FastQCFastQC Report
Thu 26 May 2016
SRR936448_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936448_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904925
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70470.7787385694947095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48790.5391607039257398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46050.5088819515429456No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT43170.47705610962234435No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT30900.34146476227311656No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28160.3111860098903224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26000.28731662844987155No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT23470.2593585103737879No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT20730.22907975799099373No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA16570.1831090974390143No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC14520.1604552863496975No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14260.15758212006519876No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12630.139569577589303No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG12550.13868552642484183No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12400.13702793049147718No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10060.11116943393098876No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC9690.10708069729535596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA600.003944024431.67732
GTACATG46100.023.9191931
TACATGG46850.022.9212462
ACATGGG45750.022.5313933
GAGTACT52450.022.50236912-13
GTGTAGC3900.021.93651
GTGTAAG3950.021.6588231
CGTACAC1100.002875907321.592193
GTACTTT56200.020.83070614-15
AGTACTT53850.020.7707712-13
TAAGGTG4350.020.7472115
CATGGGA27050.020.1941684
ATAAGAC2902.4338078E-919.656343
CATGGGG19250.018.7533234
CTAGGAC5100.018.6285573
GTAAGGT3851.2732926E-1118.5065694
ACTTTTT63550.018.49622216-17
TACTTTT61450.018.3168114-15
ATGGGAG11600.018.0173155
GGGCGTA1851.5221244E-417.971069
Produced by FastQC (version 0.11.5)