FastQCFastQC Report
Thu 26 May 2016
SRR936444_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936444_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1275335
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73210.5740452508556575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64300.5041812543370957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.3177988528504275No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35110.27530021523756504No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19160.15023503628458407No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.14035527920115107No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14610.11455813570552052No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC13550.10624659403215626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC652.588653E-643.848493
TACCTGG10500.023.0753732
ACGTATA1050.002194172222.6229132
GAGTACT72150.022.28286212-13
GGTATCA98800.021.9311241
TAACGCA1302.9438146E-421.9208054
GTATCAA127150.021.8621331
TACAACG1353.7918816E-421.114722
ATACGTC2257.1609975E-821.1089255
GTACTTT77800.020.66382214-15
GTACATG60450.020.594821
TACATGG60500.020.259662
AGTACTT75350.020.23347312-13
TGCACCG2902.4429028E-919.6531375
GTCTAGC3404.1836756E-1119.5660041
ACATGGG58700.019.259973
GTGTAGC6250.019.0069751
ACCGTGC2001.2860624E-518.9980348
TACCGTA1250.00603510718.9980347
TCAACGC147450.018.8112094
Produced by FastQC (version 0.11.5)