FastQCFastQC Report
Thu 26 May 2016
SRR936441_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936441_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943655
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62790.3230511587704608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53110.27324808157826364No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30980.15939042679899468No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29130.14987227671577516No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT19570.10068659304248953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20000.028.7499982
GTACCTG20750.028.625431
GTACATG85300.025.1239321
GTATCAA132100.024.8202631
TACATGG86850.024.2390922
GAGTACT67150.022.82500512-13
ACATGGG88350.022.5904673
GGTATCA97200.021.5591891
GTACTTT71950.021.33365814-15
TCAACGC152350.021.1501944
ATCAACG152750.021.092643
TATCAAC153700.020.9931852
AGTACTT68150.020.95587512-13
CAACGCA153950.020.9287665
AACGCAG155450.020.8780576
CATGGGG41600.019.42154
ACGCAGA169350.019.1107587
CGCAGAG170900.018.8813298
GTCTACG1300.007548253518.2762341
GTGTAAG8650.018.1283361
Produced by FastQC (version 0.11.5)