##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936444_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1275335 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87436791117628 31.0 31.0 34.0 30.0 34.0 2 32.02680629011201 31.0 31.0 34.0 30.0 34.0 3 32.102868658038865 33.0 31.0 34.0 30.0 34.0 4 35.81251749540317 37.0 35.0 37.0 35.0 37.0 5 35.64336664484234 37.0 35.0 37.0 33.0 37.0 6 35.73682522631309 37.0 35.0 37.0 33.0 37.0 7 35.68334123975269 37.0 35.0 37.0 33.0 37.0 8 35.65473620656533 37.0 35.0 37.0 33.0 37.0 9 37.28153073506177 39.0 37.0 39.0 34.0 39.0 10-11 37.17788894682573 39.0 37.0 39.0 33.0 39.0 12-13 37.065867007492145 39.0 37.0 39.0 33.0 39.0 14-15 38.18150250718439 40.0 37.5 41.0 33.0 41.0 16-17 38.087072808320954 40.0 37.0 41.0 33.0 41.0 18-19 38.252757118717824 40.0 37.5 41.0 33.5 41.0 20-21 38.28502903158778 40.0 38.0 41.0 34.0 41.0 22-23 38.202144534573264 40.0 37.5 41.0 33.0 41.0 24-25 38.05948280255776 40.0 37.0 41.0 33.0 41.0 26-27 37.859364402294304 40.0 37.0 41.0 33.0 41.0 28-29 37.76224286167948 40.0 37.0 41.0 33.0 41.0 30-31 37.701662308334676 40.0 37.0 41.0 33.0 41.0 32-33 37.52235961531676 40.0 36.0 41.0 32.5 41.0 34-35 37.32890534643839 39.0 36.0 41.0 32.0 41.0 36-37 37.10887609922099 39.0 36.0 41.0 31.0 41.0 38-39 36.928426648684464 39.0 35.0 41.0 31.0 41.0 40-41 36.72268109947582 39.0 35.0 41.0 31.0 41.0 42-43 36.42444847824297 39.0 35.0 40.5 30.0 41.0 44-45 36.10776854708763 39.0 35.0 40.0 30.0 41.0 46-47 35.65807297690411 38.5 35.0 40.0 28.5 41.0 48-49 35.21614673791592 38.0 34.5 40.0 26.5 41.0 50-51 34.90926540869654 38.0 34.0 40.0 26.0 41.0 52-53 34.54515127397899 38.0 34.0 40.0 24.0 41.0 54-55 34.360994170159216 38.0 34.0 40.0 24.0 41.0 56-57 34.059541610635634 38.0 33.0 40.0 23.5 41.0 58-59 33.68258928046357 37.0 33.0 40.0 22.5 41.0 60-61 33.27390136709178 37.0 32.5 39.5 22.0 41.0 62-63 32.95796751441778 36.0 32.0 39.0 21.0 40.5 64-65 32.53945473150192 36.0 31.5 39.0 19.5 40.0 66-67 32.13666526834126 35.0 31.0 38.5 18.5 40.0 68-69 31.790854559782332 35.0 31.0 38.0 18.0 40.0 70-71 31.421850729416192 35.0 31.0 37.5 16.0 40.0 72-73 30.976960955356827 34.5 30.0 37.0 12.5 39.0 74-75 30.488646512484955 34.0 29.5 36.5 8.0 39.0 76-77 28.98321931100456 32.5 28.0 35.0 7.5 37.0 78-79 29.741036668796827 34.0 29.0 35.0 7.0 37.5 80-81 29.51403788024323 34.0 29.0 35.0 4.0 37.0 82-83 29.209141127625294 34.0 29.0 35.0 2.0 36.5 84-85 28.839789545491968 34.0 29.0 35.0 2.0 36.0 86-87 28.46094359521224 33.5 29.0 35.0 2.0 36.0 88-89 27.996986674089552 33.0 27.0 35.0 2.0 35.0 90-91 27.518631575233172 33.0 26.5 35.0 2.0 35.0 92-93 26.912041934080065 32.5 25.5 34.5 2.0 35.0 94-95 26.38670702207655 32.0 25.0 34.0 2.0 35.0 96-97 25.686275762838783 32.0 23.5 34.0 2.0 35.0 98-99 24.823450309134465 32.0 18.5 34.0 2.0 35.0 100-101 21.64570563812645 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3082784563462688 1101 2 0.3439612162396912 1101 3 0.37721760973020224 1101 4 0.21537634534749373 1101 5 0.27989376870471716 1101 6 0.3016756805921119 1101 7 0.3605297347148806 1101 8 0.234347921807732 1101 9 0.3219817160879188 1101 10-11 0.3045978070124704 1101 12-13 0.358343834054466 1101 14-15 0.5741097832738475 1101 16-17 0.49770773472795327 1101 18-19 0.4455504526663603 1101 20-21 0.3801970580381351 1101 22-23 0.3496058580925876 1101 24-25 0.2686240834452889 1101 26-27 0.3897638501961751 