##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936441_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943655 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.254165476897906 31.0 31.0 34.0 28.0 34.0 2 31.284860224679793 31.0 31.0 34.0 28.0 34.0 3 31.334043335880082 31.0 31.0 34.0 28.0 34.0 4 34.962515466993885 37.0 35.0 37.0 32.0 37.0 5 34.93156861685844 37.0 35.0 37.0 32.0 37.0 6 35.061596836887205 37.0 35.0 37.0 32.0 37.0 7 34.92004908278475 37.0 35.0 37.0 32.0 37.0 8 34.86076952957186 37.0 35.0 37.0 32.0 37.0 9 36.321176340451366 39.0 37.0 39.0 32.0 39.0 10-11 36.22519994546357 39.0 35.0 39.0 32.0 39.0 12-13 36.11316746027458 39.0 35.0 39.0 31.0 39.0 14-15 37.15370191726413 39.0 36.5 41.0 31.0 41.0 16-17 37.01585363657645 39.0 36.0 41.0 31.0 41.0 18-19 36.95896468251824 39.0 36.0 41.0 31.0 41.0 20-21 36.870350962490775 39.0 36.0 41.0 30.5 41.0 22-23 36.73859095364146 39.0 36.0 41.0 30.0 41.0 24-25 36.87351716225359 39.0 36.0 41.0 30.0 41.0 26-27 36.84975137048499 39.0 36.0 41.0 30.0 41.0 28-29 36.74421129264195 39.0 36.0 41.0 30.0 41.0 30-31 36.55314086090381 39.0 36.0 41.0 30.0 41.0 32-33 36.37737612899409 39.0 35.0 40.5 30.0 41.0 34-35 36.15437822041463 39.0 35.0 40.0 29.5 41.0 36-37 35.95030187970602 38.0 35.0 40.0 29.0 41.0 38-39 35.73889682068062 38.0 35.0 40.0 27.5 41.0 40-41 35.55690799035837 38.0 35.0 40.0 27.0 41.0 42-43 35.32738860548811 38.0 34.0 40.0 26.5 41.0 44-45 35.065238172412286 38.0 34.0 40.0 25.5 41.0 46-47 34.7761750413525 38.0 34.0 40.0 24.0 41.0 48-49 34.65984729800299 38.0 34.0 40.0 24.0 41.0 50-51 33.74130182568409 37.5 32.5 39.5 23.5 40.5 52-53 33.875309918684124 37.5 33.0 39.5 23.0 40.5 54-55 34.290814727922395 38.0 33.5 40.0 23.0 41.0 56-57 34.201647154458996 38.0 33.5 40.0 23.0 41.0 58-59 33.93833653606221 38.0 33.0 40.0 22.5 41.0 60-61 33.6159390426799 37.0 33.0 40.0 22.0 41.0 62-63 33.27446306057402 37.0 32.5 40.0 20.5 41.0 64-65 32.92866686731956 36.0 32.0 39.0 20.0 41.0 66-67 32.53801626317428 36.0 31.5 39.0 18.5 41.0 68-69 32.132301257167555 35.0 31.0 39.0 17.5 40.0 70-71 31.697796419632084 35.0 31.0 38.0 14.0 40.0 72-73 31.307743658210946 35.0 31.0 37.0 12.0 39.5 74-75 30.87454357897878 35.0 30.0 37.0 8.0 39.0 76-77 30.441291021297502 34.5 30.0 36.0 7.0 39.0 78-79 29.99507191348259 34.0 29.5 36.0 4.5 38.5 80-81 29.52378868677826 34.0 29.0 35.0 2.0 37.0 82-83 29.038397246424907 34.0 29.0 35.0 2.0 37.0 84-85 28.72503170572967 34.0 29.0 35.0 2.0 36.0 86-87 28.393738086234443 34.0 28.0 35.0 2.0 36.0 88-89 27.992650187404656 34.0 27.0 35.0 2.0 35.5 90-91 27.589039207060924 33.0 26.5 35.0 2.0 35.0 92-93 27.216119373036882 33.0 26.0 35.0 2.0 35.0 94-95 26.75100982427437 33.0 25.0 35.0 2.0 35.0 96-97 26.156033092292613 33.0 24.5 35.0 2.0 35.0 98-99 25.436928878839097 32.5 21.0 35.0 2.0 35.0 100-101 22.969584622785423 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37645346313162875 1101 2 0.48471128204403513 1101 3 0.49904638635068466 1101 4 0.6157685324602227 1101 5 0.45870780931022637 1101 6 0.3645825088035579 1101 7 0.4101905509873518 1101 8 0.508413012944672 1101 9 0.7365636516596368 1101 10-11 0.67862923498312 1101 12-13 0.648037077029322 1101 14-15 0.8917866833802464 1101 16-17 0.9172580561976034 1101 18-19 0.950236919136934 1101 20-21 0.863796792331641 1101 22-23 0.9025167407772159 1101 24-25 0.8421049373965914 1101 26-27 0.8063794466837493 1101 28-29 0.8145719115303365 1101 30-31 