##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936447_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1086466 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49398600600479 31.0 31.0 34.0 30.0 34.0 2 31.565445214116227 33.0 31.0 34.0 30.0 34.0 3 31.578996489535797 33.0 31.0 34.0 28.0 34.0 4 35.20114665346178 37.0 35.0 37.0 33.0 37.0 5 35.214974053490856 37.0 35.0 37.0 33.0 37.0 6 35.20367687530029 37.0 35.0 37.0 32.0 37.0 7 35.1075431720827 37.0 35.0 37.0 32.0 37.0 8 35.07994267653107 37.0 35.0 37.0 32.0 37.0 9 36.59452113549803 39.0 37.0 39.0 32.0 39.0 10-11 36.464598063814236 39.0 37.0 39.0 32.0 39.0 12-13 36.377505140519816 39.0 37.0 39.0 32.0 39.0 14-15 37.50242069241007 40.0 37.0 41.0 32.0 41.0 16-17 37.411069927636944 40.0 37.0 41.0 32.0 41.0 18-19 37.39725771446138 40.0 37.0 41.0 32.0 41.0 20-21 37.27874365143502 40.0 37.0 41.0 31.5 41.0 22-23 37.3848284253718 40.0 37.0 41.0 32.0 41.0 24-25 37.43897738171282 40.0 37.0 41.0 32.0 41.0 26-27 37.3438160973284 40.0 37.0 41.0 31.5 41.0 28-29 37.21003740568044 39.5 36.5 41.0 31.0 41.0 30-31 37.001884550459934 39.0 36.0 41.0 31.0 41.0 32-33 36.81656121774635 39.0 36.0 41.0 30.0 41.0 34-35 36.629963569959855 39.0 35.0 41.0 30.0 41.0 36-37 36.42912341481464 39.0 35.0 40.5 30.0 41.0 38-39 36.23968398458857 39.0 35.0 40.0 30.0 41.0 40-41 36.03486671465099 39.0 35.0 40.0 29.5 41.0 42-43 35.78744065621934 38.0 35.0 40.0 28.5 41.0 44-45 35.511842524294366 38.0 35.0 40.0 27.5 41.0 46-47 35.284765929168515 38.0 35.0 40.0 26.5 41.0 48-49 35.10120012959448 38.0 34.5 40.0 26.0 41.0 50-51 34.12505269377965 37.5 33.5 39.5 24.0 40.5 52-53 34.21086439888593 38.0 33.5 39.5 24.0 40.5 54-55 34.6440394821375 38.0 34.0 40.0 24.0 41.0 56-57 34.536068777117734 38.0 34.0 40.0 24.0 41.0 58-59 34.31624459486076 38.0 34.0 40.0 23.0 41.0 60-61 34.02656686909668 38.0 33.5 40.0 23.0 41.0 62-63 33.65880340480052 37.0 33.0 40.0 22.0 41.0 64-65 33.30657701207401 36.5 33.0 39.5 21.5 41.0 66-67 32.848752745138825 36.0 32.0 39.0 20.0 41.0 68-69 32.476968446320456 35.5 32.0 39.0 18.5 40.5 70-71 32.08110516113712 35.0 31.5 38.5 18.0 40.0 72-73 31.683103751060777 35.0 31.0 37.5 15.5 40.0 74-75 31.261592631522753 35.0 31.0 37.0 12.0 39.0 76-77 30.791245193130756 35.0 30.5 36.5 7.5 39.0 78-79 30.30016493843342 34.0 30.0 36.0 7.0 38.5 80-81 29.842600688838857 34.0 29.5 35.5 2.0 37.0 82-83 29.437530488758966 34.0 29.0 35.0 2.0 37.0 84-85 29.08660694398168 34.0 29.0 35.0 2.0 36.5 86-87 28.722567480252486 34.0 29.0 35.0 2.0 36.0 88-89 28.31916829426784 34.0 28.5 35.0 2.0 36.0 90-91 27.904863106622756 34.0 27.0 35.0 2.0 35.0 92-93 27.4600673191798 33.5 26.5 35.0 2.0 35.0 94-95 26.968385573041402 33.0 25.5 35.0 2.0 35.0 96-97 26.39077430863 33.0 25.0 35.0 2.0 35.0 98-99 25.65382763933708 33.0 22.0 35.0 2.0 35.0 100-101 22.767017559684334 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4871897600370616 1101 2 0.3935404534716369 1101 3 0.4746940164620881 1101 4 0.4654257418439727 1101 5 0.3532618895991604 1101 6 0.47684636301570293 1101 7 0.6451936029994201 1101 8 0.6201785716170818 1101 9 0.6213590419292814 1101 10-11 0.6947523537643647 1101 12-13 0.704706353645598 1101 14-15 0.6876920005730014 1101 