1101 28-29 0.2532351634231489 1101 30-31 0.34817743534026846 1101 32-33 0.2675253857725153 1101 34-35 0.25381330236950106 1101 36-37 0.1042920904539244 1101 38-39 0.07964905583555293 1101 40-41 0.13629467166460074 1101 42-43 0.2377774224500584 1101 44-45 0.22353365203186826 1101 46-47 0.31646915250779983 1101 48-49 0.2083080649063831 1101 50-51 0.21237501890798427 1101 52-53 0.3351120804665513 1101 54-55 0.23492041117653883 1101 56-57 0.4349394530059385 1101 58-59 0.15974424618237748 1101 60-61 0.4346947508566643 1101 62-63 0.23241818725689 1101 64-65 0.3390336587030802 1101 66-67 0.22551051469630323 1101 68-69 0.2338713574322746 1101 70-71 0.06740236287553714 1101 72-73 0.300929222434835 1101 74-75 0.16674356590410966 1101 76-77 0.027035834558787997 1101 78-79 0.11597435566017111 1101 80-81 0.21020250097635795 1101 82-83 0.4265486301922188 1101 84-85 0.3013794892029473 1101 86-87 0.2708194796999557 1101 88-89 0.3391667573751711 1101 90-91 0.3942728524081467 1101 92-93 0.48786696482558867 1101 94-95 0.3273289949376803 1101 96-97 0.5512307566709538 1101 98-99 0.22201932461285878 1101 100-101 0.8926618315375059 1102 1 0.26299754487129334 1102 2 0.2258917430528058 1102 3 0.25806659824422695 1102 4 0.21205770467838647 1102 5 0.23680532641379415 1102 6 0.14682846216491896 1102 7 0.16530145774092375 1102 8 0.17157080143203274 1102 9 0.23087939614200081 1102 10-11 0.16318980417336348 1102 12-13 0.26504251323874684 1102 14-15 0.2721368875924881 1102 16-17 0.2539141969601104 1102 18-19 0.20427841775232736 1102 20-21 0.21375528094062446 1102 22-23 0.29618755478504966 1102 24-25 0.1959702377520074 1102 26-27 0.2747645414662543 1102 28-29 0.19298988773702064 1102 30-31 0.1954018499107164 1102 32-33 0.17023647080526416 1102 34-35 -0.01858575314873434 1102 36-37 0.18433953226476518 1102 38-39 0.13757150077616842 1102 40-41 -0.018417558590385852 1102 42-43 0.13779282634229872 1102 44-45 0.19937975027495725 1102 46-47 0.1496545250152792 1102 48-49 0.18823646674439942 1102 50-51 0.1784132466874624 1102 52-53 0.05706747123009848 1102 54-55 -0.09381225672316162 1102 56-57 -0.23132624072985664 1102 58-59 -0.07611084401475665 1102 60-61 0.08629572146517006 1102 62-63 0.16660873177087154 1102 64-65 0.06630067075077761 1102 66-67 -0.20884822072579112 1102 68-69 0.00582380893723311 1102 70-71 0.00900745933220648 1102 72-73 0.20526805798767356 1102 74-75 -0.01770470804349955 1102 76-77 -0.0931806581654584 1102 78-79 0.18789365885993803 1102 80-81 -0.029967856274190297 1102 82-83 0.10324896407022521 1102 84-85 -0.1032804875660247 1102 86-87 -0.04317144561130348 1102 88-89 0.04141659653240737 1102 90-91 -0.1409370160981389 1102 92-93 0.007632662371523935 1102 94-95 -0.03087246184596637 1102 96-97 0.27893702458546343 1102 98-99 0.15306513459087867 1102 100-101 0.8248202053219309 1103 1 0.21878548074457527 1103 2 0.2686813320728376 1103 3 0.17515936668834797 1103 4 0.2180167220172109 1103 5 0.29964412538345897 1103 6 0.2616068251651882 1103 7 0.2438835960670147 1103 8 0.09583361331930718 1103 9 0.2760141468244015 1103 10-11 0.17869286057258194 1103 12-13 0.2610442048307604 1103 14-15 0.34554813667941886 1103 16-17 0.28764992415244706 1103 18-19 0.2577365700725025 1103 20-21 0.16610236486665286 1103 22-23 0.1438001033222065 1103 24-25 0.30756725439881905 1103 26-27 0.28868173034787503 1103 28-29 0.21033831724761143 