0.9722699853630274 1101 32-33 0.7671269771471572 1101 34-35 0.7357144724626394 1101 36-37 0.6721229452784385 1101 38-39 0.5548584191928256 1101 40-41 0.7099756199641192 1101 42-43 0.7723077048993545 1101 44-45 0.667380479446706 1101 46-47 0.619449216651212 1101 48-49 0.6078664551763353 1101 50-51 0.716734830547459 1101 52-53 0.7702455772371124 1101 54-55 0.81043779346831 1101 56-57 0.7084207403827705 1101 58-59 0.6940694350097303 1101 60-61 0.6673130930874507 1101 62-63 0.6867554081819449 1101 64-65 0.6352803564213119 1101 66-67 0.8463437763588431 1101 68-69 0.6681335085119215 1101 70-71 0.6344505349728884 1101 72-73 0.5349183632136807 1101 74-75 0.5190994488587855 1101 76-77 0.46802526276126244 1101 78-79 0.5630685502004518 1101 80-81 0.6392298246347785 1101 82-83 0.7174159022027844 1101 84-85 0.8582175384487378 1101 86-87 0.7803185121311706 1101 88-89 0.727631511976476 1101 90-91 0.5656324064766203 1101 92-93 0.4510022577828181 1101 94-95 0.7412258251788053 1101 96-97 0.6021721155827962 1101 98-99 0.5979621324385853 1101 100-101 0.9120265964476282 1102 1 0.5147185344615899 1102 2 0.4249735922143074 1102 3 0.38298564083757114 1102 4 0.5255361348532261 1102 5 0.4754059225177727 1102 6 0.362978735218654 1102 7 0.4742816692570315 1102 8 0.42867762408135235 1102 9 0.7154315761879388 1102 10-11 0.6314893362256413 1102 12-13 0.5221903213919532 1102 14-15 0.674836614351257 1102 16-17 0.7052907299205629 1102 18-19 0.6837033250274018 1102 20-21 0.7375864840021364 1102 22-23 0.5440100679746465 1102 24-25 0.35146066680294297 1102 26-27 0.3229820697854464 1102 28-29 0.3669568632100422 1102 30-31 0.4345249324408016 1102 32-33 0.39027239822400617 1102 34-35 0.2397457656057611 1102 36-37 0.23861742295906652 1102 38-39 0.18142077538242773 1102 40-41 0.10656328678417992 1102 42-43 0.3978702911855976 1102 44-45 0.15921204870579686 1102 46-47 0.1507665199185837 1102 48-49 0.4589996811312389 1102 50-51 0.6457845314218176 1102 52-53 0.593188513270249 1102 54-55 0.7022026347016279 1102 56-57 0.8105295760937707 1102 58-59 0.7891614736659704 1102 60-61 0.7374361947901704 1102 62-63 0.7151746442610971 1102 64-65 0.7247177701350722 1102 66-67 0.7612356309377688 1102 68-69 0.6254943000443589 1102 70-71 0.6659197020230501 1102 72-73 0.5108709633057167 1102 74-75 0.5483378289784362 1102 76-77 0.6597947059273928 1102 78-79 0.5150174687462403 1102 80-81 0.670624210276749 1102 82-83 0.8068728741309918 1102 84-85 0.7102561946843515 1102 86-87 0.6568969750856084 1102 88-89 0.7695528189253942 1102 90-91 0.7800571648751493 1102 92-93 0.7333722531808817 1102 94-95 0.8297727648934874 1102 96-97 0.7052680658819206 1102 98-99 0.8019013869254721 1102 100-101 1.0913869277867931 1103 1 0.2632776788314146 1103 2 0.38315324612469226 1103 3 0.27147300892199766 1103 4 0.3786710174172754 1103 5 0.5528132707038225 1103 6 0.3471248816849126 1103 7 0.34738865856164836 1103 8 0.3825125030921157 1103 9 0.6297086610758313 1103 10-11 0.6030621962618881 1103 12-13 0.44898650516767447 1103 14-15 0.29714633612931607 1103 16-17 0.5243576382103754 1103 18-19 0.39507959976712215 1103 20-21 0.4035877987162735 1103 22-23 0.4787238511534042 1103 24-25 0.5007660002784036 1103 26-27 0.5154433390175868 1103 28-29 0.14829636148670033 1103 30-31 0.14594873231998662 1103 32-33 0.396115769577456 1103 34-35 0.30232349363760136 1103 36-37 0.40562209552418693 1103 38-39 0.4826368859605452 1103 40-41 0.24138988664915217 1103 42-43 