##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936441_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1943655 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.863887366842366 31.0 31.0 34.0 30.0 34.0 2 32.00513774306654 31.0 31.0 34.0 30.0 34.0 3 32.11214696023728 33.0 31.0 34.0 30.0 34.0 4 35.86162667757395 37.0 35.0 37.0 35.0 37.0 5 35.63893232080796 37.0 35.0 37.0 33.0 37.0 6 35.734842345992476 37.0 35.0 37.0 33.0 37.0 7 35.683831235481605 37.0 35.0 37.0 33.0 37.0 8 35.65760590228204 37.0 35.0 37.0 33.0 37.0 9 37.29017752636142 39.0 37.0 39.0 34.0 39.0 10-11 37.15855437307547 39.0 37.0 39.0 33.0 39.0 12-13 37.05989283077501 39.0 37.0 39.0 33.0 39.0 14-15 38.16151014454726 40.0 37.0 41.0 33.0 41.0 16-17 38.08503772531648 40.0 37.0 41.0 33.0 41.0 18-19 38.238046618355625 40.0 37.5 41.0 33.5 41.0 20-21 38.26989203330838 40.0 38.0 41.0 34.0 41.0 22-23 38.17726165394579 40.0 37.5 41.0 33.0 41.0 24-25 38.05584272929095 40.0 37.0 41.0 33.0 41.0 26-27 37.8366996714952 40.0 37.0 41.0 33.0 41.0 28-29 37.753688283157246 40.0 37.0 41.0 32.5 41.0 30-31 37.72043135227187 40.0 37.0 41.0 33.0 41.0 32-33 37.56837170176806 40.0 36.5 41.0 32.5 41.0 34-35 37.39190931518196 39.5 36.0 41.0 32.0 41.0 36-37 37.13106852810813 39.0 36.0 41.0 31.0 41.0 38-39 36.963087584988074 39.0 36.0 41.0 31.0 41.0 40-41 36.74901770118669 39.0 35.0 41.0 30.5 41.0 42-43 36.49872868384564 39.0 35.0 40.5 30.0 41.0 44-45 36.252238437377 39.0 35.0 40.0 30.0 41.0 46-47 35.891998579994905 39.0 35.0 40.0 29.0 41.0 48-49 35.523167691797155 38.0 35.0 40.0 27.5 41.0 50-51 35.20038149774523 38.0 34.5 40.0 27.0 41.0 52-53 34.91509681502119 38.0 34.0 40.0 26.0 41.0 54-55 34.650098654339374 38.0 34.0 40.0 26.0 41.0 56-57 34.328915882705516 38.0 33.5 40.0 25.0 41.0 58-59 33.98250615464164 37.0 33.0 40.0 24.0 41.0 60-61 33.61572809989427 37.0 33.0 39.5 23.0 41.0 62-63 33.209384895982055 36.0 32.0 39.0 22.5 40.0 64-65 32.78844007810028 36.0 31.5 39.0 22.0 40.0 66-67 32.32248084150737 35.0 31.0 38.5 20.5 40.0 68-69 32.03367470049983 35.0 31.0 38.0 20.0 40.0 70-71 31.602827662316614 35.0 31.0 37.5 18.5 39.5 72-73 31.149352637170693 34.5 30.0 37.0 18.0 39.0 74-75 30.623881553053398 34.0 30.0 36.0 14.0 39.0 76-77 29.201495378552263 32.5 28.0 35.0 12.0 37.0 78-79 29.980708767759708 34.0 29.0 35.0 11.0 37.0 80-81 29.78754588648706 34.0 29.0 35.0 7.5 37.0 82-83 29.45115336826752 34.0 29.0 35.0 7.0 36.5 84-85 29.033975679840303 34.0 29.0 35.0 2.0 36.0 86-87 28.646689613125787 33.5 29.0 35.0 2.0 36.0 88-89 28.23577872616282 33.0 27.5 35.0 2.0 35.0 90-91 27.746918048727785 33.0 27.0 35.0 2.0 35.0 92-93 27.241554699779538 33.0 26.0 34.5 2.0 35.0 94-95 26.645333662609875 32.0 25.0 34.0 2.0 35.0 96-97 25.85816387167476 32.0 24.5 34.0 2.0 35.0 98-99 24.876635256771394 32.0 19.0 34.0 2.0 35.0 100-101 21.578641271213257 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31019536280128435 1101 2 0.33848361404695737 1101 3 0.2880476682379438 1101 4 0.19226864979504654 1101 5 0.17397888809811235 1101 6 0.21149750487219166 1101 7 0.18660275713154562 1101 8 0.22804876816972808 1101 9 0.3987147848210739 1101 10-11 0.32918534429118296 1101 12-13 0.3600784623992297 1101 14-15 0.49880097637147003 1101 16-17 0.5225620113211207 1101 18-19 0.2525441955576966 1101 20-21 0.2639636435519179 1101 22-23 0.2315643268186065 1101 24-25 0.148742593127146 1101 26-27 0.3490907847281335 1101 28-29 0.25854215247823475 1101 30-31 0.3202491518296213 