##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936442_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1956185 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.49422830662744 31.0 31.0 34.0 30.0 34.0 2 31.706354460339895 31.0 31.0 34.0 30.0 34.0 3 31.989708539836467 33.0 31.0 34.0 30.0 34.0 4 35.792758353632195 37.0 35.0 37.0 35.0 37.0 5 35.603602931215605 37.0 35.0 37.0 33.0 37.0 6 35.70435464948356 37.0 35.0 37.0 33.0 37.0 7 35.642681034769204 37.0 35.0 37.0 33.0 37.0 8 35.618530967163125 37.0 35.0 37.0 33.0 37.0 9 37.250398607493665 39.0 37.0 39.0 33.0 39.0 10-11 37.13803474620243 39.0 37.0 39.0 33.0 39.0 12-13 37.00885473510941 39.0 37.0 39.0 33.0 39.0 14-15 38.138041136191106 40.0 37.0 41.0 33.0 41.0 16-17 38.05188619685766 40.0 37.0 41.0 33.0 41.0 18-19 38.11326715009061 40.0 37.5 41.0 33.0 41.0 20-21 38.18470211150786 40.0 37.5 41.0 33.0 41.0 22-23 38.10162970271217 40.0 37.0 41.0 33.0 41.0 24-25 37.99631144293612 40.0 37.0 41.0 33.0 41.0 26-27 37.79033424752771 40.0 37.0 41.0 32.5 41.0 28-29 37.70504042306837 40.0 37.0 41.0 32.5 41.0 30-31 37.69608523733696 40.0 37.0 41.0 33.0 41.0 32-33 37.52925387936212 40.0 36.5 41.0 32.0 41.0 34-35 37.356190748829995 39.0 36.0 41.0 32.0 41.0 36-37 37.11507014929569 39.0 36.0 41.0 31.0 41.0 38-39 36.96830079977099 39.0 36.0 41.0 31.0 41.0 40-41 36.73778783703995 39.0 35.0 41.0 30.5 41.0 42-43 36.499036645307065 39.0 35.0 40.5 30.0 41.0 44-45 36.231871218724194 39.0 35.0 40.0 30.0 41.0 46-47 35.83843603749134 39.0 35.0 40.0 28.5 41.0 48-49 35.394072646503275 38.0 35.0 40.0 27.0 41.0 50-51 35.18286869595667 38.0 34.0 40.0 27.0 41.0 52-53 34.9017667040694 38.0 34.0 40.0 26.0 41.0 54-55 34.63003908117075 38.0 34.0 40.0 26.0 41.0 56-57 34.354623412407314 38.0 33.5 40.0 25.0 41.0 58-59 33.98883029979271 37.0 33.0 40.0 23.5 41.0 60-61 33.605877767184595 37.0 33.0 39.5 23.0 41.0 62-63 33.314503740699365 36.0 32.0 39.0 23.0 40.5 64-65 32.87264982606451 36.0 32.0 39.0 22.0 40.0 66-67 32.46563029570312 35.0 31.0 38.5 21.0 40.0 68-69 32.01934275132464 35.0 31.0 38.0 20.0 40.0 70-71 31.540069574196714 35.0 30.5 37.5 18.5 39.5 72-73 31.12338761415715 34.0 30.0 37.0 18.0 39.0 74-75 30.756583094134758 34.0 30.0 36.0 16.0 39.0 76-77 29.331343405659485 32.5 28.5 35.0 13.0 37.0 78-79 30.13262012539714 34.0 29.5 35.0 12.5 37.0 80-81 29.971320964019252 34.0 30.0 35.0 9.0 37.0 82-83 29.636502938116795 34.0 29.0 35.0 7.0 36.5 84-85 29.197453717312015 34.0 29.0 35.0 4.5 36.0 86-87 28.804403213397507 34.0 29.0 35.0 2.0 36.0 88-89 28.39387506805338 33.0 28.5 35.0 2.0 35.0 90-91 27.93736814258365 33.0 27.0 35.0 2.0 35.0 92-93 27.470725672674106 33.0 26.5 35.0 2.0 35.0 94-95 26.935759399034346 32.5 25.5 34.0 2.0 35.0 96-97 26.24420082967613 32.0 25.0 34.0 2.0 35.0 98-99 25.361491627836834 32.0 22.0 34.0 2.0 35.0 100-101 22.49651975656699 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36202906955188396 1101 2 0.3558798143691888 1101 3 0.24562678495320966 1101 4 0.13123703911685425 1101 5 0.1516037568681412 1101 6 0.0332754691555337 1101 7 0.14919967941497703 1101 8 0.04680396157151279 1101 9 0.15112248930399375 1101 10-11 0.11842965875399614 1101 12-13 0.07469658689179681 1101 14-15 0.1764681792635585 1101 16-17 0.11053117281131364 1101 18-19 0.21338971749280233 1101 20-21 0.10144818086432394 1101 22-23 0.009125391246328718 1101 24-25 0.12931843283189437 1101 26-27 0.071852077118983 1101 28-29 0.028285133158952647 1101 30-31 0.02386241420610702 1101 32-33 0.09434269371142534 1101 34-35 0.20602368657411319 1101 