##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936445_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1173974 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.857220858383577 31.0 31.0 34.0 30.0 34.0 2 31.998295532950475 31.0 31.0 34.0 30.0 34.0 3 32.09965041815236 33.0 31.0 34.0 30.0 34.0 4 35.857444883787885 37.0 35.0 37.0 35.0 37.0 5 35.63253104412875 37.0 35.0 37.0 33.0 37.0 6 35.74667326533637 37.0 35.0 37.0 33.0 37.0 7 35.692095395639086 37.0 35.0 37.0 33.0 37.0 8 35.66581968595557 37.0 35.0 37.0 33.0 37.0 9 37.298851592965434 39.0 37.0 39.0 34.0 39.0 10-11 37.17133684391648 39.0 37.0 39.0 33.0 39.0 12-13 37.072470514679196 39.0 37.0 39.0 33.0 39.0 14-15 38.147352922637126 40.0 37.0 41.0 33.0 41.0 16-17 38.08833969065755 40.0 37.0 41.0 33.0 41.0 18-19 38.25619860405767 40.0 38.0 41.0 33.5 41.0 20-21 38.30277033392562 40.0 38.0 41.0 34.0 41.0 22-23 38.21522708339367 40.0 38.0 41.0 33.0 41.0 24-25 38.08475400647714 40.0 37.0 41.0 33.0 41.0 26-27 37.85759863506347 40.0 37.0 41.0 33.0 41.0 28-29 37.772346747031875 40.0 37.0 41.0 33.0 41.0 30-31 37.70085070027105 40.0 37.0 41.0 33.0 41.0 32-33 37.51978493561187 40.0 36.0 41.0 32.5 41.0 34-35 37.3355168002017 39.0 36.0 41.0 32.0 41.0 36-37 37.067239138175125 39.0 36.0 41.0 31.0 41.0 38-39 36.8846597113735 39.0 35.0 41.0 31.0 41.0 40-41 36.64673152897764 39.0 35.0 41.0 30.5 41.0 42-43 36.37842533139575 39.0 35.0 40.0 30.0 41.0 44-45 36.087037276805106 39.0 35.0 40.0 30.0 41.0 46-47 35.65431304270793 38.5 35.0 40.0 28.5 41.0 48-49 35.22762940235474 38.0 35.0 40.0 26.5 41.0 50-51 34.889492441910974 38.0 34.0 40.0 26.0 41.0 52-53 34.61228655830538 38.0 34.0 40.0 24.5 41.0 54-55 34.346462102227136 38.0 34.0 40.0 24.0 41.0 56-57 34.02272622732701 38.0 33.0 40.0 23.0 41.0 58-59 33.689888787997006 37.0 33.0 40.0 22.5 41.0 60-61 33.33766207769507 37.0 32.5 39.5 22.0 41.0 62-63 32.93993010066662 36.0 32.0 39.0 20.5 40.0 64-65 32.53163443142694 36.0 31.5 39.0 19.5 40.0 66-67 32.076236356171435 35.0 31.0 38.5 18.5 40.0 68-69 31.80596163117752 35.0 31.0 38.0 18.0 40.0 70-71 31.37409261193178 35.0 30.5 37.5 15.0 39.5 72-73 30.921092801033073 34.5 30.0 37.0 12.0 39.0 74-75 30.401206500314316 34.0 29.5 36.0 8.0 39.0 76-77 28.980614136258552 32.5 28.0 35.0 7.0 37.0 78-79 29.75980089848668 34.0 29.0 35.0 7.0 37.5 80-81 29.55783049709789 34.0 29.0 35.0 3.5 37.0 82-83 29.220544492467464 34.0 29.0 35.0 2.0 37.0 84-85 28.79719440123887 34.0 29.0 35.0 2.0 36.0 86-87 28.41183407809713 33.5 28.5 35.0 2.0 36.0 88-89 28.00267339821836 33.0 27.0 35.0 2.0 35.0 90-91 27.509051733684053 33.0 26.5 35.0 2.0 35.0 92-93 27.028512130592333 33.0 26.0 34.5 2.0 35.0 94-95 26.428141935000262 32.0 25.0 34.0 2.0 35.0 96-97 25.669694132919467 32.0 23.5 34.0 2.0 35.0 98-99 24.72743731973621 32.0 17.5 34.0 2.0 35.0 100-101 21.53176135076245 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36944038396579515 1101 2 0.43206212437203106 1101 3 0.4107701167314701 1101 4 0.3229341344528862 1101 5 0.26936982550818556 1101 6 0.2579738312667814 1101 7 0.24119427943808347 1101 8 0.3287984998475224 1101 9 0.41445223164915035 1101 10-11 0.35405174333600087 1101 12-13 0.4655150927628213 1101 14-15 0.6337315471362572 1101 16-17 0.5878755961871818 1101 18-19 0.4244061000730852 1101 