FastQCFastQC Report
Thu 26 May 2016
SRR936440_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936440_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2008330
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.3773782197148875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67580.3364984838149109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.1989214919858788No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35890.17870569079782705No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.10028232412004003No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG20450.031.1264152
GTACCTG21950.030.5187761
GTACATG89000.023.7548641
TATCACG1201.7107945E-423.7513142
TACATGG91050.023.0078622
GGTATCA116750.022.5849231
ACATGGG90200.022.22133
GTATCAA150200.021.9835281
GAGTACT84600.021.69752712-13
GTACTTT89500.020.42951214-15
CATGGGG42800.019.6398874
AGTACTT88100.019.24524512-13
ATCAACG174050.018.9659393
CAACGCA175300.018.8554485
TCAACGC175050.018.8552444
TATCAAC176300.018.6992742
AACGCAG178450.018.6285976
ATACCGA1300.007574588518.2652386
CATGGGA48850.017.888044
ACTTTTT106000.017.6526816-17
Produced by FastQC (version 0.11.5)