1101 32-33 0.42467173825681215 1101 34-35 0.22220852201348862 1101 36-37 0.32720621493734114 1101 38-39 0.22630582265934862 1101 40-41 0.22057282714928306 1101 42-43 0.34688897972775123 1101 44-45 0.24597793722532657 1101 46-47 0.27669294640244146 1101 48-49 0.26768400828576944 1101 50-51 0.09260445481010038 1101 52-53 0.21844545280775662 1101 54-55 0.2987151796727474 1101 56-57 0.04922390352477635 1101 58-59 0.09027095845390676 1101 60-61 -0.01567941585319943 1101 62-63 0.24712898582713905 1101 64-65 0.11958752382660265 1101 66-67 0.02707180190505909 1101 68-69 0.021763560297749507 1101 70-71 0.043661739857668636 1101 72-73 0.11092107595574063 1101 74-75 0.03953059890384125 1101 76-77 -0.05302446268672156 1101 78-79 -0.2871471023202794 1101 80-81 -0.053175133946517406 1101 82-83 0.09801881522934153 1101 84-85 -0.020568613816092096 1101 86-87 0.05353641723898406 1101 88-89 -0.01045329290543151 1101 90-91 -0.054633025871464014 1101 92-93 -0.1968560092659999 1101 94-95 -0.16444215167070908 1101 96-97 -0.00606315869761076 1101 98-99 0.09408639350280268 1101 100-101 0.5803643495045634 1102 1 0.22841671576953004 1102 2 0.3283616628274473 1102 3 0.21883689971516418 1102 4 0.16139658352721398 1102 5 0.09194023186249467 1102 6 0.20453247956156417 1102 7 0.2602060705231679 1102 8 0.19190058593318327 1102 9 0.2391588713374162 1102 10-11 0.3263804662423979 1102 12-13 0.26550759263393076 1102 14-15 0.4863895635780935 1102 16-17 0.39311309277533013 1102 18-19 0.2981712089033053 1102 20-21 0.1486207995574489 1102 22-23 0.12286437283793106 1102 24-25 0.1881800068409092 1102 26-27 -0.06284317753601698 1102 28-29 0.08993883908660649 1102 30-31 0.2198453322253897 1102 32-33 0.20288043590982596 1102 34-35 0.1372142744295033 1102 36-37 0.30733046712325773 1102 38-39 0.26648644852221537 1102 40-41 0.25435216447095854 1102 42-43 0.21543591944243445 1102 44-45 0.2020628428857023 1102 46-47 0.23839451105960308 1102 48-49 0.31641502531292787 1102 50-51 0.25131131168998877 1102 52-53 0.10128139390302238 1102 54-55 0.2348898230229608 1102 56-57 0.15936541295874207 1102 58-59 0.02337036020264094 1102 60-61 0.28801248474505314 1102 62-63 0.09551370741019838 1102 64-65 0.18076446814320946 1102 66-67 0.10271147056590024 1102 68-69 0.026995957904752288 1102 70-71 0.1198985093008389 1102 72-73 0.14159410862946586 1102 74-75 0.1012873867547377 1102 76-77 -0.1395200908505494 1102 78-79 -0.0631802362365228 1102 80-81 0.2458780183774607 1102 82-83 0.1405418248934005 1102 84-85 0.17552434522670168 1102 86-87 0.2837722662955855 1102 88-89 0.36568338449907856 1102 90-91 0.351096380479202 1102 92-93 0.49343851443395437 1102 94-95 0.7012607935662913 1102 96-97 0.9689253364703596 1102 98-99 0.8821110138433461 1102 100-101 1.489670608133185 1103 1 0.21696537795905968 1103 2 0.153079176951735 1103 3 0.22010453287438736 1103 4 0.12558039477690386 1103 5 0.06231474772789625 1103 6 0.1156764585261385 1103 7 0.1605258658541544 1103 8 0.14746680133310974 1103 9 0.19393181665240888 1103 10-11 0.11220909147780844 1103 12-13 0.13048955973054177 1103 14-15 0.10407335706137388 1103 16-17 0.3133681295517974 1103 18-19 0.11567955911471728 1103 20-21 0.10239527278346827 1103 22-23 0.14231325888419377 1103 24-25 0.12463001217083303 1103 26-27 0.1859269429202257 1103 28-29 0.3173968215334 1103 30-31 0.2086715945286315 1103 32-33 0.26452498339943276 1103 34-35 0.3834144840731071 1103 36-37 0.500254995425145 1103 38-39 