FastQCFastQC Report
Thu 26 May 2016
SRR936446_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936446_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173876
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49500.4216799730124818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40290.34322194167015935No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30160.2569266259809384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23280.19831736912587022No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17020.14498976041762504No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12900.1098923566032528No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12080.10290695098971273No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11790.10043650266297291No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA700.00837877627.1513969
GTACATG59750.026.6413141
TACATGG60650.025.77482
ACATGGG59300.025.0795543
GAGTACT46350.023.88357512-13
CGTACAC1000.001650641623.7574713
TACCTGG9900.022.5575982
GTACTTT49700.022.3196114-15
GTGTAAG3950.021.65331
GTATCAA106350.021.4909841
CATGGGG25100.021.202794
AGTACTT47700.020.46852912-13
GTACCTG10950.020.3954091
GTAAGGT3750.020.2739054
CATGGGA34850.019.9066774
GTATAGG4100.019.7021561
ACTTTTT59850.019.1297416-17
TATCAAC119350.018.9900532
TCAACGC119100.018.9110344
ATCAACG119600.018.910633
Produced by FastQC (version 0.11.5)