FastQCFastQC Report
Thu 26 May 2016
SRR936445_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936445_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1173974
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.589876777509553No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62280.5305057863291691No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.33263087598192126No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32160.2739413308982993No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT17950.15289946796095996No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.14421103022724524No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13900.1184012593123868No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC12910.10996836386495783No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG57050.024.8207931
TACATGG58500.024.1983622
GAGTACT66500.023.46401212-13
GGTATCA85100.023.4517251
ACATGGG56100.023.2850343
GTATCAA110050.022.2799761
GTACTTT70450.021.97987214-15
ATGGGCG1604.989851E-520.7810085
GTACACG1150.00371531320.6598531
TATTCCG1150.003723576220.6519325
GTAAGGT4900.020.3569074
TAAGGTG5650.020.1767565
AGTACTT68000.019.90785612-13
TAGACCG1200.004763880719.7914355
CATGGGA34600.019.6312734
TCAACGC130500.018.8905834
TATCAAC130350.018.8774912
ACTTTTT83050.018.84535416-17
GTGTAAG5300.018.8277531
ATCAACG130900.018.7610863
Produced by FastQC (version 0.11.5)