16-17 0.898601462407548 1101 18-19 0.7661967074565865 1101 20-21 0.8105566653783214 1101 22-23 0.7140312996349607 1101 24-25 0.5837109042867112 1101 26-27 0.5439387118076695 1101 28-29 0.615367423950751 1101 30-31 0.46794996720578297 1101 32-33 0.5306270468446215 1101 34-35 0.37013001309865956 1101 36-37 0.34566056545861557 1101 38-39 0.4533145513252421 1101 40-41 0.41941123086794363 1101 42-43 0.34077828897024176 1101 44-45 0.46591883935911227 1101 46-47 0.4655129944553522 1101 48-49 0.5850572161914656 1101 50-51 0.45802922060173756 1101 52-53 0.569372821155433 1101 54-55 0.4100468406847142 1101 56-57 0.43515285759575306 1101 58-59 0.4834963470097122 1101 60-61 0.6273794343169925 1101 62-63 0.6257260888993628 1101 64-65 0.5183982561204843 1101 66-67 0.8285003364248595 1101 68-69 0.7995950179399287 1101 70-71 0.7480260097185862 1101 72-73 0.4277786458478623 1101 74-75 0.41208704044647604 1101 76-77 0.5737147417715569 1101 78-79 0.697389513374695 1101 80-81 0.6799228462975222 1101 82-83 0.513863022156773 1101 84-85 0.3251895142731449 1101 86-87 0.28565934441346386 1101 88-89 0.4042099180048879 1101 90-91 0.3700266228077709 1101 92-93 0.36018689098459333 1101 94-95 0.3978248778140596 1101 96-97 0.2683395350224451 1101 98-99 0.3586990865600441 1101 100-101 0.69201345869973 1102 1 0.45067516961735166 1102 2 0.4634054337309159 1102 3 0.44121187604549306 1102 4 0.3686284288997257 1102 5 0.5113866360749739 1102 6 0.5505424969836312 1102 7 0.5586449542355254 1102 8 0.543799864697057 1102 9 0.5724604439522238 1102 10-11 0.5468820116392763 1102 12-13 0.6219607044213902 1102 14-15 0.5547215006752282 1102 16-17 0.45637081033512317 1102 18-19 0.521706177644333 1102 20-21 0.4199990269609444 1102 22-23 0.5050224029175752 1102 24-25 0.5908809237595491 1102 26-27 0.40938173815669643 1102 28-29 0.17125645607055873 1102 30-31 0.5656594205556971 1102 32-33 0.36644450724017474 1102 34-35 0.492470537498761 1102 36-37 0.15198038369748446 1102 38-39 0.029986006934045406 1102 40-41 0.12449221100477104 1102 42-43 0.04466367362178403 1102 44-45 0.1324786295203495 1102 46-47 0.1395369194854439 1102 48-49 0.4256354645538778 1102 50-51 0.29880618819547067 1102 52-53 0.41819313414232084 1102 54-55 0.35248987906076223 1102 56-57 0.3787029288285382 1102 58-59 0.2705226326165402 1102 60-61 0.26772523311363017 1102 62-63 0.3540490188572605 1102 64-65 0.36957914583596363 1102 66-67 0.5245528485967483 1102 68-69 0.4381638239954313 1102 70-71 0.6163462281293377 1102 72-73 0.24225059937544202 1102 74-75 0.26123946329038716 1102 76-77 0.27719202764407314 1102 78-79 0.40341953501240724 1102 80-81 0.436434112885248 1102 82-83 0.5286024129552196 1102 84-85 0.2637609444065241 1102 86-87 0.4595170778443247 1102 88-89 0.48285599222037234 1102 90-91 0.33336524803149814 1102 92-93 0.39457297156346627 1102 94-95 0.1040804061448064 1102 96-97 0.2831863490092168 1102 98-99 0.37015236649339656 1102 100-101 0.7474637777985329 1103 1 0.33833540070018486 1103 2 0.25002852807926246 1103 3 0.2774775040459758 1103 4 0.326012866240724 1103 5 0.4315527682839857 1103 6 0.3998526302666576 1103 7 0.5564935285336361 1103 8 0.47607583857406155 1103 9 0.20863328534660752 1103 10-11 