FastQCFastQC Report
Thu 26 May 2016
SRR936443_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936443_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1884986
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.3688091052135135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62800.3331589730639909No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37160.19713674266015768No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34520.18313133360141667No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20620.10939073287546963No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGG600.003953614331.6633516
TATACCG902.4155057E-531.663355
TACCTGG17750.029.437442
GTACCTG19100.028.3583471
TATCACG1109.4779825E-525.9098152
GGTATCA101500.024.575521
GTATCAA134150.024.048531
GTACATG80450.022.6194571
GAGTACT82450.022.38207612-13
TACATGG82550.021.463352
ACATGGG82000.021.0252383
GTACTTT89750.020.50947214-15
ATCAACG157300.020.380893
TATCAAC159500.020.1917862
TCAACGC159800.020.062044
CAACGCA161750.019.8495455
CCGTACG600.004762514519.79379578-79
AACGCAG164750.019.4880966
AGTACTT87100.019.08753612-13
GTACACG3851.2732926E-1118.511431
Produced by FastQC (version 0.11.5)