##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936446_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173876 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.335474104590265 31.0 31.0 34.0 30.0 34.0 2 31.36862496549891 31.0 31.0 34.0 28.0 34.0 3 31.4155183341341 31.0 31.0 34.0 28.0 34.0 4 35.060941700827 37.0 35.0 37.0 32.0 37.0 5 34.97430648552317 37.0 35.0 37.0 32.0 37.0 6 35.10644650712682 37.0 35.0 37.0 32.0 37.0 7 34.92523912236045 37.0 35.0 37.0 32.0 37.0 8 34.919535794240616 37.0 35.0 37.0 32.0 37.0 9 36.41535306965983 39.0 37.0 39.0 32.0 39.0 10-11 36.289157457857556 39.0 35.0 39.0 32.0 39.0 12-13 36.19093158050765 39.0 35.0 39.0 31.5 39.0 14-15 37.256221696329085 39.5 37.0 41.0 32.0 41.0 16-17 37.209100876072085 39.0 37.0 41.0 31.0 41.0 18-19 37.19392380455857 39.5 36.5 41.0 31.0 41.0 20-21 37.120728254091574 39.0 36.0 41.0 31.0 41.0 22-23 37.033311014110524 39.0 36.0 41.0 31.0 41.0 24-25 36.87739037172581 39.0 36.0 41.0 30.5 41.0 26-27 36.665190360821754 39.0 36.0 40.5 30.0 41.0 28-29 36.77050131359701 39.0 36.0 41.0 30.0 41.0 30-31 36.71683891654655 39.0 36.0 41.0 30.0 41.0 32-33 36.56952608282306 39.0 35.0 41.0 30.0 41.0 34-35 36.404150438376796 39.0 35.0 40.5 30.0 41.0 36-37 36.159998585881304 39.0 35.0 40.0 29.5 41.0 38-39 35.98552487656278 38.0 35.0 40.0 29.0 41.0 40-41 35.83888971237167 38.0 35.0 40.0 28.5 41.0 42-43 35.5744252374186 38.0 35.0 40.0 27.5 41.0 44-45 35.29095023665191 38.0 34.5 40.0 26.5 41.0 46-47 34.932247954639166 38.0 34.0 40.0 25.5 41.0 48-49 34.65578306396928 38.0 34.0 40.0 24.0 41.0 50-51 33.936869822706996 37.5 33.0 39.5 23.5 40.5 52-53 34.113631678303335 38.0 33.5 39.5 24.0 40.5 54-55 34.46385776691916 38.0 34.0 40.0 23.5 41.0 56-57 34.39261344469092 38.0 34.0 40.0 23.0 41.0 58-59 34.16569424709253 38.0 34.0 40.0 23.0 41.0 60-61 33.8514063666009 37.5 33.0 40.0 22.5 41.0 62-63 33.53212818048925 37.0 33.0 40.0 22.0 41.0 64-65 33.229039097826345 36.5 32.5 39.5 21.0 41.0 66-67 32.87201373910021 36.0 32.0 39.0 20.0 41.0 68-69 32.50186817006225 35.5 32.0 39.0 19.5 40.5 70-71 32.10233874787456 35.0 31.0 38.5 18.0 40.0 72-73 31.691821367844646 35.0 31.0 37.5 16.0 40.0 74-75 31.281019034378417 35.0 31.0 37.0 13.0 39.0 76-77 30.850812181184384 35.0 30.5 36.5 9.0 39.0 78-79 30.407182274788816 34.5 30.0 36.0 7.0 38.5 80-81 29.99492578432475 34.0 30.0 35.5 6.0 37.5 82-83 29.587450463251656 34.0 29.0 35.0 2.0 37.0 84-85 29.212559503729523 34.0 29.0 35.0 2.0 36.5 86-87 28.875049408966532 34.0 29.0 35.0 2.0 36.0 88-89 28.501175592652032 34.0 29.0 35.0 2.0 36.0 90-91 28.159290248714512 34.0 28.0 35.0 2.0 35.0 92-93 27.803319515860277 34.0 27.0 35.0 2.0 35.0 94-95 27.386597051136576 34.0 26.5 35.0 2.0 35.0 96-97 26.819979282309205 33.0 25.5 35.0 2.0 35.0 98-99 26.166635999032266 33.0 24.5 35.0 2.0 35.0 100-101 24.095505402614926 31.0 11.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3512103969473337 1101 2 0.382073586610602 1101 3 0.3959141140850768 1101 4 0.42774876768424264 1101 5 0.49031126917498824 1101 6 0.333806988617809 1101 7 0.4199335146007144 1101 8 0.43341100600405014 1101 9 0.6718069983517552 1101 10-11 0.4955394462009721 1101 12-13 0.5298466894253266 1101 14-15 0.7369148772653844 1101 16-17 0.7905836474850787 1101 18-19 0.8013421657989355 1101 20-21 0.6279162825086146 1101 22-23 0.5589607759156152 1101 24-25 0.6282130235645695 1101 26-27 0.6985031426479793 1101 28-29 0.4856286130506646 1101 30-31 0.4396718293421529 1101 32-33 0.4047022140198422 1101 34-35 0.40456771155614746 1101 36-37 0.45607605343098356 1101 38-39 0.6055584143139257 1101 40-41 0.5529298613305329 1101 42-43 0.446515000733541 1101 44-45 0.4485964593742082 1101 46-47 0.45185134947990235 1101 48-49 0.3908648256653109 1101 50-51 0.694338047922713 