##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936444_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275335 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.30772855759467 31.0 31.0 34.0 28.0 34.0 2 31.348643297643363 31.0 31.0 34.0 28.0 34.0 3 31.279470100012936 31.0 31.0 34.0 27.0 34.0 4 34.9499417800029 37.0 35.0 37.0 32.0 37.0 5 34.98769813421572 37.0 35.0 37.0 32.0 37.0 6 35.05364394453222 37.0 35.0 37.0 32.0 37.0 7 34.90989269486057 37.0 35.0 37.0 32.0 37.0 8 34.899924333606464 37.0 35.0 37.0 32.0 37.0 9 36.37472899277445 39.0 37.0 39.0 32.0 39.0 10-11 36.25830076019242 39.0 36.0 39.0 32.0 39.0 12-13 36.162427519044016 39.0 35.0 39.0 31.5 39.0 14-15 37.13322342757001 39.5 36.5 41.0 31.0 41.0 16-17 37.04973399146107 39.0 36.0 41.0 31.0 41.0 18-19 36.93835266812249 39.0 36.0 41.0 31.0 41.0 20-21 36.951604480391424 39.0 36.0 41.0 31.0 41.0 22-23 36.963217507556834 39.0 36.0 41.0 31.0 41.0 24-25 37.07709739009751 39.0 36.0 41.0 31.0 41.0 26-27 37.01378186907753 39.0 36.0 41.0 31.0 41.0 28-29 36.90193164933135 39.0 36.0 41.0 30.0 41.0 30-31 36.708556967385036 39.0 36.0 41.0 30.0 41.0 32-33 36.538783927360264 39.0 35.0 41.0 30.0 41.0 34-35 36.355797888397944 39.0 35.0 40.0 30.0 41.0 36-37 36.14741538497728 39.0 35.0 40.0 29.5 41.0 38-39 35.94941054703274 38.0 35.0 40.0 29.0 41.0 40-41 35.73376759831731 38.0 35.0 40.0 28.0 41.0 42-43 35.45397209360678 38.0 35.0 40.0 27.0 41.0 44-45 35.27807791678265 38.0 34.0 40.0 26.5 41.0 46-47 35.10049241963876 38.0 34.0 40.0 25.5 41.0 48-49 34.84991355212552 38.0 34.0 40.0 24.0 41.0 50-51 33.86278899269604 37.5 33.0 39.5 23.5 40.5 52-53 33.95274261272529 37.5 33.0 39.5 23.5 40.5 54-55 34.39638212704897 38.0 34.0 40.0 23.5 41.0 56-57 34.25018916598384 38.0 34.0 40.0 23.0 41.0 58-59 34.026785903311676 38.0 33.0 40.0 22.5 41.0 60-61 33.735133121885625 37.0 33.0 40.0 22.0 41.0 62-63 33.41352585791184 37.0 33.0 40.0 21.5 41.0 64-65 33.04440833192848 36.0 32.0 39.5 20.0 41.0 66-67 32.694428522701884 36.0 32.0 39.0 19.5 41.0 68-69 32.27304276915477 35.0 31.5 39.0 18.0 40.0 70-71 31.87141496155912 35.0 31.0 38.0 16.0 40.0 72-73 31.459768609816244 35.0 31.0 37.5 13.0 40.0 74-75 31.05467347794893 35.0 30.5 37.0 9.5 39.0 76-77 30.591673168226386 34.5 30.0 36.5 7.5 39.0 78-79 30.12579283090325 34.0 30.0 36.0 6.5 38.5 80-81 29.675538191926044 34.0 29.0 35.5 2.0 37.0 82-83 29.294076458342317 34.0 29.0 35.0 2.0 37.0 84-85 28.93423688677877 34.0 29.0 35.0 2.0 36.5 86-87 28.58172088118024 34.0 29.0 35.0 2.0 36.0 88-89 28.22173350531429 34.0 27.5 35.0 2.0 36.0 90-91 27.877856406355978 34.0 27.0 35.0 2.0 35.0 92-93 27.457628387835353 33.5 26.5 35.0 2.0 35.0 94-95 26.97684922000886 33.0 25.5 35.0 2.0 35.0 96-97 26.39076713177322 33.0 25.0 35.0 2.0 35.0 98-99 25.65429671419666 33.0 22.0 35.0 2.0 35.0 100-101 23.09743518369683 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4352375211045789 1101 2 0.41252697422180873 1101 3 0.6698335337726 1101 4 0.6361044017840314 1101 5 0.4698528960955244 1101 6 0.37329228787789503 1101 7 0.5949158206543927 1101 8 0.5452869995873257 1101 9 0.6109438806800469 1101 10-11 0.74009441255577 1101 12-13 0.7079722644830824 1101 14-15 0.9267735147538474 1101 16-17 0.9583266921602842 1101 18-19 1.0916590274272764 1101 20-21 0.9523655690771591 1101 22-23 0.8901641048900117 1101 24-25 0.9693571424922638 1101 26-27 0.8262939123858715 1101 28-29 0.7855838838398981 1101 30-31 0.6964129990483698 1101 32-33 0.49090429118327705 1101 34-35 0.6991771227109425 1101 36-37 0.6201601286028975 1101 38-39 0.6794478497599457 1101 40-41 0.7049689755825526 1101 42-43 0.6292246918780293 1101 44-45 0.7291077201903491 1101 46-47 0.7934973028709393 1101 48-49 0.9558874934428374 1101 50-51 0.7650997925501244 