1103 30-31 0.19532495936440597 1103 32-33 0.23878439283824804 1103 34-35 0.33295782456838197 1103 36-37 0.24972533058086555 1103 38-39 0.36509062729955843 1103 40-41 0.5538499436202144 1103 42-43 0.49626616454536787 1103 44-45 0.3197807344739445 1103 46-47 0.222806268508414 1103 48-49 0.18291179641261124 1103 50-51 0.3336648710002592 1103 52-53 0.34632970643827576 1103 54-55 0.14245316944268183 1103 56-57 0.07854266088869366 1103 58-59 0.1350305961451781 1103 60-61 0.14713536623084167 1103 62-63 0.19667293537703756 1103 64-65 0.12240193560026569 1103 66-67 0.08195182540853807 1103 68-69 0.07182473162378855 1103 70-71 0.19965756890123743 1103 72-73 0.2656848047486591 1103 74-75 0.2788525177455945 1103 76-77 0.15217707510123546 1103 78-79 -0.03835677364938661 1103 80-81 0.09228811235446699 1103 82-83 0.35123058722965794 1103 84-85 0.4178067447587601 1103 86-87 0.5962599487987497 1103 88-89 0.3754483523280321 1103 90-91 0.432022501029234 1103 92-93 0.5676649564009715 1103 94-95 0.5484401366700453 1103 96-97 0.6187644053090047 1103 98-99 0.28651828902554044 1103 100-101 0.26245236094839797 1104 1 0.12821577850191446 1104 2 0.13858741791796092 1104 3 0.10247165868264574 1104 4 0.11203655197464713 1104 5 0.05400346299897052 1104 6 0.05542011712675787 1104 7 0.028063902005030172 1104 8 0.0363803134272942 1104 9 0.062025961644707195 1104 10-11 0.13888569353088798 1104 12-13 0.08325454635953378 1104 14-15 0.04856170505157564 1104 16-17 0.20349242786262778 1104 18-19 0.08377546774091371 1104 20-21 0.08691294469368671 1104 22-23 0.19689364982789925 1104 24-25 0.035100713361657654 1104 26-27 0.00312947700487598 1104 28-29 0.018854207969184245 1104 30-31 0.14532900158054218 1104 32-33 -0.11423774118238583 1104 34-35 -0.10049045527616585 1104 36-37 -0.08433736611625875 1104 38-39 -0.008677699819671147 1104 40-41 0.06668253620140518 1104 42-43 -0.0017863534665636394 1104 44-45 0.05290592575739339 1104 46-47 0.09499331917277942 1104 48-49 0.21080116288185025 1104 50-51 0.30566578723591675 1104 52-53 0.2846320141587171 1104 54-55 0.2881890163928347 1104 56-57 0.11147902293267009 1104 58-59 0.32802861160953256 1104 60-61 0.5429284779095767 1104 62-63 0.47079919111072854 1104 64-65 0.38758478813444697 1104 66-67 0.27588773125955157 1104 68-69 0.19528556158700283 1104 70-71 0.27935804068560444 1104 72-73 0.37189403370555496 1104 74-75 0.21360826202344896 1104 76-77 0.06937564608158908 1104 78-79 0.15582359003457213 1104 80-81 0.3365650080094653 1104 82-83 0.29484600308708053 1104 84-85 0.3875892542820303 1104 86-87 0.5576664090300802 1104 88-89 0.3706453333700388 1104 90-91 0.4328631857421499 1104 92-93 0.3839115854814459 1104 94-95 0.44835390272582387 1104 96-97 0.643516723327469 1104 98-99 0.605278310353043 1104 100-101 1.0036846016642933 1105 1 -0.04433328125205449 1105 2 -0.0693648876066284 1105 3 -0.04259965781070463 1105 4 0.14711406666511806 1105 5 0.11506966458370016 1105 6 0.035451871253769696 1105 7 -0.030981836222579773 1105 8 0.1667313770788681 1105 9 -0.08747715885904483 1105 10-11 -0.04188714533646731 1105 12-13 0.08519683425740965 1105 14-15 0.19266141226587052 1105 16-17 0.08550192804224821 1105 18-19 -0.025213686344777386 1105 20-21 0.03517986921527694 1105 22-23 0.035465514480122806 1105 24-25 -0.010041891705228068 1105 26-27 -0.0894916903036389 1105 28-29 -0.1119970855554655 1105 30-31 0.09897281557506687 