##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936443_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1884986 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.941811769424284 31.0 31.0 34.0 30.0 34.0 2 32.05331604584862 33.0 31.0 34.0 30.0 34.0 3 32.179776932030265 33.0 31.0 34.0 30.0 34.0 4 35.909302244154595 37.0 35.0 37.0 35.0 37.0 5 35.77618136155918 37.0 35.0 37.0 35.0 37.0 6 35.82330850202601 37.0 35.0 37.0 35.0 37.0 7 35.74973129773908 37.0 35.0 37.0 33.0 37.0 8 35.7199963288852 37.0 35.0 37.0 33.0 37.0 9 37.33484174418272 39.0 37.0 39.0 34.0 39.0 10-11 37.23286910353711 39.0 37.0 39.0 33.5 39.0 12-13 37.109972169554574 39.0 37.0 39.0 33.0 39.0 14-15 38.216218316740814 40.0 38.0 41.0 33.0 41.0 16-17 38.18928018563533 40.0 37.5 41.0 33.0 41.0 18-19 38.335113894745106 40.0 38.0 41.0 33.5 41.0 20-21 38.34620522380538 40.0 38.0 41.0 34.0 41.0 22-23 38.24618750484089 40.0 38.0 41.0 33.5 41.0 24-25 38.13438322618842 40.0 37.0 41.0 33.0 41.0 26-27 37.93162044704842 40.0 37.0 41.0 33.0 41.0 28-29 37.94609535561537 40.0 37.0 41.0 33.0 41.0 30-31 37.82077400044351 40.0 37.0 41.0 33.0 41.0 32-33 37.62775240771019 40.0 37.0 41.0 33.0 41.0 34-35 37.44265899057075 40.0 36.0 41.0 32.0 41.0 36-37 37.25592205989859 39.0 36.0 41.0 31.5 41.0 38-39 37.05524497264171 39.0 36.0 41.0 31.0 41.0 40-41 36.82966345638641 39.0 35.5 41.0 31.0 41.0 42-43 36.57254297909905 39.0 35.0 40.5 30.0 41.0 44-45 36.285456762013084 39.0 35.0 40.0 30.0 41.0 46-47 35.899150179364725 39.0 35.0 40.0 29.5 41.0 48-49 35.497481944162985 38.0 35.0 40.0 27.5 41.0 50-51 35.18347722476453 38.0 34.0 40.0 27.0 41.0 52-53 34.904828205620625 38.0 34.0 40.0 26.0 41.0 54-55 34.578392359412746 38.0 34.0 40.0 26.0 41.0 56-57 34.23901477252351 38.0 33.0 40.0 24.5 41.0 58-59 33.866699009966126 37.0 33.0 40.0 23.5 41.0 60-61 33.48565161757169 36.5 32.5 39.0 23.0 40.5 62-63 33.14782841888481 36.0 32.0 39.0 22.0 40.0 64-65 32.826853090686086 36.0 32.0 39.0 22.0 40.0 66-67 32.36766904369581 35.0 31.0 38.5 20.5 40.0 68-69 31.919111070320948 35.0 31.0 38.0 19.5 40.0 70-71 31.411836745737105 35.0 30.5 37.5 18.0 39.5 72-73 30.84461927038185 34.0 30.0 37.0 14.5 39.0 74-75 30.302395879863298 34.0 29.0 36.0 11.5 39.0 76-77 28.932837962722267 32.5 28.0 34.5 10.0 36.5 78-79 29.70746520133306 34.0 29.0 35.0 7.5 37.0 80-81 29.55097305762483 34.0 29.0 35.0 7.0 37.0 82-83 29.146219918874728 34.0 29.0 35.0 4.0 36.5 84-85 28.70367498750654 33.5 29.0 35.0 2.0 36.0 86-87 28.26508287064201 33.0 27.5 35.0 2.0 35.5 88-89 27.765564306578405 33.0 26.5 35.0 2.0 35.0 90-91 27.17624693233796 32.5 26.0 34.0 2.0 35.0 92-93 26.578560530423037 32.0 25.0 34.0 2.0 35.0 94-95 25.8367438803259 32.0 24.0 34.0 2.0 35.0 96-97 24.995804743377406 31.0 19.5 34.0 2.0 35.0 98-99 23.83468100028329 31.0 6.0 34.0 2.0 35.0 100-101 19.979721069546407 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.22189087030997356 1101 2 0.4141603890117125 1101 3 0.23062184033766897 1101 4 0.16476077932703248 1101 5 0.16370728236194765 1101 6 0.22571045905658593 1101 7 0.29507050543602276 1101 8 0.16988610641389812 1101 9 0.33434113241988683 1101 10-11 0.31008802651872713 1101 12-13 0.38658304102346364 1101 14-15 0.4410950240989209 1101 16-17 0.4022906777502868 1101 18-19 0.3956148794529497 1101 20-21 0.33314313490100034 1101 22-23 0.31593329269279735 1101 24-25 0.4437464094474777 1101 26-27 0.2993066537727529 1101 