1101 52-53 0.613015305438303 1101 54-55 0.6081534414507317 1101 56-57 0.5688263573684864 1101 58-59 0.46281668264557396 1101 60-61 0.30021748701481954 1101 62-63 0.4855757267689498 1101 64-65 0.4602447138648742 1101 66-67 0.5083516254548002 1101 68-69 0.3773271690146558 1101 70-71 0.4283813234427498 1101 72-73 0.35737902714224745 1101 74-75 0.3625759721548967 1101 76-77 0.3287717536930117 1101 78-79 0.5310180391060122 1101 80-81 0.4099913558046744 1101 82-83 0.43704787482399965 1101 84-85 0.4496293029623608 1101 86-87 0.5325770587028984 1101 88-89 0.4915590292268952 1101 90-91 0.48227563755121494 1101 92-93 0.3155022625669197 1101 94-95 0.5098790894985363 1101 96-97 0.3851772021596709 1101 98-99 0.3192210319398576 1101 100-101 0.700874570264876 1102 1 0.031002005502543284 1102 2 0.27399358175173205 1102 3 0.30181516414041454 1102 4 0.3369706064957967 1102 5 0.22848183793247046 1102 6 0.22315358336123836 1102 7 0.04216947112284686 1102 8 0.16483370951413434 1102 9 0.43037637668660267 1102 10-11 0.47071451166071654 1102 12-13 0.42511193637278666 1102 14-15 0.4507154679777514 1102 16-17 0.3138907481806186 1102 18-19 0.3074961694160905 1102 20-21 0.1374639016633452 1102 22-23 -0.10904498499210291 1102 24-25 -0.03262534484559154 1102 26-27 -0.024349901342773705 1102 28-29 0.06483603272187821 1102 30-31 0.11505587111042104 1102 32-33 -0.11308945302173612 1102 34-35 -0.09498801695910686 1102 36-37 -0.17663936762591703 1102 38-39 -0.12236438592361765 1102 40-41 -0.46948588455067153 1102 42-43 -0.2786007348000723 1102 44-45 -0.3330126132118849 1102 46-47 -0.15207837239489663 1102 48-49 -0.01089325220254267 1102 50-51 -0.0908715919811911 1102 52-53 -0.15327455527415879 1102 54-55 -0.28387958985297246 1102 56-57 -0.05438699642007805 1102 58-59 -0.14768797601895756 1102 60-61 -0.4919292552942167 1102 62-63 -0.43983193449597735 1102 64-65 -0.03267787267245836 1102 66-67 -0.16200624366169336 1102 68-69 -0.17975496834734628 1102 70-71 -0.13014553979811438 1102 72-73 -0.2093845731888777 1102 74-75 -0.17097608521573449 1102 76-77 -0.1434832109002393 1102 78-79 -0.059507337231302415 1102 80-81 -0.05091472182923695 1102 82-83 0.0445323309457244 1102 84-85 -0.0014980218709546023 1102 86-87 -0.07417828605177235 1102 88-89 -0.18487917317194658 1102 90-91 -0.23038193823362718 1102 92-93 -0.21802195753979703 1102 94-95 -0.23747383976894554 1102 96-97 -0.4273857831647483 1102 98-99 -0.6272245712346027 1102 100-101 0.11478516034738462 1103 1 0.1044041183527682 1103 2 0.2887822083990841 1103 3 0.2708864640026185 1103 4 0.2610488844168657 1103 5 0.24322629965030984 1103 6 0.07832132176352502 1103 7 0.10186941459173937 1103 8 -0.06207244884363661 1103 9 0.2758123728707531 1103 10-11 0.3481579363860092 1103 12-13 0.13386520457756745 1103 14-15 0.30181892874163196 1103 16-17 0.44159032949708177 1103 18-19 0.19071747104489845 1103 20-21 0.027677615757262686 1103 22-23 0.054081494533043895 1103 24-25 0.11543868546922198 1103 26-27 0.17625714561337702 1103 28-29 0.32232640476724583 1103 30-31 0.14066821785811356 1103 32-33 0.23226042154986004 1103 34-35 0.11852733477039123 1103 36-37 0.3164234260708625 1103 38-39 0.22579548228822688 1103 40-41 0.18764078930015415 1103 42-43 0.25865459149041925 1103 44-45 0.3588817972746412 1103 46-47 0.35567478098106875 1103 48-49 0.37862596837478435 1103 50-51 -0.24294800614223533 1103 52-53 0.11996834300517634 1103 54-55 0.3749154142102 1103 56-57 0.2948449754556961 1103 58-59 0.38035565009841577 1103 60-61 0.6036546934037972 1103 62-63 0.4076654597731775 1103 64-65 0.5717169794633534 1103 66-67 0.3699139591436662 1103 68-69 0.3288152362246066 1103 70-71 0.3684367659645318 1103 72-73 0.2214520430708795 1103 74-75 0.3223152619912746 1103 76-77 0.2126936961414181 