1101 52-53 0.8754924001029138 1101 54-55 0.8705697802066723 1101 56-57 0.8779940597329912 1101 58-59 0.8282955724868302 1101 60-61 0.8076461411611646 1101 62-63 0.8414032882042619 1101 64-65 0.6972436087849161 1101 66-67 0.7197760099718025 1101 68-69 0.8206358990912364 1101 70-71 0.6008841567103786 1101 72-73 0.8879668346052014 1101 74-75 0.7262764665985877 1101 76-77 0.7988242631076439 1101 78-79 0.8976970107724433 1101 80-81 0.8265496168178643 1101 82-83 0.7587396862513636 1101 84-85 0.7986375023552661 1101 86-87 0.8597414356520581 1101 88-89 1.0005467794521046 1101 90-91 0.8185220119246637 1101 92-93 1.0352938270648195 1101 94-95 1.008570958885631 1101 96-97 1.0304147271892354 1101 98-99 0.9594618611818007 1101 100-101 1.4268436709039705 1102 1 0.32072836095961677 1102 2 0.35271415056317323 1102 3 0.41370654038375676 1102 4 0.42452023938019323 1102 5 0.29559840637802637 1102 6 0.2974191375696549 1102 7 0.4874625140429103 1102 8 0.33318084663049063 1102 9 0.48701113091654946 1102 10-11 0.44986439924201704 1102 12-13 0.530870776238487 1102 14-15 0.6751874037304333 1102 16-17 0.40987165356346367 1102 18-19 0.4568164573831055 1102 20-21 0.6210245813338631 1102 22-23 0.349690241974848 1102 24-25 0.5832068745298571 1102 26-27 0.4458937051137113 1102 28-29 0.3345595522218616 1102 30-31 0.03441385687643361 1102 32-33 0.2834323692674019 1102 34-35 -0.03824366699164017 1102 36-37 0.1289076887461178 1102 38-39 -0.06923247756459716 1102 40-41 0.17729150151606632 1102 42-43 0.10955016577904786 1102 44-45 0.416154788606498 1102 46-47 0.47338292216727496 1102 48-49 0.6645930875051036 1102 50-51 0.47925199606874 1102 52-53 0.21832861046238605 1102 54-55 0.4349917967775596 1102 56-57 0.44879453959417503 1102 58-59 0.11252160582673554 1102 60-61 0.17741711848641728 1102 62-63 0.3579948586283592 1102 64-65 0.3700213932153389 1102 66-67 0.3700935043300504 1102 68-69 0.4165586031220485 1102 70-71 0.2320730650370848 1102 72-73 0.3411790087965514 1102 74-75 0.028005512678543454 1102 76-77 0.11750766032449533 1102 78-79 0.06867259424248928 1102 80-81 0.3413411635101653 1102 82-83 0.1937671030023047 1102 84-85 0.21694761373139215 1102 86-87 -0.10029934383932115 1102 88-89 0.40520376253177304 1102 90-91 0.744153598721514 1102 92-93 0.3927174689368442 1102 94-95 0.4893321506570132 1102 96-97 0.5515142493964227 1102 98-99 0.4951077197120206 1102 100-101 0.9323038880389802 1103 1 0.3867196723278852 1103 2 0.3410460101610333 1103 3 0.43786939456083473 1103 4 0.5312058723816193 1103 5 0.4991853503632626 1103 6 0.49339391747046335 1103 7 0.5554914248883236 1103 8 0.4611267156858858 1103 9 0.33884304818231925 1103 10-11 0.4204249313245967 1103 12-13 0.46524469496517895 1103 14-15 0.7250989796021727 1103 16-17 0.7350129399423011 1103 18-19 0.717696469685599 1103 20-21 0.653414723965831 1103 22-23 0.47765533366682433 1103 24-25 0.5000209147436223 1103 26-27 0.5036385053905192 1103 28-29 0.4598911427028085 1103 30-31 0.5518680108881995 1103 32-33 0.7657090983435637 1103 34-35 0.5539961546222969 1103 36-37 0.41188412179366907 1103 38-39 0.301533736729354 1103 40-41 0.5647237417221334 1103 42-43 0.7438971728145347 1103 44-45 0.6032006538173675 1103 46-47 0.8734156006494587 1103 48-49 0.9521060627847859 1103 50-51 0.7745213052071804 1103 52-53 0.7809751830662819 1103 54-55 0.800510231286033 1103 56-57 0.7573274679706259 1103 58-59 0.7594306058651767 1103 60-61 0.585802032561233 1103 62-63 0.6294857663212525 1103 64-65 0.8104119971905774 1103 66-67 0.8324075253211731 1103 68-69 0.9837032726849273 1103 70-71 0.6777306714343752 1103 72-73 0.7759373802547813 1103 74-75 0.8086560067002999 1103 76-77 0.7433678733113069 1103 78-79 0.7904633750659862 1103 80-81 0.8463582716567153 1103 