28-29 0.3533516098963858 1101 30-31 0.515841632193009 1101 32-33 0.42351196967766924 1101 34-35 0.5042373430357685 1101 36-37 0.5479144202870856 1101 38-39 0.45427016195231573 1101 40-41 0.48037402567563703 1101 42-43 0.6247269700101086 1101 44-45 0.7368436052210754 1101 46-47 0.6611362112125008 1101 48-49 0.6345882815050743 1101 50-51 0.528591268305 1101 52-53 0.5104898815064232 1101 54-55 0.7081927840548872 1101 56-57 0.762682810771139 1101 58-59 0.6290596865623854 1101 60-61 0.5702491351307799 1101 62-63 0.503858734311379 1101 64-65 0.6042782787225534 1101 66-67 0.6561590563907131 1101 68-69 0.6477235821182212 1101 70-71 0.564459068264874 1101 72-73 0.5317419697960943 1101 74-75 0.6993177868593712 1101 76-77 0.726081555479162 1101 78-79 0.7466519825685864 1101 80-81 0.6495541544812582 1101 82-83 0.6996887710606607 1101 84-85 0.43846481598043496 1101 86-87 0.5468690538950263 1101 88-89 0.3371399965421027 1101 90-91 0.38861144572767614 1101 92-93 0.3200706633250263 1101 94-95 0.3497654655442446 1101 96-97 0.6554973577498018 1101 98-99 0.3051558473587832 1101 100-101 0.6829822933586165 1102 1 0.19441678543792662 1102 2 0.2802216944862863 1102 3 0.28236098837783175 1102 4 0.14998105791465832 1102 5 0.12490545210603443 1102 6 0.08788449533776799 1102 7 0.1718926628704267 1102 8 0.09794125704558354 1102 9 0.1583713397409099 1102 10-11 0.22145019043190928 1102 12-13 0.3004911637963872 1102 14-15 0.34629728357413825 1102 16-17 0.31435639689509287 1102 18-19 0.32381681498905834 1102 20-21 0.16362175492044173 1102 22-23 0.1460373581285026 1102 24-25 0.22474333202602992 1102 26-27 0.19781754622497516 1102 28-29 0.3365523306608864 1102 30-31 0.4015619917654121 1102 32-33 0.2757704325866399 1102 34-35 0.28769923969919375 1102 36-37 0.25626293826570645 1102 38-39 0.23530757691829507 1102 40-41 0.33469253879517424 1102 42-43 0.3464126680988713 1102 44-45 0.38147735157676266 1102 46-47 0.1541563359290592 1102 48-49 0.24926778425854934 1102 50-51 0.14004150316085173 1102 52-53 0.30545161620030115 1102 54-55 0.114351676183162 1102 56-57 0.24945192317800036 1102 58-59 0.13057308954262226 1102 60-61 0.07445528187519557 1102 62-63 0.15105362417207857 1102 64-65 0.00970830787707655 1102 66-67 -0.0789397450346172 1102 68-69 -0.06059962811825059 1102 70-71 -0.33205757410636494 1102 72-73 -0.4583728261222717 1102 74-75 -0.20680061288147655 1102 76-77 -0.10454384944470974 1102 78-79 -0.07472597337569553 1102 80-81 -0.08451209106457824 1102 82-83 -0.1697334025706958 1102 84-85 -0.2507257411948167 1102 86-87 -0.1834060918775684 1102 88-89 -0.4761951589486664 1102 90-91 -0.5333546621440455 1102 92-93 -0.27833089968113 1102 94-95 -0.29294995395688517 1102 96-97 -0.4229060274137879 1102 98-99 -0.43951940369401754 1102 100-101 -0.358532526855182 1103 1 0.2001575206127093 1103 2 0.21468914862552424 1103 3 0.26191275017239235 1103 4 0.18920440557478457 1103 5 0.19638912657729435 1103 6 0.12614103506705732 1103 7 0.1935732054851158 1103 8 0.18435284671826935 1103 9 0.2078939470165153 1103 10-11 0.28519439208901076 1103 12-13 0.22748213691611596 1103 14-15 0.1990494300881238 1103 16-17 0.2347725751484262 1103 18-19 0.21526131620633748 1103 20-21 0.17202997829815558 1103 22-23 0.17021757513429492 1103 24-25 0.32562948913983547 1103 26-27 0.25061627985686386 1103 28-29 0.3209331227420762 1103 30-31 0.4238712100035187 1103 32-33 0.4558997744559363 1103 34-35 