##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936443_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884986 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.37742296229256 31.0 31.0 34.0 30.0 34.0 2 31.452272324568987 31.0 31.0 34.0 28.0 34.0 3 31.460896791806412 31.0 31.0 34.0 28.0 34.0 4 35.09593015544943 37.0 35.0 37.0 32.0 37.0 5 35.104413507580425 37.0 35.0 37.0 32.0 37.0 6 35.093573108765796 37.0 35.0 37.0 32.0 37.0 7 35.00039363687582 37.0 35.0 37.0 32.0 37.0 8 34.956920634954315 37.0 35.0 37.0 32.0 37.0 9 36.45688774346335 39.0 37.0 39.0 32.0 39.0 10-11 36.32022545525537 39.0 36.0 39.0 32.0 39.0 12-13 36.22479901707493 39.0 35.0 39.0 32.0 39.0 14-15 37.31802703043948 40.0 37.0 41.0 32.0 41.0 16-17 37.20982277852461 39.0 37.0 41.0 31.0 41.0 18-19 37.17222064248753 39.0 36.0 41.0 31.0 41.0 20-21 37.03373526381628 39.0 36.0 41.0 31.0 41.0 22-23 37.13913206782438 39.0 36.0 41.0 31.0 41.0 24-25 37.19001997892822 39.0 36.5 41.0 31.0 41.0 26-27 37.089079706692786 39.0 36.0 41.0 31.0 41.0 28-29 36.943044935081744 39.0 36.0 41.0 30.5 41.0 30-31 36.72689478860851 39.0 36.0 41.0 30.0 41.0 32-33 36.53578169811341 39.0 35.5 41.0 30.0 41.0 34-35 36.34722035070818 39.0 35.0 40.5 30.0 41.0 36-37 36.136806055853995 39.0 35.0 40.0 29.5 41.0 38-39 35.924801828766896 38.0 35.0 40.0 29.0 41.0 40-41 35.69964127054524 38.0 35.0 40.0 27.5 41.0 42-43 35.46584218662632 38.0 35.0 40.0 27.0 41.0 44-45 35.18782473715985 38.0 34.0 40.0 26.0 41.0 46-47 34.995133916113964 38.0 34.0 40.0 25.5 41.0 48-49 34.82399179622554 38.0 34.0 40.0 24.0 41.0 50-51 33.85339121882072 37.5 33.0 39.5 23.5 40.5 52-53 33.94323300013899 37.5 33.0 39.5 23.0 40.5 54-55 34.37262610968994 38.0 34.0 40.0 23.5 41.0 56-57 34.26937812800732 38.0 34.0 40.0 23.0 41.0 58-59 34.02796307240479 38.0 33.0 40.0 23.0 41.0 60-61 33.73372587382612 37.0 33.0 40.0 22.0 41.0 62-63 33.35321402917582 37.0 33.0 40.0 21.0 41.0 64-65 32.99040841682644 36.0 32.0 39.0 20.0 41.0 66-67 32.526728580477524 36.0 31.5 39.0 18.0 41.0 68-69 32.146545650736925 35.0 31.0 39.0 17.5 40.0 70-71 31.752705855640308 35.0 31.0 38.0 14.0 40.0 72-73 31.34419645557049 35.0 31.0 37.0 12.0 39.5 74-75 30.914344721923662 35.0 30.5 37.0 8.0 39.0 76-77 30.448917922997836 34.5 30.0 36.0 7.0 39.0 78-79 29.95301317887772 34.0 29.5 36.0 4.0 38.5 80-81 29.498122267221085 34.0 29.0 35.0 2.0 37.0 82-83 29.088223201657733 34.0 29.0 35.0 2.0 37.0 84-85 28.718951758792905 34.0 29.0 35.0 2.0 36.0 86-87 28.350998097598605 34.0 27.5 35.0 2.0 36.0 88-89 27.93858734229326 34.0 27.0 35.0 2.0 36.0 90-91 27.525271009970368 33.0 26.0 35.0 2.0 35.0 92-93 27.070232882366234 33.0 25.5 35.0 2.0 35.0 94-95 26.565333376481313 33.0 25.0 35.0 2.0 35.0 96-97 25.95680551473592 33.0 23.5 35.0 2.0 35.0 98-99 25.18379048969064 32.0 19.0 35.0 2.0 35.0 100-101 22.287375343901758 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.392610184089353 1101 2 0.40718863860402266 1101 3 0.4457255403575111 1101 4 0.4287124999712546 1101 5 0.445581893138808 1101 6 0.5591058704598879 1101 7 0.6351770827700562 1101 8 0.5705802860132181 1101 9 0.5707875970830614 1101 10-11 0.5903570742280593 1101 12-13 0.724187987616105 1101 14-15 0.7921234729039526 1101 16-17 0.761555327067434 1101 18-19 0.7411687620382068 1101 20-21 0.6452336356666422 1101 22-23 