0.38033430215991615 1103 12-13 0.2127428819157089 1103 14-15 0.40746015003532676 1103 16-17 0.6265637079112878 1103 18-19 0.7006636708425305 1103 20-21 0.5875204859878451 1103 22-23 0.3431853980556241 1103 24-25 0.4104432632735566 1103 26-27 0.40966723540623207 1103 28-29 0.2748214330353491 1103 30-31 0.1628338474794475 1103 32-33 0.22763251567594267 1103 34-35 0.62267597656124 1103 36-37 0.5505183998654744 1103 38-39 0.5078395774340194 1103 40-41 0.33469295975529434 1103 42-43 0.32625402925279445 1103 44-45 0.5070348767269834 1103 46-47 0.4847923120696578 1103 48-49 0.5189854179056681 1103 50-51 0.5346889439916538 1103 52-53 0.5473322069900135 1103 54-55 0.4874043233522585 1103 56-57 0.5851573758730453 1103 58-59 0.5328402245152191 1103 60-61 0.6232160419715171 1103 62-63 0.4908489345675875 1103 64-65 0.6005634145976302 1103 66-67 0.8186133162344476 1103 68-69 0.4668061656166813 1103 70-71 0.5255343113381841 1103 72-73 0.2817333395372579 1103 74-75 0.3693078194889914 1103 76-77 0.4746915475668523 1103 78-79 0.40285788714946946 1103 80-81 0.24907994165147684 1103 82-83 0.14817053038975914 1103 84-85 0.2402796453260656 1103 86-87 0.34624944286615644 1103 88-89 0.3161918746362886 1103 90-91 0.2313798525753228 1103 92-93 0.40623757347323775 1103 94-95 0.25886240010518335 1103 96-97 0.6145934256023473 1103 98-99 0.8490434136055391 1103 100-101 1.050823298706959 1104 1 0.3208757462079532 1104 2 0.3858016619605955 1104 3 0.3091613337864132 1104 4 0.3915086532735401 1104 5 0.5146632273464888 1104 6 0.5622688915478449 1104 7 0.4462567553319019 1104 8 0.41904225417081165 1104 9 0.5694298765987043 1104 10-11 0.6187746736404307 1104 12-13 0.5008274735234721 1104 14-15 0.4958932678650356 1104 16-17 0.38724675612935044 1104 18-19 0.2977830355163178 1104 20-21 0.15422833467200547 1104 22-23 0.2690519168414056 1104 24-25 0.2767888973947308 1104 26-27 0.1919038635349537 1104 28-29 0.24784370174333503 1104 30-31 0.3860225406757536 1104 32-33 0.4181780253151075 1104 34-35 0.5030313937514563 1104 36-37 0.3626900844205707 1104 38-39 0.4030225683248432 1104 40-41 0.05191347775955535 1104 42-43 0.30679377106962136 1104 44-45 0.4822771135657007 1104 46-47 0.44824297905454813 1104 48-49 0.43282579313000014 1104 50-51 0.4209718808310754 1104 52-53 0.523522683180488 1104 54-55 0.40423653704949203 1104 56-57 0.2744656700717627 1104 58-59 0.27397088148538984 1104 60-61 0.4206400014821128 1104 62-63 0.44354664919961095 1104 64-65 0.40554784264725896 1104 66-67 0.40115284278163443 1104 68-69 0.5292273406613148 1104 70-71 0.4482296977822813 1104 72-73 0.49803691418317086 1104 74-75 0.5123650227152261 1104 76-77 0.4192098934536794 1104 78-79 0.46730160232902307 1104 80-81 0.4414107853446616 1104 82-83 0.3937773918754459 1104 84-85 0.25706014751268924 1104 86-87 0.3682710002483809 1104 88-89 0.6248727397394447 1104 90-91 0.3684007313902171 1104 92-93 0.37845784578933106 1104 94-95 0.360851655342433 1104 96-97 0.3922403646787025 1104 98-99 0.48042867371911413 1104 100-101 0.5786916496245489 1105 1 0.3624441402362919 1105 2 0.15645507041606166 1105 3 0.3870013405464121 1105 4 0.44488032639782205 1105 5 0.4164320105717536 1105 6 0.39729263852510144 1105 7 