0.31728706893987635 1103 36-37 0.2924606279454025 1103 38-39 0.302914000931203 1103 40-41 0.29227571934279695 1103 42-43 0.47947148809225837 1103 44-45 0.36525701955588374 1103 46-47 0.38195982846009 1103 48-49 0.24050640047087057 1103 50-51 0.3428682892508377 1103 52-53 0.2870071250017645 1103 54-55 0.43778900398616116 1103 56-57 0.35835557300318044 1103 58-59 0.2320578660747401 1103 60-61 8.225887390267417E-4 1103 62-63 0.19272154318062462 1103 64-65 0.3017193685605548 1103 66-67 0.08968057496377924 1103 68-69 0.2052274685525255 1103 70-71 0.1361254281077784 1103 72-73 0.2275799054858325 1103 74-75 0.3500807552769807 1103 76-77 0.13613872697318996 1103 78-79 0.15634863614410577 1103 80-81 0.4983002680469575 1103 82-83 0.33253834559837614 1103 84-85 0.4105182851344189 1103 86-87 0.39814390661962307 1103 88-89 0.26133151593336024 1103 90-91 0.181589783970189 1103 92-93 0.0547269184387531 1103 94-95 0.0723456488203098 1103 96-97 0.2902901494369239 1103 98-99 0.21493943433309326 1103 100-101 0.3398459408794814 1104 1 0.24831316990435326 1104 2 0.2484000610006447 1104 3 0.25331987203680484 1104 4 0.16236634543333395 1104 5 0.10271442756369709 1104 6 0.1466421195100338 1104 7 0.08899679086895418 1104 8 0.11610146478767547 1104 9 0.18431900515440702 1104 10-11 0.21259617404164288 1104 12-13 0.2274466051837365 1104 14-15 0.21641792098293422 1104 16-17 0.1271150517391746 1104 18-19 0.21657562773551575 1104 20-21 0.3022338361741248 1104 22-23 0.10663354004925196 1104 24-25 0.14449053033560944 1104 26-27 0.19369988312259778 1104 28-29 0.22732767434973766 1104 30-31 0.35629891924372714 1104 32-33 0.37261303120025246 1104 34-35 0.41226375214692723 1104 36-37 0.32601128495613807 1104 38-39 0.3871278323469127 1104 40-41 0.344284748753644 1104 42-43 0.47385638667091 1104 44-45 0.5226908743355168 1104 46-47 0.45976380786908777 1104 48-49 0.5317027248944441 1104 50-51 0.530941374398644 1104 52-53 0.6457032264455762 1104 54-55 0.5876636639873638 1104 56-57 0.6699365973177578 1104 58-59 0.6901510882880117 1104 60-61 0.47064237034214784 1104 62-63 0.507310074842664 1104 64-65 0.5631962994020299 1104 66-67 0.632308501890229 1104 68-69 0.5947361082020173 1104 70-71 0.3726057852234419 1104 72-73 0.4798399774629729 1104 74-75 0.8162215523044942 1104 76-77 0.6394551428510873 1104 78-79 0.6211515546770947 1104 80-81 0.6896455356915467 1104 82-83 0.6181717784930747 1104 84-85 0.5582414624834477 1104 86-87 0.8613682010428079 1104 88-89 0.7032081280044338 1104 90-91 0.712096314195005 1104 92-93 0.4902524146321454 1104 94-95 0.6077298157353326 1104 96-97 0.8789878679371377 1104 98-99 0.7772769582354329 1104 100-101 0.8287342980009491 1105 1 0.07905294775893523 1105 2 0.21015236379466984 1105 3 0.2610143189883445 1105 4 0.17279486727915128 1105 5 0.14372481719505004 1105 6 0.17211759245142844 1105 7 0.15354399915541705 1105 8 0.17666569977136248 1105 9 0.16193129044037136 1105 10-11 0.17625851359085942 1105 12-13 0.13245548028729814 1105 14-15 0.18270646934766432 1105 16-17 0.29209511852808845 1105 18-19 0.21137530868673338 1105 20-21 0.16794460392377886 1105 22-23 0.17664743055857457 1105 24-25 0.2240036067128699 1105 26-27 0.27228105528198654 1105 28-29 0.15131033946372696 1105 30-31 0.2828094662715088 1105 32-33 0.3358692219427084 1105 34-35 0.13303002690017252 1105 36-37 0.22220009021824438 1105 38-39 0.22673574281953535 1105 40-41 0.22472547340823468 