20-21 0.3672200999228252 1101 22-23 0.3645933700516508 1101 24-25 0.3127242663247074 1101 26-27 0.45533674067505103 1101 28-29 0.34993372820920854 1101 30-31 0.48599342748757124 1101 32-33 0.5191633759013428 1101 34-35 0.3608494561350355 1101 36-37 0.3521482380026413 1101 38-39 0.474948966781362 1101 40-41 0.39597591037564683 1101 42-43 0.503419406443335 1101 44-45 0.5659996139934336 1101 46-47 0.6345597981550455 1101 48-49 0.4583858239372489 1101 50-51 0.5777482502600506 1101 52-53 0.5248428360824704 1101 54-55 0.7585464507524122 1101 56-57 0.57177554804354 1101 58-59 0.6351001108220018 1101 60-61 0.6305577299351484 1101 62-63 0.8240536740820801 1101 64-65 0.8487967385482378 1101 66-67 0.6962467832944128 1101 68-69 0.7714030253836484 1101 70-71 0.7660003092994607 1101 72-73 0.7195071784160021 1101 74-75 0.660951438886002 1101 76-77 0.5858906707920042 1101 78-79 0.46020156527552203 1101 80-81 0.428940582515672 1101 82-83 0.8001884532855996 1101 84-85 0.8603560218147805 1101 86-87 0.708248774941989 1101 88-89 0.6733031841631743 1101 90-91 0.744935454330502 1101 92-93 0.7130967606055734 1101 94-95 0.8332269575640581 1101 96-97 0.853674284425967 1101 98-99 0.9183629289964763 1101 100-101 1.0393700315404146 1102 1 0.25567206615863114 1102 2 0.2758650619363294 1102 3 0.2110436542578924 1102 4 0.09851689686713172 1102 5 0.01582456616953465 1102 6 0.17338652196005455 1102 7 0.21503194168376893 1102 8 0.18931167387436432 1102 9 0.3086508123387901 1102 10-11 0.2998497106384548 1102 12-13 0.2126340039428598 1102 14-15 0.4306170805475489 1102 16-17 0.3761145842557241 1102 18-19 0.31236562239493537 1102 20-21 0.2541503153270668 1102 22-23 0.0940414632369766 1102 24-25 0.22623179490214085 1102 26-27 -0.025937690281381265 1102 28-29 0.11681328618431053 1102 30-31 0.2416956671482069 1102 32-33 0.27099863223391196 1102 34-35 0.24338491922084415 1102 36-37 0.4908961349271479 1102 38-39 0.39685725563992946 1102 40-41 0.4101884250060195 1102 42-43 0.35710568397743003 1102 44-45 0.4265699717379263 1102 46-47 0.37885624694125397 1102 48-49 0.45525068727078377 1102 50-51 0.40007057021921355 1102 52-53 0.24634344175771616 1102 54-55 0.4850501071003066 1102 56-57 0.30176216495512875 1102 58-59 0.24033467685791976 1102 60-61 0.37722441758666037 1102 62-63 0.21298715577830052 1102 64-65 0.22989657554841614 1102 66-67 0.18732743489499626 1102 68-69 0.10780119613423267 1102 70-71 0.3040584341876489 1102 72-73 0.30105614367925426 1102 74-75 0.20430430386894827 1102 76-77 0.08981562476960292 1102 78-79 0.08240553067779999 1102 80-81 0.3424753913987679 1102 82-83 0.297105778745415 1102 84-85 0.16266404059831885 1102 86-87 0.2521997081645253 1102 88-89 0.062209385553735785 1102 90-91 0.3168814896889245 1102 92-93 0.2662044333489284 1102 94-95 0.2642088334732904 1102 96-97 0.4356930480300498 1102 98-99 0.45639010123016277 1102 100-101 1.111012048877047 1103 1 0.13163707825452775 1103 2 0.10735510074496801 1103 3 0.1783130342911008 1103 4 0.09718472918052612 1103 5 -0.08828341342239554 1103 6 0.09949284559353799 1103 7 0.02824167945951217 1103 8 0.039734220812462695 1103 9 0.16978620775668674 1103 10-11 0.040389238551533424 1103 12-13 0.14528783924438216 1103 14-15 0.09501489912295824 1103 16-17 0.24646182671486372 1103 18-19 