FastQCFastQC Report
Thu 26 May 2016
SRR936440_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936440_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008330
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61770.30756897521821613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52760.2627058302171456No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT30990.15430731005362663No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.14021600035850681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTG21350.031.8380621
TACCTGG21000.031.2338092
GTATCAA133750.025.233241
GTACATG89100.025.0209391
TACATGG89950.024.4121482
GTGTACG1852.525885E-723.1248661
ACATGGG92150.023.0579473
GAGTACT71200.022.0661712-13
GGTATCA98450.021.630711
ATCAACG153300.021.5501883
TATCAAC154000.021.4809592
TCAACGC154300.021.4732074
CAACGCA155000.021.3471585
AACGCAG157850.021.0526126
GGGCGTA3700.020.5646639
GTACTTT76950.020.3836214-15
AGTACTT75100.019.90747612-13
CATGGGG44950.019.5646514
ACGCAGA171200.019.3554127
CGCAGAG172400.019.1703138
Produced by FastQC (version 0.11.5)