##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936445_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173974 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366013216647048 31.0 31.0 34.0 30.0 34.0 2 31.385195924270896 31.0 31.0 34.0 28.0 34.0 3 31.436977309548592 31.0 31.0 34.0 28.0 34.0 4 35.063835314921796 37.0 35.0 37.0 32.0 37.0 5 35.02894357115234 37.0 35.0 37.0 32.0 37.0 6 35.17864450149663 37.0 35.0 37.0 32.0 37.0 7 35.03417707717547 37.0 35.0 37.0 32.0 37.0 8 34.983353975471346 37.0 35.0 37.0 32.0 37.0 9 36.43304195833979 39.0 37.0 39.0 32.0 39.0 10-11 36.35560327571139 39.0 37.0 39.0 32.0 39.0 12-13 36.251334356638225 39.0 35.0 39.0 32.0 39.0 14-15 37.3080098026021 40.0 37.0 41.0 32.0 41.0 16-17 37.18528434190195 39.0 36.0 41.0 31.0 41.0 18-19 37.15894048760875 39.0 36.0 41.0 31.0 41.0 20-21 37.08833159848514 39.0 36.0 41.0 31.0 41.0 22-23 36.970019778972954 39.0 36.0 41.0 31.0 41.0 24-25 37.09933950837072 39.0 36.0 41.0 31.0 41.0 26-27 37.0905416133577 39.0 36.0 41.0 31.0 41.0 28-29 37.00218062750964 39.0 36.0 41.0 31.0 41.0 30-31 36.82260382257188 39.0 36.0 41.0 30.0 41.0 32-33 36.64577750444218 39.0 35.5 41.0 30.0 41.0 34-35 36.42856017254215 39.0 35.0 40.5 30.0 41.0 36-37 36.226079112484605 39.0 35.0 40.0 30.0 41.0 38-39 36.02618499217189 38.0 35.0 40.0 29.5 41.0 40-41 35.85175054984182 38.0 35.0 40.0 29.0 41.0 42-43 35.6228979517434 38.0 35.0 40.0 27.5 41.0 44-45 35.340409157272646 38.0 34.5 40.0 26.5 41.0 46-47 35.01983817358817 38.0 34.0 40.0 25.5 41.0 48-49 34.89587290689572 38.0 34.0 40.0 24.5 41.0 50-51 33.97005470308542 37.5 33.0 39.5 23.5 40.5 52-53 34.1012211514054 38.0 33.5 39.5 24.0 40.5 54-55 34.52431186721341 38.0 34.0 40.0 24.0 41.0 56-57 34.44114137110362 38.0 34.0 40.0 23.5 41.0 58-59 34.18043457521206 38.0 34.0 40.0 23.0 41.0 60-61 33.87081741162922 37.5 33.0 40.0 22.5 41.0 62-63 33.55246197956684 37.0 33.0 40.0 22.0 41.0 64-65 33.21160860461986 36.5 32.5 39.5 21.5 41.0 66-67 32.82693739384348 36.0 32.0 39.0 20.0 41.0 68-69 32.423540044328064 35.5 32.0 39.0 18.5 40.5 70-71 31.99219914580732 35.0 31.0 38.5 17.5 40.0 72-73 31.602245024165782 35.0 31.0 37.5 14.5 40.0 74-75 31.1889168755015 35.0 31.0 37.0 12.0 39.0 76-77 30.763508817060686 35.0 30.5 36.5 8.0 39.0 78-79 30.31291706630641 34.0 30.0 36.0 7.0 38.5 80-81 29.846614575791286 34.0 29.5 35.5 4.0 37.0 82-83 29.365359028394153 34.0 29.0 35.0 2.0 37.0 84-85 29.053503740287262 34.0 29.0 35.0 2.0 36.5 86-87 28.729971021504735 34.0 29.0 35.0 2.0 36.0 88-89 28.338018559184444 34.0 28.5 35.0 2.0 36.0 90-91 27.92315587909102 34.0 27.0 35.0 2.0 35.0 92-93 27.56598527735708 34.0 27.0 35.0 2.0 35.0 94-95 27.119753078006838 33.0 26.0 35.0 2.0 35.0 96-97 26.542221122443937 33.0 25.0 35.0 2.0 35.0 98-99 25.84362984188747 33.0 23.5 35.0 2.0 35.0 100-101 23.38125631402399 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4460088839938585 1101 2 0.5949443086495627 1101 3 0.6107530735056557 1101 4 0.6245690888383137 1101 5 0.5681586772294196 1101 6 0.527164794884385 1101 7 0.526000915059285 1101 8 0.5405216269493422 1101 9 0.7741316950982764 1101 