1105 42-43 0.36118182890349004 1105 44-45 0.19063835896459835 1105 46-47 0.2498582530595499 1105 48-49 0.3491397534848204 1105 50-51 0.5057623497281298 1105 52-53 0.49666789406762746 1105 54-55 0.5232742190133735 1105 56-57 0.4856701490248696 1105 58-59 0.6178250480224108 1105 60-61 0.5397276586564388 1105 62-63 0.5470271722031725 1105 64-65 0.383308333367367 1105 66-67 0.42242239232617607 1105 68-69 0.5134083868399166 1105 70-71 0.5537441172322985 1105 72-73 0.5429603450001252 1105 74-75 0.5373275685166945 1105 76-77 0.418524500739732 1105 78-79 0.43183741416795485 1105 80-81 0.6108134097978883 1105 82-83 0.4934851954923296 1105 84-85 0.6075391956080907 1105 86-87 0.5400883516737522 1105 88-89 0.6872160645997276 1105 90-91 0.6787756019266702 1105 92-93 0.6800170978103921 1105 94-95 0.767557259360629 1105 96-97 0.6045376730811007 1105 98-99 0.2578753728242127 1105 100-101 -0.07608599491048906 1106 1 0.11199489022168407 1106 2 0.23549797236852754 1106 3 0.1996947012374477 1106 4 0.1713236608899109 1106 5 0.18091576832043188 1106 6 0.18159298465064921 1106 7 0.22434635778879652 1106 8 0.16515766178160618 1106 9 0.3172030888395341 1106 10-11 0.13089435694043772 1106 12-13 0.32087057507061445 1106 14-15 0.3563044140679281 1106 16-17 0.28803754306446194 1106 18-19 0.3154852067751932 1106 20-21 0.2691286237711026 1106 22-23 0.3267960944209847 1106 24-25 0.3635536321393147 1106 26-27 0.2715009925536336 1106 28-29 0.29335965206789183 1106 30-31 0.2926537623705272 1106 32-33 0.16670694243994433 1106 34-35 0.13168374812640593 1106 36-37 0.22427131659590316 1106 38-39 0.22404685961362958 1106 40-41 0.09870841564464428 1106 42-43 0.3298874612282887 1106 44-45 0.237358715111192 1106 46-47 -0.02963789182121701 1106 48-49 0.08820344305292593 1106 50-51 0.20650963732914107 1106 52-53 0.2285838868600436 1106 54-55 0.24145919712898944 1106 56-57 0.27008219557436774 1106 58-59 0.1862398194636654 1106 60-61 0.2560276995151156 1106 62-63 0.3110124513208632 1106 64-65 0.4747171347383343 1106 66-67 0.28636005197632386 1106 68-69 0.41202631997865424 1106 70-71 0.18934900204903826 1106 72-73 0.2145179278124374 1106 74-75 0.12429078394775317 1106 76-77 -0.004959171955793096 1106 78-79 -0.08944914920562397 1106 80-81 0.12240448790851488 1106 82-83 0.29428994979645395 1106 84-85 0.19838251580197763 1106 86-87 0.2675782325368985 1106 88-89 0.25291292553618305 1106 90-91 0.4537486322590958 1106 92-93 0.3615949776858791 1106 94-95 0.4542726037052347 1106 96-97 0.5454516876079758 1106 98-99 0.45466392475147543 1106 100-101 0.03802068445470397 1107 1 0.059216486831090265 1107 2 0.11059754230440433 1107 3 0.07898888323325792 1107 4 0.0503748288684136 1107 5 8.423814996376677E-4 1107 6 0.10891643752641045 1107 7 -0.013011550915926762 1107 8 -0.012603801313893825 1107 9 0.004867921566912514 1107 10-11 0.03933364633527958 1107 12-13 0.026542088367897065 1107 14-15 0.08526261760691511 1107 16-17 0.08152376804522987 1107 18-19 0.17294554211016333 1107 20-21 -0.035894185909683074 1107 22-23 0.04516003240829747 1107 24-25 0.1081714932064699 1107 26-27 0.1256071494612243 1107 28-29 0.09218231374655517 1107 30-31 0.12057985100995694 1107 32-33 0.027972994283587127 1107 34-35 -0.09138264515559058 1107 36-37 0.1366672008110541 1107 38-39 0.13373753713768366 1107 40-41 0.14989845309366245 1107 42-43 0.14517755220376927 1107 44-45 0.1374990880024214 1107 46-47 