0.3794669838049103 1105 8 0.40615476588275357 1105 9 0.6893974683743735 1105 10-11 0.6833090415185197 1105 12-13 0.5603900122008483 1105 14-15 0.5232445417020983 1105 16-17 0.51514647206033 1105 18-19 0.44050348724321964 1105 20-21 0.4203398696636924 1105 22-23 0.19143869309294104 1105 24-25 0.306050740438522 1105 26-27 0.18213231169298183 1105 28-29 0.15076317575586984 1105 30-31 0.2619420991882606 1105 32-33 0.041273048018261704 1105 34-35 0.24955167250195132 1105 36-37 0.21841974167377742 1105 38-39 0.348077083232873 1105 40-41 0.18623798747332643 1105 42-43 0.23033931531156782 1105 44-45 0.15227428033136192 1105 46-47 0.11528233615888439 1105 48-49 0.2477065487585648 1105 50-51 0.17217755873210194 1105 52-53 0.2784578543053513 1105 54-55 0.19283500561177647 1105 56-57 0.0024841830213873095 1105 58-59 0.28254327932119594 1105 60-61 0.21488288173819114 1105 62-63 0.4042482599181838 1105 64-65 0.43148436328637985 1105 66-67 0.4240063571105992 1105 68-69 0.4013155089256131 1105 70-71 0.10098514243852463 1105 72-73 -0.11965756622898738 1105 74-75 0.1747279898571641 1105 76-77 0.07134650008394416 1105 78-79 0.13736307605823228 1105 80-81 0.35394927632428974 1105 82-83 0.08991860126885953 1105 84-85 0.17425017552194433 1105 86-87 -0.1463198459194217 1105 88-89 0.007282952306578494 1105 90-91 0.08055592801419564 1105 92-93 0.10747451465134006 1105 94-95 0.3540450583799597 1105 96-97 0.4343406114683255 1105 98-99 0.7091930853135047 1105 100-101 0.48486778981497025 1106 1 0.37834661649626966 1106 2 0.3539615717974982 1106 3 0.3450027853723725 1106 4 0.478143520160792 1106 5 0.21498812368137266 1106 6 0.4780177613923229 1106 7 0.5606786119037537 1106 8 0.5208809230682832 1106 9 0.6574261663473919 1106 10-11 0.7448808297153491 1106 12-13 0.6233231162222737 1106 14-15 0.596682818321213 1106 16-17 0.6214142934250972 1106 18-19 0.4804867315549757 1106 20-21 0.208014511492415 1106 22-23 0.3659510643313837 1106 24-25 0.42128298916859563 1106 26-27 0.47175418182365547 1106 28-29 0.48824205506997487 1106 30-31 0.48830286163244097 1106 32-33 0.472209495128169 1106 34-35 0.40782685617394776 1106 36-37 0.23316883899293117 1106 38-39 0.1584034301105035 1106 40-41 0.27307175937460926 1106 42-43 0.3107290561081868 1106 44-45 0.12723935028871125 1106 46-47 0.13732398122135692 1106 48-49 0.18987695370558555 1106 50-51 0.08835551782487983 1106 52-53 0.005768083391430423 1106 54-55 0.19082103627777514 1106 56-57 0.29565406007701966 1106 58-59 0.5098592099024657 1106 60-61 0.6912904299812297 1106 62-63 0.6306204253527667 1106 64-65 0.38033658522613933 1106 66-67 0.49854569306751273 1106 68-69 0.6180776365089784 1106 70-71 0.24288361960270066 1106 72-73 0.18756549034929648 1106 74-75 0.34003781246017084 1106 76-77 0.4168866228471977 1106 78-79 0.24848186369229097 1106 80-81 0.4659360327570816 1106 82-83 0.3785430451215426 1106 84-85 0.6252552149729382 1106 86-87 0.49849913831560855 1106 88-89 0.46795940452771134 1106 90-91 0.45755086441569404 1106 92-93 0.46760174519364384 1106 94-95 0.4506575653139855 1106 96-97 0.6066713223374549 1106 98-99 0.5908404417984876 1106 100-101 0.3648955862809835 1107 1 0.16608346273019947 1107 2 0.20621911477916655 1107 3 