FastQCFastQC Report
Thu 26 May 2016
SRR936446_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936446_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173876
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69780.5944409801375955No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.5281648146823004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.3219249733361957No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31300.26663804354122583No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17010.14490457254428918No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT16950.14439344530427406No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13580.11568513199009095No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13480.1148332532567324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCC953.4951307E-529.9946868
GTATCAA114300.024.2386461
GTACATG60050.023.8276941
TACATGG61200.022.8980392
AGTACTT71750.022.7680312-13
ACATGGG59400.022.469433
GAGTACT69600.022.1749712-13
GTAAGGT4250.021.2342474
GGTATCA83500.021.0461851
GTACTTT73450.020.94753514-15
CATGGGG22350.020.8267144
GGACCGT1604.9962142E-520.7775696
TAAGGTG5500.020.7227175
ATCAACG134300.020.6895983
TCAACGC135350.020.5641864
TATCAAC135450.020.4464192
CAACGCA136750.020.4196435
AACGCAG139000.020.1924676
TACTTTT80750.020.02415714-15
TGCGTAA1803.6379788E-1219.78984860-61
Produced by FastQC (version 0.11.5)