0.4447536191890151 1103 44-45 0.5055264075161574 1103 46-47 0.5840514397920913 1103 48-49 0.546599173011991 1103 50-51 0.4598093333263833 1103 52-53 0.5916357292742234 1103 54-55 0.6243271416838283 1103 56-57 0.5091930981063513 1103 58-59 0.3071313063064096 1103 60-61 0.22052746082434993 1103 62-63 0.2975867893771138 1103 64-65 0.40335233290378625 1103 66-67 0.38874513596565663 1103 68-69 0.33832964098171914 1103 70-71 0.6130144502730595 1103 72-73 0.5547895220947616 1103 74-75 0.46305907404825675 1103 76-77 0.40938785520074816 1103 78-79 0.6237878556985912 1103 80-81 0.5666925043463209 1103 82-83 0.2860062287840144 1103 84-85 0.48818676356471613 1103 86-87 0.6144699061874839 1103 88-89 0.6341799020366494 1103 90-91 0.7791510781040181 1103 92-93 0.5818887581732426 1103 94-95 0.5787241027039549 1103 96-97 0.7001455665037462 1103 98-99 0.7767823519967116 1103 100-101 1.104742824457368 1104 1 0.3441688088010366 1104 2 0.33741743767702204 1104 3 0.37065650328403166 1104 4 0.3605471258772255 1104 5 0.4286846932676909 1104 6 0.22105037750443302 1104 7 0.4593264620413322 1104 8 0.49786939128099306 1104 9 0.5879182517521002 1104 10-11 0.5864930871306626 1104 12-13 0.4862621032462968 1104 14-15 0.5296437698568042 1104 16-17 0.4038019259358805 1104 18-19 0.6916688734009924 1104 20-21 0.7898555634602644 1104 22-23 0.5425975905454905 1104 24-25 0.6679602873509225 1104 26-27 0.2235105766635641 1104 28-29 0.4822249002654999 1104 30-31 0.4600402457285995 1104 32-33 0.29170406632997725 1104 34-35 0.23834135698391634 1104 36-37 0.3089330441699971 1104 38-39 0.39503263522070853 1104 40-41 0.4053361738470471 1104 42-43 0.44877737777916593 1104 44-45 0.6483571040534599 1104 46-47 0.5662517605435085 1104 48-49 0.4806289914008701 1104 50-51 0.38908240561821117 1104 52-53 0.5627540625161416 1104 54-55 0.5133417322164391 1104 56-57 0.36812744854555746 1104 58-59 0.5092517134621986 1104 60-61 0.5688267992092548 1104 62-63 0.8073921705828582 1104 64-65 0.5348254778087238 1104 66-67 0.6666561248360026 1104 68-69 0.7387256174843273 1104 70-71 0.714585622870235 1104 72-73 0.8185073261490778 1104 74-75 0.5899259883308403 1104 76-77 0.7689418984186069 1104 78-79 0.6735030190389004 1104 80-81 0.5446081947846402 1104 82-83 0.5594396271557045 1104 84-85 0.5875047750104194 1104 86-87 0.7781465062224839 1104 88-89 0.7024724623277301 1104 90-91 0.7766758984649407 1104 92-93 0.7292933620530846 1104 94-95 0.7782498320346853 1104 96-97 0.8814876552014397 1104 98-99 1.0491654070233416 1104 100-101 1.3378410140925006 1105 1 0.431421106397206 1105 2 0.326231306696787 1105 3 0.39041449984464194 1105 4 0.47955980584323754 1105 5 0.5438458532971211 1105 6 0.4017654755794098 1105 7 0.3879629011020569 1105 8 0.5299960390697365 1105 9 0.6099936937747543 1105 10-11 0.4841511085921226 1105 12-13 0.4449508038174983 1105 14-15 0.3380747325031379 1105 16-17 0.43135155403477654 1105 18-19 0.29447479534816523 1105 20-21 0.3872092579490385 1105 22-23 0.35591288536102894 1105 24-25 0.37311684147913127 1105 26-27 0.37654916260645876 1105 28-29 0.21447752345496696 1105 30-31 0.24419860652101733 1105 32-33 0.46321255414705576 1105 34-35 0.49396389894379666 1105 36-37 0.5828739410897583 1105 38-39 0.3579312246986106 1105 40-41 0.044480795900291525 1105 42-43 0.4610854535097886 1105 44-45 0.32372057326412573 1105 46-47 0.5420921968947781 1105 48-49 0.6843790041109017 1105 50-51 0.7614031026864225 1105 52-53 