82-83 0.6541973056088963 1103 84-85 0.799431947282585 1103 86-87 0.7887379865209105 1103 88-89 0.5568511424864049 1103 90-91 0.8273107245528912 1103 92-93 0.8785984423711604 1103 94-95 0.8907392476544373 1103 96-97 0.9358900903867706 1103 98-99 1.2992002190392284 1103 100-101 1.5388741852209407 1104 1 0.13308312145467127 1104 2 0.28519798208880687 1104 3 0.5053031840229707 1104 4 0.4453665254497565 1104 5 0.33911070410530186 1104 6 0.4032034135986038 1104 7 0.32966532102646795 1104 8 0.40365987629468236 1104 9 0.5038097383964981 1104 10-11 0.38511809824694865 1104 12-13 0.509293782867978 1104 14-15 0.61339185548497 1104 16-17 0.6682687333042097 1104 18-19 0.6376288299355934 1104 20-21 0.6797401377636518 1104 22-23 0.5137022772702124 1104 24-25 0.5929976604516511 1104 26-27 0.328525852676556 1104 28-29 0.3089509424783756 1104 30-31 0.3166418296467697 1104 32-33 0.35815602500813526 1104 34-35 0.37427730037882867 1104 36-37 0.37902234826172077 1104 38-39 0.41465563649394 1104 40-41 0.5791958379191797 1104 42-43 0.6239728007535561 1104 44-45 0.688053026309511 1104 46-47 0.9727265952848612 1104 48-49 0.8769054437482637 1104 50-51 0.7808045452166539 1104 52-53 0.6534735806163141 1104 54-55 0.7394328266967776 1104 56-57 0.8932027136314957 1104 58-59 0.7840626198319924 1104 60-61 0.6882264960532822 1104 62-63 0.7008626413802048 1104 64-65 0.9072360664703112 1104 66-67 0.8327342711254531 1104 68-69 0.6998338324163669 1104 70-71 0.7226522043197292 1104 72-73 0.9470646003457546 1104 74-75 0.7912321302920198 1104 76-77 0.7226640272388956 1104 78-79 0.5122015472528432 1104 80-81 0.737702058289301 1104 82-83 0.6715634360723861 1104 84-85 0.6288547573157501 1104 86-87 0.6628855120109343 1104 88-89 0.6477716513436711 1104 90-91 0.7489658388224925 1104 92-93 0.8353756026950769 1104 94-95 0.8223468755087708 1104 96-97 0.5317401780643678 1104 98-99 0.3727252432443997 1104 100-101 1.0400247731709023 1105 1 0.2797160858122467 1105 2 0.1947629356581686 1105 3 0.3366163642192639 1105 4 0.2990323030256832 1105 5 0.27860289865795806 1105 6 0.1709264416691738 1105 7 0.2864538359347151 1105 8 0.419890590688091 1105 9 0.14962744439348796 1105 10-11 0.33780984682722703 1105 12-13 0.4027066628332818 1105 14-15 0.5891048808193986 1105 16-17 0.7754082001848062 1105 18-19 0.33296929336716374 1105 20-21 0.3890446926009403 1105 22-23 0.3347288699555975 1105 24-25 0.12733390426974012 1105 26-27 0.15915592826478075 1105 28-29 0.19884592677813373 1105 30-31 -0.1452499606270834 1105 32-33 0.016459468637116004 1105 34-35 -0.1071108910319154 1105 36-37 0.17228315834560703 1105 38-39 -0.128569808501247 1105 40-41 -0.3368627973978917 1105 42-43 -0.4268952260420136 1105 44-45 -0.2226077796794712 1105 46-47 0.0788404745931146 1105 48-49 0.19114938468484155 1105 50-51 0.06568894790961366 1105 52-53 0.2561254038727725 1105 54-55 0.09004603872164552 1105 56-57 0.18617827635520712 1105 58-59 0.25938755985094275 1105 60-61 -0.10877231084030115 1105 62-63 0.10460957252810488 1105 64-65 0.08716851915089308 1105 66-67 0.07999254609087103 1105 68-69 0.1185913823974758 1105 70-71 0.4057884548385715 1105 72-73 0.24334959084371022 1105 74-75 0.21248338435668046 1105 76-77 0.14634802714210693 1105 78-79 0.31200350275611655 1105 80-81 0.34081316236875736 1105 82-83 0.19015197815590668 1105 84-85 0.3296414444073079 1105 86-87 0.4074862866925102 1105 88-89 0.17887338553594034 1105 90-91 0.07694679006892358 1105 92-93 0.30150841163239406 1105 94-95 0.2550978326928188 1105 96-97 0.4968329741957973 1105 98-99 0.6898100245299439 1105 100-101 0.763519104519446 1106 1 0.36090816193915387 1106 2 0.1814042717876312 1106 3 0.22832831767722084 1106 4 0.39411483397478975 1106 5 0.3224982016096831 1106 6 0.2382947717328392 