0.6886506112106616 1101 24-25 0.7250841167847852 1101 26-27 0.6908956456072985 1101 28-29 0.6367796372773569 1101 30-31 0.5272943882459984 1101 32-33 0.5322211763628033 1101 34-35 0.5528219055417267 1101 36-37 0.5477726402716314 1101 38-39 0.596268845965497 1101 40-41 0.5140492309318674 1101 42-43 0.7822325782586859 1101 44-45 0.6586006914724791 1101 46-47 0.649728353808321 1101 48-49 0.6329150032402424 1101 50-51 0.5806902526890738 1101 52-53 0.6644706205563793 1101 54-55 0.5890027699771565 1101 56-57 0.5509861430127359 1101 58-59 0.5674106665748084 1101 60-61 0.5080060812235345 1101 62-63 0.7453930259345682 1101 64-65 0.5864242788387912 1101 66-67 0.5672091134105486 1101 68-69 0.5090881427137433 1101 70-71 0.39003074931381576 1101 72-73 0.4428813481427909 1101 74-75 0.4672065830912544 1101 76-77 0.5139463754528819 1101 78-79 0.17500533595841716 1101 80-81 0.26327788521967577 1101 82-83 0.2386358454117783 1101 84-85 0.2657287689494723 1101 86-87 0.21042691437584082 1101 88-89 0.21341744391107653 1101 90-91 0.3886748420933017 1101 92-93 0.3769653855068533 1101 94-95 0.4076898973360734 1101 96-97 0.2639783372277833 1101 98-99 -0.03834227668728474 1101 100-101 0.20007099801933137 1102 1 0.31741582062968376 1102 2 0.32879537653728974 1102 3 0.2845152228971948 1102 4 0.20583227645327185 1102 5 0.23035473084532043 1102 6 0.4500497480109118 1102 7 0.3536638822517588 1102 8 0.31331190246932294 1102 9 0.3387560940056389 1102 10-11 0.3920860991713724 1102 12-13 0.4309370562911923 1102 14-15 0.2957070491916056 1102 16-17 0.2923396646119798 1102 18-19 0.25050731888951816 1102 20-21 0.2870126687084351 1102 22-23 0.2535143543269527 1102 24-25 0.3673338738304608 1102 26-27 0.006351589889767695 1102 28-29 -0.04880203586809273 1102 30-31 0.3415153147118204 1102 32-33 0.17670813781405315 1102 34-35 0.1514006227428908 1102 36-37 -0.056611672977474825 1102 38-39 -0.020379599126819414 1102 40-41 -0.05705033984882846 1102 42-43 -0.09315716097033544 1102 44-45 0.10811174589425576 1102 46-47 0.13820320803572628 1102 48-49 0.10626088921367227 1102 50-51 0.0680060123262578 1102 52-53 0.28549224349126945 1102 54-55 0.177276174577095 1102 56-57 0.06007162244259945 1102 58-59 0.05637142945138862 1102 60-61 0.1125321178286498 1102 62-63 0.011579857810183114 1102 64-65 -0.046385690386998135 1102 66-67 -0.007291817914371279 1102 68-69 -0.01751535582852881 1102 70-71 0.03370786301644202 1102 72-73 -0.2247419511769344 1102 74-75 -0.1229395944273719 1102 76-77 -0.09288266568738379 1102 78-79 -0.12681985840503884 1102 80-81 -0.015173078499145731 1102 82-83 0.1485655911195849 1102 84-85 -0.3129732475714242 1102 86-87 -0.014594344127562664 1102 88-89 -0.12193544724538796 1102 90-91 -0.27982916665305524 1102 92-93 -0.282022912193181 1102 94-95 -0.35599652961566264 1102 96-97 -0.06340767670159764 1102 98-99 -0.15200946975498653 1102 100-101 -0.08812344642511505 1103 1 0.2925242734364346 1103 2 0.2708770693027631 1103 3 0.31593397596543227 1103 4 0.39663407581808485 1103 5 0.4696522171446986 1103 6 0.3893863073769239 1103 7 0.495914891639309 1103 8 0.4487201158283014 1103 9 0.25622676415882495 1103 10-11 0.4828358011139997 1103 12-13 0.1339792451844275 1103 14-15 0.5376745283776856 1103 16-17 0.5775480042070242 1103 18-19 0.7148356127991349 1103 20-21 0.7579683558433672 1103 22-23 