10-11 0.5894298613022997 1101 12-13 0.6902088614777497 1101 14-15 0.9241415718845758 1101 16-17 0.8986156091767938 1101 18-19 1.0248599621588212 1101 20-21 1.100923126381332 1101 22-23 1.057710390970847 1101 24-25 0.9853396823996547 1101 26-27 0.8041919130232316 1101 28-29 0.8977635421514236 1101 30-31 0.8925871093072502 1101 32-33 0.7881111422822542 1101 34-35 0.6480822462665614 1101 36-37 0.8425525281933943 1101 38-39 0.7035173663303382 1101 40-41 0.7229544233294405 1101 42-43 0.8863910807674813 1101 44-45 0.891442777507983 1101 46-47 0.7743595228578428 1101 48-49 0.941498240259989 1101 50-51 0.9958488065381204 1101 52-53 1.0409513420296648 1101 54-55 1.1083765356185253 1101 56-57 0.8100402512967548 1101 58-59 0.7839290332839397 1101 60-61 0.8897530852979827 1101 62-63 0.9577708473478808 1101 64-65 0.9988010994559744 1101 66-67 1.0287900803799275 1101 68-69 0.88477003924136 1101 70-71 0.8015336582183146 1101 72-73 0.7743037617934583 1101 74-75 0.7449016459299287 1101 76-77 0.749187888561849 1101 78-79 0.6405356801102826 1101 80-81 0.7816028629017389 1101 82-83 1.0399583892129414 1101 84-85 0.8327737817486955 1101 86-87 0.6894118753767309 1101 88-89 0.8874459060392823 1101 90-91 0.8249422047086341 1101 92-93 0.663832849970273 1101 94-95 0.6290800642839969 1101 96-97 0.5425077468423751 1101 98-99 0.5776304384554898 1101 100-101 0.9306948453634369 1102 1 0.4156154757551036 1102 2 0.4708257379487115 1102 3 0.3371494448053056 1102 4 0.41443151740457296 1102 5 0.5545458047024141 1102 6 0.2820470515112703 1102 7 0.4338317792626398 1102 8 0.3066675020884304 1102 9 0.6066830804394172 1102 10-11 0.4984524027040962 1102 12-13 0.4047946965987279 1102 14-15 0.3661898087111979 1102 16-17 0.48944486226825745 1102 18-19 0.48976673433731577 1102 20-21 0.47318781669478227 1102 22-23 0.4018740056533119 1102 24-25 0.1624248027241677 1102 26-27 0.355423540130829 1102 28-29 0.5271781645001852 1102 30-31 0.34960961083782394 1102 32-33 0.23111812446664715 1102 34-35 0.019514887257216174 1102 36-37 0.10853445918785098 1102 38-39 0.23468327937855804 1102 40-41 0.26267061371869005 1102 42-43 0.4142670938767168 1102 44-45 0.27245316658284935 1102 46-47 0.30748856867144525 1102 48-49 0.5233525350981836 1102 50-51 0.37829718249431465 1102 52-53 0.3279905212701877 1102 54-55 0.5244856610381561 1102 56-57 0.6179546352051517 1102 58-59 0.6335203795512854 1102 60-61 0.6390344853232719 1102 62-63 0.539717213217699 1102 64-65 0.5683113182912365 1102 66-67 0.7124854286156506 1102 68-69 0.5603704813921624 1102 70-71 0.4878620805720111 1102 72-73 0.5210554380895509 1102 74-75 0.3475238603877564 1102 76-77 0.4135312699662279 1102 78-79 0.1658880026348477 1102 80-81 0.5350552642768776 1102 82-83 0.7802128934799484 1102 84-85 0.5924321903520635 1102 86-87 0.6634651569614256 1102 88-89 0.6302157069507608 1102 90-91 0.6854690188883055 1102 92-93 0.5323084632131803 1102 94-95 0.6341471187507004 1102 96-97 0.5416324162654043 1102 98-99 0.6467589953220738 1102 100-101 0.5222142377261036 1103 1 0.23238228627737811 1103 2 0.24551362678672106 1103 3 0.3180781692084871 1103 4 