1106 7 0.38403653600461496 1106 8 0.46679718339259324 1106 9 0.5276105473467112 1106 10-11 0.6958817945726565 1106 12-13 0.5763279525109084 1106 14-15 0.4327968629903083 1106 16-17 0.24912400959694736 1106 18-19 0.45147646408896946 1106 20-21 0.3644681522862996 1106 22-23 0.03342043872011402 1106 24-25 0.012793555805735934 1106 26-27 -0.02215988791218848 1106 28-29 0.10482134037243895 1106 30-31 0.06905333685264026 1106 32-33 0.1135756771743317 1106 34-35 0.13093320815554677 1106 36-37 0.15943608488704797 1106 38-39 0.2236294195562678 1106 40-41 0.33425362535900405 1106 42-43 0.45948425471956256 1106 44-45 0.19259505253014453 1106 46-47 0.40908970227480523 1106 48-49 0.15692450288148763 1106 50-51 0.0731288536529533 1106 52-53 0.3122161954332654 1106 54-55 0.29558840911874285 1106 56-57 0.4282610741393498 1106 58-59 0.21646000368405538 1106 60-61 0.44848071642196885 1106 62-63 0.13977636321668285 1106 64-65 0.12586382736414947 1106 66-67 0.05416498165585182 1106 68-69 0.19725040480410172 1106 70-71 -0.020917880050674853 1106 72-73 0.04836176754111676 1106 74-75 0.24310458383654066 1106 76-77 0.009051535834917246 1106 78-79 0.10944564465222228 1106 80-81 0.2774313853225827 1106 82-83 0.330585190473915 1106 84-85 0.48980118839600095 1106 86-87 0.536436666650566 1106 88-89 0.464344593659753 1106 90-91 0.7104122255361887 1106 92-93 0.7413295945759977 1106 94-95 0.7322420964911949 1106 96-97 0.8838983337466111 1106 98-99 0.9085738830397254 1106 100-101 1.4730123246545936 1107 1 0.12369676377590721 1107 2 0.13054669160853294 1107 3 0.29973849706096445 1107 4 0.18599965072871782 1107 5 0.29610561728215146 1107 6 0.2667651462357412 1107 7 0.39651400574970097 1107 8 0.38020947990505505 1107 9 0.2774531931854085 1107 10-11 0.3852628920464056 1107 12-13 0.30090273865229733 1107 14-15 0.032639866452107924 1107 16-17 0.2520100279644666 1107 18-19 0.43948714986701276 1107 20-21 0.29365699158478975 1107 22-23 0.27579233812789283 1107 24-25 0.22178133940084166 1107 26-27 0.07512253419750436 1107 28-29 0.12651296913106336 1107 30-31 -0.04781789022371896 1107 32-33 0.03374883827638797 1107 34-35 -0.08945738778756862 1107 36-37 0.047296211342576555 1107 38-39 0.07070084404058008 1107 40-41 0.1955369915936629 1107 42-43 0.037424631797350116 1107 44-45 0.07303449978788734 1107 46-47 0.05533787839703308 1107 48-49 -0.11053823279213049 1107 50-51 -0.10027900833313907 1107 52-53 0.09242461817237313 1107 54-55 0.24538885543235978 1107 56-57 0.4339061546760661 1107 58-59 0.4558797244519397 1107 60-61 0.30847663951869464 1107 62-63 0.3524387241013045 1107 64-65 0.4483003850273022 1107 66-67 0.40132402379816057 1107 68-69 0.30534556115691913 1107 70-71 0.3562689851683345 1107 72-73 0.5278914216480644 1107 74-75 0.20629086255367568 1107 76-77 0.22834065264892445 1107 78-79 0.44267705540380575 1107 80-81 0.4115901127565671 1107 82-83 0.47778106779488994 1107 84-85 0.5024860795355295 1107 86-87 0.5075496612433064 1107 88-89 0.5467928098357611 1107 90-91 0.27996455294728406 1107 92-93 0.14283483017809218 1107 94-95 0.06765679623084608 1107 96-97 0.36097648154508377 1107 98-99 0.7563677061736485 1107 100-101 0.9740333385875886 1108 1 0.24058416327928 1108 2 0.14645412217869236 1108 3 0.32736192481223725 1108 4 0.22700771628627336 1108 5 0.2698505855327866 1108 6 0.26416440978318434 1108 7 0.4115322325815214 1108 8 0.5845492369052252 1108 9 0.032442511671625596 1108 10-11 0.24090310951391558 1108 12-13 0.24545825669648735 1108 14-15 0.5617421488646883 1108 16-17 0.5186641498480995 1108 18-19 0.6064148587099822 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2314 3 -0.4048241059009854 2314 4 -0.2282830843598802 2314 5 -0.3169822351987861 2314 6 -0.10237886006877517 2314 7 -0.29653415005242323 2314 8 -0.23404108284881886 2314 9 -0.37921874122075394 2314 10-11 -0.2600326637699695 2314 12-13 -0.8492726372505217 2314 14-15 -0.9842608268774313 2314 16-17 -1.2839663971546926 2314 18-19 -1.3847146199257807 2314 20-21 -1.4801777830411922 2314 22-23 -1.0845116118383658 2314 24-25 -0.9240392437672611 2314 26-27 -0.9770328026374102 2314 28-29 -0.6869700162100898 2314 30-31 -1.0699261527499715 2314 32-33 -0.9804593869425346 2314 34-35 -1.431514017384444 2314 36-37 -1.0376316786133728 2314 38-39 -0.9621992980626359 2314 40-41 -1.016443325644424 2314 42-43 -1.0005709479124931 2314 44-45 -1.1131741529982122 2314 46-47 -1.3664676288359843 2314 48-49 -1.5759385635279273 2314 50-51 -1.1577189393355383 2314 52-53 -1.197955329765037 2314 54-55 -1.3785189095230521 2314 56-57 -1.5050858766453246 2314 58-59 -1.5135573435270473 2314 60-61 -1.8029499362969261 2314 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-1.5380133962718432 2315 84-85 -1.4003913904864227 2315 86-87 -1.5749074002107655 2315 88-89 -1.7111711981534903 2315 90-91 -1.5259294463037278 2315 92-93 -1.5395259453959547 2315 94-95 -1.779060488599125 2315 96-97 -1.7881307858818332 2315 98-99 -1.8074948904793864 2315 100-101 -2.2154111956053697 2316 1 -4.9032084136528304 2316 2 -5.176734928672534 2316 3 -6.107689322050515 2316 4 -8.027668791756206 2316 5 -8.401557497748005 2316 6 -9.427461148121441 2316 7 -11.317789751024431 2316 8 -11.529212307547873 2316 9 -11.62709657565204 2316 10-11 -11.274740628972545 2316 12-13 -9.748946469883883 2316 14-15 -9.80630413403778 2316 16-17 -8.50095987497788 2316 18-19 -9.368165708281843 2316 20-21 -10.709326306538358 2316 22-23 -10.382527217305196 2316 24-25 -10.193346795071463 2316 26-27 -8.136356753025794 2316 28-29 -6.406485101962907 2316 30-31 -8.374390484449247 2316 32-33 -5.865069706777348 2316 34-35 -4.832235471740912 2316 36-37 -5.557651203004813 2316 38-39 -4.269234399828257 2316 40-41 -3.5508424823967246 2316 42-43 -3.795003570033579 2316 44-45 -4.329676302085904 2316 46-47 -4.37632756356739 2316 48-49 -4.903364539396339 2316 50-51 -4.760971424364492 2316 52-53 -4.865589144419097 2316 54-55 -4.536971987775129 2316 56-57 -5.165809484771593 2316 58-59 -5.259417189298404 2316 60-61 -5.567864792158225 2316 62-63 -5.094267592300522 2316 64-65 -5.712604440299543 2316 66-67 -5.353867565559177 2316 68-69 -5.529178029670064 2316 70-71 -5.139896165929308 2316 72-73 -4.044168910246249 2316 74-75 -4.305596403604742 2316 76-77 -4.953565902569839 2316 78-79 -5.194294409033905 2316 80-81 -5.66298659953026 2316 82-83 -4.907077785271458 2316 84-85 -4.8527234976360205 2316 86-87 -5.513300894465338 2316 88-89 -7.019735312389525 2316 90-91 -6.4351754458280155 2316 92-93 -5.634766896652071 2316 94-95 -5.053408847809447 2316 96-97 -7.071666808617493 2316 98-99 -8.876801440515063 2316 100-101 -8.888895689917355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3058.0 3 1154.0 4 1172.0 5 1293.0 6 1399.0 7 1683.0 8 2091.0 9 2765.0 10 3351.0 11 4319.0 12 5383.0 13 6619.0 14 7564.0 15 8944.0 16 11013.0 17 13786.0 18 11119.0 19 8655.0 20 8862.0 21 9282.0 22 10172.0 23 11566.0 24 13132.0 25 15349.0 26 17786.0 27 21150.0 28 24968.0 29 30027.0 30 36486.0 31 44489.0 32 55501.0 33 70123.0 34 92324.0 35 124556.0 36 171668.0 37 221901.0 38 175268.0 39 25349.