FastQCFastQC Report
Thu 26 May 2016
SRR936442_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936442_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956185
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63300.32358902660024486No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52950.2706799203551811No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30650.1566825223585704No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29570.15116157214169415No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT20100.10275101792519624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18200.029.768412
GTACCTG18800.029.3246921
GTACATG88350.025.6593191
TACATGG88650.025.4646112
GTATCAA127350.024.7427461
ACATGGG89050.024.1754933
GAGTACT65850.021.57627712-13
GGTATCA95900.021.5082171
CATGGGA45550.021.0763954
ATCAACG148050.021.0574863
TATCAAC148900.021.0335662
TCAACGC148900.020.9702684
ATAACGC1604.9723676E-520.7916583
CAACGCA149350.020.781395
CATGGGG43250.020.7686864
TATCACG1150.00371285920.6630462
AACGCAG153300.020.3074176
GTACTTT71250.019.83844614-15
TAGACGC1200.004749485319.8025934
AGTACTT68450.019.71541812-13
Produced by FastQC (version 0.11.5)