0.3587071280970662 1103 5 0.4489391364630535 1103 6 0.3090974564809912 1103 7 0.3181829719690228 1103 8 0.24862569607610396 1103 9 0.5088705182831532 1103 10-11 0.46736386443433986 1103 12-13 0.4149941054493169 1103 14-15 0.3556411684011067 1103 16-17 0.4053055212287049 1103 18-19 0.4953752528095734 1103 20-21 0.6003589493611017 1103 22-23 0.6215775983430447 1103 24-25 0.5891000729737996 1103 26-27 0.4296109409699085 1103 28-29 0.18071742875507368 1103 30-31 0.28265199504794225 1103 32-33 0.4394265952954015 1103 34-35 0.32061947629735954 1103 36-37 0.3293366276608083 1103 38-39 0.43975384263570305 1103 40-41 0.2815661171901951 1103 42-43 0.2982112353455193 1103 44-45 0.4703540773599926 1103 46-47 0.16824929192748783 1103 48-49 0.28759123364436334 1103 50-51 0.15569797531910723 1103 52-53 0.20285695758285982 1103 54-55 0.28996401562604035 1103 56-57 0.15465631752941533 1103 58-59 -0.1558491073328767 1103 60-61 -0.13892600295319113 1103 62-63 -0.11015165152564776 1103 64-65 0.11098000171176636 1103 66-67 -0.0013737409073115714 1103 68-69 0.24176731471855817 1103 70-71 0.3189843855921559 1103 72-73 0.24058557144508796 1103 74-75 0.285344471057126 1103 76-77 0.18757389008103686 1103 78-79 0.10149064661765905 1103 80-81 0.20216829963020544 1103 82-83 -0.1387654079137377 1103 84-85 -0.037107240423786436 1103 86-87 0.04513813754785545 1103 88-89 -0.04702504072835012 1103 90-91 -0.15719873941576878 1103 92-93 -0.12313191534446943 1103 94-95 -0.14948664614945528 1103 96-97 0.07921989758961345 1103 98-99 0.16179213761886047 1103 100-101 0.8575157563047533 1104 1 0.23709383666085415 1104 2 0.2296165723913397 1104 3 0.3014017606143362 1104 4 0.3180478652952914 1104 5 0.3690287621959101 1104 6 0.14438803160896896 1104 7 0.4323229368980819 1104 8 0.3578681283697023 1104 9 0.5333316125672951 1104 10-11 0.4661638360941893 1104 12-13 0.3149223694378449 1104 14-15 0.5094529189369013 1104 16-17 0.41325847657525117 1104 18-19 0.5369777113178245 1104 20-21 0.535362580443163 1104 22-23 0.29565698619574476 1104 24-25 0.23797886568330284 1104 26-27 0.024590305796238 1104 28-29 0.31843728634580515 1104 30-31 0.49405182146450244 1104 32-33 0.2162921744087143 1104 34-35 0.0356813818183781 1104 36-37 0.2981934567519815 1104 38-39 0.2149411533323402 1104 40-41 0.4973542391237018 1104 42-43 0.2963735535700138 1104 44-45 0.3925249330554834 1104 46-47 0.43802983287014996 1104 48-49 0.40580066737268794 1104 50-51 0.4700124093131208 1104 52-53 0.6379225827489208 1104 54-55 0.6508050096568994 1104 56-57 0.5301323435315055 1104 58-59 0.507159908378199 1104 60-61 0.5835530519027188 1104 62-63 0.7577345273361331 1104 64-65 0.5556945632364574 1104 66-67 0.6847236429423447 1104 68-69 0.4519671127949749 1104 70-71 0.43940346559994836 1104 72-73 0.4853780683896609 1104 74-75 0.3040976814650449 1104 76-77 0.6537428328162171 1104 78-79 0.6261775764934931 1104 80-81 0.576984531452652 1104 82-83 0.5832531194610802 1104 84-85 0.6106822276669774 1104 86-87 0.5443277863070897 1104 88-89 0.6145802517951608 1104 90-91 0.41302727330187494 1104 92-93 0.5275402776519691 1104 94-95 0.5159578514327734 