36-37 0.2332417249603651 1101 38-39 0.2112211569260296 1101 40-41 0.28491373006232124 1101 42-43 0.38531037235787835 1101 44-45 0.3476905652478308 1101 46-47 0.3775409400492933 1101 48-49 0.3048382932256928 1101 50-51 0.07405324621365139 1101 52-53 0.09941559646486553 1101 54-55 0.06402302246826252 1101 56-57 0.018750469813276993 1101 58-59 0.04033664047805985 1101 60-61 0.1929929250800413 1101 62-63 -0.0323654148985284 1101 64-65 -0.019799977215804176 1101 66-67 -0.2485048009747146 1101 68-69 -0.39743375401693015 1101 70-71 -0.22638602159819143 1101 72-73 -0.107688751645302 1101 74-75 -0.09736747332803475 1101 76-77 0.34547960657229737 1101 78-79 0.1653289061894192 1101 80-81 0.1360558268940153 1101 82-83 0.062380459553402545 1101 84-85 0.14654493032834637 1101 86-87 0.03451566094049596 1101 88-89 -0.04678780849219066 1101 90-91 -0.18471550635004164 1101 92-93 -0.18463511368159047 1101 94-95 -0.27144361649930815 1101 96-97 -0.263750885532712 1101 98-99 -0.18103800609484466 1101 100-101 -0.139676614207616 1102 1 0.5379681302318282 1102 2 0.5499294826031189 1102 3 0.31882187849379306 1102 4 0.18963824262362294 1102 5 0.13953402704409257 1102 6 0.2636144977671151 1102 7 0.2155651549934987 1102 8 0.06392799539420224 1102 9 0.12876164477424368 1102 10-11 0.23518714648077577 1102 12-13 0.2866803687892059 1102 14-15 0.34070535309766825 1102 16-17 0.3407012530293798 1102 18-19 0.28466990537567227 1102 20-21 0.22908651104584266 1102 22-23 0.16167517973661205 1102 24-25 0.18967722121371366 1102 26-27 -3.526699418046064E-4 1102 28-29 0.052145763113635724 1102 30-31 0.0478297422041436 1102 32-33 0.416102256449868 1102 34-35 0.3338521868594242 1102 36-37 0.36317365042971517 1102 38-39 0.25972078200914694 1102 40-41 0.4401736488539143 1102 42-43 0.46182100443472507 1102 44-45 0.13790122609189126 1102 46-47 0.17763407977775358 1102 48-49 0.36733740898776546 1102 50-51 0.29510801237398 1102 52-53 0.179992426129985 1102 54-55 0.17914349104362515 1102 56-57 0.24599336879321498 1102 58-59 0.2987203950230466 1102 60-61 0.18997201462151025 1102 62-63 0.16966102263653937 1102 64-65 0.23454505006588278 1102 66-67 0.43341866410614216 1102 68-69 0.43830219131594816 1102 70-71 0.2141995561275003 1102 72-73 0.11465688399207963 1102 74-75 -0.2591915029063827 1102 76-77 0.07957240203745641 1102 78-79 0.22133995562657205 1102 80-81 0.21343996814571753 1102 82-83 0.30848438934255284 1102 84-85 0.45745949172275147 1102 86-87 0.2647840684161942 1102 88-89 0.2996931603496229 1102 90-91 0.2936032154427686 1102 92-93 0.37053484863808706 1102 94-95 0.4292131954338956 1102 96-97 0.3094977338771372 1102 98-99 -0.03383443801795849 1102 100-101 0.026382443454131987 1103 1 0.366694982145475 1103 2 0.36724991502997284 1103 3 0.26438589049951844 1103 4 0.12021823171418333 1103 5 0.09759162107575037 1103 6 0.08540037190630301 1103 7 0.057247416113611393 1103 8 0.19819290266838152 1103 9 0.034386281608121294 1103 10-11 0.11608189437563254 1103 12-13 0.074223070006596 1103 14-15 0.15378903280138445 1103 16-17 0.08805364407508876 1103 18-19 0.17596855628860908 1103 20-21 0.1476413078641272 1103 22-23 0.07384092230400086 1103 24-25 0.056083698540135174 1103 26-27 0.05017691858707707 1103 28-29 0.1880830142363905 1103 30-31 0.13372915286282705 1103 32-33 0.02592969859467331 1103 34-35 0.07054773774628131 1103 36-37 0.20907675837536743 1103 38-39 0.05453559286912224 1103 40-41 0.05736340821436414 1103 42-43 0.15620508803701938 1103 44-45 0.35582715043587854 1103 46-47 0.31361126248958016 1103 48-49 