1105 32-33 -0.018903233976281797 1105 34-35 -0.12205664476230993 1105 36-37 -0.21966699666276668 1105 38-39 -0.16445067828605175 1105 40-41 0.04260123744644062 1105 42-43 0.2768156273506719 1105 44-45 0.3207248118315462 1105 46-47 0.17585187184722173 1105 48-49 0.18656630518911754 1105 50-51 0.1184504529334518 1105 52-53 0.2909930768237885 1105 54-55 -0.013714835237585987 1105 56-57 0.019592611874941213 1105 58-59 0.23716313482299256 1105 60-61 0.31654491188016465 1105 62-63 0.19582864650697474 1105 64-65 0.07835803649229689 1105 66-67 0.20145538746621838 1105 68-69 0.19709988370339815 1105 70-71 0.07439268916592923 1105 72-73 0.2362154055731871 1105 74-75 0.10121335682980614 1105 76-77 -0.06888230687540187 1105 78-79 0.03087687033268338 1105 80-81 0.045310236049143526 1105 82-83 0.03551008500006958 1105 84-85 0.21289548803037306 1105 86-87 0.3650397508262202 1105 88-89 0.1211618497971223 1105 90-91 0.2642061470657744 1105 92-93 0.1670058632239595 1105 94-95 0.38031659069592294 1105 96-97 0.5026864254975827 1105 98-99 0.23040976611038744 1105 100-101 0.485419464010338 1106 1 0.13167636990811715 1106 2 0.1694083101294055 1106 3 0.07648487252205172 1106 4 0.08255220227744786 1106 5 0.1747646082476848 1106 6 -0.08524682313367293 1106 7 0.10388542274269952 1106 8 0.1310816525578602 1106 9 0.02037962964976714 1106 10-11 0.10436805043025998 1106 12-13 0.174942194710205 1106 14-15 0.17746919708408626 1106 16-17 0.027638186789545216 1106 18-19 0.14230281232855901 1106 20-21 0.0588483248041598 1106 22-23 -0.08233651647273632 1106 24-25 -0.10159449578968349 1106 26-27 -0.16084469426482428 1106 28-29 -0.3276453631540974 1106 30-31 -0.16579424871338944 1106 32-33 -0.1324063082165523 1106 34-35 -0.11600165043724786 1106 36-37 -0.3787866477477735 1106 38-39 -0.08270191286786854 1106 40-41 -0.08675550220524286 1106 42-43 -0.009179855244916268 1106 44-45 -0.1645997309785585 1106 46-47 -0.24494416095469518 1106 48-49 -0.49344305282834 1106 50-51 -0.4618363571476536 1106 52-53 -0.5481467372184952 1106 54-55 -0.4163463548443289 1106 56-57 -0.5881293497759827 1106 58-59 -0.6697701601762915 1106 60-61 -0.6303201522729935 1106 62-63 -0.6717981565086362 1106 64-65 -0.6349169125835488 1106 66-67 -0.5299850143647973 1106 68-69 -0.5734435953744814 1106 70-71 -0.4086905332993247 1106 72-73 -0.33617957041166235 1106 74-75 -0.5102621469622584 1106 76-77 -0.5770376875475236 1106 78-79 -0.4643783517417148 1106 80-81 -0.30855060384232047 1106 82-83 -0.44351470810882176 1106 84-85 0.1149040793668803 1106 86-87 0.0872625994217664 1106 88-89 -0.17567427590848794 1106 90-91 -0.10138040144183336 1106 92-93 0.15558926984297372 1106 94-95 0.08735755926952393 1106 96-97 0.25847740346678094 1106 98-99 0.33723914577530323 1106 100-101 0.7683759398335788 1107 1 0.014186454879361321 1107 2 0.05705743895955351 1107 3 0.03862689180508738 1107 4 0.16907910983903207 1107 5 -0.028443986150506362 1107 6 -0.018868400680666753 1107 7 0.028244562158484143 1107 8 0.1303342202916724 1107 9 0.01034830186674185 1107 10-11 0.05351225836663076 1107 12-13 -0.08229967294064267 1107 14-15 -0.013285173070123335 1107 16-17 0.09684183168412375 1107 18-19 0.07577240006065722 1107 20-21 0.08833077266270806 1107 22-23 -0.07724396361604136 1107 24-25 0.058057393890749154 1107 26-27 0.017569891035435603 1107 28-29 0.14019716824520145 1107 30-31 0.095647902174953 