1104 96-97 0.6348773005688706 1104 98-99 0.8120170875807808 1104 100-101 1.2707993172449399 1105 1 0.239703200494084 1105 2 0.3403248513181474 1105 3 0.2699341009278271 1105 4 0.24632636222434456 1105 5 0.33413819319768834 1105 6 0.15947892409092645 1105 7 0.13767331492268653 1105 8 0.23188518380793965 1105 9 0.33041058666173484 1105 10-11 0.20200846717764875 1105 12-13 0.18813796973660857 1105 14-15 -0.05495016759230964 1105 16-17 0.3232553062196857 1105 18-19 0.1973694920366924 1105 20-21 0.2487796326573175 1105 22-23 0.07421033683169753 1105 24-25 0.23201456711103674 1105 26-27 0.0016818001084075718 1105 28-29 -0.08430668326176516 1105 30-31 0.048722185088166725 1105 32-33 0.11363122512902635 1105 34-35 -0.2620614862249724 1105 36-37 -0.21486384070797726 1105 38-39 0.024192412070974 1105 40-41 -0.08274157009612537 1105 42-43 0.08673701602816664 1105 44-45 -0.1745350726404098 1105 46-47 0.07533846967099578 1105 48-49 0.05813867026362374 1105 50-51 0.2881288419645571 1105 52-53 0.1935940264428595 1105 54-55 0.13794733712775553 1105 56-57 0.3396146620823828 1105 58-59 0.497627347751866 1105 60-61 0.06091080234737234 1105 62-63 0.4186432372163651 1105 64-65 0.4242803697158095 1105 66-67 0.2801142683621478 1105 68-69 0.2596444780682958 1105 70-71 0.4067659984453691 1105 72-73 0.4827044914225702 1105 74-75 0.4556919941695732 1105 76-77 0.15156334770345126 1105 78-79 0.35056002884412507 1105 80-81 0.17743859085114266 1105 82-83 0.173517441139321 1105 84-85 0.4096749680900835 1105 86-87 0.4668144735004631 1105 88-89 0.3045869149153262 1105 90-91 0.4687280024836511 1105 92-93 0.33917010908577794 1105 94-95 0.08997383217420918 1105 96-97 0.30054046909745225 1105 98-99 0.44864517263228265 1105 100-101 0.924428030994072 1106 1 0.04788251218509032 1106 2 0.14031692149101715 1106 3 0.08445056446891996 1106 4 0.13257055210100077 1106 5 0.17416735056205823 1106 6 0.22393438896179418 1106 7 0.21670014213785294 1106 8 0.1560644467000074 1106 9 0.31842617792184313 1106 10-11 0.36972797089246257 1106 12-13 0.4762127297068375 1106 14-15 0.5550987533412197 1106 16-17 0.5792911246388499 1106 18-19 0.5733317460595515 1106 20-21 0.3967584417192711 1106 22-23 0.309241263157503 1106 24-25 0.29898134723523384 1106 26-27 0.06326758253835152 1106 28-29 0.3528174124329482 1106 30-31 0.477061252296501 1106 32-33 0.2191189704961829 1106 34-35 0.21249779838262128 1106 36-37 0.2588069982677368 1106 38-39 0.37829304767711136 1106 40-41 0.21364181874599808 1106 42-43 0.23613983904662206 1106 44-45 0.22821383243345394 1106 46-47 0.3869196772006731 1106 48-49 0.48838120097645543 1106 50-51 0.4000458471738497 1106 52-53 0.43442714628084644 1106 54-55 0.5058416020327812 1106 56-57 0.6525427202507146 1106 58-59 0.6794186347018112 1106 60-61 0.5970275050032541 1106 62-63 0.7845519954054296 1106 64-65 0.573765845932428 1106 66-67 0.7440735090507005 1106 68-69 0.8485521748805027 1106 70-71 0.9606894329145028 1106 72-73 0.8948837909138412 1106 74-75 0.6443617961678889 1106 76-77 0.7389924367493137 1106 78-79 0.8300007320367939 1106 80-81 0.8828025089177309 1106 82-83 