0.2168814259650702 1107 48-49 0.2940470404988673 1107 50-51 0.3430882680853031 1107 52-53 0.28644401315908397 1107 54-55 0.09436637113677904 1107 56-57 0.11082672519965087 1107 58-59 0.08396284880767269 1107 60-61 0.043980907251388146 1107 62-63 -0.05048077109862703 1107 64-65 0.2086921030758262 1107 66-67 0.12873828416024935 1107 68-69 0.030922695096744945 1107 70-71 -0.09092330527277781 1107 72-73 -0.13633812989083793 1107 74-75 -0.10779966796569695 1107 76-77 -0.1937921538084737 1107 78-79 0.04077821149153138 1107 80-81 0.24040863352778175 1107 82-83 0.3852406879058279 1107 84-85 0.22155476764737259 1107 86-87 0.44035181464638384 1107 88-89 0.24965904828044216 1107 90-91 0.15344863060162695 1107 92-93 0.08700010667099178 1107 94-95 0.15416418581667202 1107 96-97 0.185168294752053 1107 98-99 0.1708222119438325 1107 100-101 0.23283090927557382 1108 1 -0.017576948781133694 1108 2 0.14119117468975872 1108 3 -0.006465479369516913 1108 4 -0.007948867697685102 1108 5 0.1046659146733191 1108 6 0.07541284000896553 1108 7 0.023942724034270668 1108 8 0.08247353484036779 1108 9 0.02489659737603489 1108 10-11 0.07037105906203323 1108 12-13 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1456.0 10 2386.0 11 3737.0 12 5620.0 13 7541.0 14 9669.0 15 12493.0 16 15788.0 17 20625.0 18 18259.0 19 13011.0 20 12186.0 21 12747.0 22 13643.0 23 15367.0 24 17826.0 25 21282.0 26 26076.0 27 32084.0 28 39734.0 29 48864.0 30 59591.0 31 73469.0 32 92578.0 33 117743.0 34 154376.0 35 209117.0 36 289655.0 37 351472.0 38 178314.0 39 7015.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.48657235267152 18.97894064415355 14.693532572709215 22.84095443046571 2 15.93738311063007 20.5523677641335 39.32358691528787 24.18666220994856 3 19.849136524654316 24.467672529276353 29.72062456927035 25.962566376798986 4 13.219981113857973 16.844953262103576 36.49741641821133 33.43764920582712 5 13.788948850553979 37.29720495642228 33.74854369550132 15.165302497522426 6 31.93779688549411 35.89644697626401 17.310155088685008 14.855601049556865 7 28.84940832200694 32.6348636429709 20.25891018356713 18.25681785145503 8 26.920167220176772 34.01222320073848 19.45626916506626 19.611340414018482 9 26.54291678713624 16.728408979381797 20.22047920805736 36.508195025424605 10-11 25.280532587509935 26.39510319970546 26.795663203864645 21.52870100891996 12-13 26.44083298231393 23.731847345285324 27.48076113032139 22.346558542079357 14-15 23.03459036083218 25.166978253304023 26.21505525799798 25.58337612786582 16-17 22.245969416961422 28.381835438572107 26.911917389825778 22.460277754640696 18-19 22.07599705987598 26.660457851900404 29.55570015085245 21.707844937371167 20-21 22.83904033918689 25.67817086507883 29.631425504322728 21.851363291411545 22-23 22.461897493926994 25.686969789085406 29.604064974660222 22.247067742327374 24-25 22.463117665377 25.890738421236076 29.712952013839928 21.933191899546998 26-27 22.219366318228694 25.993615860930387 29.687917775429394 22.099100045411525 28-29 21.920578126769744 26.272762455075892 29.79467470422171 22.011984713932662 30-31 22.15540732492279 25.97402459607565 29.55213476249426 22.318433316507296 32-33 22.036435382609184 26.070042006954118 29.582540640852745 22.310981969583953 34-35 22.77524310731736 26.03542761953771 29.202241945495157 21.987087327649775 36-37 22.624361293320845 25.809545712206855 29.317415766847226 22.248677227625073 