0.5339432761525345 1103 40-41 0.6109972449527774 1103 42-43 0.41702379209282725 1103 44-45 0.2523244532328519 1103 46-47 0.3330605914348297 1103 48-49 0.3949030152216011 1103 50-51 0.4456642591369544 1103 52-53 0.4955930344530657 1103 54-55 0.3886336491964286 1103 56-57 0.47770789599182706 1103 58-59 0.5711939753111963 1103 60-61 0.3244953579284342 1103 62-63 0.5099995475835968 1103 64-65 0.4562934629198949 1103 66-67 0.6224535023322417 1103 68-69 0.5280983634762961 1103 70-71 0.39941250922976934 1103 72-73 0.2569527235603566 1103 74-75 0.24313837989690157 1103 76-77 0.17045930011700605 1103 78-79 0.116154767139097 1103 80-81 0.4414741701736453 1103 82-83 0.5038501964245121 1103 84-85 0.49132312289788516 1103 86-87 0.3131503532088509 1103 88-89 0.2323118533900974 1103 90-91 0.3001210047196885 1103 92-93 0.5208289845330896 1103 94-95 0.7145629468548513 1103 96-97 0.8591520358272184 1103 98-99 1.026036235035118 1103 100-101 1.2415693929109288 1104 1 0.18381736476332478 1104 2 0.28861362769105625 1104 3 0.24285163292113765 1104 4 0.11664806862390975 1104 5 0.03487195666819076 1104 6 0.2169691285204678 1104 7 0.24356339220252465 1104 8 0.13905425963934448 1104 9 0.26866911654192194 1104 10-11 0.23672489527615426 1104 12-13 0.12045824206388289 1104 14-15 0.289995230061578 1104 16-17 0.270740579930667 1104 18-19 0.22826513418178251 1104 20-21 0.23919294341773423 1104 22-23 0.13151042697308668 1104 24-25 0.216826780626441 1104 26-27 0.18683003576835944 1104 28-29 0.21253389835963077 1104 30-31 0.03231414737555127 1104 32-33 0.19226090416234598 1104 34-35 0.1001081194560669 1104 36-37 0.32994755205944415 1104 38-39 0.32431897061329806 1104 40-41 0.27869433860017523 1104 42-43 0.41821257049486604 1104 44-45 0.33070285631917073 1104 46-47 0.2598395472165791 1104 48-49 0.27162365850058023 1104 50-51 0.20105049294593158 1104 52-53 0.24122253522490666 1104 54-55 0.37286152113616566 1104 56-57 0.4918730019991244 1104 58-59 0.40401372764304 1104 60-61 0.5287722828940176 1104 62-63 0.23991046593042853 1104 64-65 0.27988769409599 1104 66-67 0.15109094875395357 1104 68-69 0.2532078055307707 1104 70-71 0.14267620711746076 1104 72-73 0.16031036138812382 1104 74-75 0.4230089431186279 1104 76-77 0.3967186673381953 1104 78-79 0.27980687984090125 1104 80-81 0.49873143213717697 1104 82-83 0.35825798169867795 1104 84-85 0.4053017333755875 1104 86-87 0.3403094177013024 1104 88-89 0.4396444710403067 1104 90-91 0.438280497577221 1104 92-93 0.4447360658780539 1104 94-95 0.4740777450400486 1104 96-97 0.7149014004625478 1104 98-99 0.865869486091011 1104 100-101 1.2209951355627702 1105 1 0.11406424669367965 1105 2 0.19475146161078527 1105 3 0.11469226917154884 1105 4 0.11024415683135658 1105 5 -0.03975337847116123 1105 6 -0.02609611280474411 1105 7 0.16436460700086286 1105 8 -0.016500805543138597 1105 9 -0.06349186457345013 1105 10-11 0.016565304801133607 1105 12-13 -0.08495229647176217 1105 14-15 0.09317786670097661 1105 16-17 0.19260640909781301 1105 18-19 0.16856095029707063 1105 20-21 0.10362649436854099 1105 22-23 0.16939693921117538 1105 24-25 0.06362885949098285 1105 26-27 0.16166448617876483 1105 28-29 0.16698058337635047 1105 30-31 0.21288916461252683 1105 32-33 0.28990211592324755 1105 34-35 0.163746465563257 1105 36-37 0.10969580029178161 1105 38-39 0.26278162073506905 1105 40-41 0.1493264936751757 1105 42-43 0.13845123362825262 1105 44-45 0.14103643044010994 1105 46-47 0.1792815992894461 1105 48-49 