0.6331743184598793 1103 24-25 0.6246300176193529 1103 26-27 0.4178736361162123 1103 28-29 0.5040042118150723 1103 30-31 0.5007029956752191 1103 32-33 0.47339794775864874 1103 34-35 0.5943965250802634 1103 36-37 0.7827203779577658 1103 38-39 0.7602201223523437 1103 40-41 0.5245247054859519 1103 42-43 0.724905115486635 1103 44-45 0.8239403453752843 1103 46-47 0.9800624848834332 1103 48-49 0.877550701162022 1103 50-51 0.7278173595455613 1103 52-53 1.097352922961882 1103 54-55 0.9780962080396662 1103 56-57 0.8414127303143246 1103 58-59 0.8480393484599276 1103 60-61 0.9675334703578784 1103 62-63 1.0171928541132687 1103 64-65 1.0451079026319547 1103 66-67 1.0130061392655634 1103 68-69 0.8621195614668125 1103 70-71 0.8252755946746433 1103 72-73 0.7043028626248713 1103 74-75 0.7966074585617164 1103 76-77 1.0500646457850706 1103 78-79 0.9605585391984768 1103 80-81 0.8226661809159594 1103 82-83 0.69559102868784 1103 84-85 0.7031443924153535 1103 86-87 0.8552247175177143 1103 88-89 0.8293584723843246 1103 90-91 0.8127589731684068 1103 92-93 0.7324641786667279 1103 94-95 0.7172955265293304 1103 96-97 0.9418721966615884 1103 98-99 0.8126820032996243 1103 100-101 0.7947926065940294 1104 1 0.3254810429203303 1104 2 0.24587940787086282 1104 3 0.29302616050709673 1104 4 0.41670442864818824 1104 5 0.37180797810750477 1104 6 0.40889177817600597 1104 7 0.38847345917389475 1104 8 0.3385238863977307 1104 9 0.37589401311081616 1104 10-11 0.548814965016085 1104 12-13 0.584406314853446 1104 14-15 0.5004260332190711 1104 16-17 0.3968078709702709 1104 18-19 0.34754119468925637 1104 20-21 0.23802417692007083 1104 22-23 0.39308712793945944 1104 24-25 0.416219729079927 1104 26-27 0.2103280739208202 1104 28-29 0.2773284374227174 1104 30-31 0.36243307198142105 1104 32-33 0.3891449589235876 1104 34-35 0.3565735760770039 1104 36-37 0.11255020996518539 1104 38-39 0.16642033713804238 1104 40-41 0.10293526232620565 1104 42-43 0.17887883215659883 1104 44-45 0.3035317159502213 1104 46-47 -0.013846723563837315 1104 48-49 0.29199319015262404 1104 50-51 0.2677191324339745 1104 52-53 0.32641802507210116 1104 54-55 0.2425806462285962 1104 56-57 0.06973094090727017 1104 58-59 0.3117803032773523 1104 60-61 0.4026945850100816 1104 62-63 0.3583058239932342 1104 64-65 0.3078980572965122 1104 66-67 0.5156470896596304 1104 68-69 0.4847598737399821 1104 70-71 0.3698062821266461 1104 72-73 0.31750877481508866 1104 74-75 0.4201465786364942 1104 76-77 0.45414927221966295 1104 78-79 0.30111206557788606 1104 80-81 0.4455505019226855 1104 82-83 0.47047720024519535 1104 84-85 0.3523910834321349 1104 86-87 0.2522265644426618 1104 88-89 0.2949648431514227 1104 90-91 0.20493684357040465 1104 92-93 -0.007169438412869766 1104 94-95 0.3267539286600716 1104 96-97 0.3333213039811973 1104 98-99 0.44609060589460015 1104 100-101 0.6948124753586669 1105 1 0.3311436451637988 1105 2 0.2683529751781961 1105 3 0.27663785436408617 1105 4 0.4185176060673399 1105 5 0.45728927728471547 1105 6 0.41594489891777187 1105 7 0.4194845899259292 1105 8 0.5464676727612598 1105 9 0.5225838086128789 1105 10-11 0.568039341869337 1105 12-13 0.43614989296940365 1105 14-15 0.40175462044493315 1105 16-17 0.3846355141845095 1105 18-19 0.3842842701639597 1105 20-21 0.4303347196919063 1105 22-23 0.0954354531901771 1105 24-25 