1107 32-33 0.1504377967243684 1107 34-35 0.19319033594445756 1107 36-37 0.19902498501623 1107 38-39 0.16089842186733705 1107 40-41 0.1791855472039643 1107 42-43 0.22265289158654156 1107 44-45 0.003046822965309559 1107 46-47 -0.2919072256415589 1107 48-49 0.016507809758564918 1107 50-51 -0.11027676283529075 1107 52-53 0.07966775653025593 1107 54-55 0.0821301707757911 1107 56-57 -0.045666971725452754 1107 58-59 -0.005606855166703895 1107 60-61 0.11500213765919653 1107 62-63 0.07860238135649666 1107 64-65 0.05798140801839935 1107 66-67 0.15784757511249836 1107 68-69 0.20131465141406935 1107 70-71 0.1115455694672498 1107 72-73 0.12044432453297915 1107 74-75 0.08787499434177803 1107 76-77 -0.05406040376644228 1107 78-79 0.29684743717483286 1107 80-81 0.5853712340542856 1107 82-83 0.35870183982698123 1107 84-85 0.6905789141450107 1107 86-87 0.5694726386172562 1107 88-89 0.5180179679618497 1107 90-91 0.31471764450558126 1107 92-93 0.21970036188085373 1107 94-95 0.44157250505385903 1107 96-97 0.6731950242718625 1107 98-99 0.42579377627912507 1107 100-101 1.1462422889309387 1108 1 0.13123158658113354 1108 2 0.16955416440221427 1108 3 0.10420151666072996 1108 4 0.14099355441876327 1108 5 0.22262647125442214 1108 6 0.05092763590582905 1108 7 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2316 58-59 -1.8439385045344565 2316 60-61 -1.2858601292646554 2316 62-63 -1.1030247886627897 2316 64-65 -0.9621266719546568 2316 66-67 -1.0769603762668218 2316 68-69 -1.523550248619614 2316 70-71 -1.7432162259462416 2316 72-73 -1.1286371487838203 2316 74-75 -1.1185811000827606 2316 76-77 -1.212756937859094 2316 78-79 -1.320174775862128 2316 80-81 -1.2108670164585575 2316 82-83 -1.5558541290655832 2316 84-85 -1.401687091182442 2316 86-87 -1.5501463258969963 2316 88-89 -1.8561878891766277 2316 90-91 -1.9450914550320917 2316 92-93 -1.9883746358228294 2316 94-95 -2.2886370721176874 2316 96-97 -2.6446898384084 2316 98-99 -3.067707647437235 2316 100-101 -4.183612124589775 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 15.0 5 51.0 6 86.0 7 233.0 8 511.0 9 1074.0 10 1788.0 11 2780.0 12 3954.0 13 5521.0 14 7288.0 15 9673.0 16 12295.0 17 16313.0 18 14233.0 19 9172.0 20 8039.0 21 8434.0 22 8843.0 23 9616.0 24 11284.0 25 13747.0 26 17009.0 27 20879.0 28 25673.0 29 31585.0 30 38855.0 31 48181.0 32 59107.0 33 75226.0 34 98532.0 35 133225.0 36 186119.0 37 239452.0 38 147938.0 39 8594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.59462632673245 19.194199984157247 15.013595747868091 22.197577941242212 2 15.973781435104112 20.68123410452449 39.359765733138744 23.98521872723266 3 19.8837797461505 24.60936427834829 30.331447302898056 25.175408672603155 4 12.999329587912195 16.985576338765892 37.07551349253333 32.93958058078857 5 13.506155414412296 37.710029012781305 33.77079902767976 15.013016545126638 6 31.699984709899752 36.18366938882725 17.03152505028091 15.084820850992092 7 28.274845432768647 33.489318492788165 20.266753441252693 17.969082633190496 8 26.957387666769904 33.7273735920366 19.35554187723226 19.959696863961234 9 26.473044337370183 16.729094708449153 20.536643313325516 36.26121764085515 10-11 25.251914202934916 26.568078191220344 26.64554803247774 21.534459573367 12-13 26.69677935860045 23.99432142743726 27.544409424924947 21.764489789037345 14-15 23.131418669248998 25.193429948660444 26.88020575026513 