FastQCFastQC Report
Thu 26 May 2016
SRR936444_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936444_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275335
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.3906424586481199No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41400.3246205898842265No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT32510.2549134149066716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.18418689991257198No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18320.1436485315622954No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA13630.10687388019618375No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT13170.10326698475302569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG63750.024.1574251
GTATAGC4400.023.7659851
TACATGG64250.023.4497322
GAGTACT48300.023.18189612-13
ACATGGG64350.022.1602973
GTACTTT53250.020.97819714-15
AGTACTT50400.020.94245512-13
GTACACG2056.786413E-720.8676931
GTATCAA114000.020.7222711
TACCTGG9650.020.6858652
TCGCGCT2000.020.179760-61
GTACCTG10750.019.8971021
CTCGCGC1950.019.4796560-61
GGTATCA89600.019.2568131
TATCAAC129250.018.1655332
TCAACGC129650.018.1482834
TAAGGTG4201.8189894E-1218.1088395
CATGGGA39650.018.1024194
CATGGGG26850.018.0575474
ATCAACG129950.018.0332283
Produced by FastQC (version 0.11.5)