0.30401302744910197 1103 50-51 0.09541447341631937 1103 52-53 0.16388531336851742 1103 54-55 0.32381930882922205 1103 56-57 0.15870347124054263 1103 58-59 0.12504000612718613 1103 60-61 0.13628093429294097 1103 62-63 0.06207620082263787 1103 64-65 0.1503741544326047 1103 66-67 -0.012560347227761781 1103 68-69 -0.057376298660450686 1103 70-71 -0.27385366878767314 1103 72-73 -0.22744660533917838 1103 74-75 -0.15451823896280814 1103 76-77 -0.08882422564530046 1103 78-79 -0.11157971313923198 1103 80-81 -0.10955397560034896 1103 82-83 -0.10727664223165334 1103 84-85 -0.13195122925265323 1103 86-87 -0.16466704686530065 1103 88-89 -0.2173564046518841 1103 90-91 -0.27782405300517965 1103 92-93 -0.08445958096085704 1103 94-95 -0.06687384252758477 1103 96-97 -0.45956976674797545 1103 98-99 -0.6132216627569456 1103 100-101 -0.26132910498388995 1104 1 0.2420591316995946 1104 2 0.28163209070970296 1104 3 0.032426033399516996 1104 4 -0.009557873442766152 1104 5 -0.12568602489223224 1104 6 0.05159372323849709 1104 7 0.008930854440265534 1104 8 -0.029596674358536745 1104 9 -0.030053981284240194 1104 10-11 -0.08005740585071663 1104 12-13 -0.0995441993489905 1104 14-15 0.03718678021746058 1104 16-17 -0.033879869246788985 1104 18-19 -0.010751343354144183 1104 20-21 -0.1120659107674129 1104 22-23 -0.15711481687623774 1104 24-25 -0.09346289394943597 1104 26-27 -0.045801919123242385 1104 28-29 -0.1800097071358877 1104 30-31 -0.2953374701702458 1104 32-33 -0.20582322621920213 1104 34-35 -0.16858362662144089 1104 36-37 -0.19737021318098869 1104 38-39 -0.20845625310432325 1104 40-41 -0.1654675389071798 1104 42-43 -0.15747153254167756 1104 44-45 -0.04998135265937975 1104 46-47 0.1159800208413202 1104 48-49 0.1314681530320172 1104 50-51 0.003441308072304139 1104 52-53 -0.13120154474051304 1104 54-55 0.052008569512629776 1104 56-57 0.0296861541607214 1104 58-59 0.02756311829276825 1104 60-61 -0.1401893266904537 1104 62-63 -0.3688400499613991 1104 64-65 -0.2506431626472221 1104 66-67 -0.11296035813614935 1104 68-69 0.011254206671033273 1104 70-71 -0.16241261503655124 1104 72-73 -0.2437257512117128 1104 74-75 -0.1791174371956501 1104 76-77 -0.04250651859029375 1104 78-79 0.10118900157892341 1104 80-81 0.12420151268675994 1104 82-83 0.13924243382713897 1104 84-85 0.25200121013756416 1104 86-87 0.13469950253610108 1104 88-89 0.29336076789168075 1104 90-91 0.18428290382484036 1104 92-93 0.17406527654821247 1104 94-95 0.15076710759376866 1104 96-97 0.022458104205689722 1104 98-99 0.12349501890876624 1104 100-101 0.11513766531254532 1105 1 0.387154481424286 1105 2 0.4163396085639235 1105 3 0.16093238459615833 1105 4 0.16103690385647695 1105 5 0.06668141772892966 1105 6 0.01877095706066001 1105 7 0.1199454104016695 1105 8 0.005972861704265142 1105 9 0.03286484333550277 1105 10-11 0.09441536305295273 1105 12-13 0.07232975801893815 1105 14-15 0.23706608022290965 1105 16-17 0.09586771838881702 1105 18-19 0.11559407451643722 1105 20-21 0.2049481000268969 1105 22-23 0.10920759494299404 1105 24-25 -0.02155927949159775 1105 26-27 -0.005821763205453578 1105 28-29 0.11097453753510678 1105 30-31 0.029078819422508673 1105 32-33 0.07942215348968062 1105 34-35 0.08053462012118473 1105 36-37 0.1201807872006242 1105 38-39 0.14349032052049893 1105 40-41 -0.04704438020903723 1105 42-43 0.05302162561130075 1105 44-45 -0.053632663786238766 1105 46-47 0.18146440180408518 1105 48-49 0.2619842082029322 1105 50-51 0.2027902695289825 1105 52-53 0.2676497881930331 1105 54-55 0.3115553175909227 1105 56-57 0.3055866066494062 