38-39 22.3292041952425 26.033571065156348 29.478560366959044 22.15866437264211 40-41 22.452984386352043 25.98388687870543 29.38672472546871 22.176404009473814 42-43 22.387616680504312 26.093885530423805 28.90236106432249 22.616136724749396 44-45 22.52369423409577 26.190812825829706 28.77256851094713 22.512924429127388 46-47 22.818392285141638 26.56937505106139 28.1310312119029 22.481201451894073 48-49 22.969129570496296 26.649293904378013 27.91927925672023 22.462297268405464 50-51 22.78787904509734 26.80643746017835 27.97064882302979 22.435034671694517 52-53 23.093375052752734 26.913269602994415 27.63545482204392 22.357900522208933 54-55 22.622644342150565 26.765387274049683 27.80247885524697 22.809489528552778 56-57 22.859078666272985 27.07466686403734 27.505380605731656 22.56087386395802 58-59 23.110672195727258 26.665488916281312 27.488243053726375 22.73559583426506 60-61 22.79885862863015 26.73861645990783 28.070472811790175 22.392052099671844 62-63 22.701163221582398 26.801444365074335 28.058964005197932 22.438428408145334 64-65 22.55760580140409 26.855659355846367 27.89660373394563 22.690131108803914 66-67 22.551629523684223 27.007646814163422 27.565348589472077 22.875375072680278 68-69 22.61785743179886 26.83346972013958 27.790520475925852 22.75815237213571 70-71 22.648594562877275 26.782832350254175 27.662245197873197 22.90632788899535 72-73 22.745106673185 26.725764938344177 27.663779129250564 22.865349259220256 74-75 22.63763496367633 27.27315411922059 27.2920149464761 22.79719597062698 76-77 22.746325397911686 26.982210819717707 27.430309087803174 22.841154694567436 78-79 22.76410794705307 26.974507770701244 27.436662027085706 22.824722255159976 80-81 22.85794167274336 27.193426187183796 27.237486096989628 22.711146043083215 82-83 22.807889182414996 27.221435428427128 27.239977176937884 22.730698212219995 84-85 22.74114817838766 27.155321278908577 27.139193538717983 22.96433700398578 86-87 22.7389608556935 27.104591363063896 27.177006154992057 22.979441626250548 88-89 22.79973081449835 27.107706827597934 26.97444037506655 23.11812198283717 90-91 22.63421088722845 27.027259321274492 27.223854101259974 23.114675690237082 92-93 22.688725665735603 26.950812888439717 27.34098342391953 23.019478021905147 94-95 22.534465937780674 26.85393508780213 27.41044055179453 23.201158422622665 96-97 22.508399279993508 26.678622565826853 27.47621596587343 23.336762188306206 98-99 22.35668627614295 26.876671443039235 27.085401223253598 23.681241057564222 100-101 22.3670361198293 26.907983668514994 27.088917041743326 23.63606316991238 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 243.0 1 200.0 2 279.5 3 394.5 4 308.0 5 261.0 6 403.0 7 781.5 8 1255.0 9 1583.5 10 1609.0 11 1530.5 12 1624.5 13 1794.5 14 1999.0 15 2214.0 16 2389.5 17 2567.0 18 2820.0 19 3111.5 20 3384.0 21 3860.5 22 4479.5 23 5178.0 24 6175.5 25 7580.0 26 9555.5 27 12063.0 28 14354.5 29 17111.5 30 20961.0 31 25270.0 32 29782.0 33 33029.0 34 35879.0 35 38866.0 36 42249.0 37 46135.0 38 48746.5 39 50113.0 40 52033.5 41 55365.5 42 58032.5 43 60620.0 44 63771.0 45 66571.0 46 68265.0 47 71443.5 48 80645.5 49 80607.5 50 71817.5 51 67451.0 52 64875.5 53 64747.5 54 63974.5 55 59687.0 56 54243.5 57 49325.0 58 45778.5 59 41220.5 60 34761.5 61 28683.5 62 23548.0 63 18400.5 64 13425.0 65 10124.0 66 7771.5 67 6297.0 68 4825.0 69 3338.5 70 2472.5 71 1941.5 72 1620.0 73 1400.0 74 1154.0 75 916.5 76 647.0 77 392.5 78 240.0 79 183.0 80 126.5 81 73.5 82 55.5 83 38.0 84 17.0 85 10.0 86 9.0 87 5.5 88 2.5 89 2.0 90 3.0 91 3.0 92 1.