1103 78-79 0.3880523295884686 1103 80-81 0.5000394614728876 1103 82-83 0.431417113477611 1103 84-85 0.0640272758006688 1103 86-87 0.004436392604198147 1103 88-89 0.1722274056359545 1103 90-91 0.3479268982854684 1103 92-93 0.13478406125679854 1103 94-95 -0.15170333840866235 1103 96-97 -0.19886098014033493 1103 98-99 -0.056079286282479046 1103 100-101 -0.05058048110404911 1104 1 0.1940443815868349 1104 2 0.21423830202532912 1104 3 0.3540535745532978 1104 4 0.25624506469585384 1104 5 0.35442036633836693 1104 6 0.23487625829970682 1104 7 0.2681312754941203 1104 8 0.32139453583395294 1104 9 0.32369294825213046 1104 10-11 0.45787787436939453 1104 12-13 0.28154676136517764 1104 14-15 0.2998396809735766 1104 16-17 0.24032688676710023 1104 18-19 0.4761714443490277 1104 20-21 0.445627600836481 1104 22-23 0.4120891882732636 1104 24-25 0.5659964760262639 1104 26-27 0.4768043906530579 1104 28-29 0.5030521719536338 1104 30-31 0.568253608502225 1104 32-33 0.390646800331389 1104 34-35 0.2262537212254614 1104 36-37 0.3689316885959144 1104 38-39 0.43290018805708286 1104 40-41 0.2929952757446799 1104 42-43 0.3073330923217199 1104 44-45 0.0704745181751818 1104 46-47 0.3183674890923669 1104 48-49 0.13670114220753504 1104 50-51 -0.036674601263918305 1104 52-53 0.24410983692355614 1104 54-55 0.5553722146104789 1104 56-57 0.7246255726012194 1104 58-59 0.5045843689078069 1104 60-61 0.5778784760135665 1104 62-63 0.5070945434125989 1104 64-65 0.5126382425919616 1104 66-67 0.4535399118862813 1104 68-69 0.6077464143832714 1104 70-71 0.7109049667422056 1104 72-73 0.45641318171751166 1104 74-75 0.10331964399030014 1104 76-77 0.4235100874653739 1104 78-79 0.5427752629475293 1104 80-81 0.6638576230975239 1104 82-83 0.5136743926892571 1104 84-85 0.42246043629222285 1104 86-87 0.39846179608242593 1104 88-89 0.5527736185611722 1104 90-91 0.445230611429448 1104 92-93 0.27017672471792764 1104 94-95 0.11438558722748837 1104 96-97 0.45976323371378314 1104 98-99 0.42197874547523284 1104 100-101 0.18223677629856638 1105 1 0.11803036974320236 1105 2 0.07761323276959686 1105 3 0.09634909925391 1105 4 0.1880890300332041 1105 5 0.37142429742561234 1105 6 0.19504192040848523 1105 7 0.1932841177973188 1105 8 -0.3347744409560818 1105 9 0.08552518508634677 1105 10-11 0.1555954932673913 1105 12-13 0.08092759713495923 1105 14-15 0.27997647753321786 1105 16-17 0.2730191898469698 1105 18-19 0.021552236946398295 1105 20-21 -0.19597998359554936 1105 22-23 -0.4163907473518833 1105 24-25 -0.3205887187172536 1105 26-27 -0.24149237405494262 1105 28-29 -0.0839204351558962 1105 30-31 -0.19830690601690293 1105 32-33 -0.35488185775037095 1105 34-35 -0.357923772520536 1105 36-37 -0.5407537404778253 1105 38-39 -0.5280218905502707 1105 40-41 -0.6252826609981526 1105 42-43 -0.4801081752200673 1105 44-45 -0.2917527461574565 1105 46-47 -0.564868246625359 1105 48-49 -0.30542954785537546 1105 50-51 -0.31195144430585486 1105 52-53 -0.28634169845502555 1105 54-55 -0.22146692980840754 1105 56-57 0.02280027932868478 1105 58-59 -0.03951744121516754 1105 60-61 0.10360413217415498 1105 62-63 -0.3273557759432677 1105 64-65 -0.33384728385953366 1105 66-67 -0.47966638265024386 1105 68-69 -0.19329266143946455 1105 70-71 -0.16374545670712592 1105 72-73 -0.2266342143869018 1105 74-75 -0.23019257499932166 1105 76-77 -0.043537975412832 1105 78-79 -0.22231019442811473 1105 80-81 -0.09536957887497621 1105 82-83 -0.12076207552026474 1105 84-85 -0.4326104024276667 1105 86-87 -0.21903160213743078 1105 88-89 -0.06529661094812766 1105 90-91 -0.22542480562796996 1105 92-93 -0.31550160969071683 1105 94-95 -0.3422180517264941 1105 96-97 -0.3188174974828364 1105 98-99 -0.37235241804504327 1105 100-101 0.012317688579440045 1106 1 0.09911837341320862 1106 2 