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.72054368810133 18.705444728705288 14.253292106791394 22.32071947640199 2 15.533749838221972 20.34418520740924 40.15342439965644 23.968640554712348 3 19.82674925067404 24.758498242554186 29.564536139391095 25.850216367380675 4 12.693812661420683 16.401007994465914 37.40065740815541 33.504521935957996 5 13.635604260802795 37.803212778957324 33.6156665524157 14.945516407824178 6 31.569897871138792 35.935475267872555 17.58577412421756 14.908852736771097 7 28.621686013296753 33.2092386698224 20.335149761123002 17.833925555757844 8 26.702229991510624 33.98159641092352 19.563620899873992 19.752552697691865 9 26.56766686598459 17.160253180847274 20.51193317984284 35.760146773325296 10-11 25.197338170930166 26.63688678046109 26.834578041557926 21.331197007050818 12-13 26.55284862804902 24.120222411539796 27.371987065739745 21.954941894671443 14-15 23.03592051417238 25.44072926866009 26.322227034219576 25.201123182947953 16-17 21.947014868572925 28.951306188712937 26.771787924512314 22.32989101820182 18-19 21.818157565600025 27.312772565137085 29.27488846308964 21.594181406173252 20-21 22.93176385333534 26.159032587963658 29.232035524268436 21.677168034432565 22-23 22.349541325773394 26.38160048778862 29.2452804294422 22.02357775699579 24-25 22.393505946020138 26.52511249889707 29.391633415358676 21.68974813972412 26-27 22.154177982137078 26.57124089451857 29.47668114393278 21.797899979411564 28-29 21.703239830245316 27.049751735343293 29.481153528871157 21.765854905540237 30-31 21.927551658502065 26.642045443407454 29.346812276894667 22.083590621195814 32-33 21.7256218178824 26.853435853802466 29.307026194084422 22.113916134230717 34-35 22.502499676512453 26.738186822881744 28.95472350636976 21.80458999423604 36-37 22.500685146609612 26.414509189982233 29.11279101087648 21.972014652531673 38-39 22.149886942362365 26.740611236600014 29.211646955912812 21.897854865124806 40-41 22.276289267711075 26.79423980126538 29.141549407046437 21.787921523977108 42-43 22.040631816991375 26.98764061331163 28.582042270639363 22.38968529905764 44-45 22.196377009042774 26.990792561126455 28.507579425676933 22.305251004153835 46-47 22.487204267139223 27.26374205841245 28.047854374660623 22.201199299787703 48-49 22.64806641141107 27.331977859726514 27.812326759573846 22.207628969288564 50-51 22.359908744507752 27.65002993365197 27.83857077495422 22.15149054688606 52-53 22.6591533436914 27.73817378891577 27.493806526836686 22.108866340556144 54-55 22.144551662923188 27.450670591404027 27.718522938581753 22.68625480709103 56-57 22.492271320276096 27.819522341128337 27.41561183502479 22.27259450357078 58-59 22.807867713018553 27.320495448871156 27.42243320724417 22.449203630866126 60-61 22.431478517663496 27.40153553362185 28.14107274063189 22.025913208082763 62-63 22.42606065073214 27.516345148051208 27.942714745213294 22.11487945600336 64-65 22.119024329044894 27.805563668939936 27.769729736087395 22.30568226592777 66-67 22.09804521182753 27.79786408223754 27.42631318952742 22.67777751640752 68-69 22.079410901012974 27.71457931386529 27.77789757496941 22.428112210152328 70-71 22.284015428393545 27.643139594969384 27.501448666246898 22.57139631039017 72-73 22.21334273391428 27.530971136739634 27.663274641135565 22.592411488210516 74-75 22.100860402844038 28.166459306823427 27.318172603641038 22.414507686691497 76-77 22.32216699508838 27.72698842190916 27.360567826436476 22.590276756565984 78-79 22.286503905131845 27.83897477643228 27.28090834017584 22.593612978260033 80-81 22.357542990298388 28.041629531983403 27.264692885550158 22.336134592168055 82-83 22.3199397463056 28.050117837375403 27.296358548688932 22.333583867630065 84-85 22.241063289650373 28.010076159297416 27.082206953334055 22.666653597718163 86-87 22.37939048736233 27.90214802001703 27.130494921250765 22.587966571369876 88-89 22.328644939965695 27.8298064199951 26.984052928203873 22.857495711835334 90-91 22.242388455765767 27.733537531151146 27.30687073901731 22.717203274065778 92-93 22.36710751773272 27.790320620145316 27.354423083709424 22.488148778412544 