0.12001564895984274 1107 4 0.22040176781443677 1107 5 0.3121291498167196 1107 6 0.21527416759184348 1107 7 0.44250046816928545 1107 8 0.5324993684555395 1107 9 0.3637700755218205 1107 10-11 0.4531396517431219 1107 12-13 0.31264487609400504 1107 14-15 0.24645486358508606 1107 16-17 0.16892580417533765 1107 18-19 0.23150119328529684 1107 20-21 0.19638742390168318 1107 22-23 0.40486325023714187 1107 24-25 0.33266151369184627 1107 26-27 0.45390304577276197 1107 28-29 0.37255120397247765 1107 30-31 0.28417235372349126 1107 32-33 0.10763714459508122 1107 34-35 0.2767372604562226 1107 36-37 0.21289489443865506 1107 38-39 0.16064475234103526 1107 40-41 0.13936723232252035 1107 42-43 0.25709254984524677 1107 44-45 0.2853158883017741 1107 46-47 0.30429035507244606 1107 48-49 0.3970049766486454 1107 50-51 0.31783593525659626 1107 52-53 0.47385840904295407 1107 54-55 0.6475565037478574 1107 56-57 0.3342072621463643 1107 58-59 0.41725168427169024 1107 60-61 0.5418381462457234 1107 62-63 0.4092271160137386 1107 64-65 0.33157361693505294 1107 66-67 0.49217938064254696 1107 68-69 0.5569955395647241 1107 70-71 0.5970919738213567 1107 72-73 0.5047237211173972 1107 74-75 0.6207086601805578 1107 76-77 0.5783668594574323 1107 78-79 0.4096499838003034 1107 80-81 0.3451100508365741 1107 82-83 0.4351039400815431 1107 84-85 0.45817915161628164 1107 86-87 0.33455986242589475 1107 88-89 0.2711179304603064 1107 90-91 -0.039607180212641424 1107 92-93 0.17109971028711968 1107 94-95 0.21507076634626188 1107 96-97 0.22044626340427342 1107 98-99 0.4548354266613366 1107 100-101 0.14850378600363356 1108 1 0.1748265926317032 1108 2 0.25365580701265245 1108 3 0.1650490229739816 1108 4 0.2701861780771182 1108 5 0.34933818707163056 1108 6 0.09819823719216458 1108 7 0.12477735155622582 1108 8 0.03855561916791572 1108 9 0.3880548485757416 1108 10-11 0.33290007052375614 1108 12-13 0.11137218774140933 1108 14-15 0.35412185180618394 1108 16-17 0.2689644463074998 1108 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-2.591746529323675 2316 74-75 -2.622243867212809 2316 76-77 -2.4390766190881514 2316 78-79 -3.2256989550332342 2316 80-81 -3.1360971086525957 2316 82-83 -3.3268111212662603 2316 84-85 -3.162115239747802 2316 86-87 -3.0360241726638115 2316 88-89 -3.3210259242443243 2316 90-91 -3.3809120126033747 2316 92-93 -4.141493243310695 2316 94-95 -4.0463670125145335 2316 96-97 -4.632977325432602 2316 98-99 -5.236533442179169 2316 100-101 -5.677573657172676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2122.0 3 908.0 4 835.0 5 959.0 6 1119.0 7 1338.0 8 1622.0 9 1962.0 10 2609.0 11 3312.0 12 4382.0 13 5297.0 14 6178.0 15 7327.0 16 9122.0 17 11826.0 18 9648.0 19 7128.0 20 7077.0 21 7594.0 22 8124.0 23 9337.0 24 10700.0 25 12246.0 26 14158.0 27 16730.0 28 20090.0 29 24208.0 30 29313.0 31 36049.0 32 45379.0 33 58251.0 34 77473.0 35 106014.0 36 149260.0 37 197874.0 38 156424.0 39 22465.