FastQCFastQC Report
Thu 26 May 2016
SRR936442_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936442_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1956185
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72860.3724596600014825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64420.3293144564547832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.19231309922118817No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33820.17288753364329038No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20550.10505141384889466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20600.029.9771822
GTACCTG22950.028.2319891
GTACATG86200.026.8924431
TACATGG86300.026.0354462
GTATCAA136550.025.2361531
ACATGGG84400.024.874893
CATGGGG40100.022.1502484
GGTATCA100250.021.7351111
TAACGCA1553.909078E-521.4510064
ATCAACG160850.021.4090943
GAGTACT86450.021.36810512-13
TCAACGC161600.021.3097324
TATCAAC161850.021.24912
CAACGCA163500.021.0594045
AACGCAG167250.020.5588256
AGTACTT91150.020.16209812-13
GTACTTT93500.019.75692714-15
CATGGGA45650.019.1451344
ACGCAGA182950.018.7685937
CGCAGAG183450.018.691558
Produced by FastQC (version 0.11.5)