0.20996256153456727 1105 50-51 0.2991817172135143 1105 52-53 0.3481584080561646 1105 54-55 0.30313760910996024 1105 56-57 0.21770775939749853 1105 58-59 0.2743431134010095 1105 60-61 0.24827495373260433 1105 62-63 0.4138050874969039 1105 64-65 0.34077861687931943 1105 66-67 0.5895323974515918 1105 68-69 0.5514818798019618 1105 70-71 0.5764019615041533 1105 72-73 0.5207902794208259 1105 74-75 0.31255969143154516 1105 76-77 0.22480324198246038 1105 78-79 0.5092259759636164 1105 80-81 0.5377692033396286 1105 82-83 0.3081060129192892 1105 84-85 0.35373705745774586 1105 86-87 0.4279912604771674 1105 88-89 0.5574499678772895 1105 90-91 0.6467656752938318 1105 92-93 0.6303144028686312 1105 94-95 0.6316321261520876 1105 96-97 0.6332100417087041 1105 98-99 0.8412069294718485 1105 100-101 0.8137886849645781 1106 1 0.08662930880263531 1106 2 0.19868162462304895 1106 3 0.14791954374752692 1106 4 0.033162725784521285 1106 5 -0.004043801584373341 1106 6 -0.0030863234875795342 1106 7 0.03775378096269577 1106 8 0.11355905307200231 1106 9 0.1400207228088206 1106 10-11 0.17993825038757905 1106 12-13 0.1615481667810883 1106 14-15 0.22493184971458646 1106 16-17 0.11312692384704093 1106 18-19 0.17841462675484365 1106 20-21 0.17915548085711208 1106 22-23 0.17670328365230148 1106 24-25 0.048672474087844364 1106 26-27 0.06668390793730339 1106 28-29 0.18423926618289244 1106 30-31 0.17532031916020685 1106 32-33 0.11092025317748977 1106 34-35 0.1596402083647419 1106 36-37 0.16059405941750526 1106 38-39 0.26218417427954677 1106 40-41 0.3364476844651847 1106 42-43 0.2402104324265153 1106 44-45 0.2886617958690678 1106 46-47 0.08738691523324604 1106 48-49 0.17930653024165366 1106 50-51 0.2324541997258862 1106 52-53 0.03866605988488914 1106 54-55 -0.022792729102611986 1106 56-57 0.04354099999087424 1106 58-59 0.07518371732361828 1106 60-61 -0.0683306609902985 1106 62-63 -0.023703823360555987 1106 64-65 0.08050023610559265 1106 66-67 0.14005221050570782 1106 68-69 0.10532938134278425 1106 70-71 0.15752015064808234 1106 72-73 0.11669389958655785 1106 74-75 0.20019148832075828 1106 76-77 -0.07854849185251567 1106 78-79 0.07691094972156165 1106 80-81 0.17345658967731126 1106 82-83 0.36889169243949027 1106 84-85 0.259493817753782 1106 86-87 0.29136831790755835 1106 88-89 0.31641278205903944 1106 90-91 0.46989582543423936 1106 92-93 0.3828115659197806 1106 94-95 0.5656831773762754 1106 96-97 0.4321680244738211 1106 98-99 0.6587196080297879 1106 100-101 0.6817839688583511 1107 1 0.016979998300133303 1107 2 0.04171656116385947 1107 3 -0.0456654195398869 1107 4 0.03255673419160132 1107 5 0.0026750695146375847 1107 6 0.04278013414950266 1107 7 -0.0590768785927267 1107 8 -0.055858630053975844 1107 9 0.04845420143890067 1107 10-11 -0.019229515273309516 1107 12-13 0.028187204115113218 1107 14-15 0.0850790972857638 1107 16-17 0.07441562477408326 1107 18-19 -0.007852314969333918 1107 20-21 -0.035997133689861016 1107 22-23 -0.03683813474759745 1107 24-25 0.013835239987074033 1107 26-27 -0.07843232063230943 1107 28-29 -0.0434584336089614 1107 30-31 -0.14755910749141776 1107 32-33 0.04796509743809452 1107 34-35 -0.09258161368823892 1107 36-37 0.0048760675138694864 1107 38-39 -0.003910353347144735 1107 40-41 0.06888805189117875 1107 42-43 -0.1536033335133098 1107 44-45 0.0358485472068395 1107 46-47 0.11121258032336812 1107 48-49 0.023574635399064903 1107 50-51 0.030129363515747798 1107 52-53 -0.05227813945588622 1107 54-55 