0.16182565393616244 1105 26-27 0.05344236573598238 1105 28-29 -0.023573528454910786 1105 30-31 0.08997032797110904 1105 32-33 -0.18729320042616138 1105 34-35 0.00593470803977425 1105 36-37 0.041805104847128405 1105 38-39 0.03591338858135629 1105 40-41 0.03148315743342067 1105 42-43 0.042631133483055805 1105 44-45 0.14147650759624497 1105 46-47 0.35064449709126677 1105 48-49 0.13856946944798665 1105 50-51 0.18815615882300563 1105 52-53 0.4192232108622491 1105 54-55 0.2551312680728657 1105 56-57 -0.11270692052664089 1105 58-59 0.11526811991382147 1105 60-61 0.22572964295324027 1105 62-63 0.08535071169144004 1105 64-65 0.14044184028463036 1105 66-67 0.49435491273872145 1105 68-69 0.5739078858121758 1105 70-71 0.45009790210637846 1105 72-73 0.29257153159322513 1105 74-75 0.3960271363995105 1105 76-77 0.32069728619969595 1105 78-79 0.2996826565886046 1105 80-81 0.35535978442247895 1105 82-83 0.2659711865278567 1105 84-85 0.2393987976912193 1105 86-87 -0.04629390919949117 1105 88-89 -0.06106072477815516 1105 90-91 0.03658064189459154 1105 92-93 0.022934369846737468 1105 94-95 0.1322185562909617 1105 96-97 -0.05756518641615216 1105 98-99 0.16529575763722448 1105 100-101 0.28449027864762755 1106 1 0.4088471522494004 1106 2 0.3958004541389144 1106 3 0.48541688533731886 1106 4 0.3464093864181379 1106 5 0.28765888665810735 1106 6 0.510157222953552 1106 7 0.48860703956540164 1106 8 0.7023134033232523 1106 9 0.6768708834548036 1106 10-11 0.7393720549738063 1106 12-13 0.6027790813514287 1106 14-15 0.6253335320755511 1106 16-17 0.7561107182574389 1106 18-19 0.6554359851996594 1106 20-21 0.33279087009887576 1106 22-23 0.6033786449290801 1106 24-25 0.4930117020200697 1106 26-27 0.5006953544454618 1106 28-29 0.47709703889282906 1106 30-31 0.5982181226312662 1106 32-33 0.5416046881501657 1106 34-35 0.481587050653026 1106 36-37 0.5953814150438674 1106 38-39 0.48210816690097147 1106 40-41 0.469730702238337 1106 42-43 0.4115574467662455 1106 44-45 0.34024957143866885 1106 46-47 0.46468062909713126 1106 48-49 0.3575058504272448 1106 50-51 0.19271417961684278 1106 52-53 0.17370737738929165 1106 54-55 0.45504484755769425 1106 56-57 0.5465761566315948 1106 58-59 0.6641324544023703 1106 60-61 0.6837407365254933 1106 62-63 0.6860541276533709 1106 64-65 0.7139147104157644 1106 66-67 0.668107547241668 1106 68-69 0.817801864889006 1106 70-71 0.6663321722498523 1106 72-73 0.3638846237133748 1106 74-75 0.5454299089107337 1106 76-77 0.6185034102983771 1106 78-79 0.4309709248566982 1106 80-81 0.606254486609739 1106 82-83 0.5181702799942904 1106 84-85 0.5361966824462314 1106 86-87 0.5147247799717825 1106 88-89 0.4279629144661641 1106 90-91 0.48831118331617773 1106 92-93 0.5695974062638705 1106 94-95 0.5983187129648861 1106 96-97 0.6173158384018222 1106 98-99 0.6750731807185559 1106 100-101 0.33530568854248344 1107 1 0.253069073527481 1107 2 0.33853372565845063 1107 3 0.276111861552657 1107 4 0.29650888323043745 1107 5 0.17634993564993096 1107 6 0.2584436938245318 1107 7 0.4239928541766602 1107 8 0.38896401205265363 1107 9 0.5016229214088668 1107 10-11 0.4007836958571005 1107 12-13 0.3113102139576185 1107 14-15 0.27386469880607933 1107 16-17 0.3040956275013329 1107 18-19 0.255975052201336 1107 20-21 0.3822810281530451 1107 22-23 0.32705805752252104 1107 24-25 0.35943491592797727 