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011565072631839176 2 0.010132701250831572 3 0.005464231564584565 4 3.1830475133502316E-4 5 0.004137961767355302 6 0.0 7 4.2440633511336425E-4 8 0.002440336426901844 9 5.305079188917053E-5 10-11 0.0 12-13 0.0 14-15 5.305079188917053E-4 16-17 0.004509317310579495 18-19 0.0016710999445088716 20-21 3.9788093916877897E-4 22-23 2.6525395944585264E-4 24-25 2.6525395944585264E-4 26-27 1.0610158377834106E-4 28-29 7.957618783375579E-5 30-31 5.570333148362906E-4 32-33 0.0031830475133502317 34-35 8.488126702267285E-4 36-37 0.001299744401284678 38-39 0.004721520478136177 40-41 2.6525395944585266E-5 42-43 0.0016445745485642864 44-45 0.004588893498413251 46-47 0.0 48-49 0.0022811840512343327 50-51 0.0010079650458942402 52-53 6.631348986146316E-4 54-55 1.5915237566751158E-4 56-57 0.011008039317002885 58-59 0.0019363539039547243 60-61 0.003209572909294817 62-63 2.9177935539043793E-4 64-65 0.0033421998890177432 66-67 9.549142540050695E-4 68-69 0.0015119475688413601 70-71 0.004880672853803688 72-73 0.0024668618228464298 74-75 0.006737450569924657 76-77 0.0010610158377834106 78-79 0.006233468046977538 80-81 0.0027851665741814528 82-83 0.0026790649904031116 84-85 0.0018567777161209685 86-87 6.366095026700463E-4 88-89 0.0021485570715114063 90-91 0.007665839427985141 92-93 0.0021750824674559915 94-95 4.7745712700253477E-4 96-97 1.5915237566751158E-4 98-99 6.896602945592169E-4 100-101 0.0020159300917884804 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1884986.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.84135626330682 #Duplication Level Percentage of deduplicated Percentage of total 1 80.1162561259354 47.141491691885015 2 11.90324478153532 14.008061337593352 3 3.4052063631903877 6.011008822996948 4 1.453110487526236 3.4201196754591483 5 0.7692083296928413 2.2630630684079818 6 0.46631226439678863 1.646306764759246 7 0.31809651237414416 1.3102061147505684 8 0.22712317521206465 1.069138853464524 9 0.16413988896461537 0.8692392315227901 >10 1.045645757777633 11.530801008031347 >50 0.08821554050247095 3.5313503906207377 >100 0.03918811092021373 4.249878162088367 >500 0.0029859115973615074 1.147448647911542 >1k 0.0010857860354041846 1.0974021682791715 >5k 1.8096433923403077E-4 0.7044840622292838 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6952 0.3688091052135135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6280 0.3331589730639909 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3716 0.19713674266015768 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3452 0.18313133360141667 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.10939073287546963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 5.305079188917053E-5 0.0 0.0 0.0 0.0 9 5.305079188917053E-5 0.0 0.0 0.0 0.0 10-11 5.305079188917053E-5 0.0 0.0 0.0 0.0 12-13 5.305079188917053E-5 0.0 0.0 0.0 0.0 14-15 1.8567777161209684E-4 0.0 0.0 0.0 2.6525395944585266E-5 16-17 2.1220316755668213E-4 0.0 0.0 0.0 5.305079188917053E-5 18-19 2.6525395944585264E-4 0.0 0.0 0.0 5.305079188917053E-5 20-21 3.7135554322419373E-4 0.0 0.0 0.0 5.305079188917053E-5 22-23 3.7135554322419373E-4 0.0 0.0 7.95761878337558E-5 5.305079188917053E-5 24-25 3.7135554322419373E-4 0.0 0.0 