##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936440_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2008330 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.876070665677453 31.0 31.0 34.0 30.0 34.0 2 32.03280934906116 31.0 31.0 34.0 30.0 34.0 3 32.11518575134564 33.0 31.0 34.0 30.0 34.0 4 35.816789073508836 37.0 35.0 37.0 35.0 37.0 5 35.646384309351554 37.0 35.0 37.0 33.0 37.0 6 35.73192254261003 37.0 35.0 37.0 33.0 37.0 7 35.675134564538695 37.0 35.0 37.0 33.0 37.0 8 35.64589932929349 37.0 35.0 37.0 33.0 37.0 9 37.27421838044545 39.0 37.0 39.0 34.0 39.0 10-11 37.162768568910494 39.0 37.0 39.0 33.0 39.0 12-13 37.05545179328098 39.0 37.0 39.0 33.0 39.0 14-15 38.1844642065796 40.0 37.5 41.0 33.0 41.0 16-17 38.07816842849532 40.0 37.0 41.0 33.0 41.0 18-19 38.22619763684255 40.0 37.5 41.0 33.5 41.0 20-21 38.24679659219351 40.0 38.0 41.0 33.0 41.0 22-23 38.16219943933517 40.0 37.5 41.0 33.0 41.0 24-25 38.02820278539882 40.0 37.0 41.0 33.0 41.0 26-27 37.83198702404485 40.0 37.0 41.0 33.0 41.0 28-29 37.74422380784034 40.0 37.0 41.0 32.5 41.0 30-31 37.71213645167876 40.0 37.0 41.0 33.0 41.0 32-33 37.55149776182201 40.0 36.5 41.0 32.0 41.0 34-35 37.37285630349594 39.5 36.0 41.0 32.0 41.0 36-37 37.17024542779324 39.0 36.0 41.0 31.0 41.0 38-39 37.00464714464256 39.0 36.0 41.0 31.0 41.0 40-41 36.815786250267635 39.0 35.5 41.0 31.0 41.0 42-43 36.53565898034685 39.0 35.0 41.0 30.0 41.0 44-45 36.26947115264922 39.0 35.0 40.0 30.0 41.0 46-47 35.8953339341642 39.0 35.0 40.0 29.0 41.0 48-49 35.50987711182923 38.0 35.0 40.0 27.5 41.0 50-51 35.21311238690853 38.0 34.0 40.0 27.0 41.0 52-53 34.8441834758232 38.0 34.0 40.0 26.0 41.0 54-55 34.66002400003984 38.0 34.0 40.0 26.0 41.0 56-57 34.35340979819053 38.0 33.5 40.0 25.0 41.0 58-59 33.976897472029 37.0 33.0 40.0 24.0 41.0 60-61 33.54934746779663 37.0 32.5 39.5 23.0 41.0 62-63 33.222192567954465 36.0 32.0 39.0 22.5 40.5 64-65 32.793335507610806 36.0 31.5 39.0 22.0 40.0 66-67 32.36954086230848 35.0 31.0 38.5 20.5 40.0 68-69 32.01583479806605 35.0 31.0 38.0 20.0 40.0 70-71 31.641857911797363 35.0 31.0 37.5 18.5 39.5 72-73 31.184002380086937 34.5 30.0 37.0 18.0 39.0 74-75 30.689107865739196 34.0 30.0 36.0 14.5 39.0 76-77 29.198193523972655 32.5 28.0 35.0 12.5 37.0 78-79 29.964193135590264 34.0 29.0 35.0 11.0 37.0 80-81 29.74440156747148 34.0 29.0 35.0 7.0 37.0 82-83 29.452738095830863 34.0 29.0 35.0 7.0 36.5 84-85 29.077686435994085 34.0 29.0 35.0 2.0 36.0 86-87 28.700623403524318 33.5 29.0 35.0 2.0 36.0 88-89 28.231897397340077 33.0 27.5 35.0 2.0 35.0 90-91 27.763921018956047 33.0 27.0 35.0 2.0 35.0 92-93 27.149657675780375 33.0 25.5 34.5 2.0 35.0 94-95 26.60895644640074 32.0 25.0 34.0 2.0 35.0 96-97 25.88483341881065 32.0 24.5 34.0 2.0 35.0 98-99 24.988161557114616 32.0 20.0 34.0 2.0 35.0 100-101 21.725506515363513 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3834515112003878 1101 2 0.41360753071977996 1101 3 0.4056022800852972 1101 4 0.3325061718016542 1101 5 0.3323664053902817 1101 6 0.320787248798581 1101 7 0.3407480254171489 1101 8 0.336782476382929 1101 9 0.31766975093746197 1101 10-11 0.4110188060565747 1101 12-13 0.4180409242269505 1101 14-15 0.5999579082352042 1101 16-17 0.6134574611681884 1101 18-19 0.4762524767903784 1101 20-21 0.41906835910794626 1101 22-23 0.3680826069290646 1101 24-25 0.3852717340001419 1101 26-27 0.43898170780575896 1101 28-29 0.440644796164257 1101 30-31 0.5258969884806604 1101 32-33 0.5231056330764474 1101 34-35 0.44856610092663374 1101 36-37 0.45083074685332747 1101 38-39 0.39420299078715715 1101 40-41 0.4264112118738268 1101 42-43 0.5666675722205738 1101 44-45 0.675933500180804 1101 46-47 0.62767267216298 1101 48-49 0.4398169080957288 1101 50-51 0.4745944933004793 1101 52-53 0.3798928950860301 1101 54-55 0.36331110496655583 1101 56-57 0.42310974509712196 1101 58-59 0.1483906312008969 1101 60-61 0.19733617540499893 1101 62-63 0.27960534581222163 1101 64-65 0.33483605538106076 1101 66-67 0.20132998330220886 1101 68-69 0.2690556100961743 1101 70-71 0.2475990287572074 1101 72-73 0.3133002390448034 1101 74-75 0.34055043212017466 1101 76-77 