FastQCFastQC Report
Thu 26 May 2016
SRR936445_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936445_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173974
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49190.419004168746497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40690.34660052096554095No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30640.2609938550598225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23620.20119696006896237No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17600.14991814128762646No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA12450.10605004880857667No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12250.10434643356667184No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12180.10375016823200513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG61400.027.0901341
TACATGG62300.025.9348532
GTACCTG8550.025.0125431
TACCTGG8100.024.6409362
ACATGGG62050.024.5842063
GAGTACT46000.022.36671812-13
AGTACTT47950.021.75444812-13
GTATCAA100400.021.5371931
CATGGGG24950.020.7610284
GTACTTT49150.020.54692514-15
TGCACCG2551.186163E-820.4987265
TGTACGG1404.8382196E-420.365625
ATGGGCG2151.0745334E-619.8925
GTATAGA3707.2759576E-1219.2664181
CATGGGA37500.019.008724
CATACGT1507.697393E-419.008724
TATCAAC117200.018.327522
ATCAACG116550.018.2258643
TACTTTT56050.018.14469114-15
TCAACGC116950.018.1228944
Produced by FastQC (version 0.11.5)