0.17591788453593438 1103 20-21 0.11963144526430369 1103 22-23 0.04083570346196552 1103 24-25 0.03621750185300243 1103 26-27 0.15671267583345383 1103 28-29 0.2548314092155479 1103 30-31 0.10593945383484993 1103 32-33 0.08513117366756973 1103 34-35 0.14882414138293854 1103 36-37 0.30888641195326727 1103 38-39 0.21460468959818257 1103 40-41 0.20332776141999886 1103 42-43 0.22250124064311905 1103 44-45 0.21491489215478765 1103 46-47 0.07650043790378902 1103 48-49 0.19554043374262875 1103 50-51 0.30272056628746924 1103 52-53 0.04460329771708871 1103 54-55 0.07006368318420186 1103 56-57 0.14534116296150756 1103 58-59 0.19639164552884836 1103 60-61 0.2060061779907585 1103 62-63 0.23152648941692178 1103 64-65 0.13528722909692448 1103 66-67 0.504538301312806 1103 68-69 0.5277716849195073 1103 70-71 0.5398035461036095 1103 72-73 0.30811015660313146 1103 74-75 0.29799315945364313 1103 76-77 0.21725966678839015 1103 78-79 0.29245553322186524 1103 80-81 0.6558955931666652 1103 82-83 0.6421233328300353 1103 84-85 0.4242915979671018 1103 86-87 0.26730917256835696 1103 88-89 0.17825830571155876 1103 90-91 0.31785748754265697 1103 92-93 0.5350733397225191 1103 94-95 0.6868644727518856 1103 96-97 0.7216049121859491 1103 98-99 0.6998229308375876 1103 100-101 1.1770838656669866 1104 1 0.14320340306810664 1104 2 0.1784888839782255 1104 3 0.13125066824260756 1104 4 0.08921406123855036 1104 5 0.08359961259741056 1104 6 0.006634564069550208 1104 7 0.06173619741122849 1104 8 0.008090669082825741 1104 9 0.05936588786721586 1104 10-11 0.04453398588135116 1104 12-13 0.06283570753352308 1104 14-15 0.09724668614671828 1104 16-17 0.2577181811084799 1104 18-19 0.17382779827559602 1104 20-21 0.032627176528805535 1104 22-23 0.16500985370020516 1104 24-25 0.19143297557636885 1104 26-27 0.2087124278571153 1104 28-29 0.09953622881276658 1104 30-31 -0.04311203668005703 1104 32-33 0.0727013598269437 1104 34-35 0.01847272348967266 1104 36-37 0.059395649071788625 1104 38-39 -0.011465593980467759 1104 40-41 -0.01833651404629677 1104 42-43 0.1333360352655788 1104 44-45 0.3028446440013184 1104 46-47 0.11190791616158435 1104 48-49 -0.14867286133150515 1104 50-51 -0.034828364757395036 1104 52-53 -0.03920827569276497 1104 54-55 -0.038718221628315064 1104 56-57 0.02296926930792864 1104 58-59 -0.18672736539982537 1104 60-61 0.0207545705727199 1104 62-63 0.02462123408986372 1104 64-65 0.20684611462042568 1104 66-67 -0.07098820558624652 1104 68-69 0.1555592046247689 1104 70-71 0.053167699352986375 1104 72-73 -0.14507102553548634 1104 74-75 0.028106342938421847 1104 76-77 -0.03224880868851798 1104 78-79 0.12417259222252142 1104 80-81 0.2001992224774689 1104 82-83 0.2795642170428003 1104 84-85 0.1648950660932904 1104 86-87 0.21764305499214842 1104 88-89 0.13734663842495465 1104 90-91 0.3488146761995097 1104 92-93 0.40694485255529145 1104 94-95 0.3449006772969412 1104 96-97 0.3578457680585636 1104 98-99 0.5349895177975768 1104 100-101 1.1875032999266892 1105 1 0.04901160193000109 1105 2 0.15420503505909977 1105 3 0.07297738359303452 1105 4 -0.0018385505433471394 1105 5 -0.058911631417871035 1105 6 0.007123013765678365 1105 7 0.019559721449510903 1105 8 -0.007515540515292685 1105 9 -0.06423499123476972 1105 10-11 -0.022759766368793066 1105 12-13 -0.10322912892448954 1105 14-15 0.0012644401378736347 1105 16-17 0.018634682540508152 1105 18-19 0.0038698726553576535 1105 20-21 0.10689409897331359 1105 22-23 0.21077850371982265 1105 24-25 0.06188150028633288 1105 26-27 0.11983312168137417 1105 28-29 0.003287995345630179 1105 30-31 0.005968828880753563 1105 32-33 -0.045195297328653794 1105 34-35 0.1497853581626174 1105 36-37 0.05311135952697299 1105 38-39 0.16408532246722274 1105 40-41 0.12716561116663172 1105 42-43 0.1243273893298138 1105 44-45 0.3150421840203492 1105 46-47 0.1167499625992292 1105 48-49 0.10274466374922753 1105 50-51 0.26827750899797564 1105 52-53 0.029820305868661023 1105 54-55 0.16904861215370204 1105 56-57 0.014547139151396493 1105 58-59 0.08607447663049328 1105 60-61 0.0879013580353103 1105 62-63 0.045555521753954054 1105 64-65 -0.09595884403061206 1105 66-67 0.21103426632386402 1105 68-69 0.21053424479156035 1105 70-71 0.050072572565632356 1105 72-73 0.21295051138840648 1105 74-75 -0.09324573310712125 1105 76-77 -0.1329839013005163 1105 78-79 0.024428638735397357 1105 80-81 0.07312895342712622 1105 82-83 0.13957259967785163 1105 84-85 -0.03928416109224386 1105 86-87 0.198014255267271 1105 88-89 0.4327326963833542 1105 90-91 0.5758666754042352 1105 92-93 0.6713882402145508 1105 94-95 0.690358471266812 1105 96-97 0.592760249060607 1105 98-99 0.6464264411555725 1105 100-101 0.5501820354384357 1106 1 0.0867819785357895 1106 2 0.15387198231965016 1106 3 0.10205971959810967 1106 4 0.07699674084729935 1106 5 -0.02041293068955241 1106 6 0.0511225745752526 1106 7 0.008601310342868373 1106 8 -0.2060717019909717 1106 9 0.022773511979856664 1106 10-11 0.05718417585326563 1106 12-13 0.16240330528844282 1106 14-15 0.12389708174627856 1106 16-17 0.028555506266549457 1106 18-19 0.1372190656429524 1106 20-21 0.08570773583602431 1106 22-23 0.09382880322328901 1106 24-25 0.06200380262014704 1106 26-27 -0.004939963265378822 1106 28-29 -0.0051685553015659025 1106 30-31 0.16356011339075138 1106 32-33 0.12423094917917155 1106 34-35 0.2493592824510671 1106 36-37 0.1766641660757884 1106 38-39 0.3163078090170188 1106 40-41 0.3654517315292054 1106 42-43 0.2906966874791195 1106 44-45 0.3054477031800289 1106 46-47 0.1964541800472901 1106 48-49 0.18314182609109508 1106 50-51 0.3509069176768591 1106 52-53 0.20905849347617078 1106 54-55 0.12714618562660007 1106 56-57 0.2533779776391256 1106 58-59 0.011998911477810736 1106 60-61 0.0700064831435796 1106 62-63 0.09483665242530037 1106 64-65 0.03477899588447286 1106 66-67 -0.013984948257260044 1106 68-69 0.24633680429576543 1106 70-71 0.2475389927976721 1106 72-73 0.3497345394965947 1106 74-75 0.1319705071788384 1106 76-77 -0.004291041804851403 1106 78-79 0.06201754429725881 1106 80-81 0.1721041757672772 1106 82-83 0.4379512550231617 1106 84-85 0.14694996400383076 1106 86-87 0.03347369725143423 1106 88-89 0.4966190436609814 1106 90-91 0.5941080227428621 1106 92-93 0.3352532379575983 1106 94-95 0.614098238739885 1106 96-97 0.4398917865930905 1106 98-99 0.8962153857565802 1106 100-101 0.967360785941672 1107 1 -0.14041367771018187 1107 2 -0.045660704088845705 1107 3 -0.14708398480475537 1107 4 -0.06262627015074429 1107 5 -0.2876854102734683 1107 6 -0.13778304559440357 1107 7 -0.10132259611656735 1107 8 -0.2051710103863158 1107 9 -0.22698991203485264 1107 10-11 -0.15018562221179366 1107 12-13 -0.03223031209824967 1107 14-15 -0.11349110721285172 1107 16-17 -0.0892483874457568 1107 18-19 -0.09923407041804211 1107 20-21 -0.18336947186188013 1107 22-23 -0.2628700069637091 1107 24-25 -0.09204213325985933 1107 26-27 -0.2184589124544445 1107 28-29 -0.21225129972894763 1107 30-31 -0.2900296383078569 1107 32-33 -0.12989179787549432 1107 34-35 -0.16196638233353156 1107 36-37 -0.12236058135350447 1107 38-39 -0.21618672358425073 1107 40-41 -0.08650661893409506 1107 42-43 -0.37644181346841066 1107 44-45 -0.3123361211042308 1107 46-47 -0.21680909679162141 1107 48-49 -0.33964092782365896 1107 50-51 -0.2713627278605486 1107 52-53 -0.3308890949083718 1107 54-55 -0.2581929147278359 1107 56-57 -0.4728205929310789 1107 58-59 -0.40168065395216956 1107 60-61 -0.25130840496632345 1107 62-63 -0.22960845829712184 1107 64-65 -0.2988874088660154 1107 66-67 -0.09339298527257966 1107 68-69 -0.3563894754647663 1107 70-71 -0.6006184512572759 1107 72-73 -0.4660007435309943 1107 74-75 -0.6311926049691259 1107 76-77 -0.5889186505264199 1107 78-79 -0.5940803385605307 1107 80-81 -0.37939640928695795 1107 82-83 -0.5918536701361141 1107 84-85 -0.3631154623316384 1107 86-87 -0.22393040129067288 1107 88-89 -0.29979059647592976 1107 90-91 -0.30494549086664335 1107 92-93 -0.6093618304122508 1107 94-95 -0.36651134885161696 1107 96-97 -0.18255757155010954 1107 98-99 0.04327746475471628 1107 100-101 0.3740729149309274 1108 1 0.009812905413806305 1108 2 -0.009233374389271631 1108 3 -0.00958161380439293 1108 4 -0.05421587017628582 1108 5 -0.08016293819806464 1108 6 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-1.89995282911784 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 15.0 5 29.0 6 94.0 7 220.0 8 491.0 9 974.0 10 1589.0 11 2608.0 12 3746.0 13 5290.0 14 6760.0 15 9070.0 16 11395.0 17 15197.0 18 13021.0 19 8479.0 20 7548.0 21 7626.0 22 8293.0 23 9002.0 24 10491.0 25 12506.0 26 15444.0 27 19047.0 28 23526.0 29 28716.0 30 35540.0 31 43871.0 32 54671.0 33 69298.0 34 90355.0 35 122625.0 36 171186.0 37 222068.0 38 135543.0 39 7639.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74004012073717 19.173246933422412 14.95690514700995 22.129807798830463 2 15.89833231536166 20.679136180757695 39.55411988895165 23.868411614929002 3 19.994275594354306 24.611962815214994 30.322568554442757 25.071193035987942 4 12.970118232307746 17.02562267879655 37.06497666019285 32.93928242870285 5 13.32322513873923 37.770632003509476 33.861316138045 15.044826719706295 6 31.50702317776434 36.22503130403673 17.13723519340358 15.130710324795352 7 28.18546236969473 33.59418522045633 20.240226785261004 17.980125624587938 8 26.950682042362097 33.66573706061634 19.43441677584001 19.94916412118156 9 26.477420344050667 16.704899250823072 20.570124067787948 36.24755633733832 10-11 25.21412478225501 26.6122925300158 26.65535173535838 21.51823095237081 12-13 26.690173957930497 24.047669345345078 27.59156599617364 21.670590700550786 14-15 23.08162120560993 25.203383268587725 26.97048903555379 24.74450649024855 16-17 21.699044198727954 28.599332246676035 27.571763890125233 22.12985966447078 18-19 21.452987885592016 26.525970762555218 30.740459328741522 21.280582023111243 