1107 26-27 0.3795353171621869 1107 28-29 0.4803348289618725 1107 30-31 0.5173806188166523 1107 32-33 0.3004988305617431 1107 34-35 0.49765425024430954 1107 36-37 0.35442753262072557 1107 38-39 0.2880580616517747 1107 40-41 0.4950738711801179 1107 42-43 0.5533190765835059 1107 44-45 0.43324179791506623 1107 46-47 0.19667029234844335 1107 48-49 0.2615725937274149 1107 50-51 0.15750651841104713 1107 52-53 0.3341495215285377 1107 54-55 0.2754557825547366 1107 56-57 0.1326468710294293 1107 58-59 0.3126966486146827 1107 60-61 0.44433122402575265 1107 62-63 0.5584027886631944 1107 64-65 0.41487024055680877 1107 66-67 0.3374494399333088 1107 68-69 0.39755823663367096 1107 70-71 0.24595326537906814 1107 72-73 0.24556970289646074 1107 74-75 0.31034212162039054 1107 76-77 0.38617786608835303 1107 78-79 0.17149106260201563 1107 80-81 0.2630264366010131 1107 82-83 0.18431350373000654 1107 84-85 0.027471333622401062 1107 86-87 0.0030538916373323843 1107 88-89 0.02799511321409298 1107 90-91 0.0221992077219042 1107 92-93 0.18832657213836868 1107 94-95 0.23890865935068106 1107 96-97 0.36496199049301126 1107 98-99 0.5419899787639153 1107 100-101 0.6741932449573227 1108 1 0.2317871790882684 1108 2 0.2716519277248466 1108 3 0.29067598248261106 1108 4 0.4216933110171297 1108 5 0.27485863808335864 1108 6 0.21981336121875472 1108 7 0.33845723425226737 1108 8 0.14841876539279042 1108 9 0.31887821460860977 1108 10-11 0.4123337720609186 1108 12-13 0.1854714640965014 1108 14-15 0.42140429482176245 1108 16-17 0.4819542362505942 1108 18-19 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-2.015793009463504 2316 84-85 -1.7270079090550645 2316 86-87 -1.5109095368713277 2316 88-89 -1.8452914563156817 2316 90-91 -1.9442687520110091 2316 92-93 -2.7927129024750066 2316 94-95 -2.8219019393986216 2316 96-97 -3.263480562998975 2316 98-99 -4.022717276687285 2316 100-101 -4.978713015586113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4277.0 3 1786.0 4 1858.0 5 1933.0 6 2210.0 7 2807.0 8 3450.0 9 4224.0 10 5510.0 11 6988.0 12 8630.0 13 10308.0 14 11180.0 15 13552.0 16 15798.0 17 19659.0 18 16230.0 19 12896.0 20 13134.0 21 14010.0 22 15588.0 23 17551.0 24 19948.0 25 22761.0 26 26684.0 27 31211.0 28 37585.0 29 44941.0 30 53590.0 31 66193.0 32 81642.0 33 104677.0 34 137267.0 35 185347.0 36 256321.0 37 331019.0 38 248546.0 39 33669.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.05903919056222 18.116316888312337 13.998721326000855 22.825922595124588 2 15.703611503287116 19.924203475943916 40.160856714008986 24.21132830675998 3 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46-47 22.846659915001162 26.777630589819346 27.796693523016586 22.579015972162907 48-49 22.885845049239432 26.800887864176303 27.664932070645044 22.64833501593922 50-51 22.749957690265155 27.01515033351743 27.712160109586193 22.522731866631226 52-53 23.04087579429869 26.98393961539431 27.405416947956606 22.569767642350396 54-55 22.604229419718138 26.87816152691819 27.565397798307945 22.952211255055722 56-57 22.802313669547168 27.253443156419916 27.24540589749126 22.698837276541656 58-59 23.047863652798384 26.77778694436656 27.316764679901397 22.857584722933662 60-61 22.738248553326322 26.818113694028018 27.89677587068999 22.54686188195567 62-63 22.712553442410048 26.924735791971553 