0.5605998463857915 1105 54-55 0.6340664271809473 1105 56-57 0.7264563955638437 1105 58-59 0.8724388153811233 1105 60-61 0.48614459838893964 1105 62-63 0.4894771402659046 1105 64-65 0.8134094866129402 1105 66-67 0.9077158845113686 1105 68-69 0.8384164444718394 1105 70-71 0.9617429553935786 1105 72-73 0.7810290494960235 1105 74-75 0.8368561627385134 1105 76-77 0.6265482662879762 1105 78-79 0.6671561131163273 1105 80-81 0.5362341731059637 1105 82-83 0.6177698289521594 1105 84-85 0.7431898611714942 1105 86-87 0.9423489400161671 1105 88-89 0.7794328747257175 1105 90-91 0.6934618231423393 1105 92-93 0.9304047709937251 1105 94-95 0.9512204609230075 1105 96-97 0.9996687201230117 1105 98-99 0.9274865398024552 1105 100-101 1.3915454112894992 1106 1 0.21237491117132734 1106 2 0.28236217775070216 1106 3 0.29394237255983313 1106 4 0.27129535706944097 1106 5 0.30984439001700537 1106 6 0.32471622418082546 1106 7 0.49571667673571795 1106 8 0.4329066882539152 1106 9 0.7194862877627912 1106 10-11 0.6099826955091316 1106 12-13 0.6944214039098355 1106 14-15 0.8693886588269351 1106 16-17 0.8278849343861552 1106 18-19 0.7135453264330422 1106 20-21 0.5784106717082551 1106 22-23 0.5333087830815941 1106 24-25 0.49004259900816294 1106 26-27 0.3593381905978319 1106 28-29 0.6722705627288832 1106 30-31 0.6048129070673198 1106 32-33 0.4416460444008692 1106 34-35 0.45264064946079685 1106 36-37 0.5063268262857363 1106 38-39 0.508383706308166 1106 40-41 0.41032831174317863 1106 42-43 0.18367677331783483 1106 44-45 0.35621967156356504 1106 46-47 0.31627196224256693 1106 48-49 0.2984702709566207 1106 50-51 0.3173812768399955 1106 52-53 0.4953794090911927 1106 54-55 0.5591267305009424 1106 56-57 0.4209648268551547 1106 58-59 0.5095093679190938 1106 60-61 0.4428764914148431 1106 62-63 0.6274086101294358 1106 64-65 0.49753115428745787 1106 66-67 0.4243701932992323 1106 68-69 0.366943814380015 1106 70-71 0.5242816435991564 1106 72-73 0.5998921559315527 1106 74-75 0.6214555417606888 1106 76-77 0.7459298449589475 1106 78-79 0.8179382126506596 1106 80-81 0.8711405102172769 1106 82-83 0.9668943190499881 1106 84-85 0.7725248508628333 1106 86-87 0.9760221485371616 1106 88-89 1.1459656606474766 1106 90-91 1.2407827780376444 1106 92-93 1.2913384098549372 1106 94-95 1.2949006685174957 1106 96-97 1.4908321663666833 1106 98-99 1.6997253583803023 1106 100-101 2.115913427579553 1107 1 0.09528038707554742 1107 2 0.13103155615250728 1107 3 0.008964784980143747 1107 4 0.26659130871016146 1107 5 0.20440097012352965 1107 6 0.2297026566778655 1107 7 0.24889890498401712 1107 8 0.35400022722716784 1107 9 0.21643426164115454 1107 10-11 0.3476761670420956 1107 12-13 0.3314167713623064 1107 14-15 0.26547632767041307 1107 16-17 0.24257319077223372 1107 18-19 0.43929264250378 1107 20-21 0.4817892182817758 1107 22-23 0.49694575660156914 1107 24-25 0.3999217626073559 1107 26-27 0.29648719655976663 1107 28-29 0.20416390467762113 1107 30-31 0.27657930414937226 1107 32-33 0.2775295739642516 1107 34-35 0.5179648218607795 1107 36-37 0.4502150060511738 1107 38-39 0.4663621836759475 1107 40-41 0.26164123467940925 1107 42-43 0.40151265508194456 1107 44-45 0.23143417405020017 1107 46-47 0.3609184921572819 1107 48-49 0.33107663506002183 1107 50-51 0.2637953778627278 1107 52-53 0.38488017441488154 1107 54-55 0.388670740585205 1107 56-57 0.38565643760111357 1107 58-59 0.4293403876268371 1107 60-61 0.45760455875626604 1107 62-63 