0.11372200765044838 1106 3 0.099608777618716 1106 4 0.27487955938050845 1106 5 0.4329325676807301 1106 6 0.22247648472278314 1106 7 0.3930228847774657 1106 8 0.33251663634339934 1106 9 0.36991746788064717 1106 10-11 0.39269695362369106 1106 12-13 0.4207879330423907 1106 14-15 0.37694171442696245 1106 16-17 0.204386266840622 1106 18-19 0.40598402327054117 1106 20-21 0.3840165757744316 1106 22-23 0.3815276714874827 1106 24-25 0.24449716960244672 1106 26-27 0.22785097533466825 1106 28-29 0.08773004780660898 1106 30-31 0.10587218514705654 1106 32-33 0.1532698867764779 1106 34-35 0.2444649167268622 1106 36-37 0.3815880542567385 1106 38-39 0.13000178608147195 1106 40-41 0.004871912491154262 1106 42-43 0.15213562527436864 1106 44-45 0.08352059278282553 1106 46-47 0.2456772843785373 1106 48-49 0.1336005767718902 1106 50-51 0.28357191374905 1106 52-53 0.12351124646940548 1106 54-55 0.47211756951621453 1106 56-57 0.35357289191374264 1106 58-59 0.5720354297994348 1106 60-61 0.6710360204428838 1106 62-63 0.4884500960652858 1106 64-65 0.3917650208528656 1106 66-67 0.3548673482669997 1106 68-69 0.3659632704615561 1106 70-71 0.5395135845039789 1106 72-73 0.6970564347452957 1106 74-75 0.29895975570893896 1106 76-77 0.18465862473498618 1106 78-79 0.43983331807284287 1106 80-81 0.3634225051273674 1106 82-83 0.5198335357649988 1106 84-85 0.482808717280772 1106 86-87 0.4942141862765652 1106 88-89 0.7206955648043483 1106 90-91 0.9820458215861372 1106 92-93 0.8184440283549996 1106 94-95 0.6580068635320622 1106 96-97 0.6294035693673301 1106 98-99 0.864461685989415 1106 100-101 0.9779910326169521 1107 1 0.2665999793456564 1107 2 0.2831173690624951 1107 3 0.31906110753394046 1107 4 0.060869169870436224 1107 5 0.20056673333355235 1107 6 0.25693180789834 1107 7 0.38146310647184123 1107 8 0.32130639359964164 1107 9 0.433513628782876 1107 10-11 0.38046127782275363 1107 12-13 0.47887359822208 1107 14-15 0.5252770754527845 1107 16-17 0.5078217572525361 1107 18-19 0.33969682476909924 1107 20-21 0.3519587575715448 1107 22-23 0.5004431456778491 1107 24-25 0.5680859921890047 1107 26-27 0.5828613069106723 1107 28-29 0.3365964711501803 1107 30-31 0.3396689910486046 1107 32-33 0.1312423874040931 1107 34-35 0.11799097740314579 1107 36-37 0.22893274266798613 1107 38-39 0.6406809665074817 1107 40-41 0.6476987089301431 1107 42-43 0.7109840890559198 1107 44-45 0.4849283907087951 1107 46-47 0.9007523090005165 1107 48-49 0.7033503015850542 1107 50-51 0.5995501305383399 1107 52-53 0.6130618711918032 1107 54-55 0.8461071027607119 1107 56-57 0.8125874333655716 1107 58-59 0.8981607991291369 1107 60-61 0.9049438666472298 1107 62-63 1.0048628183872879 1107 64-65 0.6976347078157588 1107 66-67 0.5223421952244962 1107 68-69 0.7248629093391727 1107 70-71 0.6469986664806839 1107 72-73 0.7152882868975432 1107 74-75 0.8439088702832152 1107 76-77 0.6868118410414965 1107 78-79 0.813873060845566 1107 80-81 0.9570777587820416 1107 82-83 0.9286221343398218 1107 84-85 1.111824031186881 1107 86-87 0.8012840740775786 1107 88-89 0.7405833658201288 1107 90-91 0.7833010597917287 1107 92-93 0.6426430898230535 1107 94-95 0.8698563121928196 1107 96-97 1.104020721714626 1107 98-99 1.0722180925321005 1107 100-101 1.63086939037915 1108 1 -0.017886776717972452 1108 2 0.08221551330263921 1108 3 -0.05706857799945908 1108 4 0.015272459325444743 1108 5 0.23644696537451182 1108 6 0.15737753094439455 1108 7 0.24249167627812795 1108 8 0.19820024337867892 1108 9 0.36805337951864914 1108 10-11 0.11912538623470681 1108 12-13 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0.6896240434561349 2102 16-17 0.6789199827660184 2102 18-19 0.6099235259008609 2102 20-21 0.5208799186725628 2102 22-23 0.5338501248590717 2102 24-25 0.3302157253174087 2102 26-27 