1105 58-59 0.21765525153161036 1105 60-61 0.2886138134720042 1105 62-63 0.08621888260142896 1105 64-65 0.060463962686689854 1105 66-67 0.0020560513933673974 1105 68-69 -0.13359472752729928 1105 70-71 0.019345875940935287 1105 72-73 0.04298743638028313 1105 74-75 -0.08690972853294454 1105 76-77 0.044061763588192804 1105 78-79 -0.04971881066552797 1105 80-81 -0.13265450868379247 1105 82-83 0.12301684368376087 1105 84-85 0.13053285708735984 1105 86-87 -0.03474573542933612 1105 88-89 -0.0017237875245541545 1105 90-91 -0.009360447862452048 1105 92-93 0.2920990769080234 1105 94-95 0.6000054001304314 1105 96-97 0.5349664180038687 1105 98-99 0.5699264641467323 1105 100-101 0.8384233873863636 1106 1 0.44371656465401443 1106 2 0.43507873015347087 1106 3 0.2054349728101741 1106 4 0.08777575419215822 1106 5 -0.053625325190211015 1106 6 0.08148711248912832 1106 7 0.09966918354301413 1106 8 0.07167846083625307 1106 9 0.14418065065483887 1106 10-11 0.03817821935621879 1106 12-13 0.12963778939397486 1106 14-15 0.11284310954092547 1106 16-17 0.07066653843448023 1106 18-19 0.00861550357225127 1106 20-21 0.007537444270667493 1106 22-23 0.020589520393379246 1106 24-25 -0.0049675843809495746 1106 26-27 0.04929928383448612 1106 28-29 0.09849685946824849 1106 30-31 -0.06496433254746847 1106 32-33 0.08506762468025642 1106 34-35 -0.08484264880441827 1106 36-37 0.04488240794250942 1106 38-39 0.099513286681578 1106 40-41 0.0842396678028976 1106 42-43 0.21987666302172926 1106 44-45 0.1620452448464107 1106 46-47 0.17891292007981718 1106 48-49 0.04695617863626467 1106 50-51 0.045965974223939554 1106 52-53 0.3606050991177838 1106 54-55 0.31286355240634833 1106 56-57 -0.04971358096785394 1106 58-59 -0.04854934170059977 1106 60-61 0.041131388462403606 1106 62-63 -0.3072941940955971 1106 64-65 0.18257623918480448 1106 66-67 0.11710442500036322 1106 68-69 0.2599240002920453 1106 70-71 0.2514205159644405 1106 72-73 0.3065981644708806 1106 74-75 0.09971293472798237 1106 76-77 0.19884035261597433 1106 78-79 0.18302831291321198 1106 80-81 0.14362321007115497 1106 82-83 0.22910536286367034 1106 84-85 0.2676658166931354 1106 86-87 0.3464704991148473 1106 88-89 0.15160246306881575 1106 90-91 0.10838068552664026 1106 92-93 0.5457751319725581 1106 94-95 0.41359747951739934 1106 96-97 0.41063325043678844 1106 98-99 0.27334426292156877 1106 100-101 0.47195093095651686 1107 1 0.5928216783200249 1107 2 0.589794100786257 1107 3 0.42440090744256764 1107 4 0.16464352680587524 1107 5 0.11862307672394223 1107 6 0.20068042087639526 1107 7 0.23771708750968656 1107 8 0.17433004160037058 1107 9 0.25501570398697737 1107 10-11 0.31410107088814243 1107 12-13 0.3552522411404482 1107 14-15 0.4443559224015843 1107 16-17 0.5250751788458601 1107 18-19 0.3806066361001541 1107 20-21 0.31569879511494037 1107 22-23 0.3917834360845731 1107 24-25 0.3376316555500125 1107 26-27 0.4472351809225543 1107 28-29 0.48431954759158913 1107 30-31 0.3806232705220225 1107 32-33 0.5140934131840567 1107 34-35 0.46539896841880335 1107 36-37 0.560391875414183 1107 38-39 0.6551003784470808 1107 40-41 0.7139321862776669 1107 42-43 0.49074256154980134 1107 44-45 0.4178550668917609 1107 46-47 0.568105258032972 1107 48-49 0.6856319952096044 1107 50-51 0.5404136302603533 1107 52-53 0.2594796042485612 1107 54-55 0.46185257919659506 1107 56-57 0.4714974477549063 1107 58-59 0.43896205829146595 1107 60-61 0.4715796977157467 1107 62-63 0.28740060959496816 1107 64-65 0.34970873161437765 1107 66-67 0.5018192008509601 1107 68-69 0.5616969207978357 