453788718908 27.455192736021022 27.91794926199313 22.23640421326694 64-65 22.05334937892403 28.11321202999376 27.411714717795967 22.421723873286243 66-67 22.22473318142337 27.651057218191184 26.89729059521152 23.226919005173926 68-69 22.072198511648878 27.48835775239973 27.42591949996353 23.013524235987862 70-71 22.712888957650136 27.53020353021458 26.94958849157782 22.80731902055746 72-73 22.65628626820733 26.983767317550882 27.792527616340116 22.567418797901666 74-75 22.013040876111678 28.56619441062678 26.93069678995944 22.4900679233021 76-77 22.415038529793925 27.527731890333644 26.93454494608371 23.12268463378872 78-79 22.581199594796463 27.759426702205797 27.00682689485985 22.652546808137895 80-81 22.834294960176436 27.870802887035694 26.7619433390786 22.532958813709275 82-83 22.590142085609425 27.648398622133175 27.301666414330345 22.459792877927054 84-85 22.37442644835604 27.940584912165928 27.028980382102535 22.656008257375497 86-87 22.551285571966666 27.98028750973165 26.78978040321204 22.67864651508965 88-89 22.190487714988393 27.92143029615865 26.32672986677585 23.561352122077107 90-91 22.314250523150406 27.502930029082023 26.884322286069196 23.29849716169837 92-93 22.87788763558155 27.8809490653245 26.894715552284136 22.346447746809815 94-95 22.339083947023177 27.644447833876562 27.017605520942823 22.99886269815744 96-97 21.77384192420872 27.63853390989597 27.512482212674243 23.07514195322106 98-99 22.099297584905784 28.128051891060167 26.357450745552637 23.415199778481416 100-101 22.006784599467665 27.45983181090665 26.878255237118324 23.65512835250736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 248.0 1 173.5 2 155.5 3 221.5 4 213.5 5 216.5 6 303.0 7 474.0 8 718.5 9 909.5 10 912.5 11 859.0 12 885.0 13 969.5 14 1102.0 15 1181.5 16 1199.5 17 1278.5 18 1385.5 19 1532.5 20 1711.0 21 1934.0 22 2261.0 23 2727.0 24 3235.5 25 3840.0 26 4818.5 27 6012.0 28 7213.5 29 8547.0 30 10087.0 31 11987.5 32 14265.0 33 15628.5 34 16857.5 35 18197.5 36 19601.5 37 21444.0 38 22512.5 39 23030.5 40 23518.5 41 25051.0 42 26622.5 43 27767.5 44 29052.5 45 30758.0 46 32211.5 47 37134.5 48 49346.0 49 48937.5 50 37409.5 51 32225.5 52 29693.0 53 29373.5 54 29300.0 55 27161.0 56 24644.0 57 22266.5 58 20438.0 59 18304.5 60 15433.0 61 12864.0 62 10742.0 63 8272.0 64 5937.5 65 4730.0 66 3581.5 67 2683.0 68 2062.0 69 1484.5 70 1108.5 71 878.5 72 734.0 73 661.0 74 536.5 75 389.5 76 294.0 77 198.5 78 119.5 79 73.5 80 60.0 81 42.5 82 26.0 83 16.0 84 12.5 85 11.0 86 8.5 87 9.5 88 5.0 89 2.5 90 2.5 91 1.5 92 0.5 93 0.5 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09282537226842004 2 0.039450783214078515 3 0.01911760643147222 4 0.027516092493853082 5 0.03370445064508108 6 0.03956128960963616 7 0.09149929552172832 8 0.06895599082796916 9 0.08685802690830732 10-11 0.07630466613255242 12-13 0.05249053788988038 14-15 0.06575130535679752 16-17 0.07624941293477361 18-19 0.0723816890902561 20-21 0.02276431748487444 22-23 0.016907478520319364 24-25 0.027516092493853082 26-27 0.029891979998342404 28-29 0.00685139652457386 30-31 0.001105063955576429 32-33 0.012763488686907756 34-35 0.013868552642484183 36-37 0.004862281404536288 38-39 0.01105063955576429 40-41 0.006022598557891538 