0.465857342269679 1107 64-65 0.6452898239836387 1107 66-67 0.5438752221908629 1107 68-69 0.547252574379101 1107 70-71 0.7705508009663085 1107 72-73 0.6412375102314023 1107 74-75 0.6652727503330205 1107 76-77 0.5611322011319402 1107 78-79 0.6757435873944466 1107 80-81 0.7104761615898312 1107 82-83 0.9595862281574306 1107 84-85 0.8572385474203763 1107 86-87 0.7182342959100794 1107 88-89 0.7705450415738788 1107 90-91 0.718518435059643 1107 92-93 0.7517362192813977 1107 94-95 0.6950680775569644 1107 96-97 0.7966811548506385 1107 98-99 1.1940542833785805 1107 100-101 1.5380353426718791 1108 1 0.09028353549828694 1108 2 0.11827426201210756 1108 3 0.11077188248763292 1108 4 0.21980521687866172 1108 5 0.3734999736676059 1108 6 0.17917400426461683 1108 7 0.13996016631370622 1108 8 0.14684262151213545 1108 9 0.24075858715892196 1108 10-11 0.059492157850286276 1108 12-13 0.31066290295313337 1108 14-15 0.20718038829675578 1108 16-17 0.41117354685752616 1108 18-19 0.38252002309553745 1108 20-21 0.3894207653893318 1108 22-23 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2316 78-79 -3.2038289087576253 2316 80-81 -2.8575078963472755 2316 82-83 -2.7786817930064203 2316 84-85 -2.6703618674333818 2316 86-87 -3.2604291689835065 2316 88-89 -3.5812013269297864 2316 90-91 -3.56341668274845 2316 92-93 -4.20243734381188 2316 94-95 -4.545723984880663 2316 96-97 -5.541540248673485 2316 98-99 -6.678780018252045 2316 100-101 -6.864581857584074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4932.0 3 2043.0 4 1994.0 5 2213.0 6 2482.0 7 2928.0 8 3698.0 9 4502.0 10 5856.0 11 7360.0 12 8828.0 13 10735.0 14 11867.0 15 13839.0 16 15990.0 17 19471.0 18 17178.0 19 13318.0 20 13647.0 21 14609.0 22 16379.0 23 18659.0 24 21048.0 25 23863.0 26 28116.0 27 33336.0 28 39417.0 29 46574.0 30 57074.0 31 69854.0 32 85598.0 33 108789.0 34 141570.0 35 190459.0 36 261578.0 37 334606.0 38 253158.0 39 36074.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.009328478235695 18.14547279301072 13.963483832782202 22.881714895971385 2 15.747053326252258 19.94027402110325 40.12010037010963 24.19257228253486 3 19.75336024029983 24.527708634913154 29.23304853391683 26.48588259087019 4 12.776995940738697 16.300461518346975 37.019371828541246 33.903170712373075 5 13.856703675943697 37.02928482592187 33.78750689805701 15.326504600077422 6 31.8529129866774 35.445979181701475 17.879471708136144 14.82163612348498 7 29.087244901898714 32.41082344341577 20.338303386894605 18.163628267790912 8 26.783516584765003 33.94496640633485 19.711835172024376 19.55968183687577 9 26.783064420242525 16.90557184629979 20.375937875903134 35.935425857554556 10-11 25.28151979248227 26.268891747628544 26.967935957368063 21.481652502521133 12-13 26.422716415167713 23.703974610835278 27.440414038369433 22.432894935627576 14-15 23.182993836295086 25.17859302819348 26.016651010770396 25.621762124741032 16-17 22.391264285927544 28.413231232041248 26.659411956681762 22.536092525349446 18-19 22.288630052786505 26.856742351001934 29.04083927142888 21.81378832478268 20-21 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28.246910772691418 22.640819218268696 46-47 22.884411960214937 26.740236380736388 27.760382045271665 22.61496961377701 48-49 22.933696090338017 26.797336020396852 27.56980675626346 22.69916113300167 50-51 22.761218890722493 27.058103236046566 27.648666621219586 22.532011252011355 52-53 23.07466714344699 