20-21 22.32634623935453 25.285994408734773 30.953326053217534 21.434333298693158 22-23 21.759427848840946 25.571880705870026 30.964759737485792 21.703931707803235 24-25 22.032749796309332 25.561230074410446 31.10204210274357 21.303978026536647 26-27 21.8845021051309 25.727276405709944 31.057889502456522 21.330331986702646 28-29 21.339859690699388 26.1775107301987 31.090508485982486 21.392121093119425 30-31 21.600356060589927 25.7779348911694 30.942261538428777 21.679447509811897 32-33 21.439781868268298 25.873968616731524 30.984109893826034 21.702139621174144 34-35 22.11281751595992 25.69888012212513 30.714094650381046 21.474207711533907 36-37 22.198969873133894 25.4530445479607 30.744414040802976 21.603571538102425 38-39 21.848411986821397 25.846248208387195 30.854458214158694 21.450881590632715 40-41 21.811681638156877 25.91563464822074 30.736609243892538 21.536074469729847 42-43 21.72655693326854 26.03579403671229 30.248563310792942 21.98908571922623 44-45 21.95385184137763 26.069246088872006 29.96197478955806 22.014927280192307 46-47 22.301312593512545 26.51205499953789 29.374768680894412 21.811863726055144 48-49 22.501096710719658 26.619490359593346 28.935913762335336 21.943499167351668 50-51 22.28534191965655 26.83008790759481 29.0018825172919 21.882687655456746 52-53 22.612047412783394 27.01945150752378 28.51658297450051 21.851918105192322 54-55 22.05039789154112 26.78339912329271 28.721746804345717 22.444456180820453 56-57 22.408704222208783 27.036336021956476 28.36513029841758 22.18982945741717 58-59 22.811298278102484 26.62524867617602 28.445700963357982 22.11775208236351 60-61 22.397908573341553 26.566749195334836 29.314387374774846 21.72095485654876 62-63 22.319541567390427 26.792317658974728 29.065186261673563 21.822954511961278 64-65 22.03558907427261 26.97118040603252 28.83220182106815 22.161028698626723 66-67 22.043111735215188 27.051972848917266 28.53589528340018 22.36902013246737 68-69 22.116150036820496 26.831686668424283 28.83673207753217 22.21543121722305 70-71 22.31780458323999 26.873871886321066 28.54912336144017 22.259200168998778 72-73 22.248945559210316 26.77417142866878 28.711965917168637 22.26491709495227 74-75 22.12884474009485 27.299475513008247 28.20959716639024 22.362082580506666 76-77 22.13445014511 27.02474664313332 28.3824430101445 22.45836020161218 78-79 22.25867619136048 26.95247846764418 28.36269763725466 22.42614770374068 80-81 22.35916115345021 27.300718148530372 28.180248849688844 22.159871848330575 82-83 22.291505614723288 27.318242899339605 28.16071759421938 22.229533891717722 84-85 22.07707188838734 27.302228351748887 28.14947734805783 22.471222411805947 86-87 22.146641100361943 27.178367567024235 27.947954795002474 22.727036537611344 88-89 22.010303460114024 27.247263875489562 27.738797276547167 23.003635387849243 90-91 22.057931530352672 26.855330481141287 28.130510787726376 22.956227200779665 92-93 22.097819656693783 26.770780937956918 28.37321167171295 22.75818773363635 94-95 21.866117069622156 26.805846240601184 28.354505426546766 22.973531263229894 96-97 21.696960043067758 26.610739341240446 28.582137101308046 23.110163514383746 98-99 21.689718648552184 26.83533499554066 28.05566889647776 23.41927745942939 100-101 21.33176311244471 26.858401143166237 