FastQCFastQC Report
Thu 26 May 2016
SRR936441_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936441_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943655
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73600.37866802493240825No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67440.3469751576282828No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37470.1927811262801269No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35210.1811535483406263No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.10650038201223982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG18500.032.3540762
GTACCTG20200.030.3515511
GTACATG81850.023.4007031
TACATGG83250.022.938872
ACATGGG82750.021.9844823
CGTATAG1302.931138E-421.9356461
GTATCAA146350.021.3685821
GAGTACT82500.020.98597112-13
AGTACTT85700.020.06380312-13
GGTATCA114550.019.5419711
GTACTTT88450.019.43999914-15
TATCACG1250.006029676619.00162
CATGGGG40900.018.4644744
TCAACGC173300.018.061344
ATCAACG173200.018.0452753
TATAGGG3701.4188117E-1017.9744852
CAACGCA175250.017.8874765
TATCAAC175400.017.820772
AACGCAG177500.017.714256
ACCTGGG33450.017.4660423
Produced by FastQC (version 0.11.5)