26.999975560720674 27.32944277156863 22.5959145242637 54-55 22.622904764918445 26.926983401740245 27.534130136242695 22.915981697098612 56-57 22.80805213163201 27.234954354976033 27.211000398031914 22.745993115360044 58-59 23.04184610777349 26.793328354134736 27.258436615685195 22.906388922406574 60-61 22.795909892915635 26.851232954671172 27.79446656180004 22.55839059061315 62-63 22.716161245077245 26.945150206377484 27.768241844976604 22.57044670356867 64-65 22.56823422063952 27.042589102126946 27.51885687502589 22.870319802207646 66-67 22.51166380458062 27.230491185418153 27.2899453952573 22.96789961474392 68-69 22.599030828344848 27.03352889324343 27.51217125017174 22.855269028239977 70-71 22.66471593373545 27.011108063984178 27.28113038919129 23.043045613089085 72-73 22.71404051442578 26.912503781634527 27.397196136661584 22.97625956727811 74-75 22.57461952230849 27.50347104937727 27.081336906471137 22.840572521843107 76-77 22.78310729650833 27.19151356645436 27.085031814607014 22.940347322430295 78-79 22.748199691699135 27.255170419581425 27.1598811662746 22.836748722444838 80-81 22.78616357540876 27.475881344886925 26.935480426835685 22.802474652868625 82-83 22.792208226547782 27.446325951088145 27.043169232178236 22.718296590185833 84-85 22.70972303483793 27.390595338990682 26.983018175661872 22.916663450509517 86-87 22.82433563332058 27.330804813740134 27.050057330318594 22.79480222262069 88-89 22.80387079678574 27.271186026439036 26.868850582253145 23.056092594522074 90-91 22.721998951419007 27.305141699925755 27.0070318726432 22.965827476012038 92-93 22.822200455354636 27.214656563340938 27.15897866563956 22.804164315664863 94-95 22.595660940307123 27.13585887289005 27.197167701901705 23.071312484901124 96-97 22.549579808151194 27.040956645565956 27.257760974963436 23.15170257131941 98-99 22.436890376007483 27.203960877024873 26.939681124903895 23.419467622063745 100-101 22.487943466825875 27.09868600515716 27.034942072025242 23.378428455991724 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 131.0 1 116.0 2 210.0 3 321.5 4 277.0 5 251.0 6 387.5 7 783.0 8 1262.5 9 1519.5 10 1510.5 11 1482.0 12 1591.0 13 1742.5 14 1919.5 15 2094.5 16 2158.0 17 2281.0 18 2534.0 19 2746.0 20 3092.5 21 3613.5 22 4253.5 23 4987.5 24 5941.5 25 7347.0 26 9364.0 27 11885.5 28 14403.0 29 17260.0 30 21020.5 31 25581.5 32 30549.5 33 34119.5 34 37133.0 35 40131.5 36 43667.5 37 47963.5 38 50381.0 39 51867.5 40 53745.5 41 57060.5 42 60005.5 43 62751.0 44 66193.5 45 69345.5 46 71103.0 47 73940.5 48 82740.0 49 82597.0 50 74111.0 51 70249.0 52 67737.0 53 66814.5 54 65825.5 55 61470.5 56 55897.5 57 50853.5 58 47329.0 59 42809.0 60 35851.5 61 29668.5 62 24803.0 63 19578.0 64 14410.5 65 10909.5 66 8255.5 67 6633.0 68 5178.5 69 3687.0 70 2787.0 71 2196.5 72 1744.0 73 1468.0 74 1228.0 75 958.0 76 686.0 77 427.0 78 260.5 79 169.0 80 118.0 81 77.5 82 42.0 83 28.0 84 22.0 85 17.5 86 14.0 87 11.5 88 8.0 89 5.0 90 2.5 91 1.5 92 2.5 93 3.5 94 2.0 95 0.5 96 0.5 97 2.0 98 1.