0.615276250322708 2102 28-29 0.6704924820485942 2102 30-31 0.5039024338318896 2102 32-33 0.513704487446013 2102 34-35 0.5072488717722905 2102 36-37 0.3183198811338315 2102 38-39 0.30186931358027635 2102 40-41 0.4536404442941162 2102 42-43 0.3402334253700161 2102 44-45 0.44288214228067346 2102 46-47 0.3275460074775296 2102 48-49 0.25053924248208403 2102 50-51 0.5077021146098772 2102 52-53 0.47627009391082 2102 54-55 0.2981278516778545 2102 56-57 0.2848139249123065 2102 58-59 0.4879339233431992 2102 60-61 0.25803087936205316 2102 62-63 0.28074744183286526 2102 64-65 0.24989211315319437 2102 66-67 0.2957807868131468 2102 68-69 0.2644867042252983 2102 70-71 0.45670332625432764 2102 72-73 0.38193807032635974 2102 74-75 0.3554369197452836 2102 76-77 0.5074646983839557 2102 78-79 0.4449265748877629 2102 80-81 0.41154541282703505 2102 82-83 0.3095394181179394 2102 84-85 0.20287387749119645 2102 86-87 0.3436193751813974 2102 88-89 0.3938991755169283 2102 90-91 0.38816430556509474 2102 92-93 0.3002230814396505 2102 94-95 0.33592711699930433 2102 96-97 0.3204216229302226 2102 98-99 0.6679827285527828 2102 100-101 0.3284722616939497 2103 1 0.4771298301744835 2103 2 0.4490886726943444 2103 3 0.36881670323090887 2103 4 0.35908350920443866 2103 5 0.5017039679695827 2103 6 0.42806855261950716 2103 7 0.4195697909650917 2103 8 0.5062342417829271 2103 9 0.5110774243903933 2103 10-11 0.43421797661834916 2103 12-13 0.5182572221065413 2103 14-15 0.6890978898809337 2103 16-17 0.9278182646336504 2103 18-19 0.9366900908102522 2103 20-21 0.7533989492388784 2103 22-23 0.6446566979873225 2103 24-25 0.6577787365498509 2103 26-27 0.8506298079134069 2103 28-29 0.7753677804713632 2103 30-31 0.675216423499613 2103 32-33 0.6571296469309615 2103 34-35 0.6470670295743304 2103 36-37 0.5125302349360297 2103 38-39 0.5781745462521926 2103 40-41 0.7065904266822827 2103 42-43 0.631382010552592 2103 44-45 -1.1322900623297087 2103 46-47 -0.6591350421878488 2103 48-49 0.27849534009078525 2103 50-51 0.5479869860434832 2103 52-53 0.6361388368171603 2103 54-55 0.8033786246417307 2103 56-57 0.692511275309549 2103 58-59 0.7748006311041493 2103 60-61 0.7967228581495789 2103 62-63 0.7754435887547118 2103 64-65 0.6623312055566188 2103 66-67 0.782112045863677 2103 68-69 0.6803897021510181 2103 70-71 0.7167550365592135 2103 72-73 0.4618414987636008 2103 74-75 0.45674337546130417 2103 76-77 0.49679045862817617 2103 78-79 0.6868701387404492 2103 80-81 0.5698024282080354 2103 82-83 0.8269193641083135 2103 84-85 0.871568595897525 2103 86-87 0.7381937069100957 2103 88-89 0.6813206560852016 2103 90-91 0.6145637126333128 2103 92-93 0.8271339655537737 2103 94-95 0.9624581236135263 2103 96-97 0.9134671495240916 2103 98-99 0.6091430549587962 2103 100-101 0.8424450514086352 2104 1 0.38174675665906577 2104 2 0.35963466930871135 2104 3 0.25724659450321496 2104 4 0.3545440648863192 2104 5 0.36448894653565844 2104 6 0.2711334776218237 2104 7 0.6142605781670554 2104 8 0.6038280836350935 2104 9 0.2362136498298142 2104 10-11 0.4402270555069876 2104 12-13 0.5309196609419473 2104 14-15 0.5004709244025989 2104 16-17 0.4601270647549356 2104 18-19 0.5128036864038776 2104 20-21 0.5442392096463422 2104 22-23 0.4032113187037041 2104 24-25 0.4361818459428761 2104 26-27 0.44861527158522563 2104 28-29 0.3470556284041848 2104 30-31 0.4267239569559109 2104 32-33 0.6040417299379541 2104 34-35 0.7976140368256566 2104 36-37 0.6584430506548031 2104 38-39 0.8032033915152468 2104 40-41 0.9135457144190511 2104 42-43 0.6981872187016975 2104 44-45 0.7472525402288852 2104 46-47 0.8169612883578168 2104 48-49 0.9356725468586831 2104 50-51 1.064843568237741 2104 52-53 0.8850734570282199 2104 54-55 0.8023069283718272 2104 56-57 0.6697129900235836 2104 58-59 