24.794945631825428 16-17 21.809141052798307 28.544628879717436 27.50004018553564 22.14618988194862 18-19 21.546363055401997 26.52744616036038 30.562684513237905 21.363506270999714 20-21 22.43147878792631 25.250698835992115 30.840916308264106 21.476906067817474 22-23 21.86164565517739 25.5327623979287 30.831923245204784 21.773668701689125 24-25 22.078081445267323 25.573163129687494 30.96272744024119 21.38602798480399 26-27 21.980524712255896 25.646735672192257 30.951687376034897 21.42105223951695 28-29 21.372631661488096 26.1958185899258 30.954863119100594 21.47668662948551 30-31 21.651689362304396 25.77309119251387 30.808892053240033 21.7663273919417 32-33 21.52075270330725 25.866863857765203 30.847990991524366 21.764392447403182 34-35 22.223995207451033 25.73285930591157 30.539287218118215 21.50385826851918 36-37 22.17629946758878 25.422400476739355 30.67131644358715 21.729983612084716 38-39 21.909593209826447 25.825532310290768 30.72773870895274 21.53713577093005 40-41 21.858081604665138 25.910596578222993 30.636426685924405 21.59489513118746 42-43 21.8489187280759 25.9870279518898 30.096476814316535 22.067576505717764 44-45 22.023381756834905 26.020173126466062 29.848534925416203 22.10791019128283 46-47 22.358038815021356 26.454230313542134 29.242016386334214 21.9457144851023 48-49 22.67658881613639 26.63298122797381 28.732793158782226 21.95763679710757 50-51 22.294199009828013 26.803566751925377 28.873181456779168 22.029052781467442 52-53 22.646878363093546 27.074821664154253 28.317438785571607 21.960861187180598 54-55 22.110816369577133 26.750564761545558 28.63009745812218 22.50852141075513 56-57 22.436904342725306 27.05782850403124 28.27770793943068 22.22755921381278 58-59 22.84058725767856 26.580672637334107 28.398578522067652 22.180161582919677 60-61 22.44622348818752 26.57776850611564 29.16660131190534 21.809406693791505 62-63 22.318866606178428 26.769927986580704 28.936917875843314 21.97428753139755 64-65 22.145937522791957 26.935561409466242 28.733782911582505 22.184718156159295 66-67 22.131072020726 27.022013923711675 28.4199915863363 22.426922469226028 68-69 22.263004807004325 26.771654160718843 28.694299067728927 22.2710419645479 70-71 22.36561837795242 26.816653696192954 28.496490131005054 22.321237794849576 72-73 22.330524920903137 26.74775647182897 28.54606828794003 22.375650319327864 74-75 22.221577501079146 27.19693848066497 28.134001456111413 22.447482562144476 76-77 22.28849815103489 26.940394006781105 28.24360860278458 22.527499239399422 78-79 22.317151301617525 26.931488738030506 28.255690582259856 22.495669378092114 80-81 22.47237360005458 27.226796451210415 28.013534210780154 22.287295737954842 82-83 22.386272334688893 27.23868409092959 28.06464654069406 22.310397033687458 84-85 22.18113979168489 27.19147946556063 28.023830075607272 22.60355066714721 86-87 22.224439743647554 27.154774064409633 27.827924694017987 22.792861497924832 88-89 22.15625088249414 27.127399207402597 27.67497701593039 23.04137289417287 90-91 22.224924365060716 26.849158062411917 28.024132576192912 22.901784996334456 92-93 22.228980702251828 26.807269196272927 28.148820945902226 22.814929155573022 94-95 22.018465197158452 26.79602710404184 28.17793635617077 23.007571342628943 96-97 21.82254441214439 26.57522930273845 28.440521715458672 23.16170456965849 98-99 21.839603934762682 26.750721871338424 