28.293705132109388 23.516130612279667 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 398.0 1 317.5 2 371.0 3 487.0 4 376.5 5 324.0 6 551.5 7 1092.0 8 1696.5 9 2092.0 10 1939.5 11 1586.5 12 1586.0 13 1678.0 14 1884.5 15 2027.0 16 2124.5 17 2309.5 18 2397.5 19 2537.5 20 2762.0 21 3142.0 22 3626.0 23 4195.5 24 4935.5 25 5849.0 26 7019.5 27 8556.5 28 10094.5 29 11933.5 30 14405.5 31 16879.0 32 19475.5 33 21237.5 34 22891.5 35 24920.0 36 27025.0 37 29616.5 38 31345.5 39 31871.0 40 32633.5 41 34596.0 42 36617.0 43 38289.5 44 39828.0 45 41388.5 46 42491.0 47 45476.5 48 53661.5 49 53094.0 50 44692.0 51 40774.5 52 38155.0 53 36977.5 54 35856.5 55 33313.0 56 30294.0 57 27404.0 58 25388.0 59 22705.5 60 19158.5 61 15828.5 62 13285.5 63 10647.0 64 7643.0 65 5790.0 66 4543.5 67 3816.5 68 2774.5 69 1669.0 70 1218.0 71 939.5 72 793.5 73 703.0 74 593.0 75 513.0 76 387.0 77 231.0 78 141.0 79 98.5 80 76.5 81 52.0 82 33.5 83 18.0 84 11.0 85 7.5 86 2.5 87 1.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043953273241145036 2 0.006047834108762204 3 0.004514580391047842 4 0.0011925306693333923 5 7.666268588571808E-4 6 3.407230483809693E-4 7 0.0 8 0.0 9 0.001618434479809604 10-11 7.666268588571808E-4 12-13 0.0010221691451429077 14-15 0.0017462056229524674 16-17 0.0031942785785715865 18-19 0.0 20-21 0.0 22-23 0.0015758440987619828 24-25 0.0010647595261905288 26-27 0.00481271305838119 28-29 0.005749701441428856 30-31 0.0014054825745714982 32-33 3.833134294285904E-4 34-35 0.008177353161143262 36-37 0.011754945169143438 38-39 0.008645847352667095 40-41 0.0017462056229524674 42-43 6.388557157143174E-4 44-45 0.0013628921935238771 46-47 4.259038104762116E-5 48-49 7.666268588571808E-4 50-51 0.0011925306693333923 52-53 0.0024702421007620273 54-55 0.003066507435428723 56-57 0.0012777114314286347 58-59 6.388557157143174E-4 60-61 0.003279459340666829 62-63 0.005196026487809782 64-65 0.008731028114762337 66-67 0.0020869286713334364 68-69 0.003023917054381102 70-71 8.518076209524232E-5 72-73 8.943980020000444E-4 74-75 0.00553674953619075 76-77 0.0031516881975239656 78-79 0.0036627727700954196 80-81 0.0044719900100002215 82-83 0.004386809247904979 84-85 0.004855303439428812 86-87 0.0028109651491429965 88-89 0.008219943542190883 90-91 0.011925306693333923 92-93 6.388557157143174E-4 94-95 0.0019591575281905733 96-97 8.518076209524232E-4 98-99 0.0028109651491429965 100-101 0.003535001626952556 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1173974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.29007570250047 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02115651144483 50.27082891793092 2 10.846008076111072 13.295053121095583 3 3.094916727434598 5.690630415522045 4 1.3349114691956456 3.2726730001254896 5 0.6930028375315619 2.123709818717853 6 0.42391370546299445 1.5589021859492649 7 0.2790503707741813 1.1972112844692278 8 0.19700198230506805 0.9659413127216222 9 0.1463444701913586 0.8072517291003621 >10 0.8489513666674683 9.644245017399044 >50 0.07382941964622097 3.1075594019228667 >100 0.03618162598659455 4.256887921113497 >500 0.0032006781386908923 1.280988829390661 >1k 0.0012524392716616536 1.4062816312000281 >5k 2.7831983814703416E-4 1.1218354133414585 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6925 