FastQCFastQC Report
Thu 26 May 2016
SRR936447_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936447_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086466
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45240.4163959111467823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36940.34000143584796944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30330.27916198021843297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.19816542809439042No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16430.1512242444770476No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12540.11542008677676062No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11420.1051114346882461No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11160.10271835473912667No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC550.002577614334.5507773
GTACATG55650.027.572611
TACATGG57450.026.5433392
GTACCGT908.931969E-426.3966056
ACATGGG56900.025.381773
ATGGGAG14350.024.1697125
GAGTACT43100.023.81151612-13
GTACACG1801.9487925E-723.7525671
GTACGTG1201.7094784E-423.7525671
AGTACTT45000.021.53913312-13
GTACTTT48000.021.38075814-15
CATGGGA33450.021.3047124
ATGGGCG1353.7894511E-421.1163125
GTATCAA102550.020.7994211
CATGGGG22450.020.5274934
GTACCTG8400.020.3593431
GTAAGGT3603.6379788E-1219.795634
ACGGTAG600.004778375419.78196356-57
TACCTGG8300.019.4599342
GGTTGCG1250.006019157419.0064327
Produced by FastQC (version 0.11.5)