FastQCFastQC Report
Thu 26 May 2016
SRR936447_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936447_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1086466
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67650.622660994453577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57340.5277661703173407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34640.3188318824519129No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31050.2857889708467637No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18550.17073705021602148No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16530.15214465984209355No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13490.12416403274469702No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12060.1110020930245401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG51850.025.2026581
TACATGG53100.023.8890632
GAGTACT60750.023.81077212-13
GTAAGGT4450.023.4836254
ACATGGG51350.023.2185823
GTATCAA105400.023.2182251
GGTATCA81350.022.0214631
GTACTTT65850.021.78680814-15
TAAGGTG5300.021.5098725
CCACGTC3151.4551915E-1121.1086259
AGTACTT62150.020.94314212-13
TCAACGC123500.019.8465584
ATCAACG123200.019.8177743
TATCAAC123050.019.8069742
ATATAGG3603.6379788E-1219.7993621
CAACGCA124200.019.7347015
CTGTTCG1707.960538E-519.5565229
AACGCAG126700.019.3810086
AGACCCG3707.2759576E-1219.2571495
CATGGGA31150.019.2138754
Produced by FastQC (version 0.11.5)