1107 70-71 0.3767927765750443 1107 72-73 0.44847015273384727 1107 74-75 0.6331344766956022 1107 76-77 0.5520548895102095 1107 78-79 0.7616115625944317 1107 80-81 0.7978382332259208 1107 82-83 0.7387022257528137 1107 84-85 0.7533678266395611 1107 86-87 0.9332760909022575 1107 88-89 1.0346834139690202 1107 90-91 0.9654122632438025 1107 92-93 1.2490907296660545 1107 94-95 1.3894550615295138 1107 96-97 1.4547449588808163 1107 98-99 1.4674884756908142 1107 100-101 1.4099999305745996 1108 1 0.3402679761732941 1108 2 0.31552473627250777 1108 3 0.15312401676376908 1108 4 0.14740101591155508 1108 5 -0.014053447547965447 1108 6 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-1.3106404874917885 2316 64-65 -1.241780377037113 2316 66-67 -1.3928927839990592 2316 68-69 -1.4892251321719456 2316 70-71 -1.2085853180020365 2316 72-73 -1.151962260691537 2316 74-75 -1.2940924007347832 2316 76-77 -1.346944170510568 2316 78-79 -1.2278389517511705 2316 80-81 -1.1880353614017167 2316 82-83 -1.1819712268437392 2316 84-85 -1.3986792711458556 2316 86-87 -1.2095079597876683 2316 88-89 -1.403088065360631 2316 90-91 -1.588064568221828 2316 92-93 -1.8750597842198964 2316 94-95 -1.9610962856974297 2316 96-97 -2.0319913367277103 2316 98-99 -2.192838598009075 2316 100-101 -3.0819691650228904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 34.0 5 61.0 6 138.0 7 340.0 8 891.0 9 1607.0 10 2618.0 11 4282.0 12 5863.0 13 8253.0 14 10159.0 15 13061.0 16 16450.0 17 20392.0 18 18119.0 19 12585.0 20 11666.0 21 11992.0 22 12909.0 23 14559.0 24 17167.0 25 20706.0 26 25477.0 27 31681.0 28 39115.0 29 48711.0 30 60474.0 31 73526.0 32 92260.0 33 116784.0 34 151994.0 35 204234.0 36 287020.0 37 373759.0 38 232857.0 39 14431.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40094652964956 18.986483903727127 14.754890971396456 22.857678595226854 2 15.812580204813056 20.564999769930417 39.42850715516404 24.193912870092486 3 19.862665648314863 24.496012989097707 29.74328537059928 25.89803599198815 4 13.283699680612454 16.85969494833918 36.48502937987077 33.37157599117761 5 13.7663360060526 37.27137259512776 33.77303271418603 15.18925868463361 6 31.93864588471949 35.89588919248435 17.369829540662053 14.795635382134103 7 28.96285371782321 32.585210499007 20.20780243177409 18.244133351395703 8 26.887385395553082 34.058793007818785 19.4469848199429 19.60683677668523 9 26.546958840465905 16.686450971731446 20.206779217379925 36.55981097042272 10-11 25.275256137222684 26.37336684084986 26.810596990662404 21.540780031265047 12-13 26.443270615924284 23.721616904766528 27.459229253719748 22.375883225589437 14-15 23.051717016395184 25.13155715413274 26.232067126609763 25.584658702862306 16-17 22.265353225794087 28.32206565329966 26.91453006745272 22.498051053453533 18-19 22.097194283771728 26.63584988127401 29.5665542880658 21.700401546888457 20-21 22.83280978026107 25.67732601977829 29.635821765323833 21.854042434636806 22-23 22.456120799564044 25.70129528884563 29.590561332729433 22.252022578860895 24-25 22.52125949232818 25.84543384189123 29.71848265884873 21.91482400693186 26-27 22.24143479308828 26.025714854605997 29.67930683429912 22.05354351800661 28-29 21.997944979641495 26.28013199160611 29.694584101196973 22.02733892755542 30-31 22.194577711208296 25.894457835020717 29.60765469523588 22.303309758535107 32-33 22.069887649525004 26.00578392534984 29.55668159935709 22.367646825768066 34-35 22.718449827980187 25.993599738264056 29.243726261010032 22.04422417274572 36-37 22.699782145768328 