27.79859446262625 22.564116302992137 64-65 22.50209486703861 27.088839073627113 27.59380879586857 22.815257263465703 66-67 22.511776106078475 27.232963280834426 27.308256075091165 22.947004537995934 68-69 22.574778857110406 27.060077306469786 27.580938005688388 22.78420583073142 70-71 22.668409802538374 27.032831359035566 27.338915189857964 22.959843648568096 72-73 22.66806422841486 26.898686323791814 27.500025202802973 22.933224244990345 74-75 22.54946964213315 27.470802191480736 27.17683407026578 22.802894096120333 76-77 22.773982915824817 27.132238520450464 27.172275824923688 22.92150273880103 78-79 22.685960623491027 27.288786455835524 27.199080862691787 22.826172057981662 80-81 22.815497124459508 27.433776557336664 27.033982984615644 22.716743333588184 82-83 22.72850314160969 27.433195015461244 27.108286015458056 22.73001582747101 84-85 22.669062881538586 27.368265222145993 27.019577754678746 22.943094141636674 86-87 22.777188347913434 27.32072422137553 27.065788066844267 22.836299363866775 88-89 22.827233576502902 27.225325222492724 26.915942004376625 23.03149919662775 90-91 22.716729429103744 27.211503530768542 27.11698173489688 22.954785305230835 92-93 22.827695044192186 27.199341362358954 27.133939260152317 22.839024333296543 94-95 22.563587762201028 27.0814785023542 27.265670514103434 23.089263221341337 96-97 22.538693842227765 27.0062179165244 27.337316599054084 23.117771642193752 98-99 22.425571598341666 27.204057834706873 26.94167220877902 23.428698358172433 100-101 22.580182789579634 27.018372391298804 27.04082824404787 23.360616575073685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 130.0 1 111.0 2 220.5 3 331.0 4 260.5 5 232.5 6 393.0 7 751.5 8 1180.0 9 1505.0 10 1515.5 11 1409.5 12 1521.5 13 1674.0 14 1826.5 15 2011.5 16 2127.0 17 2254.5 18 2392.5 19 2594.5 20 2930.5 21 3355.0 22 4038.0 23 4943.0 24 5919.0 25 7256.0 26 9166.5 27 11673.5 28 14109.0 29 16969.5 30 20757.0 31 25070.5 32 29936.0 33 33274.0 34 36041.0 35 39185.5 36 42839.5 37 46538.0 38 49073.0 39 50700.5 40 52285.5 41 55730.0 42 58467.5 43 60717.0 44 64008.0 45 67122.5 46 68922.5 47 71818.5 48 80292.0 49 80003.5 50 71586.5 51 67689.0 52 65377.0 53 64602.0 54 63660.0 55 59722.5 56 54091.0 57 48948.0 58 45481.5 59 41213.0 60 34735.0 61 28894.0 62 24003.5 63 18701.5 64 13813.0 65 10522.0 66 7935.5 67 6365.0 68 4953.5 69 3502.0 70 2633.5 71 2068.5 72 1655.0 73 1414.0 74 1159.0 75 906.5 76 657.0 77 394.5 78 260.5 79 179.0 80 106.5 81 71.0 82 45.0 83 27.5 84 22.0 85 16.5 86 13.0 87 11.0 88 8.0 89 5.5 90 4.0 91 3.0 92 1.5 93 1.0 94 1.5 95 2.0 96 2.0 97 1.0 98 0.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011724225007506688 2 0.010769310753501618 3 0.013687104307405997 4 0.01809032003420715 5 0.029177935539043794 6 0.04588893498413251 7 0.006153891859143781 8 0.02530522773113434 9 0.03411165918473665 10-11 0.03848834951559322 12-13 0.0286209022242075 14-15 0.02766598797020243 16-17 0.027825140345869942 18-19 0.021777350070504502 20-21 0.0534486728283393 22-23 0.012201682134509222 24-25 0.0025729634066247705 26-27 0.0015915237566751159 28-29 0.0 30-31 0.002811691970126038 32-33 0.0020159300917884804 34-35 0.0 36-37 3.1830475133502316E-4 38-39 2.6525395944585266E-5 40-41 0.0015119475688413601 42-43 0.004164487163299886 44-45 0.002042455487733065 