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.661940545133355 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27.18642981964016 28.738189903181254 22.193202115404706 44-45 22.075356209270648 27.20532196899967 28.615846250138066 22.10347557159162 46-47 22.454600806022942 27.39622005889849 28.170718181445825 21.97846095363274 48-49 22.51576570253848 27.56405801796943 27.87819852888726 22.041977750604833 50-51 22.200412638349594 27.845264760933468 27.96381889751096 21.990503703205977 52-53 22.561738636588522 27.925069114964867 27.58003506028316 21.93315718816345 54-55 22.01483813363525 27.631605592834983 27.839541969274386 22.51401430425538 56-57 22.334549983041153 28.022184106360744 27.469560363144858 22.173705547453253 58-59 22.703875697841923 27.470141800558736 27.518283113260956 22.30769938833839 60-61 22.301334835506516 27.57228748207425 28.26276248911569 21.86361519330355 62-63 22.29506868896149 27.682081778775107 28.0945699110548 21.9282796212086 64-65 21.953555370447926 28.094766925459407 27.774643220381883 22.177034483710784 66-67 22.006068506366777 28.019247623045594 27.50210814314016 22.47257572744747 68-69 21.969437461440002 27.86389126771458 27.87052128511837 22.296149985727045 70-71 22.163821673766677 27.854390762909993 27.575931585706897 22.405855977616433 72-73 22.094435701506114 27.7823218554075 27.690448123792027 22.43279431929436 74-75 21.940988098548363 28.344256348024004 27.42839885607585 22.286356697351774 76-77 22.22940363010962 27.910956066356206 27.442139028373923 22.417501275160244 78-79 22.175312564757892 27.98950058713822 27.409269876355598 22.42591697174829 80-81 22.239045149676713 28.226574278240367 27.371430124381675 22.162950447701242 82-83 22.198687694255785 28.247910671117758 27.426407275238862 22.12699435938759 84-85 22.10867663981588 28.245247410817033 27.106789413118527 22.53928653624856 86-87 22.247411234432423 28.14228452117043 27.1325554761782 22.477748768218945 88-89 22.171137335845433 28.084453321389656 27.012146367712674 22.732262975052233 90-91 22.09225876136867 27.92063271359102 27.42863877995081 22.5584697450895 92-93 22.293870313476766 27.972891979454467 27.391999248738465 22.341238458330302 94-95 21.90088065274996 27.907481981208633 27.43551139122698 22.756125974814427 96-97 21.849152607127223 27.74493011925963 27.60955531609625 22.796361957516893 98-99 21.86534204138165 28.04979684904208 27.05678833622496 23.02807277335131 100-101 21.78714295319879 27.93998583372403 27.204960591409783 23.0679106216674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 291.0 1 220.5 2 213.5 3 297.5 4 281.5 5 289.0 6 446.0 7 766.0 8 1195.0 9 1461.0 10 1365.0 11 1209.5 12 1247.5 13 1358.0 14 1480.0 15 1546.5 16 1599.0 17 1730.0 18 1850.0 19 1982.0 20 2171.0 21 2514.0 22 2964.5 23 3476.5 24 4211.5 25 5132.5 26 6334.0 27 7825.5 28 9403.5 29 11125.5 30 13195.0 31 15814.0 32 18680.5 33 20360.5 34 21922.5 35 23731.5 36 25668.0 37 28073.0 38 29404.5 39 29818.5 40 30783.0 41 32951.0 42 34793.0 43 36397.5 44 37943.5 45 39571.0 46 40335.0 47 42195.5 48 49348.0 49 48931.5 50 41278.5 51 37470.5 52 35012.5 53 34115.5 54 33384.0 55 31060.0 56 28178.0 57 25350.5 58 23062.5 59 20427.5 60 17052.5 61 14174.5 62 11923.5 63 9402.5 64 6964.5 65 5457.0 66 4190.0 67 3292.5 68 2376.5 69 1528.0 70 1148.0 71 868.5 72 713.5 73 623.0 74 484.5 75 385.0 76 296.5 77 175.5 78 105.5 79 61.0 80 33.5 81 27.5 82 22.5 83 17.0 84 9.0 85 6.0 86 6.5 87 5.0 88 6.0 89 3.5 90 0.5 91 2.0 92 2.0 93 1.0 