1.1361636854511978 1106 84-85 1.1732654718171034 1106 86-87 1.4238656147855835 1106 88-89 1.32901017248831 1106 90-91 1.4956866697847069 1106 92-93 1.4841913976216894 1106 94-95 1.5087900919797441 1106 96-97 1.5015768365046434 1106 98-99 1.8663033726835216 1106 100-101 2.010539548762985 1107 1 0.08026787926100809 1107 2 0.06847627518245503 1107 3 -0.15047242974378605 1107 4 0.033214111760720755 1107 5 0.17617246887437688 1107 6 0.0762629291480863 1107 7 0.2535068650313548 1107 8 0.05709601530217867 1107 9 0.2361141554830084 1107 10-11 0.08147746550778123 1107 12-13 0.2374911401108335 1107 14-15 0.007259318496309675 1107 16-17 0.03704193775476483 1107 18-19 0.0016017856875478742 1107 20-21 0.4193848973609704 1107 22-23 0.4764810234920418 1107 24-25 0.3093079802176888 1107 26-27 0.12786042857786128 1107 28-29 0.24110945545206874 1107 30-31 0.22862697176725533 1107 32-33 -0.027279175331933914 1107 34-35 0.09359495144303054 1107 36-37 0.09271210757921722 1107 38-39 -0.17170871599989113 1107 40-41 -0.25128229448936423 1107 42-43 -0.058097437544148534 1107 44-45 -0.01151274897665644 1107 46-47 0.03468540250980112 1107 48-49 -0.21628743452137655 1107 50-51 -0.23321667554109382 1107 52-53 -0.1329641686437455 1107 54-55 -0.13926188323625155 1107 56-57 -0.24598138617320586 1107 58-59 -0.2609940170656415 1107 60-61 -0.30191684308091027 1107 62-63 -0.03984750298049988 1107 64-65 0.12849000684788336 1107 66-67 -0.10146821954738527 1107 68-69 -0.11845338131024619 1107 70-71 -0.016728811533567978 1107 72-73 -0.254281016796714 1107 74-75 -0.1929490342685547 1107 76-77 -0.3582637075364943 1107 78-79 -0.06106332471225073 1107 80-81 0.08174193086955128 1107 82-83 0.1517162758670132 1107 84-85 0.1267901499156423 1107 86-87 -0.14989652721839164 1107 88-89 -0.3310629775361349 1107 90-91 -0.251738665861275 1107 92-93 -0.2681658479283726 1107 94-95 -0.18480386821710937 1107 96-97 -0.2830690779679692 1107 98-99 -0.1583640829752042 1107 100-101 0.516064419041502 1108 1 0.07220804303688055 1108 2 0.27635682308855536 1108 3 0.1884707582704408 1108 4 0.13080605179929705 1108 5 0.2384313389197672 1108 6 0.051876838265684455 1108 7 0.04204055076472457 1108 8 0.2763241866051587 1108 9 0.09808343483035742 1108 10-11 0.03384492893490432 1108 12-13 0.12445123280990344 1108 14-15 0.07677924022043214 1108 16-17 0.2876386786387002 1108 18-19 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2316 76-77 -2.771879918170022 2316 78-79 -3.5926035240784913 2316 80-81 -3.00171642340754 2316 82-83 -2.9303872876698414 2316 84-85 -2.6321151175723543 2316 86-87 -3.3674728404057532 2316 88-89 -3.372255820325414 2316 90-91 -3.458961980030839 2316 92-93 -4.119892016865034 2316 94-95 -4.365351800916976 2316 96-97 -5.243904159211418 2316 98-99 -6.305324644183589 2316 100-101 -6.811925827125105 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2502.0 3 1038.0 4 1015.0 5 1143.0 6 1233.0 7 1458.0 8 1841.0 9 2254.0 10 2958.0 11 3707.0 12 4676.0 13 5765.0 14 6673.0 15 8028.0 16 9843.0 17 12333.0 18 10670.0 19 7760.0 20 7623.0 21 8237.0 22 8973.0 23 10492.0 24 11820.0 25 13709.0 26 15898.0 27 19037.0 28 22792.0 29 27143.0 30 32845.0 31 40636.0 32 50679.0 33 63867.0 34 84099.0 35 114990.0 36 159304.0 37 208177.0 38 164145.0 39 24597.