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03498563273832033 2 0.022997908579454687 3 0.021402975322266555 4 0.048208143935009044 5 0.05551396724212888 6 0.048413941774646224 7 0.0524784491074805 8 0.04517262580036066 9 0.0429088495643517 10-11 0.055256719942582404 12-13 0.048182419205054394 14-15 0.042703051724714516 16-17 0.051912505048478254 18-19 0.05726324887904489 20-21 0.013248235926643358 22-23 0.022431964520452447 24-25 0.006585530868389708 26-27 0.01286236497732365 28-29 0.007100025467482654 30-31 0.009826846842675268 32-33 0.0044761030121086304 34-35 0.00923517805371838 36-37 0.010727212391087926 38-39 0.012502218757958589 40-41 0.0019293547465985476 42-43 0.0020065289364624896 44-45 0.0023666751558275518 46-47 5.659440590022406E-4 48-49 0.0011061633880498339 50-51 1.286236497732365E-4 52-53 0.0032155912443309126 54-55 0.007100025467482654 56-57 0.017672889478842695 58-59 0.012167797268548173 60-61 0.012039173618774938 62-63 0.006636980328299004 64-65 2.5724729954647298E-5 66-67 0.007331548037074481 68-69 0.017801513128615932 70-71 0.005762339509840996 72-73 0.002623922455374025 74-75 0.004038782602879627 76-77 0.006662705058253652 78-79 0.005093496531020166 80-81 0.009801122112720622 82-83 0.006071036269296763 84-85 0.017595715288978755 86-87 0.004810524501519046 88-89 0.0065598061384350625 90-91 0.003910158953106389 92-93 0.017209844339659046 94-95 0.011087358610452987 96-97 0.010161268332085683 98-99 0.023100807499273276 100-101 0.03730085843423859 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.83180503864416 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14457079607297 49.36170710611169 2 11.348382674954012 13.80685204773459 3 3.2180677270515967 5.872826057194665 4 1.382500455860548 3.3639999278698216 5 0.7197106019346716 2.1890647510567587 6 0.4258291219639753 1.5542372476253743 7 0.30684438238030837 1.3066128352313469 8 0.22429590235943805 1.091545968263688 9 0.16340164349735958 0.8946015228202873 >10 0.9513338476267489 10.87356909557496 >50 0.07461555048925678 3.1344279690623256 >100 0.03721937748619121 4.164315216125726 >500 0.0023784661326949623 1.010376052154564 >1k 6.795617521985599E-4 0.7769659039633823 >5k 1.6989043804963998E-4 0.5988982992109337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6279 0.3230511587704608 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5311 0.27324808157826364 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3098 0.15939042679899468 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2913 0.14987227671577516 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1957 0.10068659304248953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.14494599092946E-5 0.0 3 0.0 0.0 0.0 5.14494599092946E-5 0.0 4 0.0 0.0 0.0 5.14494599092946E-5 0.0 5 0.0 0.0 0.0 5.14494599092946E-5 0.0 6 0.0 0.0 0.0 5.14494599092946E-5 0.0 7 0.0 0.0 0.0 5.14494599092946E-5 0.0 8 0.0 0.0 0.0 5.14494599092946E-5 0.0 9 0.0 0.0 0.0 5.14494599092946E-5 1.028989198185892E-4 10-11 5.14494599092946E-5 0.0 0.0 5.14494599092946E-5 1.028989198185892E-4 12-13 5.14494599092946E-5 0.0 0.0 7.71741898639419E-5 1.028989198185892E-4 14-15 5.14494599092946E-5 0.0 0.0 1.028989198185892E-4 1.028989198185892E-4 16-17 5.14494599092946E-5 0.0 0.0 1.543483797278838E-4 1.028989198185892E-4 18-19 1.028989198185892E-4 0.0 0.0 1.8007310968253112E-4 1.028989198185892E-4 20-21 1.543483797278838E-4 5.14494599092946E-5 0.0 2.057978396371784E-4 1.028989198185892E-4 22-23 1.543483797278838E-4 7.71741898639419E-5 0.0 2.057978396371784E-4 1.028989198185892E-4 24-25 2.57247299546473E-4 1.028989198185892E-4 0.0 2.057978396371784E-4 1.028989198185892E-4 26-27 2.57247299546473E-4 1.028989198185892E-4 0.0 2.057978396371784E-4 1.028989198185892E-4 28-29 2.57247299546473E-4 1.028989198185892E-4 0.0 4.630451391836514E-4 1.028989198185892E-4 30-31 