46-47 0.0010079650458942402 48-49 6.896602945592169E-4 50-51 0.00408491097546613 52-53 0.0036339792444081815 54-55 0.006233468046977538 56-57 7.957618783375579E-4 58-59 0.0064456712145342186 60-61 0.0031034713255164758 62-63 6.896602945592169E-4 64-65 0.0011405920256171663 66-67 0.018222947013930076 68-69 0.04228148113566891 70-71 0.05437706168639979 72-73 0.014588967769521897 74-75 0.031724373549723975 76-77 0.026180565797305655 78-79 0.05546460292012779 80-81 0.08031889892020418 82-83 0.048806728538036886 84-85 0.018408624785542175 86-87 0.021034638984056116 88-89 0.022811840512343327 90-91 0.04055733039927087 92-93 0.025994888025693563 94-95 0.014217612226297702 96-97 0.018647353349043442 98-99 0.04917808408126108 100-101 0.06843552153702998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884986.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.1816564939364 #Duplication Level Percentage of deduplicated Percentage of total 1 81.57245251026669 49.907377688510756 2 11.136472013327737 13.626956105475077 3 3.108359307170286 5.705237141730677 4 1.2947474506814105 3.168591750959597 5 0.7064094292948502 2.1609649523597585 6 0.4339795000479761 1.5930950818407317 7 0.29380076523980814 1.2582652247590314 8 0.21066409670621455 1.0311022720228014 9 0.1636415657867322 0.9010675859484372 >10 0.9646070435629286 10.997238461707246 >50 0.07560950864324288 3.1756829619570652 >100 0.036036996722095996 4.0928762589738295 >500 0.0023495929418078936 0.9999281820847331 >1k 6.961756864615979E-4 0.7825964724169903 >5k 1.7404392161539948E-4 0.5990198592532523 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6174 0.3275355891237388 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5077 0.26933887042131877 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3063 0.16249457555652932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2857 0.1515661124273602 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1929 0.10233497755420995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.305079188917053E-5 2 0.0 0.0 0.0 0.0 5.305079188917053E-5 3 0.0 0.0 0.0 0.0 5.305079188917053E-5 4 0.0 0.0 0.0 0.0 5.305079188917053E-5 5 0.0 0.0 0.0 0.0 5.305079188917053E-5 6 0.0 0.0 0.0 0.0 5.305079188917053E-5 7 0.0 0.0 0.0 0.0 5.305079188917053E-5 8 0.0 0.0 0.0 0.0 5.305079188917053E-5 9 0.0 0.0 0.0 0.0 5.305079188917053E-5 10-11 5.305079188917053E-5 0.0 0.0 0.0 5.305079188917053E-5 12-13 5.305079188917053E-5 0.0 0.0 0.0 5.305079188917053E-5 14-15 1.0610158377834106E-4 0.0 0.0 0.0 5.305079188917053E-5 16-17 1.0610158377834106E-4 0.0 0.0 0.0 5.305079188917053E-5 18-19 2.1220316755668213E-4 0.0 0.0 0.0 5.305079188917053E-5 20-21 3.1830475133502316E-4 0.0 0.0 0.0 5.305079188917053E-5 22-23 3.1830475133502316E-4 2.6525395944585266E-5 0.0 7.95761878337558E-5 7.95761878337558E-5 24-25 3.1830475133502316E-4 5.305079188917053E-5 0.0 2.6525395944585264E-4 1.0610158377834106E-4 26-27 3.1830475133502316E-4 5.305079188917053E-5 0.0 4.5093173105794954E-4 1.5915237566751158E-4 28-29 3.1830475133502316E-4 5.305079188917053E-5 0.0 6.896602945592169E-4 1.5915237566751158E-4 30-31 3.1830475133502316E-4 5.305079188917053E-5 0.0 0.0014058459850630192 1.5915237566751158E-4 32-33 3.4483014727960845E-4 5.305079188917053E-5 0.0 0.0023607602390680886 1.5915237566751158E-4 34-35 