94 0.5 95 0.5 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.015002770450248788 2 0.014358479694716632 3 0.017119725789854445 4 0.021445678005570352 5 0.03350311928767214 6 0.054028381928196555 7 0.0069031152378445345 8 0.027060211732350577 9 0.03746090535736967 10-11 0.043075439084149895 12-13 0.03483772156698875 14-15 0.029913499363992985 16-17 0.032858828532139985 18-19 0.025863671757790856 20-21 0.06005710256924744 22-23 0.015647061205780945 24-25 0.0029453291681470015 26-27 0.0018408307300918757 28-29 9.20415365045938E-5 30-31 0.0031294122411561886 32-33 0.002485121485624032 34-35 0.0 36-37 5.982699872798597E-4 38-39 1.380623047568907E-4 40-41 0.0021629761078579544 42-43 0.005016263739500362 44-45 0.0023930799491194386 46-47 0.0018868514983441727 48-49 8.74394596793641E-4 50-51 0.003911765301445236 52-53 0.0047401391299865805 54-55 0.006995156774349128 56-57 0.0011505192063074226 58-59 0.007455364456872098 60-61 0.0036816614601837515 62-63 9.664361332982349E-4 64-65 0.0010124569015505318 66-67 0.018592390373927944 68-69 0.04565260210627852 70-71 0.06162180868982555 72-73 0.017717995777134305 74-75 0.03497578387174564 76-77 0.030189623973506764 78-79 0.06360070172467432 80-81 0.08987856039673583 82-83 0.05439654807421493 84-85 0.019880971884992257 86-87 0.021537719542074948 88-89 0.023608654113428308 90-91 0.04390381291269124 92-93 0.02696817019584598 94-95 0.015601040437528647 96-97 0.020479241872272118 98-99 0.05283184195363684 100-101 0.0726207723021245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1086466.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.2817115157192 #Duplication Level Percentage of deduplicated Percentage of total 1 83.91707468840735 54.782502610516715 2 9.992646981966319 13.046741951102947 3 2.7341751466432496 5.354748994698416 4 1.0724304273831295 2.800403751244197 5 0.5630451049643637 1.8378274056310717 6 0.34283063377080997 1.3428342319546318 7 0.2347638755673791 1.0728051319371288 8 0.1679384287008596 0.8770646443882155 9 0.1272370613969375 0.7475627822600452 >10 0.7547351801834273 9.13638916431893 >50 0.06044663529975908 2.6732844585580975 >100 0.029280945692917802 3.6191250516925106 >500 0.0019803525137062597 0.8018179995839755 >1k 0.0014145375097901853 1.9068918221132207 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4524 0.4163959111467823 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3694 0.34000143584796944 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3033 0.27916198021843297 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2153 0.19816542809439042 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1643 0.1512242444770476 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1254 0.11542008677676062 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1142 0.1051114346882461 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1116 0.10271835473912667 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.20415365045938E-5 2 0.0 0.0 0.0 0.0 9.20415365045938E-5 3 0.0 0.0 0.0 0.0 9.20415365045938E-5 4 0.0 0.0 0.0 0.0 9.20415365045938E-5 5 0.0 0.0 0.0 0.0 9.20415365045938E-5 6 0.0 0.0 0.0 0.0 9.20415365045938E-5 7 0.0 0.0 0.0 0.0 9.20415365045938E-5 