0.012186844611214553 1107 56-57 -0.3301199042386145 1107 58-59 0.008976156596467888 1107 60-61 0.011388570261040343 1107 62-63 0.03846526462430688 1107 64-65 -0.032054647401025704 1107 66-67 -0.009463746537299755 1107 68-69 0.03704984134910205 1107 70-71 -0.1316887853666202 1107 72-73 0.09826632639530786 1107 74-75 -0.10471695530922531 1107 76-77 -0.3914339122156001 1107 78-79 -0.09576664156557868 1107 80-81 0.06492075893478244 1107 82-83 0.13815586946494918 1107 84-85 0.21148674888171115 1107 86-87 0.13099482759018244 1107 88-89 0.1870362251505604 1107 90-91 0.13622175975587325 1107 92-93 0.10455262875753135 1107 94-95 0.31877073323078164 1107 96-97 0.26409936778902576 1107 98-99 0.46583045373452947 1107 100-101 0.6635907594950972 1108 1 0.02974391605079063 1108 2 0.03776204505391689 1108 3 0.04961852580031234 1108 4 -0.005590182782768238 1108 5 0.021104381904414993 1108 6 0.06414966713199277 1108 7 0.0796590539630273 1108 8 0.13733640524102952 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64.0 6 125.0 7 332.0 8 826.0 9 1516.0 10 2573.0 11 4173.0 12 5882.0 13 7950.0 14 9939.0 15 12969.0 16 16301.0 17 20342.0 18 18512.0 19 12955.0 20 11962.0 21 12394.0 22 13122.0 23 14910.0 24 17374.0 25 20986.0 26 25741.0 27 31747.0 28 39704.0 29 48933.0 30 60157.0 31 74399.0 32 92583.0 33 116712.0 34 152554.0 35 204710.0 36 288088.0 37 369922.0 38 221130.0 39 12038.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.42765908495494 19.000070513985186 14.723577457553926 22.848692943505945 2 15.96375251604299 20.55226371835137 39.28844863567698 24.195535129928665 3 19.833626007358614 24.50785229060087 29.707862786733507 25.950658915307013 4 13.258357248917237 16.798978412691227 36.43474467005755 33.50791966833398 5 13.795764647774277 37.26868144306559 33.749768475643634 15.185785433516493 6 32.023856134666154 35.82443555967609 17.323428948921386 14.828279356736369 7 29.012504791230953 32.58623572599046 20.190826046803572 18.210433435975006 8 26.907038543362894 34.06345261890613 19.39541739660588 19.634091441125097 9 26.549325485434395 16.732900464082483 20.195923071382264 36.52185097910085 10-11 25.296253564496652 26.438178733540934 26.750761133384525 21.514806568577892 12-13 26.443618556295267 23.722397995710605 27.479900382351286 22.354083065642843 14-15 23.105650172718768 25.08666808669587 26.213095962074927 25.59458577851043 16-17 22.287013200500823 28.318962667482335 26.90956809282566 22.48445603919118 18-19 22.12061811381135 26.587048627457033 29.57204339247449 21.72028986625713 20-21 22.85202363588188 25.639401025387738 29.662980312864168 21.845595025866217 22-23 22.470146640903817 25.654144792811167 29.604802982879157 22.270905583405856 24-25 22.469195700234764 25.892358722624188 29.696790713922994 21.94165486321805 26-27 22.271609956381983 25.94710012850015 29.752428189768608 22.02886172534926 28-29 22.039212276301683 26.25317749818052 29.712235268512583 21.99537495700521 30-31 22.14768260184721 25.94240326563456 29.597514347476338 22.31239978504189 32-33 22.05583415524099 25.970995791935685 29.637244173202053 22.335925879621268 34-35 22.738282645882123 25.963528086378385 29.23433576254743 22.063853505192064 36-37 22.77819556319604 25.79849684722801 29.230257452152706 22.193050137423246 38-39 22.376549403392865 25.966148526619637 29.47529958991299 22.182002480074505 40-41 22.51559458002165 25.971818623355137 29.31896807201393 22.193618724609287 42-43 22.410693705856204 