27.928506788395296 23.4811674055036 100-101 21.492714842608354 26.85989140425572 28.092999572003624 23.554394181132306 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 446.0 1 350.0 2 394.5 3 511.0 4 392.0 5 316.0 6 505.5 7 1091.0 8 1847.5 9 2185.5 10 1923.0 11 1607.5 12 1637.0 13 1772.0 14 1945.0 15 2138.0 16 2264.0 17 2397.5 18 2508.0 19 2719.5 20 3022.0 21 3374.0 22 3882.0 23 4452.0 24 5166.0 25 6185.0 26 7538.5 27 9095.0 28 10755.0 29 12691.5 30 15097.5 31 17813.0 32 20914.5 33 22989.0 34 24768.5 35 26894.0 36 29072.5 37 31822.5 38 33535.0 39 34127.0 40 35211.5 41 37652.5 42 39974.5 43 41622.0 44 43426.0 45 45227.5 46 46174.5 47 49447.5 48 58204.5 49 57420.5 50 48730.0 51 44174.5 52 41499.0 53 40775.5 54 39119.0 55 36467.0 56 33292.5 57 30235.0 58 28347.0 59 25323.5 60 21240.0 61 17667.0 62 14902.5 63 11815.0 64 8446.0 65 6420.5 66 5007.5 67 4124.5 68 3018.5 69 1839.5 70 1396.0 71 1091.0 72 891.5 73 792.5 74 646.5 75 555.0 76 431.0 77 260.0 78 163.0 79 122.5 80 84.0 81 56.0 82 41.0 83 28.0 84 20.5 85 14.0 86 6.0 87 3.5 88 2.5 89 1.5 90 1.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02383687423304465 2 0.016309440264714762 3 0.01332983098558418 4 0.0 5 0.0027443769676202723 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0011369561722998271 14-15 1.960269262585909E-4 16-17 3.9205385251718176E-5 18-19 0.0015682154100687272 20-21 0.0 22-23 7.841077050343635E-5 24-25 0.0 26-27 0.002077885418341063 28-29 0.0037245115989132265 30-31 0.0012937777133066998 32-33 0.007174585501064426 34-35 0.0017250369510755997 36-37 3.920538525171818E-4 38-39 0.0016858315658238815 40-41 2.7443769676202724E-4 42-43 0.004273386992437281 44-45 0.0019210638773341907 46-47 3.136430820137454E-4 48-49 5.096700082723363E-4 50-51 7.841077050343635E-5 52-53 1.960269262585909E-4 54-55 0.0014113938690618543 56-57 0.013173009444577308 58-59 0.025405089643113377 60-61 0.019053817232335034 62-63 0.012232080198536069 64-65 0.01677990488773538 66-67 0.002234706959347936 68-69 6.66491549279209E-4 70-71 1.960269262585909E-4 72-73 0.0 74-75 0.0016074207953204454 76-77 0.0021170908035927816 78-79 0.008938827837391744 80-81 0.015368511018673526 82-83 0.01697593181399397 84-85 0.030697816652095335 86-87 0.010781480944222498 88-89 0.04226340530135219 90-91 0.049124347720402875 92-93 0.030854638193102205 94-95 0.031050665119360794 96-97 0.05139826006500253 98-99 0.054613101655643426 100-101 0.06206212485346987 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1275335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.47809141172444 #Duplication Level Percentage of deduplicated Percentage of total 1 81.41893976781844 49.2406208192381 2 11.276293155823128 13.639373765265475 3 3.1781878922802465 5.766322136188818 4 1.322639195385031 3.199627766529022 5 0.7234208635463446 2.1875556557352227 6 0.4270135371107636 1.5494978238857129 7 0.2883101495625687 1.220551330612108 8 0.20746307598067082 1.0037576698973232 9 0.16102627383595913 0.8764705537866455 >10 0.8769582279430199 9.840012143994656 >50 0.07536071595660229 3.1406612111000314 >100 0.03957096360475098 4.5030203812512095 >500 0.0033843435125177216 1.370953250573295 >1k 0.0011715035235638268 1.3790616734736136 >5k 2.6033411634751705E-4 1.0825138184687557 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7321 0.5740452508556575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6430 