94-95 22.078174664482294 27.720314731409385 27.334350754713878 22.86715984939445 96-97 21.950081440951976 27.537942386702184 27.557587026538695 22.954389145807145 98-99 21.99022522453631 27.80215001139201 27.02286597859714 23.18475878547454 100-101 21.837450620396826 27.72959309716871 27.256129266527118 23.17682701590735 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 370.0 1 270.5 2 238.0 3 334.0 4 311.0 5 317.0 6 464.0 7 816.0 8 1281.0 9 1536.5 10 1463.5 11 1330.5 12 1376.5 13 1521.0 14 1670.0 15 1695.5 16 1773.0 17 1922.0 18 2018.5 19 2183.5 20 2417.0 21 2814.0 22 3348.0 23 3960.0 24 4694.5 25 5741.5 26 7166.5 27 8780.0 28 10558.0 29 12596.5 30 15126.0 31 17996.5 32 21137.5 33 23122.5 34 24917.5 35 27113.0 36 29590.5 37 32545.0 38 33950.0 39 34557.0 40 35877.0 41 38220.0 42 40366.5 43 42276.0 44 44228.5 45 46234.0 46 46958.5 47 49184.0 48 57680.0 49 57200.5 50 48820.0 51 44984.0 52 41914.5 53 40725.0 54 39974.0 55 37348.5 56 34016.0 57 30719.5 58 28009.0 59 24999.0 60 21170.5 61 17594.0 62 14718.5 63 11727.0 64 8884.0 65 6792.0 66 4990.5 67 3925.5 68 2906.5 69 1887.0 70 1382.5 71 1079.0 72 883.5 73 771.5 74 623.5 75 500.0 76 365.0 77 208.0 78 111.5 79 69.0 80 51.5 81 38.0 82 30.5 83 18.0 84 9.0 85 9.5 86 6.0 87 2.5 88 1.5 89 1.0 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08429157829119407 2 0.034500739021511996 3 0.014819635625149472 4 0.025718732725127126 5 0.028541520463250834 6 0.037245115989132264 7 0.08146879055307037 8 0.06280702717325251 9 0.07754825202789856 10-11 0.06845260264949993 12-13 0.045556657662496516 14-15 0.05825920248405321 16-17 0.0707265149940996 18-19 0.06813895956748618 20-21 0.02062203264240376 22-23 0.015094073321911497 24-25 0.02528747348735822 26-27 0.02724774274994413 28-29 0.006508093951785217 30-31 9.801346312929545E-4 32-33 0.01117353479673968 34-35 0.01332983098558418 36-37 0.004743851615457899 38-39 0.007880282435595354 40-41 0.005214316238478517 42-43 0.0022739123445996543 44-45 5.880807787757727E-4 46-47 5.880807787757727E-4 48-49 9.409292460412363E-4 50-51 0.0011369561722998271 52-53 3.9205385251718176E-5 54-55 4.312592377688999E-4 56-57 0.004116565451430409 58-59 0.003685306213661509 60-61 0.0061160400992680344 62-63 0.0025875554266133996 64-65 5.880807787757727E-4 66-67 3.920538525171818E-4 68-69 0.002469939270858245 70-71 0.0018818584920824726 72-73 0.018544147224062696 74-75 0.013839500993856517 76-77 0.023327204224772313 78-79 0.017093547969749125 80-81 0.01054624863271219 82-83 0.004743851615457899 84-85 0.0037245115989132265 86-87 0.0016466261805721636 88-89 0.0017642423363273178 90-91 0.007919487820847072 92-93 0.011761615575515453 94-95 0.016936726428742253 96-97 0.013957117149611672 98-99 0.02497383040534448 100-101 0.020700443412907197 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1275335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.82503242050327 #Duplication Level Percentage of deduplicated Percentage of total 1 82.9217117321053 52.92480939665246 2 10.456774708728878 13.348079695970384 3 2.968030487981088 5.683039263613052 4 1.2197543832517719 3.1140345222438124 5 0.6246168986385694 1.9933096903000442 6 0.3686964134704909 1.4119236325846416 7 0.2543984983052135 1.1365894681440316 8 0.1776745030012072 0.9072064731479084 9 0.13940185757813828 0.800759527146275 >10 0.7657566292218292 9.163215170008682 >50 0.06780475179825018 2.9599526012815933 >100 0.03191546149122764 3.8290874617928043 >500 0.0022266478466331533 0.9336223192109926 >1k 0.001237026581462863 1.7943707779034443 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4982 0.3906424586481199 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4140 0.3246205898842265 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3251 