0.7242772053817461 2104 60-61 0.690997599805506 2104 62-63 0.8278998576515093 2104 64-65 0.8975864334973664 2104 66-67 0.8178085852743067 2104 68-69 0.7370843297734098 2104 70-71 0.7329820761509183 2104 72-73 0.5653096679105367 2104 74-75 0.7696365764011972 2104 76-77 0.4868609505557089 2104 78-79 0.5599509812747883 2104 80-81 0.541778307672736 2104 82-83 0.6166689788029736 2104 84-85 0.6278071735944515 2104 86-87 0.8437905301412698 2104 88-89 0.8331857023639522 2104 90-91 0.9078905610461909 2104 92-93 0.997683162518495 2104 94-95 0.6611851421502912 2104 96-97 0.5782115004846169 2104 98-99 0.6752829271132192 2104 100-101 0.8172675582936009 2105 1 0.45029720129823403 2105 2 0.48448965277477285 2105 3 0.4576415556112181 2105 4 0.4889000507113579 2105 5 0.6257022877048684 2105 6 0.6401470367410624 2105 7 0.661051797760507 2105 8 0.7412515577209717 2105 9 0.8816196316078759 2105 10-11 0.77740677278193 2105 12-13 0.6128867009858681 2105 14-15 0.8523751718474415 2105 16-17 1.0918768585939134 2105 18-19 0.9082609884922306 2105 20-21 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-2.188214493814872 2316 72-73 -1.9046103725802155 2316 74-75 -2.2126642913273784 2316 76-77 -2.696211772204279 2316 78-79 -2.992097646565526 2316 80-81 -2.87781444876466 2316 82-83 -2.5896820351692575 2316 84-85 -2.8245266415399435 2316 86-87 -2.778763842811685 2316 88-89 -2.9601016895349765 2316 90-91 -3.24412375671864 2316 92-93 -3.4655208631970247 2316 94-95 -3.371317692689278 2316 96-97 -3.182368169457323 2316 98-99 -3.9776487593364926 2316 100-101 -4.6802431362299295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2522.0 3 1063.0 4 979.0 5 1051.0 6 1245.0 7 1470.0 8 1820.0 9 2346.0 10 3054.0 11 3790.0 12 4789.0 13 5849.0 14 6645.0 15 7848.0 16 9748.0 17 11870.0 18 10713.0 19 7610.0 20 7609.0 21 8027.0 22 9018.0 23 10192.0 24 11718.0 25 13384.0 26 15969.0 27 18598.0 28 22617.0 29 26841.0 30 32637.0 31 40350.0 32 50305.0 33 64402.0 34 84866.0 35 115086.0 36 158671.0 37 206229.0 38 166398.0 39 26534.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T 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21.990466539820787 27.103445837704577 28.661716355067934 22.244371267406702 44-45 22.12682906882491 27.144908380971934 28.549979107309202 22.17828344289395 46-47 22.443456144782257 27.292859239141777 28.139989461986264 22.1236951540897 48-49 22.57188311287262 27.442151690885048 27.812806811671674 22.17315838457066 50-51 22.257944976168268 27.748273849836647 27.93458306179213 22.059198112202953 52-53 22.58164407099481 27.821193404156624 27.57099331649715 22.02616920835142 54-55 22.085060398104847 27.556350479815546 27.77601480274297 22.582574319336636 56-57 22.37492189074032 27.948866814075046 27.423921474583558 22.25228982060108 58-59 22.70639534759898 27.426309510067554 27.50327875346457 22.364016388868894 60-61 22.385031694391273 27.495917339868996 28.206645216195703 21.91240574954403 62-63 22.363820836932575 27.60478695038071 28.029625389101575 22.001766823585136 64-65 22.013732706331357 27.94788897975874 27.816227083759287 22.222151230150615 66-67 22.01629700937525 27.917807869078704 27.506037901408632 22.559857220137413 68-69 22.02466348611348 27.81679161697052 27.77342818197308 22.38511671494292 70-71 22.19657340512541 27.810710877862594 27.513972191930208 22.478743525081786 72-73 22.146249913210678 27.685304256744754 27.699616508330283 22.468829321714285 74-75 22.01715829616526 28.32912135398985 27.364079007730645 22.28964134211424 76-77 22.237348259835983 27.894846020392038 27.383575536880862 22.484230182891114 78-79 22.205860359168458 28.018497427054427 27.381364775380167 22.394277438396948 80-81 22.283860176660124 28.2854305456798 27.23508117478599 22.195628102874082 82-83 