26.12425192215874 28.870376734882036 22.594677637103015 44-45 22.590901981445047 26.11273636161578 28.750890086189155 22.54547157075002 46-47 22.86380556220111 26.520858897283727 28.170791627114895 22.44454391340027 48-49 22.98704830024215 26.570779790234273 27.942249727252076 22.4999221822715 50-51 22.794401945422493 26.818219119337716 27.956110173723058 22.431268761516733 52-53 23.1233553465114 26.838635455648603 27.649884223082992 22.388124974757005 54-55 22.701016511036944 26.705705725791557 27.819561444714445 22.773716318457055 56-57 22.831125993920136 27.037714749647503 27.5419517968831 22.58920745954926 58-59 23.136901814252912 26.637425960344974 27.522158838775233 22.703513386626877 60-61 22.88510511093006 26.70394134152299 28.053403252075604 22.35755029547134 62-63 22.769954259429813 26.693481582860407 28.04290558096698 22.4936585767428 64-65 22.620089560671104 26.831720510607994 27.864709154216104 22.683480774504794 66-67 22.570838797840917 26.992575129797597 27.517142132421075 22.919443939940408 68-69 22.655609473492756 26.79032605359717 27.797739391224958 22.756325081685116 70-71 22.71822696746796 26.77105944322366 27.634510892633614 22.876202696674767 72-73 22.765598390640193 26.712208599373337 27.646465633891225 22.875727376095245 74-75 22.645482418521564 27.223494521111103 27.332959104237553 22.798063956129784 76-77 22.775933274782584 26.96514172317226 27.38793679605971 22.87098820598545 78-79 22.77744220243528 26.954471973056098 27.499364579227965 22.768721245280652 80-81 22.899921278883703 27.18229855369243 27.158862299788535 22.758917867635333 82-83 22.877486816172983 27.178093764864684 27.22599519596496 22.71842422299737 84-85 22.74462024168555 27.135174989587398 27.16892751105768 22.95127725766937 86-87 22.772862039913306 27.04779177680449 27.249969450822253 22.92937673245995 88-89 22.88054902882911 27.07590849416608 26.979536193510228 23.064006283494585 90-91 22.736089224495572 27.007081615457345 27.238169971634946 23.018659188412137 92-93 22.741478781879405 26.89756067694861 27.40174523643475 22.959215304737235 94-95 22.55621360836751 26.828874884043273 27.39002054421328 23.224890963375938 96-97 22.58781745945996 26.703741548048654 27.43456329741093 23.273877695080454 98-99 22.416492954931165 26.81939407597319 27.10000830935069 23.664104659744954 100-101 22.352458134965246 26.876176638109207 27.149771528329097 23.621593698596445 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 287.0 1 229.0 2 304.5 3 399.5 4 311.5 5 275.0 6 399.5 7 825.0 8 1325.0 9 1677.5 10 1727.0 11 1636.0 12 1759.0 13 1934.0 14 2096.5 15 2327.0 16 2530.5 17 2662.5 18 2927.0 19 3203.5 20 3459.5 21 3958.0 22 4670.5 23 5481.5 24 6459.0 25 7807.0 26 9764.5 27 12255.5 28 14629.0 29 17477.5 30 21413.0 31 25710.0 32 30527.0 33 34157.0 34 36811.0 35 39567.0 36 43360.0 37 47325.0 38 49930.5 39 51707.0 40 53144.0 41 56510.5 42 59794.0 43 62552.0 44 65730.0 45 68484.0 46 70308.0 47 73450.0 48 82899.5 49 82808.0 50 73733.5 51 69710.5 52 67190.5 53 67105.0 54 65999.0 55 61575.5 56 56179.5 57 51077.0 58 47695.0 59 43110.0 60 36301.5 61 29732.0 62 24420.0 63 19353.5 64 14211.5 65 10600.5 66 8088.5 67 6535.5 68 5007.0 69 3508.5 70 2605.5 71 2022.0 72 1664.5 73 1449.5 74 1160.0 75 899.0 76 662.5 77 401.0 78 248.5 79 176.5 80 147.5 81 97.0 82 52.0 83 33.0 84 23.0 85 12.5 86 6.0 87 4.5 88 3.5 89 1.5 90 2.0 91 2.5 92 2.