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65003638619472 18.720569362994475 14.340165757437868 22.28922849337294 2 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22.112254094380738 27.09267875487893 28.582508607867958 22.21255854287238 46-47 22.40395684878035 27.285152148978952 28.174276138673697 22.136614863567 48-49 22.558876956265266 27.419209891371242 27.865948579691256 22.155964572672232 50-51 22.23527863467879 27.731607104297357 27.939151533839794 22.09396272718406 52-53 22.60174229785175 27.804656151091717 27.56992766478166 22.023673886274878 54-55 22.037388917924385 27.51828277962143 27.77069993155006 22.67362837090413 56-57 22.390597276567103 27.90255622698256 27.458704399113067 22.248142097337272 58-59 22.72996018916324 27.370951294129075 27.49137204688018 22.40771646982751 60-61 22.350812834279267 27.434285743495025 28.25798223538372 21.956919186841983 62-63 22.376034184306295 27.523869723445145 28.05391047746262 22.04618561478594 64-65 22.076212931461857 27.886818617788812 27.77578549439766 22.261182956351675 66-67 22.00568979742396 27.917916643062114 27.514246855767173 22.562146703746755 68-69 22.012174056029284 27.750185200883852 27.85987865135417 22.37776209173269 70-71 22.197972477107204 27.708897427411554 27.563782295783568 22.529347799697675 72-73 22.139121568353527 27.624517172660205 27.7247774490191 22.511583809967167 74-75 21.979906791637312 28.26082326955037 27.405490451296462 22.353779487515855 76-77 22.254839365067465 27.75911000065168 27.452266548313574 22.53378408596728 78-79 22.212406945354275 27.89918622173648 27.430920848074 22.457485984835255 80-81 22.34003492780168 28.173531541508712 27.262001107466883 22.22443242322273 82-83 22.30333558656122 28.146394217765742 27.356955169283857 22.193315026389175 84-85 22.198423307714002 28.116812910712323 27.12143325128672 22.563330530286958 86-87 22.247172346819035 28.067271631466973 27.172559784819374 22.512996236894615 88-89 22.223670412367884 27.954840319998908 26.964959761610864 22.85652950602234 90-91 22.148910777733576 27.798487278848228 27.415919946231014 22.636681997187182 92-93 22.30093087213671 27.927612465473555 27.390311977630443 22.381144684759292 94-95 21.97878684614074 27.770489010052817 27.443431589708638 22.807292554097803 96-97 21.81648031806977 27.678953647907427 27.637848905783247 22.866717128239557 98-99 21.829111133358754 27.965100551055144 27.07033355131288 23.135454764273224 100-101 21.713152078645486 27.836494971957066 27.313472837149018 23.136880112248434 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 318.0 1 230.0 2 213.5 3 291.0 4 282.5 5 294.5 6 448.0 7 836.0 8 1262.0 9 1512.5 10 1447.5 11 1295.5 12 1335.0 13 1454.5 14 1580.5 15 1654.0 16 1751.5 17 1846.0 18 1955.0 19 2115.0 20 2280.0 21 2663.5 22 3180.5 23 3742.5 24 4443.5 25 5369.5 26 6725.0 27 8331.0 28 9889.0 29 11718.0 30 14075.5 31 16688.0 32 19714.0 33 21772.0 34 23364.0 35 25302.5 36 27556.5 37 30185.0 38 31670.5 