25.81637825199776 29.282543110113902 22.201296492120008 38-39 22.395669323540115 25.964198664338323 29.464355091341098 22.175776920780457 40-41 22.534568290009652 25.97511512539057 29.349740916749823 22.14057566784996 42-43 22.33219494987193 26.116519000608392 28.949278881793873 22.60200716772581 44-45 22.525026301771547 26.17596268573549 28.783748828050314 22.51526218444264 46-47 22.86938847287941 26.529968018845164 28.189271590346763 22.41137191792867 48-49 22.996839708577316 26.578861195900494 27.921556878727174 22.50274221679502 50-51 22.795538858623 26.795347669770848 28.008246680649695 22.40086679095646 52-53 23.09511808350859 26.863167494650224 27.652035265045054 22.389679156796127 54-55 22.65631450555973 26.70880060654877 27.844545802893634 22.790339084997864 56-57 22.874965460672197 27.04570591484551 27.514316088080204 22.565012536402087 58-59 23.191500075275087 26.62146242239573 27.495215750666418 22.69182175166276 60-61 22.839635557200566 26.700644451510726 28.07563793337907 22.384082057909637 62-63 22.765420889931086 26.67152830922705 28.077187138476038 22.485863662365826 64-65 22.588323475373677 26.821055344069077 27.85924076028784 22.7313804202694 66-67 22.560451301556142 27.015392716193283 27.55222072265505 22.871935259595528 68-69 22.658358301200142 26.836448160983057 27.76087693316723 22.74431660464957 70-71 22.762182396311125 26.807342474577005 27.532183712670665 22.898291416441204 72-73 22.75595681286709 26.7301473864045 27.63793496983541 22.875960830892996 74-75 22.65775544177965 27.247762161493462 27.30629447273506 22.78818792399183 76-77 22.79368049218752 26.98399674874052 27.40032154751772 22.82200121155424 78-79 22.787234700656885 26.95587289098094 27.470705778628506 22.78618662973367 80-81 22.857419675126707 27.193094128997323 27.200533155819233 22.74895304005673 82-83 22.819487290652887 27.206612398400377 27.28140935253343 22.692490958413302 84-85 22.76199274349105 27.116281542731553 27.174997200973195 22.946728512804206 86-87 22.810701210580966 27.06003007152852 27.202897950565514 22.926370767325 88-89 22.823788746733236 27.091611566508035 27.02621235262806 23.058387334130668 90-91 22.68369242977857 27.069904926109885 27.19412887793993 23.05227376617161 92-93 22.71208968729072 26.925894495764595 27.412646398756728 22.949369418187953 94-95 22.52095495977716 26.866800559599863 27.3960840790362 23.216160401586777 96-97 22.553736895692385 26.730627506245565 27.425953680198017 23.289681917864037 98-99 22.362628134880676 26.84252085447027 27.133421319220822 23.661429691428225 100-101 22.34304598585562 26.905000125257484 27.14863870681109 23.603315182075807 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 275.0 1 206.0 2 302.0 3 431.0 4 333.5 5 302.0 6 459.0 7 842.0 8 1365.5 9 1694.5 10 1688.0 11 1618.0 12 1713.0 13 1917.5 14 2132.5 15 2368.0 16 2520.0 17 2678.0 18 2968.0 19 3213.5 20 3522.5 21 4039.0 22 4755.0 23 5503.0 24 6420.0 25 7848.5 26 9901.5 27 12504.5 28 14900.5 29 17734.5 30 21668.0 31 26010.0 32 30945.5 33 34368.5 34 36847.0 35 39850.0 36 43772.5 37 48068.0 38 50440.0 39 51948.5 40 53650.0 41 57115.0 42 60313.0 43 62904.5 44 66123.5 45 68933.5 46 70675.5 47 74036.5 48 83422.5 49 82968.5 50 73835.0 51 70114.0 52 67910.0 53 67510.5 54 66568.0 55 62134.0 56 56484.5 57 51198.5 58 47882.5 59 43443.0 60 36217.0 61 29693.0 62 24441.0 63 19002.5 64 14024.5 65 10692.5 66 8144.5 67 6552.0 68 5022.5 69 3547.0 70 2599.5 71 2037.5 72 1722.0 73 1416.5 74 1121.5 75 907.0 76 643.5 77 392.5 78 261.5 79 199.5 80 144.0 81 86.0 82 60.5 83 38.0 84 15.5 85 14.0 86 10.0 87 5.5 88 5.5 89 4.0 90 2.