22.258788370317866 28.213514121367428 27.355378659833164 22.172318848481545 84-85 22.15298201013485 28.205179540830756 27.132228405516575 22.50961004351782 86-87 22.276225847990634 28.098095953320424 27.149672066413956 22.476006132274996 88-89 22.258907379609166 27.967451069260402 26.99049754558264 22.783144005547783 90-91 22.192842774658075 27.856444834087768 27.331150535518177 22.619561855735977 92-93 22.328826529960203 27.911650863460814 27.331139713744573 22.42838289283441 94-95 22.00161018248735 27.78565830053332 27.40811736441241 22.80461415256692 96-97 21.808617127766063 27.68040380806338 27.6606819585543 22.850297105616253 98-99 21.826611207656445 27.970103940909997 27.128547685986575 23.074737165446983 100-101 21.70261724775646 27.915150378823387 27.32752669660891 23.054705676811235 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 392.0 1 276.5 2 240.5 3 323.0 4 296.0 5 305.0 6 476.0 7 814.0 8 1246.5 9 1571.0 10 1495.5 11 1305.0 12 1404.5 13 1514.0 14 1560.0 15 1635.5 16 1713.0 17 1855.5 18 1999.5 19 2091.5 20 2236.5 21 2634.5 22 3153.0 23 3709.5 24 4462.0 25 5385.5 26 6669.0 27 8290.0 28 9903.0 29 11914.0 30 14364.0 31 16915.5 32 19689.0 33 21624.5 34 23388.0 35 25313.5 36 27395.5 37 30110.0 38 31758.0 39 32317.0 40 33218.5 41 35243.5 42 37319.0 43 39068.5 44 40670.0 45 42445.5 46 43422.5 47 45538.5 48 53104.0 49 52661.5 50 44582.5 51 40596.0 52 38023.5 53 36972.0 54 36189.5 55 33870.5 56 30790.0 57 27732.0 58 25457.5 59 22577.0 60 18871.5 61 15824.5 62 13344.0 63 10551.0 64 7830.0 65 6176.0 66 4696.0 67 3644.5 68 2640.0 69 1690.0 70 1252.0 71 965.0 72 765.5 73 672.5 74 562.5 75 451.0 76 325.0 77 195.0 78 120.0 79 81.5 80 55.5 81 40.0 82 28.5 83 17.0 84 8.0 85 6.5 86 8.5 87 5.5 88 2.0 89 4.0 90 3.5 91 2.0 92 1.0 93 0.0 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01686719892049927 2 0.006985405613540102 3 0.00749653285355523 4 0.03518259168770807 5 0.04583107585468993 6 0.0644020322419063 7 0.029645379920877502 8 0.027260119467473566 9 0.016270883807148286 10-11 0.04987749983814304 12-13 0.04600145160136165 14-15 0.022361816750661907 16-17 0.02568414381076025 18-19 0.03279733123430414 20-21 0.02355444697736388 22-23 0.016100508060476575 24-25 0.004429769413464455 26-27 0.0028537937567511386 28-29 0.0022574786434001546 30-31 0.0086891630802572 32-33 0.0016611635300491706 34-35 0.0025130422634077193 36-37 0.0068150298668683915 38-39 0.007453938916887303 40-41 0.004216799730124818 42-43 8.944726700264764E-4 44-45 0.0017463514033850254 46-47 0.0025982301367435743 48-49 7.240969233547666E-4 50-51 0.0017889453400529527 52-53 0.0013204120367057508 54-55 0.0033649209967662685 56-57 0.0033649209967662685 58-59 0.0025982301367435743 60-61 7.66690860022694E-4 62-63 0.001533381720045388 64-65 0.003066763440090776 66-67 0.003492702806770051 68-69 0.006474278373524972 70-71 0.009881793306959168 72-73 0.004813114843475802 74-75 0.003535296743437978 76-77 0.0030241695034228487 78-79 0.03539556137104771 80-81 0.02270256824400533 82-83 0.004429769413464455 84-85 0.00885953882692891 86-87 0.007368751043551448 88-89 0.006303902626853263 90-91 0.0130337446203858 92-93 0.014396750593759477 94-95 0.00817803584024207 96-97 0.0043445815401286 98-99 0.02355444697736388 100-101 0.043105063907942574 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1173876.