0.589876777509553 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6228 0.5305057863291691 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3905 0.33263087598192126 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3216 0.2739413308982993 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1795 0.15289946796095996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.14421103022724524 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1390 0.1184012593123868 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1291 0.10996836386495783 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 8.518076209524231E-5 0.0 24-25 0.0 0.0 0.0 1.7036152419048462E-4 0.0 26-27 0.0 0.0 0.0 3.407230483809693E-4 8.518076209524231E-5 28-29 0.0 0.0 0.0 0.0010647595261905288 8.518076209524231E-5 30-31 0.0 0.0 0.0 0.0018739767660953311 8.518076209524231E-5 32-33 0.0 0.0 0.0 0.002981326673333481 8.518076209524231E-5 34-35 0.0 0.0 0.0 0.004344218866857358 8.518076209524231E-5 36-37 0.0 0.0 0.0 0.0066440994434289 8.518076209524231E-5 38-39 0.0 0.0 0.0 0.009753197259905245 8.518076209524231E-5 40-41 0.0 0.0 0.0 0.01256416240904824 8.518076209524231E-5 42-43 0.0 0.0 0.0 0.019421213757715248 8.518076209524231E-5 44-45 0.0 0.0 0.0 0.027598566918858508 8.518076209524231E-5 46-47 0.0 0.0 0.0 0.03675549884409706 8.518076209524231E-5 48-49 0.0 0.0 0.0 0.04808454020276429 8.518076209524231E-5 50-51 0.0 0.0 0.0 0.0612023775654316 8.518076209524231E-5 52-53 0.0 0.0 0.0 0.08803431762543293 8.518076209524231E-5 54-55 0.0 0.0 0.0 0.11201270215524364 8.518076209524231E-5 56-57 0.0 0.0 0.0 0.15494380625124576 8.518076209524231E-5 58-59 0.0 0.0 0.0 0.20405051559915297 8.518076209524231E-5 60-61 0.0 0.0 0.0 0.25277391151763157 8.518076209524231E-5 62-63 0.0 0.0 0.0 0.32504978815544466 8.518076209524231E-5 64-65 0.0 0.0 0.0 0.3635940830035418 8.518076209524231E-5 66-67 0.0 0.0 0.0 0.4623185862719276 8.518076209524231E-5 68-69 0.0 0.0 0.0 0.5840844856870766 8.518076209524231E-5 70-71 0.0 0.0 0.0 0.6619397022421281 8.518076209524231E-5 72-73 0.0 0.0 0.0 0.8042341653222302 8.518076209524231E-5 74-75 0.0 0.0 0.0 0.943334349823761 8.518076209524231E-5 76-77 0.0 0.0 0.0 1.0241708930521458 8.518076209524231E-5 78-79 0.0 0.0 0.0 1.1105441858167215 8.518076209524231E-5 80-81 0.0 0.0 0.0 1.2862720980192064 8.518076209524231E-5 82-83 0.0 0.0 0.0 1.4291628264339755 8.518076209524231E-5 84-85 0.0 0.0 0.0 1.6644746817220826 8.518076209524231E-5 86-87 0.0 0.0 0.0 2.00289784952648 8.518076209524231E-5 88-89 0.0 0.0 0.0 2.451715285006312 8.518076209524231E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 5705 0.0 24.820793 1 TACATGG 5850 0.0 24.198362 2 GAGTACT 6650 0.0 23.464012 12-13 GGTATCA 8510 0.0 23.451725 1 ACATGGG 5610 0.0 23.285034 3 GTATCAA 11005 0.0 22.279976 1 GTACTTT 7045 0.0 21.979872 14-15 ATGGGCG 160 4.989851E-5 20.781008 5 GTACACG 115 0.003715313 20.659853 1 TATTCCG 115 0.0037235762 20.651932 5 GTAAGGT 490 0.0 20.356907 4 TAAGGTG 565 0.0 20.176756 5 AGTACTT 6800 0.0 19.907856 12-13 TAGACCG 120 0.0047638807 19.791435 5 CATGGGA 3460 0.0 19.631273 4 TCAACGC 13050 0.0 18.890583 4 TATCAAC 13035 0.0 18.877491 2 ACTTTTT 8305 0.0 18.845354 16-17 GTGTAAG 530 0.0 18.827753 1 ATCAACG 13090 0.0 18.761086 3 >>END_MODULE