39 32298.5 40 33157.0 41 35148.0 42 37194.0 43 38933.0 44 40827.0 45 42463.5 46 43162.0 47 45637.5 48 53327.0 49 52779.5 50 44862.0 51 40795.5 52 38127.5 53 37218.5 54 36501.0 55 34113.5 56 31034.0 57 27868.5 58 25244.0 59 22478.0 60 18856.5 61 15643.5 62 13201.5 63 10521.5 64 7904.0 65 6145.5 66 4556.5 67 3564.0 68 2582.0 69 1629.5 70 1223.0 71 990.5 72 803.5 73 682.0 74 548.0 75 421.5 76 322.5 77 209.0 78 121.5 79 79.5 80 52.5 81 33.5 82 24.5 83 13.5 84 9.5 85 7.0 86 3.0 87 2.0 88 2.5 89 4.5 90 3.5 91 4.0 92 3.5 93 0.5 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03850170446704952 2 0.026235674725334633 3 0.025980132439048908 4 0.05971171422876487 5 0.0642262946198127 6 0.05715629136590759 7 0.05945617194247914 8 0.051960264878097816 9 0.05161954182971684 10-11 0.06213936594847926 12-13 0.0565600260312409 14-15 0.04923448049105006 16-17 0.06115978718438398 18-19 0.06345966776095552 20-21 0.015204766034000752 22-23 0.02683194006000133 24-25 0.008390305066381368 26-27 0.014140006507810225 28-29 0.00847548582847661 30-31 0.012010487455429165 32-33 0.006175605251905069 34-35 0.0110734990723815 36-37 0.012819704695333969 38-39 0.01656765822752463 40-41 0.003449820864857314 42-43 0.003066507435428723 44-45 0.004088676580571631 46-47 8.09217239904802E-4 48-49 0.0018313863850477098 50-51 7.240364778095597E-4 52-53 0.0054941591551431294 54-55 0.010519824118762426 56-57 0.02312657690885829 58-59 0.01473627184247692 60-61 0.015332537177143618 62-63 0.008688437733714716 64-65 0.0 66-67 0.010093920308286214 68-69 0.021167419380667716 70-71 0.006686689824476521 72-73 0.002512832481809648 74-75 0.0038331342942859044 76-77 0.0072829551591432175 78-79 0.0057071110603812356 80-81 0.010562414499810047 82-83 0.008731028114762337 84-85 0.02138037128590582 86-87 0.006516328300286037 88-89 0.00809217239904802 90-91 0.005366388012000266 92-93 0.020102659854477185 94-95 0.014821452604572164 96-97 0.012223439360667271 98-99 0.02695971120314419 100-101 0.04305887523914499 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1173974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.80076978660895 #Duplication Level Percentage of deduplicated Percentage of total 1 83.67211593227681 54.22017522085925 2 10.122582306349237 13.119022513594757 3 2.7478332265319905 5.341851249734832 4 1.110644828363184 2.8788255934980196 5 0.5913131359838608 1.9158773198343977 6 0.3572049869832153 1.3888294876876788 7 0.2471784499492884 1.1212147681962275 8 0.17013892471561515 0.8820106633790215 9 0.13088255711840058 0.7633161407620955 >10 0.7525076373680017 9.077639401543768 >50 0.06249670832351392 2.7349481986714177 >100 0.03180231695032143 3.838413763973093 >500 0.0019793934519917356 0.830994080083786 >1k 0.0013195956346611572 1.8868815981816032 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4919 0.419004168746497 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4069 0.34660052096554095 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3064 0.2609938550598225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2362 0.20119696006896237 