42-43 0.0028731662844987153 44-45 6.630383733458574E-4 46-47 6.630383733458574E-4 48-49 7.182915711246788E-4 50-51 0.001215570351134072 52-53 5.525319777882145E-5 54-55 2.7626598889410727E-4 56-57 0.005304306986766859 58-59 0.0033151918667292868 60-61 0.006409370942343288 62-63 0.0023758875044893224 64-65 3.8677238445175013E-4 66-67 3.315191866729287E-4 68-69 0.00342569826228693 70-71 0.0024863939000469653 72-73 0.02287482388043208 74-75 0.01707323811365583 76-77 0.026024256153824907 78-79 0.021493493935961543 80-81 0.013426527060253612 82-83 0.005304306986766859 84-85 0.004088736635632788 86-87 0.0018233555267011079 88-89 0.0023758875044893224 90-91 0.007790700886813825 92-93 0.013150261071359503 94-95 0.016796972124761722 96-97 0.0151946293891759 98-99 0.028786916042765977 100-101 0.025305964582700225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 904925.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.965841572298686 #Duplication Level Percentage of deduplicated Percentage of total 1 84.34613594794091 53.10925433333615 2 9.669916870504942 12.17748907371025 3 2.521704987035191 4.763438301172 4 1.067930814397772 2.6897264987818406 5 0.5592177528835719 1.7605808216241934 6 0.3467962018270941 1.3101788821271836 7 0.24403181434615528 1.0755967992504434 8 0.17714117368956828 0.892307446277472 9 0.12799919362715692 0.7253619252578593 >10 0.8374738118266525 9.868721345109362 >50 0.06375587852978919 2.714051224602752 >100 0.032228928650857705 3.7496734185322866 >500 0.002656230347058574 1.03093996706537 >1k 0.002833312370195812 3.395545621570594 >5k 1.7708202313723826E-4 0.7371343415822151 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6611 0.7305577810315772 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3975 0.4392629223416305 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3339 0.3689808547669696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2834 0.31317512501035993 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2648 0.29262093543663836 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2557 0.2825648534408929 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2024 0.2236649446086692 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1581 0.17471061137663343 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1570 0.17349504102549934 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1488 0.16443351658977262 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.16189186949194687 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1320 0.14586844213608863 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1233 0.1362543857225737 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1198 0.1323866618780562 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1146 0.12664032930905875 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1057 0.11680526010442854 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1018 0.11249551067768049 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 5.525319777882145E-5 16-17 0.0 0.0 0.0 0.0 1.105063955576429E-4 18-19 0.0 0.0 0.0 5.525319777882145E-5 1.105063955576429E-4 20-21 0.0 0.0 0.0 1.105063955576429E-4 1.105063955576429E-4 22-23 0.0 0.0 0.0 1.105063955576429E-4 