0.5041812543370957 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.3177988528504275 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3511 0.27530021523756504 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1916 0.15023503628458407 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1790 0.14035527920115107 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1461 0.11455813570552052 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1355 0.10624659403215626 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.841077050343635E-5 0.0 12-13 0.0 0.0 0.0 7.841077050343635E-5 0.0 14-15 0.0 0.0 0.0 7.841077050343635E-5 0.0 16-17 0.0 0.0 0.0 7.841077050343635E-5 0.0 18-19 0.0 0.0 0.0 7.841077050343635E-5 0.0 20-21 0.0 0.0 0.0 2.3523231151030904E-4 0.0 22-23 0.0 0.0 0.0 4.7046462302061814E-4 0.0 24-25 0.0 0.0 0.0 5.488753935240545E-4 0.0 26-27 0.0 0.0 0.0 5.880807787757727E-4 0.0 28-29 0.0 0.0 0.0 9.409292460412363E-4 0.0 30-31 0.0 0.0 0.0 0.0018818584920824726 0.0 32-33 0.0 0.0 0.0 0.002901198508627145 0.0 34-35 0.0 0.0 0.0 0.004038154680926972 0.0 36-37 0.0 0.0 0.0 0.005567164705743981 0.0 38-39 0.0 0.0 0.0 0.008782006296384872 0.0 40-41 7.841077050343635E-5 0.0 0.0 0.011487177878753425 0.0 42-43 7.841077050343635E-5 0.0 0.0 0.018348120297804107 0.0 44-45 7.841077050343635E-5 0.0 0.0 0.026071581192392584 0.0 46-47 7.841077050343635E-5 0.0 0.0 0.034226301324749964 0.0 48-49 7.841077050343635E-5 0.0 0.0 0.044498112260700134 0.0 50-51 7.841077050343635E-5 0.0 0.0 0.05708304092650166 0.0 52-53 7.841077050343635E-5 0.0 0.0 0.08288018442213223 0.0 54-55 7.841077050343635E-5 0.0 0.0 0.10420791399906691 0.0 56-57 7.841077050343635E-5 0.0 0.0 0.14760827547271893 0.0 58-59 7.841077050343635E-5 0.0 0.0 0.19508599701254964 0.0 60-61 7.841077050343635E-5 0.0 0.0 0.24515127397899378 0.0 62-63 7.841077050343635E-5 0.0 0.0 0.31132996428389403 0.0 64-65 7.841077050343635E-5 0.0 0.0 0.3475165348712299 0.0 66-67 7.841077050343635E-5 0.0 0.0 0.4378849478764403 0.0 68-69 7.841077050343635E-5 0.0 0.0 0.5511885112539059 0.0 70-71 7.841077050343635E-5 0.0 0.0 0.6263060293961978 0.0 72-73 7.841077050343635E-5 0.0 0.0 0.7560758545793851 0.0 74-75 7.841077050343635E-5 0.0 0.0 0.8822779897046658 0.0 76-77 7.841077050343635E-5 0.0 0.0 0.9619825379214089 0.0 78-79 7.841077050343635E-5 0.0 0.0 1.0484304124014474 0.0 80-81 7.841077050343635E-5 0.0 0.0 1.2166999259018219 0.0 82-83 7.841077050343635E-5 0.0 0.0 1.3565063297094488 0.0 84-85 7.841077050343635E-5 0.0 0.0 1.5807219279640252 0.0 86-87 7.841077050343635E-5 0.0 0.0 1.9135364433658606 0.0 88-89 7.841077050343635E-5 0.0 0.0 2.332759627862483 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 65 2.588653E-6 43.84849 3 TACCTGG 1050 0.0 23.075373 2 ACGTATA 105 0.0021941722 22.622913 2 GAGTACT 7215 0.0 22.282862 12-13 GGTATCA 9880 0.0 21.931124 1 TAACGCA 130 2.9438146E-4 21.920805 4 GTATCAA 12715 0.0 21.862133 1 TACAACG 135 3.7918816E-4 21.11472 2 ATACGTC 225 7.1609975E-8 21.108925 5 GTACTTT 7780 0.0 20.663822 14-15 GTACATG 6045 0.0 20.59482 1 TACATGG 6050 0.0 20.25966 2 AGTACTT 7535 0.0 20.233473 12-13 TGCACCG 290 2.4429028E-9 19.653137 5 GTCTAGC 340 4.1836756E-11 19.566004 1 ACATGGG 5870 0.0 19.25997 3 GTGTAGC 625 0.0 19.006975 1 ACCGTGC 200 1.2860624E-5 18.998034 8 TACCGTA 125 0.006035107 18.998034 7 TCAACGC 14745 0.0 18.811209 4 >>END_MODULE