0.2549134149066716 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2349 0.18418689991257198 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1832 0.1436485315622954 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1363 0.10687388019618375 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1317 0.10326698475302569 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.841077050343635E-5 0.0 10-11 0.0 0.0 0.0 7.841077050343635E-5 0.0 12-13 0.0 0.0 0.0 7.841077050343635E-5 0.0 14-15 3.9205385251718176E-5 0.0 0.0 7.841077050343635E-5 0.0 16-17 7.841077050343635E-5 0.0 0.0 7.841077050343635E-5 0.0 18-19 7.841077050343635E-5 0.0 0.0 1.568215410068727E-4 0.0 20-21 7.841077050343635E-5 0.0 0.0 3.136430820137454E-4 0.0 22-23 7.841077050343635E-5 0.0 0.0 5.488753935240545E-4 0.0 24-25 7.841077050343635E-5 0.0 0.0 6.272861640274908E-4 0.0 26-27 7.841077050343635E-5 0.0 0.0 7.449023197826453E-4 0.0 28-29 7.841077050343635E-5 0.0 0.0 0.0010585454017963908 0.0 30-31 7.841077050343635E-5 0.0 0.0 0.001999474647837627 0.0 32-33 7.841077050343635E-5 0.0 0.0 0.0030580200496340177 0.0 34-35 7.841077050343635E-5 0.0 0.0 0.004273386992437281 0.0 36-37 7.841077050343635E-5 0.0 0.0 0.005880807787757726 0.0 38-39 7.841077050343635E-5 0.0 0.0 0.009174060148902053 0.0 40-41 7.841077050343635E-5 0.0 0.0 0.011879231731270607 0.0 42-43 7.841077050343635E-5 0.0 0.0 0.018622557994566132 0.0 44-45 7.841077050343635E-5 0.0 0.0 0.026110786577644304 0.0 46-47 7.841077050343635E-5 0.0 0.0 0.03457914979201543 0.0 48-49 7.841077050343635E-5 0.0 0.0 0.04453731764595185 0.0 50-51 7.841077050343635E-5 0.0 0.0 0.057161451697005095 0.0 52-53 7.841077050343635E-5 0.0 0.0 0.08256654134011848 0.0 54-55 7.841077050343635E-5 0.0 0.0 0.10385506553180146 0.0 56-57 7.841077050343635E-5 0.0 0.0 0.14705940007919488 3.9205385251718176E-5 58-59 7.841077050343635E-5 0.0 0.0 0.19508599701254964 7.841077050343635E-5 60-61 7.841077050343635E-5 0.0 0.0 0.24554332783151095 7.841077050343635E-5 62-63 7.841077050343635E-5 0.0 0.0 0.3112515535133906 7.841077050343635E-5 64-65 7.841077050343635E-5 0.0 0.0 0.3473597133302231 7.841077050343635E-5 66-67 7.841077050343635E-5 0.0 0.0 0.4381201801879506 7.841077050343635E-5 68-69 7.841077050343635E-5 0.0 0.0 0.5516197704916748 7.841077050343635E-5 70-71 7.841077050343635E-5 0.0 0.0 0.6269333155602254 7.841077050343635E-5 72-73 7.841077050343635E-5 0.0 0.0 0.7567423461286642 7.841077050343635E-5 74-75 7.841077050343635E-5 0.0 0.0 0.8838069997294828 7.841077050343635E-5 76-77 7.841077050343635E-5 0.0 0.0 0.9642172448807568 7.841077050343635E-5 78-79 7.841077050343635E-5 0.0 0.0 1.0507435301312988 7.841077050343635E-5 80-81 7.841077050343635E-5 0.0 0.0 1.2192874813284353 1.568215410068727E-4 82-83 7.841077050343635E-5 0.0 0.0 1.3597995820705933 1.568215410068727E-4 84-85 7.841077050343635E-5 0.0 0.0 1.5863282980550208 1.9602692625859087E-4 86-87 7.841077050343635E-5 0.0 0.0 1.9225928873590077 2.3523231151030904E-4 88-89 7.841077050343635E-5 0.0 0.0 2.3446780649790053 2.3523231151030904E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6375 0.0 24.157425 1 GTATAGC 440 0.0 23.765985 1 TACATGG 6425 0.0 23.449732 2 GAGTACT 4830 0.0 23.181896 12-13 ACATGGG 6435 0.0 22.160297 3 GTACTTT 5325 0.0 20.978197 14-15 AGTACTT 5040 0.0 20.942455 12-13 GTACACG 205 6.786413E-7 20.867693 1 GTATCAA 11400 0.0 20.722271 1 TACCTGG 965 0.0 20.685865 2 TCGCGCT 200 0.0 20.1797 60-61 GTACCTG 1075 0.0 19.897102 1 CTCGCGC 195 0.0 19.47965 60-61 GGTATCA 8960 0.0 19.256813 1 TATCAAC 12925 0.0 18.165533 2 TCAACGC 12965 0.0 18.148283 4 TAAGGTG 420 1.8189894E-12 18.108839 5 CATGGGA 3965 0.0 18.102419 4 CATGGGG 2685 0.0 18.057547 4 ATCAACG 12995 0.0 18.033228 3 >>END_MODULE