FastQCFastQC Report
Thu 26 May 2016
SRR936443_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936443_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884986
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61740.3275355891237388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50770.26933887042131877No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30630.16249457555652932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28570.1515661124273602No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19290.10233497755420995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18800.027.5431182
GTACACG3350.026.9420221
GTACCTG19700.026.5245381
GTACATG85150.025.3833031
GTATCAA124300.024.6496071
TACATGG86700.024.3281062
ACATGGG86000.022.9248053
GAGTACT61950.022.54770512-13
GGTATCA92450.021.4264661
ATCAACG141650.021.3638153
TCAACGC142200.021.3485684
TATCAAC142450.021.343322
CAACGCA142350.021.225955
AACGCAG144450.020.9508156
GTATAGG4800.020.782581
AGTACTT66450.019.76953712-13
GTGTAGC10200.019.5600741
GTACTTT70150.019.54032514-15
ACGCAGA157950.019.1606567
CATGGGA47100.019.0637534
Produced by FastQC (version 0.11.5)