8 0.0 0.0 0.0 0.0 9.20415365045938E-5 9 0.0 0.0 0.0 0.0 9.20415365045938E-5 10-11 0.0 0.0 0.0 0.0 9.20415365045938E-5 12-13 0.0 0.0 0.0 0.0 9.20415365045938E-5 14-15 0.0 0.0 0.0 0.0 9.20415365045938E-5 16-17 0.0 0.0 0.0 9.20415365045938E-5 9.20415365045938E-5 18-19 0.0 0.0 0.0 9.20415365045938E-5 9.20415365045938E-5 20-21 0.0 0.0 0.0 9.20415365045938E-5 9.20415365045938E-5 22-23 0.0 0.0 0.0 1.380623047568907E-4 9.20415365045938E-5 24-25 0.0 0.0 0.0 1.840830730091876E-4 9.20415365045938E-5 26-27 0.0 0.0 0.0 4.1418691427067206E-4 1.840830730091876E-4 28-29 0.0 0.0 0.0 0.0011505192063074223 2.761246095137814E-4 30-31 0.0 0.0 0.0 0.002208996876110251 2.761246095137814E-4 32-33 0.0 0.0 0.0 0.003911765301445236 2.761246095137814E-4 34-35 0.0 0.0 0.0 0.005246367580761847 2.761246095137814E-4 36-37 0.0 0.0 0.0 0.007731489066385879 2.761246095137814E-4 38-39 0.0 0.0 0.0 0.010860901307542067 2.761246095137814E-4 40-41 0.0 0.0 0.0 0.013990313548698258 2.761246095137814E-4 42-43 0.0 0.0 0.0 0.0222740518341117 2.761246095137814E-4 44-45 0.0 0.0 0.0 0.030741873192534328 2.761246095137814E-4 46-47 0.0 0.0 0.0 0.03939377762396615 2.761246095137814E-4 48-49 0.0 0.0 0.0 0.05020865816325591 2.761246095137814E-4 50-51 0.0 0.0 0.0 0.06691419703883969 2.761246095137814E-4 52-53 0.0 0.0 0.0 0.09899067251069063 2.761246095137814E-4 54-55 0.0 0.0 0.0 0.12361178352566946 2.761246095137814E-4 56-57 0.0 0.0 0.0 0.1701848009969939 2.761246095137814E-4 58-59 0.0 0.0 0.0 0.225225639826741 2.761246095137814E-4 60-61 0.0 0.0 0.0 0.2819232263135708 2.761246095137814E-4 62-63 0.0 0.0 0.0 0.36209140460907197 2.761246095137814E-4 64-65 0.0 0.0 0.0 0.4042464283281759 2.761246095137814E-4 66-67 0.0 0.0 0.0 0.5059983469340044 2.761246095137814E-4 68-69 0.0 0.0 0.0 0.6386302010371241 2.761246095137814E-4 70-71 0.0 0.0 0.0 0.7246890376689192 2.761246095137814E-4 72-73 0.0 0.0 0.0 0.8846572281139032 2.761246095137814E-4 74-75 0.0 0.0 0.0 1.0408517155621988 2.761246095137814E-4 76-77 0.0 0.0 0.0 1.1340897920413524 2.761246095137814E-4 78-79 9.20415365045938E-5 0.0 0.0 1.2263154116189554 2.761246095137814E-4 80-81 9.20415365045938E-5 0.0 0.0 1.4181759944627812 2.761246095137814E-4 82-83 9.20415365045938E-5 0.0 0.0 1.5760732503364119 2.761246095137814E-4 84-85 9.20415365045938E-5 0.0 0.0 1.827990935749485 2.761246095137814E-4 86-87 9.20415365045938E-5 0.0 0.0 2.200621096288333 2.761246095137814E-4 88-89 9.20415365045938E-5 0.0 0.0 2.680295563782024 2.761246095137814E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 55 0.0025776143 34.550777 3 GTACATG 5565 0.0 27.57261 1 TACATGG 5745 0.0 26.543339 2 GTACCGT 90 8.931969E-4 26.396605 6 ACATGGG 5690 0.0 25.38177 3 ATGGGAG 1435 0.0 24.169712 5 GAGTACT 4310 0.0 23.811516 12-13 GTACACG 180 1.9487925E-7 23.752567 1 GTACGTG 120 1.7094784E-4 23.752567 1 AGTACTT 4500 0.0 21.539133 12-13 GTACTTT 4800 0.0 21.380758 14-15 CATGGGA 3345 0.0 21.304712 4 ATGGGCG 135 3.7894511E-4 21.116312 5 GTATCAA 10255 0.0 20.799421 1 CATGGGG 2245 0.0 20.527493 4 GTACCTG 840 0.0 20.359343 1 GTAAGGT 360 3.6379788E-12 19.79563 4 ACGGTAG 60 0.0047783754 19.781963 56-57 TACCTGG 830 0.0 19.459934 2 GGTTGCG 125 0.0060191574 19.006432 7 >>END_MODULE