2.6525395944585264E-4 5.305079188917053E-5 26-27 3.7135554322419373E-4 0.0 0.0 4.5093173105794954E-4 5.305079188917053E-5 28-29 4.7745712700253477E-4 0.0 0.0 6.896602945592169E-4 5.305079188917053E-5 30-31 4.7745712700253477E-4 0.0 0.0 0.0014854221728967747 5.305079188917053E-5 32-33 4.7745712700253477E-4 0.0 0.0 0.002493387218791015 5.305079188917053E-5 34-35 5.570333148362906E-4 0.0 0.0 0.003633979244408181 5.305079188917053E-5 36-37 6.366095026700463E-4 0.0 0.0 0.005198977605138712 5.305079188917053E-5 38-39 6.366095026700463E-4 0.0 0.0 0.006339569630755878 5.305079188917053E-5 40-41 6.366095026700463E-4 0.0 0.0 0.008143296554987677 5.305079188917053E-5 42-43 6.896602945592169E-4 0.0 0.0 0.011458971048060835 5.305079188917053E-5 44-45 7.427110864483875E-4 0.0 0.0 0.01633964390186452 5.305079188917053E-5 46-47 7.957618783375579E-4 0.0 0.0 0.0302389513768272 7.95761878337558E-5 48-49 7.957618783375579E-4 0.0 0.0 0.05514629816879277 1.5915237566751158E-4 50-51 7.957618783375579E-4 0.0 0.0 0.07188382300982607 1.5915237566751158E-4 52-53 7.957618783375579E-4 0.0 0.0 0.09023939700347908 1.5915237566751158E-4 54-55 7.957618783375579E-4 0.0 0.0 0.11782580878584775 1.5915237566751158E-4 56-57 7.957618783375579E-4 0.0 0.0 0.13819731287128922 1.5915237566751158E-4 58-59 7.957618783375579E-4 0.0 0.0 0.1602399169012396 1.5915237566751158E-4 60-61 7.957618783375579E-4 0.0 0.0 0.19833038547766402 1.5915237566751158E-4 62-63 7.957618783375579E-4 0.0 0.0 0.2239008671682442 1.5915237566751158E-4 64-65 7.957618783375579E-4 0.0 0.0 0.2537154122099581 1.5915237566751158E-4 66-67 9.01863462115899E-4 2.6525395944585266E-5 0.0 0.3175620402485748 1.8567777161209684E-4 68-69 9.01863462115899E-4 5.305079188917053E-5 0.0 0.3935042488379224 2.1220316755668213E-4 70-71 9.01863462115899E-4 5.305079188917053E-5 0.0 0.454804438865859 2.1220316755668213E-4 72-73 9.283888580604843E-4 5.305079188917053E-5 0.0 0.5263434317284055 2.1220316755668213E-4 74-75 9.549142540050695E-4 5.305079188917053E-5 0.0 0.5848054043902713 2.1220316755668213E-4 76-77 9.549142540050695E-4 5.305079188917053E-5 0.0 0.6308269663541268 2.1220316755668213E-4 78-79 9.549142540050695E-4 5.305079188917053E-5 0.0 0.6947001197886882 2.1220316755668213E-4 80-81 0.0011140666296725811 5.305079188917053E-5 0.0 0.8042500050398252 2.1220316755668213E-4 82-83 0.0011140666296725811 5.305079188917053E-5 0.0 0.907805150807486 2.1220316755668213E-4 84-85 0.001193642817506337 5.305079188917053E-5 0.0 1.0241720628163817 2.1220316755668213E-4 86-87 0.001299744401284678 5.305079188917053E-5 0.0 1.2402214127850288 2.1220316755668213E-4 88-89 0.0013262697972292632 5.305079188917053E-5 0.0 1.5137247703696475 2.1220316755668213E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGG 60 0.0039536143 31.663351 6 TATACCG 90 2.4155057E-5 31.66335 5 TACCTGG 1775 0.0 29.43744 2 GTACCTG 1910 0.0 28.358347 1 TATCACG 110 9.4779825E-5 25.909815 2 GGTATCA 10150 0.0 24.57552 1 GTATCAA 13415 0.0 24.04853 1 GTACATG 8045 0.0 22.619457 1 GAGTACT 8245 0.0 22.382076 12-13 TACATGG 8255 0.0 21.46335 2 ACATGGG 8200 0.0 21.025238 3 GTACTTT 8975 0.0 20.509472 14-15 ATCAACG 15730 0.0 20.38089 3 TATCAAC 15950 0.0 20.191786 2 TCAACGC 15980 0.0 20.06204 4 CAACGCA 16175 0.0 19.849545 5 CCGTACG 60 0.0047625145 19.793795 78-79 AACGCAG 16475 0.0 19.488096 6 AGTACTT 8710 0.0 19.087536 12-13 GTACACG 385 1.2732926E-11 18.51143 1 >>END_MODULE