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1760 0.14991814128762646 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1245 0.10605004880857667 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1225 0.10434643356667184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1218 0.10375016823200513 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 4.2590381047621154E-5 0.0 16-17 0.0 0.0 0.0 8.518076209524231E-5 0.0 18-19 0.0 0.0 0.0 8.518076209524231E-5 0.0 20-21 0.0 0.0 0.0 1.7036152419048462E-4 0.0 22-23 0.0 0.0 0.0 2.5554228628572694E-4 0.0 24-25 0.0 0.0 0.0 3.4072304838096923E-4 0.0 26-27 0.0 0.0 0.0 5.110845725714539E-4 0.0 28-29 0.0 0.0 0.0 0.0011925306693333923 0.0 30-31 0.0 0.0 0.0 0.0020443382902858155 0.0 32-33 0.0 0.0 0.0 0.0031090978164763448 0.0 34-35 0.0 0.0 0.0 0.004259038104762115 8.518076209524231E-5 36-37 0.0 0.0 0.0 0.006558918681333658 8.518076209524231E-5 38-39 0.0 0.0 0.0 0.009710606878857624 8.518076209524231E-5 40-41 0.0 0.0 0.0 0.012691933552191104 8.518076209524231E-5 42-43 0.0 0.0 0.0 0.019548984900858112 8.518076209524231E-5 44-45 0.0 0.0 0.0 0.027428205394668025 8.518076209524231E-5 46-47 0.0 0.0 0.0 0.03637218541466847 8.518076209524231E-5 48-49 0.0 0.0 0.0 0.04778640753543094 8.518076209524231E-5 50-51 0.0 0.0 0.0 0.0609894256601935 8.518076209524231E-5 52-53 0.0 0.0 0.0 0.08760841381495671 8.518076209524231E-5 54-55 0.0 0.0 0.0 0.11158679834476742 8.518076209524231E-5 56-57 0.0 0.0 0.0 0.15494380625124576 8.518076209524231E-5 58-59 0.0 0.0 0.0 0.20379497331286722 8.518076209524231E-5 60-61 0.0 0.0 0.0 0.25290168266077445 8.518076209524231E-5 62-63 0.0 0.0 0.0 0.32611454768163517 8.518076209524231E-5 64-65 0.0 0.0 0.0 0.3645736617676371 8.518076209524231E-5 66-67 0.0 0.0 0.0 0.46261671893926104 8.518076209524231E-5 68-69 0.0 0.0 0.0 0.5838289434007908 8.518076209524231E-5 70-71 0.0 0.0 0.0 0.6612582561453662 8.518076209524231E-5 72-73 0.0 0.0 0.0 0.8029990442718493 8.518076209524231E-5 74-75 0.0 0.0 0.0 0.9412900115334752 8.518076209524231E-5 76-77 0.0 0.0 0.0 1.0229783623828126 8.518076209524231E-5 78-79 0.0 0.0 0.0 1.108712799431674 8.518076209524231E-5 80-81 0.0 0.0 0.0 1.283077819440635 8.518076209524231E-5 82-83 0.0 0.0 0.0 1.4261814997606421 8.518076209524231E-5 84-85 0.0 0.0 0.0 1.6628988376233205 8.518076209524231E-5 86-87 0.0 0.0 0.0 2.003451524480099 8.518076209524231E-5 88-89 0.0 0.0 0.0 2.4540151655828835 8.518076209524231E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6140 0.0 27.090134 1 TACATGG 6230 0.0 25.934853 2 GTACCTG 855 0.0 25.012543 1 TACCTGG 810 0.0 24.640936 2 ACATGGG 6205 0.0 24.584206 3 GAGTACT 4600 0.0 22.366718 12-13 AGTACTT 4795 0.0 21.754448 12-13 GTATCAA 10040 0.0 21.537193 1 CATGGGG 2495 0.0 20.761028 4 GTACTTT 4915 0.0 20.546925 14-15 TGCACCG 255 1.186163E-8 20.498726 5 TGTACGG 140 4.8382196E-4 20.36562 5 ATGGGCG 215 1.0745334E-6 19.892 5 GTATAGA 370 7.2759576E-12 19.266418 1 CATGGGA 3750 0.0 19.00872 4 CATACGT 150 7.697393E-4 19.00872 4 TATCAAC 11720 0.0 18.32752 2 ATCAACG 11655 0.0 18.225864 3 TACTTTT 5605 0.0 18.144691 14-15 TCAACGC 11695 0.0 18.122894 4 >>END_MODULE