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0362516837620164 2 0.01303557690095773 3 0.004242952481488203 4 0.012984456991542211 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004549671937981326 10-11 3.578393659086436E-4 12-13 1.5335972824656154E-4 14-15 5.111990941552052E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.712782788948898E-4 24-25 0.0 26-27 2.555995470776026E-5 28-29 0.0 30-31 0.0 32-33 0.003782873296748518 34-35 7.667986412328077E-4 36-37 0.0030927545196389908 38-39 5.111990941552052E-5 40-41 0.002913834836684669 42-43 0.009968382336026501 44-45 0.0021981561048673823 46-47 0.001686957010712177 48-49 0.009942822381318741 50-51 2.0447963766208208E-4 52-53 0.001380237554219054 54-55 0.010249541837811864 56-57 0.004166272617364923 58-59 0.0013546775995112936 60-61 0.0052653506697986135 62-63 0.0028627149272691488 64-65 0.016383930967674324 66-67 0.003322794112008833 68-69 2.0447963766208208E-4 70-71 0.0016613970560044166 72-73 2.555995470776026E-5 74-75 2.555995470776026E-5 76-77 0.0010223981883104103 78-79 0.010760740931967069 80-81 0.014518054274007826 82-83 0.011399739799661076 84-85 0.007565746593497036 86-87 0.009303823513624734 88-89 0.02576443434542234 90-91 0.02264611987107559 92-93 0.0028115950178536284 94-95 0.007386826910542714 96-97 0.01004506220014978 98-99 0.009687222834241137 100-101 0.01093966061492139 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1956185.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.86198682962282 #Duplication Level Percentage of deduplicated Percentage of total 1 80.16128868111704 47.18452718593504 2 11.832892337238286 13.930151058217296 3 3.3706221023116587 5.952045413817133 4 1.4590744682107712 3.435360885250453 5 0.7835295310146666 2.3060052467602925 6 0.48795817148321446 1.7233312477951082 7 0.3237264796263709 1.3338628646117323 8 0.242665767507473 1.1427031368820153 9 0.17298738073010825 0.9164142833603924 >10 1.0365952949049315 11.44876800651064 >50 0.08395569557748976 3.368927675325347 >100 0.04042719220102894 4.311181969713599 >500 0.0030549271977668093 1.1820792844929118 >1k 0.001047403610662905 1.0593399151147134 >5k 1.7456726844381748E-4 0.7053018262134335 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7286 0.3724596600014825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6442 0.3293144564547832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3762 0.19231309922118817 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3382 0.17288753364329038 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2055 0.10505141384889466 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 1.0223981883104103E-4 0.0 0.0 0.0 0.0 8 1.0223981883104103E-4 0.0 0.0 0.0 0.0 9 1.0223981883104103E-4 0.0 0.0 1.0223981883104103E-4 0.0 10-11 1.0223981883104103E-4 0.0 0.0 1.0223981883104103E-4 0.0 12-13 1.0223981883104103E-4 0.0 0.0 1.0223981883104103E-4 0.0 14-15 1.0223981883104103E-4 0.0 0.0 1.0223981883104103E-4 0.0 16-17 1.0223981883104103E-4 0.0 0.0 1.0223981883104103E-4 0.0 18-19 2.0447963766208205E-4 0.0 0.0 1.0223981883104103E-4 5.111990941552051E-5 20-21 3.067194564931231E-4 0.0 0.0 1.0223981883104103E-4 5.111990941552051E-5 22-23 3.067194564931231E-4 0.0 0.0 1.5335972824656154E-4 5.111990941552051E-5 24-25 3.067194564931231E-4 0.0 0.0 1.5335972824656154E-4 5.111990941552051E-5 26-27 3.067194564931231E-4 0.0 0.0 2.555995470776026E-4 7.667986412328077E-5 28-29 4.3451923003192435E-4 0.0 0.0 4.3451923003192435E-4 