2.57247299546473E-4 1.543483797278838E-4 0.0 0.001466309607414896 1.028989198185892E-4 32-33 2.8297202950112034E-4 1.543483797278838E-4 0.0 0.00257247299546473 1.028989198185892E-4 34-35 3.086967594557676E-4 1.543483797278838E-4 0.0 0.0038844342231517423 1.028989198185892E-4 36-37 3.6014621936506224E-4 1.543483797278838E-4 0.0 0.005299294370657345 1.028989198185892E-4 38-39 3.6014621936506224E-4 1.543483797278838E-4 0.0 0.00676560397807224 1.028989198185892E-4 40-41 3.6014621936506224E-4 1.543483797278838E-4 0.0 0.00866923399471614 1.028989198185892E-4 42-43 4.115956792743568E-4 1.543483797278838E-4 0.0 0.011756201589273816 1.028989198185892E-4 44-45 5.14494599092946E-4 1.543483797278838E-4 0.0 0.017106945419840456 1.028989198185892E-4 46-47 6.173935189115352E-4 1.8007310968253112E-4 0.0 0.028734523359341037 1.028989198185892E-4 48-49 6.173935189115352E-4 2.057978396371784E-4 0.0 0.05345598884575709 1.028989198185892E-4 50-51 6.173935189115352E-4 2.057978396371784E-4 0.0 0.07048576007573361 1.028989198185892E-4 52-53 6.173935189115352E-4 2.057978396371784E-4 0.0 0.08787567752507519 1.028989198185892E-4 54-55 6.173935189115352E-4 2.57247299546473E-4 0.0 0.11318881180044812 1.028989198185892E-4 56-57 7.202924387301245E-4 2.57247299546473E-4 0.0 0.1312990216885198 1.028989198185892E-4 58-59 7.202924387301245E-4 2.57247299546473E-4 0.0 0.15313931742001538 1.028989198185892E-4 60-61 7.202924387301245E-4 2.57247299546473E-4 0.0 0.19054307477407256 1.028989198185892E-4 62-63 7.202924387301245E-4 2.57247299546473E-4 0.0 0.21845440677486488 1.028989198185892E-4 64-65 7.202924387301245E-4 2.57247299546473E-4 0.0 0.24824364406234645 1.028989198185892E-4 66-67 7.202924387301245E-4 2.57247299546473E-4 0.0 0.3072047251183981 1.028989198185892E-4 68-69 7.202924387301245E-4 2.57247299546473E-4 0.0 0.3747835907092565 1.028989198185892E-4 70-71 7.202924387301245E-4 2.57247299546473E-4 0.0 0.4302975579513854 1.028989198185892E-4 72-73 7.460171686847717E-4 2.57247299546473E-4 0.0 0.4899532067162125 1.028989198185892E-4 74-75 7.71741898639419E-4 2.57247299546473E-4 0.0 0.5394218624189993 1.028989198185892E-4 76-77 7.71741898639419E-4 2.57247299546473E-4 0.0 0.5804785314266163 1.028989198185892E-4 78-79 7.71741898639419E-4 2.57247299546473E-4 0.0 0.6401084554614888 1.028989198185892E-4 80-81 8.48916088503361E-4 2.57247299546473E-4 0.0 0.7410265710735702 1.028989198185892E-4 82-83 8.746408184580083E-4 2.57247299546473E-4 0.0 0.8403497534284634 1.028989198185892E-4 84-85 8.746408184580083E-4 2.57247299546473E-4 0.0 0.9619248271941265 1.028989198185892E-4 86-87 8.746408184580083E-4 2.57247299546473E-4 0.0 1.1826430102050005 1.028989198185892E-4 88-89 8.746408184580083E-4 2.57247299546473E-4 0.0 1.4509776683619264 1.028989198185892E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2000 0.0 28.749998 2 GTACCTG 2075 0.0 28.62543 1 GTACATG 8530 0.0 25.123932 1 GTATCAA 13210 0.0 24.820263 1 TACATGG 8685 0.0 24.239092 2 GAGTACT 6715 0.0 22.825005 12-13 ACATGGG 8835 0.0 22.590467 3 GGTATCA 9720 0.0 21.559189 1 GTACTTT 7195 0.0 21.333658 14-15 TCAACGC 15235 0.0 21.150194 4 ATCAACG 15275 0.0 21.09264 3 TATCAAC 15370 0.0 20.993185 2 AGTACTT 6815 0.0 20.955875 12-13 CAACGCA 15395 0.0 20.928766 5 AACGCAG 15545 0.0 20.878057 6 CATGGGG 4160 0.0 19.4215 4 ACGCAGA 16935 0.0 19.110758 7 CGCAGAG 17090 0.0 18.881329 8 GTCTACG 130 0.0075482535 18.276234 1 GTGTAAG 865 0.0 18.128336 1 >>END_MODULE