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0400276798094312 2 0.006534081408480415 3 0.004218855712562157 4 6.688429788208298E-4 5 7.71741898639419E-4 6 2.0579783963717838E-4 7 0.0 8 0.0 9 0.001800731096825311 10-11 3.858709493197095E-4 12-13 0.0012090623078684231 14-15 0.0016978321770067218 16-17 0.002803995565056556 18-19 0.0 20-21 0.0 22-23 0.0015177590673241907 24-25 7.202924387301245E-4 26-27 0.003987333142970332 28-29 0.00542791802043058 30-31 0.0010547139281405393 32-33 3.086967594557676E-4 34-35 0.008206188855532488 36-37 0.010624313471269336 38-39 0.00848916088503361 40-41 0.0015949332571881325 42-43 3.6014621936506224E-4 44-45 0.0011833375779137759 46-47 0.0 48-49 5.402193290475933E-4 50-51 7.974666285940663E-4 52-53 0.002392399885782199 54-55 0.0023666751558275518 56-57 0.0013119612276870124 58-59 9.003655484126555E-4 60-61 0.0033699396240587966 62-63 0.004373204092290041 64-65 0.008643509264761493 66-67 0.0026753719152833194 68-69 0.0032670407042402075 70-71 1.0289891981858919E-4 72-73 0.0012862364977323652 74-75 0.005350743830566639 76-77 0.002160877316190373 78-79 0.0031384170544669705 80-81 0.004373204092290041 82-83 0.004141681522698216 84-85 0.004964872881246929 86-87 0.0034728385438773856 88-89 0.007743143716348838 90-91 0.01247649402800394 92-93 0.0011061633880498339 94-95 0.002160877316190373 96-97 0.0010804386580951866 98-99 0.003164141784421618 100-101 0.0045275524720179245 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1943655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.41120018665601 #Duplication Level Percentage of deduplicated Percentage of total 1 79.70295114359843 46.555450347159905 2 12.157291261419166 14.202439471964773 3 3.4538539391545475 6.0523126156628 4 1.469545185216654 3.4335159198810583 5 0.8194276387463117 2.393187592264483 6 0.48495443598737903 1.699606238511939 7 0.3200387756830436 1.3085694295740191 8 0.23381676620101408 1.092601435005118 9 0.17711380455389608 0.9310886904256099 >10 1.05169643170717 11.589790924370604 >50 0.08459327484523739 3.4257758551338666 >100 0.04038826383630701 4.31996513053386 >500 0.0030921993219931738 1.1895572188217067 >1k 0.001060182624683373 1.078295869475491 >5k 1.766971041138955E-4 0.727843261214756 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7360 0.37866802493240825 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6744 0.3469751576282828 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3747 0.1927811262801269 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3521 0.1811535483406263 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2070 0.10650038201223982 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.14494599092946E-5 0.0 0.0 0.0 0.0 3 5.14494599092946E-5 0.0 0.0 0.0 0.0 4 5.14494599092946E-5 0.0 0.0 0.0 0.0 5 5.14494599092946E-5 0.0 0.0 0.0 0.0 6 5.14494599092946E-5 0.0 0.0 0.0 0.0 7 5.14494599092946E-5 0.0 0.0 0.0 0.0 8 5.14494599092946E-5 0.0 0.0 0.0 0.0 9 5.14494599092946E-5 0.0 0.0 0.0 0.0 10-11 5.14494599092946E-5 0.0 0.0 0.0 0.0 12-13 1.028989198185892E-4 0.0 0.0 0.0 0.0 14-15 1.8007310968253112E-4 0.0 0.0 2.57247299546473E-5 5.14494599092946E-5 16-17 2.057978396371784E-4 0.0 0.0 5.14494599092946E-5 5.14494599092946E-5 18-19 2.315225695918257E-4 0.0 0.0 7.71741898639419E-5 5.14494599092946E-5 20-21 2.57247299546473E-4 0.0 0.0 1.028989198185892E-4 5.14494599092946E-5 22-23 2.57247299546473E-4 0.0 0.0 2.057978396371784E-4 5.14494599092946E-5 24-25 3.6014621936506224E-4 0.0 0.0 2.057978396371784E-4 7.71741898639419E-5 