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.59670102178596 #Duplication Level Percentage of deduplicated Percentage of total 1 83.42637891420533 53.8906885605115 2 10.33560681933892 13.352922071751369 3 2.7580135810311175 5.34475736123677 4 1.1477020032862635 2.9655105267355033 5 0.5886545695712527 1.9012571617851157 6 0.35398334061836667 1.3719693612370594 7 0.22797990648371508 1.0308724900672286 8 0.17198742237945644 0.8887856082362694 9 0.1361462190236882 0.7915136944967797 >10 0.7564182712520005 9.145469511303062 >50 0.06225722499839306 2.775417306062156 >100 0.03142433903913387 3.789330815314338 >500 0.0021214700137203515 0.8737154217716098 >1k 0.0013259187585752197 1.877790109491302 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4950 0.4216799730124818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4029 0.34322194167015935 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3016 0.2569266259809384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2328 0.19831736912587022 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1702 0.14498976041762504 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1290 0.1098923566032528 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1208 0.10290695098971273 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1179 0.10043650266297291 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 8.51878733358549E-5 0.0 12-13 0.0 0.0 0.0 8.51878733358549E-5 0.0 14-15 0.0 0.0 0.0 1.2778181000378235E-4 0.0 16-17 0.0 0.0 0.0 1.703757466717098E-4 0.0 18-19 0.0 0.0 0.0 1.703757466717098E-4 0.0 20-21 0.0 0.0 0.0 1.703757466717098E-4 0.0 22-23 0.0 0.0 0.0 2.1296968333963724E-4 0.0 24-25 0.0 0.0 0.0 2.981575566754921E-4 0.0 26-27 0.0 0.0 0.0 4.2593936667927447E-4 0.0 28-29 0.0 0.0 0.0 8.092847966906215E-4 0.0 30-31 0.0 0.0 0.0 0.0018741332133888075 0.0 32-33 0.0 0.0 0.0 0.00417420579345689 0.0 34-35 0.0 0.0 0.0 0.006048339006845697 8.51878733358549E-5 36-37 0.0 0.0 0.0 0.008603975206921344 8.51878733358549E-5 38-39 0.0 0.0 0.0 0.011457768963672482 8.51878733358549E-5 40-41 0.0 0.0 0.0 0.014567126340431186 8.51878733358549E-5 42-43 0.0 0.0 0.0 0.02155253195397129 8.51878733358549E-5 44-45 0.0 0.0 0.0 0.029773161730881285 8.51878733358549E-5 46-47 0.0 0.0 0.0 0.03701413096442895 8.51878733358549E-5 48-49 0.0 0.0 0.0 0.047023706081391906 8.51878733358549E-5 50-51 0.0 0.0 0.0 0.05971669920843428 8.51878733358549E-5 52-53 0.0 0.0 0.0 0.08663606718256442 8.51878733358549E-5 54-55 0.0 0.0 0.0 0.1090830718065622 8.51878733358549E-5 56-57 0.0 0.0 0.0 0.15048437824778765 8.51878733358549E-5 58-59 0.0 0.0 0.0 0.19746549039251166 8.51878733358549E-5 60-61 0.0 0.0 0.0 0.2464059236239603 8.51878733358549E-5 62-63 0.0 0.0 0.0 0.3132784041926064 8.51878733358549E-5 64-65 0.0 0.0 0.0 0.349312874613673 8.51878733358549E-5 66-67 0.0 0.0 0.0 0.44195468686641515 8.51878733358549E-5 68-69 0.0 0.0 0.0 0.5593009823865553 8.51878733358549E-5 70-71 0.0 0.0 0.0 0.6303476687486583 8.51878733358549E-5 72-73 0.0 0.0 0.0 0.7652426661759846 8.51878733358549E-5 74-75 0.0 0.0 0.0 0.8975394334665672 8.51878733358549E-5 76-77 0.0 0.0 0.0 0.9768067496055801 8.51878733358549E-5 78-79 0.0 0.0 0.0 1.0653595439382013 8.51878733358549E-5 80-81 0.0 0.0 0.0 1.231135145449775 8.51878733358549E-5 82-83 0.0 0.0 0.0 1.3658171731937616 8.51878733358549E-5 84-85 0.0 0.0 0.0 1.5919483829637884 8.51878733358549E-5 86-87 0.0 0.0 0.0 1.9281849190204077 8.51878733358549E-5 88-89 0.0 0.0 0.0 2.3763157267036723 8.51878733358549E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGATA 70 0.008378776 27.151396 9 GTACATG 5975 0.0 26.641314 1 TACATGG 6065 0.0 25.7748 2 ACATGGG 5930 0.0 25.079554 3 GAGTACT 4635 0.0 23.883575 12-13 CGTACAC 100 0.0016506416 23.757471 3 TACCTGG 990 0.0 22.557598 2 GTACTTT 4970 0.0 22.31961 14-15 GTGTAAG 395 0.0 21.6533 1 GTATCAA 10635 0.0 21.490984 1 CATGGGG 2510 0.0 21.20279 4 AGTACTT 4770 0.0 20.468529 12-13 GTACCTG 1095 0.0 20.395409 1 GTAAGGT 375 0.0 20.273905 4 CATGGGA 3485 0.0 19.906677 4 GTATAGG 410 0.0 19.702156 1 ACTTTTT 5985 0.0 19.12974 16-17 TATCAAC 11935 0.0 18.990053 2 TCAACGC 11910 0.0 18.911034 4 ATCAACG 11960 0.0 18.91063 3 >>END_MODULE