1.0223981883104103E-4 30-31 4.6007918473968466E-4 5.111990941552051E-5 0.0 8.179185506483283E-4 1.0223981883104103E-4 32-33 5.111990941552052E-4 5.111990941552051E-5 0.0 0.001584717191881136 1.0223981883104103E-4 34-35 5.878789582784859E-4 5.111990941552051E-5 0.0 0.0024793156066527447 1.0223981883104103E-4 36-37 6.134389129862462E-4 5.111990941552051E-5 0.0 0.004012912889118361 1.0223981883104103E-4 38-39 7.156787318172872E-4 5.111990941552051E-5 0.0 0.005393150443337415 1.0223981883104103E-4 40-41 7.156787318172872E-4 5.111990941552051E-5 0.0 0.007437946819958235 1.0223981883104103E-4 42-43 7.412386865250475E-4 5.111990941552051E-5 0.0 0.010147302018980823 1.0223981883104103E-4 44-45 7.923585959405679E-4 5.111990941552051E-5 0.0 0.015233733005825114 1.0223981883104103E-4 46-47 8.690384600638488E-4 5.111990941552051E-5 0.0 0.026837952443148273 1.0223981883104103E-4 48-49 8.690384600638488E-4 5.111990941552051E-5 0.0 0.04917735285773074 1.0223981883104103E-4 50-51 8.690384600638488E-4 5.111990941552051E-5 0.0 0.06781055983968796 1.0223981883104103E-4 52-53 8.690384600638488E-4 5.111990941552051E-5 0.0 0.08268645347960443 1.0223981883104103E-4 54-55 8.690384600638488E-4 5.111990941552051E-5 0.0 0.10438685502649289 1.0223981883104103E-4 56-57 9.712782788948898E-4 5.111990941552051E-5 0.0 0.12176762422776986 1.0223981883104103E-4 58-59 9.712782788948898E-4 5.111990941552051E-5 0.0 0.14178106876394614 1.0223981883104103E-4 60-61 9.712782788948898E-4 5.111990941552051E-5 0.0 0.1768493266229932 1.0223981883104103E-4 62-63 9.968382336026501E-4 5.111990941552051E-5 0.0 0.20197476210072157 1.0223981883104103E-4 64-65 0.0010223981883104103 7.667986412328077E-5 0.0 0.2305763514187053 1.0223981883104103E-4 66-67 0.0010735180977259307 1.0223981883104103E-4 0.0 0.2922014022191153 1.0223981883104103E-4 68-69 0.0011246380071414513 1.0223981883104103E-4 0.0 0.35809496545572117 1.0223981883104103E-4 70-71 0.0011246380071414513 1.0223981883104103E-4 0.0 0.4117964302967255 1.0223981883104103E-4 72-73 0.0011501979618492118 1.0223981883104103E-4 0.0 0.47339592114242773 1.0223981883104103E-4 74-75 0.001175757916556972 1.0223981883104103E-4 0.0 0.5254871088368431 1.0223981883104103E-4 76-77 0.001175757916556972 1.0223981883104103E-4 0.0 0.5689901517494511 1.0223981883104103E-4 78-79 0.001175757916556972 1.0223981883104103E-4 0.0 0.6295161244974274 1.0223981883104103E-4 80-81 0.001175757916556972 1.0223981883104103E-4 0.0 0.728382029307044 1.0223981883104103E-4 82-83 0.001175757916556972 1.0223981883104103E-4 0.0 0.8262255359283504 1.0223981883104103E-4 84-85 0.0012268778259724926 1.0223981883104103E-4 0.0 0.9429067291692759 1.0223981883104103E-4 86-87 0.001277997735388013 1.0223981883104103E-4 0.0 1.152063838542878 1.0223981883104103E-4 88-89 0.001277997735388013 1.0223981883104103E-4 0.0 1.4174784082282605 1.0223981883104103E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2060 0.0 29.977182 2 GTACCTG 2295 0.0 28.231989 1 GTACATG 8620 0.0 26.892443 1 TACATGG 8630 0.0 26.035446 2 GTATCAA 13655 0.0 25.236153 1 ACATGGG 8440 0.0 24.87489 3 CATGGGG 4010 0.0 22.150248 4 GGTATCA 10025 0.0 21.735111 1 TAACGCA 155 3.909078E-5 21.451006 4 ATCAACG 16085 0.0 21.409094 3 GAGTACT 8645 0.0 21.368105 12-13 TCAACGC 16160 0.0 21.309732 4 TATCAAC 16185 0.0 21.2491 2 CAACGCA 16350 0.0 21.059404 5 AACGCAG 16725 0.0 20.558825 6 AGTACTT 9115 0.0 20.162098 12-13 GTACTTT 9350 0.0 19.756927 14-15 CATGGGA 4565 0.0 19.145134 4 ACGCAGA 18295 0.0 18.768593 7 CGCAGAG 18345 0.0 18.69155 8 >>END_MODULE