26-27 3.6014621936506224E-4 0.0 0.0 2.057978396371784E-4 1.028989198185892E-4 28-29 5.402193290475933E-4 0.0 0.0 4.630451391836514E-4 1.028989198185892E-4 30-31 6.173935189115352E-4 0.0 0.0 0.001440584877460249 1.028989198185892E-4 32-33 6.431182488661825E-4 0.0 0.0 0.00257247299546473 1.028989198185892E-4 34-35 7.202924387301245E-4 0.0 0.0 0.003935883683061037 1.028989198185892E-4 36-37 7.71741898639419E-4 0.0 0.0 0.005325019100611991 1.028989198185892E-4 38-39 8.231913585487136E-4 0.0 0.0 0.00686850289789083 1.028989198185892E-4 40-41 8.231913585487136E-4 0.0 0.0 0.008746408184580081 1.028989198185892E-4 42-43 8.231913585487136E-4 0.0 0.0 0.012064898348729584 1.028989198185892E-4 44-45 8.746408184580083E-4 0.0 0.0 0.0176214400189334 1.028989198185892E-4 46-47 9.775397382765973E-4 0.0 0.0 0.0290946695787061 1.028989198185892E-4 48-49 9.775397382765973E-4 0.0 0.0 0.053455988845757085 1.028989198185892E-4 50-51 9.775397382765973E-4 0.0 0.0 0.0704857600757336 1.543483797278838E-4 52-53 0.0010032644682312448 2.57247299546473E-5 0.0 0.08787567752507518 1.543483797278838E-4 54-55 0.001028989198185892 5.14494599092946E-5 0.0 0.11318881180044812 1.543483797278838E-4 56-57 0.001028989198185892 5.14494599092946E-5 0.0 0.13119612276870124 1.543483797278838E-4 58-59 0.001028989198185892 5.14494599092946E-5 0.0 0.15283062066055964 1.543483797278838E-4 60-61 0.001028989198185892 5.14494599092946E-5 0.0 0.18992568125516102 1.543483797278838E-4 62-63 0.001028989198185892 5.14494599092946E-5 0.0 0.21773411433613477 1.543483797278838E-4 64-65 0.0010804386580951866 5.14494599092946E-5 0.0 0.24760052581348027 1.543483797278838E-4 66-67 0.0010804386580951866 5.14494599092946E-5 0.0 0.30648443267966796 1.543483797278838E-4 68-69 0.0010804386580951866 5.14494599092946E-5 0.0 0.37398612408066245 1.543483797278838E-4 70-71 0.0011318881180044814 7.71741898639419E-5 0.0 0.42983451281220175 1.543483797278838E-4 72-73 0.0011318881180044814 1.028989198185892E-4 0.0 0.4900561056360311 1.543483797278838E-4 74-75 0.0011318881180044814 1.028989198185892E-4 0.0 0.5399363570180922 1.543483797278838E-4 76-77 0.0011318881180044814 1.028989198185892E-4 0.0 0.5812245485953011 1.543483797278838E-4 78-79 0.0011318881180044814 1.028989198185892E-4 0.0 0.6404428769508992 1.543483797278838E-4 80-81 0.0013376859576416597 1.028989198185892E-4 0.0 0.7418497624321189 1.543483797278838E-4 82-83 0.001440584877460249 1.028989198185892E-4 0.0 0.8412243942469214 1.543483797278838E-4 84-85 0.0014920343373695435 1.028989198185892E-4 0.0 0.9618991024641719 1.8007310968253112E-4 86-87 0.0015692085272334855 1.028989198185892E-4 0.0 1.182154240335862 2.057978396371784E-4 88-89 0.0015949332571881328 1.028989198185892E-4 0.0 1.4488939652355999 2.057978396371784E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1850 0.0 32.354076 2 GTACCTG 2020 0.0 30.351551 1 GTACATG 8185 0.0 23.400703 1 TACATGG 8325 0.0 22.93887 2 ACATGGG 8275 0.0 21.984482 3 CGTATAG 130 2.931138E-4 21.935646 1 GTATCAA 14635 0.0 21.368582 1 GAGTACT 8250 0.0 20.985971 12-13 AGTACTT 8570 0.0 20.063803 12-13 GGTATCA 11455 0.0 19.541971 1 GTACTTT 8845 0.0 19.439999 14-15 TATCACG 125 0.0060296766 19.0016 2 CATGGGG 4090 0.0 18.464474 4 TCAACGC 17330 0.0 18.06134 4 ATCAACG 17320 0.0 18.045275 3 TATAGGG 370 1.4188117E-10 17.974485 2 CAACGCA 17525 0.0 17.887476 5 TATCAAC 17540 0.0 17.82077 2 AACGCAG 17750 0.0 17.71425 6 ACCTGGG 3345 0.0 17.466042 3 >>END_MODULE