1.105063955576429E-4 24-25 1.105063955576429E-4 0.0 0.0 3.315191866729287E-4 1.105063955576429E-4 26-27 1.105063955576429E-4 0.0 0.0 5.525319777882145E-4 1.105063955576429E-4 28-29 1.6575959333646435E-4 0.0 0.0 0.0014365831422493576 1.105063955576429E-4 30-31 2.210127911152858E-4 0.0 0.0 0.0042544962289692515 1.105063955576429E-4 32-33 2.210127911152858E-4 0.0 0.0 0.006298864546785646 1.105063955576429E-4 34-35 2.210127911152858E-4 0.0 0.0 0.008951018040169074 1.105063955576429E-4 36-37 2.210127911152858E-4 0.0 0.0 0.012376716302456004 1.105063955576429E-4 38-39 2.210127911152858E-4 0.0 0.0 0.019172859629251044 1.105063955576429E-4 40-41 2.210127911152858E-4 0.0 0.0 0.030610271569467086 1.105063955576429E-4 42-43 2.210127911152858E-4 0.0 0.0 0.05271155068099566 1.105063955576429E-4 44-45 2.210127911152858E-4 0.0 0.0 0.07249219548581373 1.105063955576429E-4 46-47 2.210127911152858E-4 0.0 0.0 0.115423930159958 1.105063955576429E-4 48-49 2.210127911152858E-4 0.0 0.0 0.15498521976959417 1.105063955576429E-4 50-51 2.210127911152858E-4 0.0 0.0 0.18609277011907066 1.105063955576429E-4 52-53 2.210127911152858E-4 0.0 0.0 0.22046025913749756 1.105063955576429E-4 54-55 2.210127911152858E-4 0.0 0.0 0.25510401414481865 1.105063955576429E-4 56-57 2.210127911152858E-4 0.0 0.0 0.33455811255076384 1.105063955576429E-4 58-59 3.315191866729287E-4 0.0 0.0 0.41511727491228556 1.105063955576429E-4 60-61 3.315191866729287E-4 0.0 0.0 0.4983838439649695 2.210127911152858E-4 62-63 3.315191866729287E-4 0.0 0.0 0.5825897173798933 2.210127911152858E-4 64-65 3.315191866729287E-4 0.0 0.0 0.6752493300549769 2.210127911152858E-4 66-67 3.315191866729287E-4 0.0 0.0 0.8205652402132774 2.210127911152858E-4 68-69 3.315191866729287E-4 0.0 0.0 0.9987015498521976 2.210127911152858E-4 70-71 3.315191866729287E-4 0.0 0.0 1.1299278945768987 2.210127911152858E-4 72-73 3.315191866729287E-4 0.0 0.0 1.2414841008923392 2.210127911152858E-4 74-75 3.315191866729287E-4 0.0 0.0 1.3707765836947814 2.210127911152858E-4 76-77 3.315191866729287E-4 0.0 0.0 1.5187446473464652 2.210127911152858E-4 78-79 3.315191866729287E-4 0.0 0.0 1.6810232892228638 2.210127911152858E-4 80-81 3.315191866729287E-4 0.0 0.0 1.9376191397077105 2.7626598889410727E-4 82-83 3.315191866729287E-4 0.0 0.0 2.230074315551012 4.420255822305716E-4 84-85 3.315191866729287E-4 0.0 0.0 2.6041937177114125 4.420255822305716E-4 86-87 3.315191866729287E-4 0.0 0.0 2.980910020167417 4.420255822305716E-4 88-89 3.315191866729287E-4 0.0 0.0 3.4163604718623093 4.420255822305716E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 685 0.0 27.069738 2 GTACATG 4990 0.0 25.444437 1 TACATGG 5020 0.0 24.056923 2 GTACCTG 825 0.0 23.63263 1 ACATGGG 4960 0.0 23.390646 3 CATGGGG 2020 0.0 22.833815 4 GAGTACT 3310 0.0 21.690573 12-13 TAGCCCT 455 0.0 20.901464 4 GTTCCGT 140 4.815776E-4 20.378927 6 AGTACTT 3485 0.0 20.123861 12-13 ATACCGT 120 0.004733349 19.812845 6 GTATAGC 265 1.8295395E-8 19.739174 1 GTACTTT 3695 0.0 19.488396 14-15 GGACGCT 295 2.9740477E-9 19.34271 6 GTATATG 345 5.0931703E-11 19.297058 1 GGTGCGT 125 0.005985372 19.024544 8 GTCTACT 175 9.8483935E-5 19.021385 1 GTGTAGC 475 0.0 19.021383 1 CTGGACG 325 4.802132E-10 19.020332 4 TAAGGTG 430 0.0 18.800205 5 >>END_MODULE