3.7135554322419373E-4 7.95761878337558E-5 0.0 0.00347482686874067 1.5915237566751158E-4 36-37 4.2440633511336425E-4 1.0610158377834106E-4 0.0 0.004960249041637445 1.5915237566751158E-4 38-39 5.305079188917053E-4 1.0610158377834106E-4 0.0 0.0060477902753654405 1.5915237566751158E-4 40-41 5.305079188917053E-4 1.0610158377834106E-4 0.0 0.007771941011763483 1.5915237566751158E-4 42-43 6.366095026700464E-4 1.0610158377834106E-4 0.0 0.011034564712947469 1.5915237566751158E-4 44-45 6.896602945592169E-4 1.0610158377834106E-4 0.0 0.01591523756675116 1.5915237566751158E-4 46-47 7.161856905038022E-4 1.0610158377834106E-4 0.0 0.029788019645769253 1.5915237566751158E-4 48-49 7.427110864483875E-4 1.0610158377834106E-4 0.0 0.055040196585014425 1.5915237566751158E-4 50-51 7.427110864483875E-4 1.0610158377834106E-4 0.0 0.07188382300982607 1.5915237566751158E-4 52-53 7.427110864483875E-4 1.0610158377834106E-4 0.0 0.09026592239942366 1.5915237566751158E-4 54-55 7.427110864483875E-4 1.0610158377834106E-4 0.0 0.11779928338990317 1.5915237566751158E-4 56-57 8.488126702267285E-4 1.0610158377834106E-4 0.0 0.1380912112875109 1.5915237566751158E-4 58-59 8.488126702267285E-4 1.5915237566751158E-4 0.0 0.16026644229718418 1.5915237566751158E-4 60-61 8.488126702267285E-4 1.5915237566751158E-4 0.0 0.19838343626955318 1.5915237566751158E-4 62-63 8.488126702267285E-4 1.5915237566751158E-4 0.0 0.2239008671682442 1.5915237566751158E-4 64-65 8.488126702267285E-4 2.1220316755668213E-4 0.0 0.25395414077345935 1.5915237566751158E-4 66-67 8.488126702267285E-4 2.3872856350126738E-4 0.0 0.3180660227715219 1.5915237566751158E-4 68-69 8.488126702267285E-4 2.6525395944585264E-4 0.0 0.3939551805689804 1.5915237566751158E-4 70-71 8.753380661713137E-4 2.6525395944585264E-4 0.0 0.4548840150536927 1.5915237566751158E-4 72-73 9.549142540050694E-4 2.6525395944585264E-4 0.0 0.5261577539567933 1.5915237566751158E-4 74-75 0.00100796504589424 2.6525395944585264E-4 0.0 0.5841157440957121 1.5915237566751158E-4 76-77 0.00100796504589424 2.6525395944585264E-4 0.0 0.6303760346230688 1.5915237566751158E-4 78-79 0.0010344904418388254 2.6525395944585264E-4 0.0 0.6945144420170759 1.5915237566751158E-4 80-81 0.0011671174215617517 2.6525395944585264E-4 0.0 0.8048335637506061 1.5915237566751158E-4 82-83 0.001220168213450922 2.6525395944585264E-4 0.0 0.9086539634777129 1.5915237566751158E-4 84-85 0.001220168213450922 2.6525395944585264E-4 0.0 1.0253391802379435 1.5915237566751158E-4 86-87 0.001220168213450922 2.6525395944585264E-4 0.0 1.2422108174808724 1.5915237566751158E-4 88-89 0.0012466936093955073 2.6525395944585264E-4 0.0 1.5164834115478842 1.5915237566751158E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1880 0.0 27.543118 2 GTACACG 335 0.0 26.942022 1 GTACCTG 1970 0.0 26.524538 1 GTACATG 8515 0.0 25.383303 1 GTATCAA 12430 0.0 24.649607 1 TACATGG 8670 0.0 24.328106 2 ACATGGG 8600 0.0 22.924805 3 GAGTACT 6195 0.0 22.547705 12-13 GGTATCA 9245 0.0 21.426466 1 ATCAACG 14165 0.0 21.363815 3 TCAACGC 14220 0.0 21.348568 4 TATCAAC 14245 0.0 21.34332 2 CAACGCA 14235 0.0 21.22595 5 AACGCAG 14445 0.0 20.950815 6 GTATAGG 480 0.0 20.78258 1 AGTACTT 6645 0.0 19.769537 12-13 GTGTAGC 1020 0.0 19.560074 1 GTACTTT 7015 0.0 19.540325 14-15 ACGCAGA 15795 0.0 19.160656 7 CATGGGA 4710 0.0 19.063753 4 >>END_MODULE