0.3731998300043493 1101 78-79 0.2854504387967296 1101 80-81 0.5242607498678034 1101 82-83 0.4967686114898626 1101 84-85 0.5065999026196017 1101 86-87 0.5103337428749164 1101 88-89 0.7109670165760171 1101 90-91 0.7636020423815104 1101 92-93 0.5954699500767617 1101 94-95 0.329034727317552 1101 96-97 0.5695919240556222 1101 98-99 0.6251741271379814 1101 100-101 1.411456867962741 1102 1 0.2443694923834805 1102 2 0.23582879580922622 1102 3 0.2449850170437955 1102 4 0.20870218221266157 1102 5 0.20124521680657637 1102 6 0.20256861566206652 1102 7 0.22740833607325328 1102 8 0.22302521758525984 1102 9 0.34781578344364306 1102 10-11 0.3035612248836017 1102 12-13 0.3365350656009767 1102 14-15 0.35114576679499265 1102 16-17 0.37415220329715027 1102 18-19 0.34111303390005077 1102 20-21 0.30023456537752224 1102 22-23 0.25066735698113973 1102 24-25 0.2288446441574905 1102 26-27 0.31788607953406967 1102 28-29 0.24304319246395067 1102 30-31 0.42142712161620466 1102 32-33 0.387152100088457 1102 34-35 0.32006518499922976 1102 36-37 0.3013436807994836 1102 38-39 0.17556523082827624 1102 40-41 0.1965443376877971 1102 42-43 0.18052569496847326 1102 44-45 0.3072657852104044 1102 46-47 0.16029029729268274 1102 48-49 0.2638225769174056 1102 50-51 0.16868191910646857 1102 52-53 0.21682338485898356 1102 54-55 0.23350978626469754 1102 56-57 -0.04382172289798092 1102 58-59 0.20287115630664943 1102 60-61 0.2248542700813303 1102 62-63 0.11776713012835671 1102 64-65 0.0661661260271913 1102 66-67 -0.060090638594004986 1102 68-69 0.003632550918119648 1102 70-71 0.061064353945504024 1102 72-73 0.18321762064098834 1102 74-75 0.12134119088961981 1102 76-77 0.07388283346754676 1102 78-79 0.15567187227665258 1102 80-81 0.2908121712586471 1102 82-83 0.14049657715767339 1102 84-85 0.19578040066607727 1102 86-87 0.26643266595453596 1102 88-89 0.19825596879789487 1102 90-91 0.12020185413458151 1102 92-93 0.3123517769479882 1102 94-95 0.2423407628995271 1102 96-97 0.5925263666247389 1102 98-99 0.468959350435437 1102 100-101 0.899267173654728 1103 1 0.2294806659874773 1103 2 0.3103069237116003 1103 3 0.2253948089795017 1103 4 0.2178854752750894 1103 5 0.3109889106786099 1103 6 0.2169271519225333 1103 7 0.1898888635789575 1103 8 0.13875372013657028 1103 9 0.2517150926094942 1103 10-11 0.21811455546642122 1103 12-13 0.2821701510579331 1103 14-15 0.34280046165499556 1103 16-17 0.24415965927358485 1103 18-19 0.3162163398763056 1103 20-21 0.21544087206051898 1103 22-23 0.2138540514859173 1103 24-25 0.2496395104494482 1103 26-27 0.2918751518107783 1103 28-29 0.19075688574395144 1103 30-31 0.38564514139766715 1103 32-33 0.1909094820601709 1103 34-35 0.2929838482518079 1103 36-37 0.0883512487080651 1103 38-39 0.24352585476214728 1103 40-41 0.29033446212675074 1103 42-43 0.3357917789712346 1103 44-45 0.3850313419294764 1103 46-47 0.44483061691909853 1103 48-49 0.2365965840514619 1103 50-51 0.3047864443106292 1103 52-53 0.3869190914746383 1103 54-55 0.32677852426205334 1103 56-57 0.3227100547326103 1103 58-59 0.2973222354806069 1103 60-61 0.3140175957428397 1103 62-63 0.39028757856001306 1103 64-65 0.3327820377839572 1103 66-67 0.18903078651506178 1103 68-69 0.36129671190800394 1103 70-71 0.13868340442782667 1103 72-73 0.3134918451639379 1103 74-75 0.26992441660774347 1103 76-77 0.4089449095592066 1103 78-79 0.21353108198015036 1103 80-81 0.27069016983775995 1103 82-83 0.4109587690667382 1103 84-85 0.5500734149896935 1103 86-87 0.556730206592384 1103 88-89 0.4472575987520493 1103 90-91 0.4828674628419769 1103 92-93 0.4673649729162115 1103 94-95 0.5928916818532493 1103 96-97 0.7719231957071173 1103 98-99 0.7059705954539943 1103 100-101 1.104091911955507 1104 1 0.17282606907125952 1104 2 0.15355831861477753 1104 3 0.11343221285382299 1104 4 0.16604002984129806 1104 5 0.17499489438092297 1104 6 0.059873273897594004 1104 7 0.09942837789502335 1104 8 0.13436740695124882 1104 9 0.21471354330527248 1104 10-11 0.2308560607619441 1104 12-13 0.1672151523460812 1104 14-15 0.31461995447843094 1104 16-17 0.36081582666902534 1104 18-19 0.14526625729379816 1104 20-21 0.12005190494176787 1104 22-23 0.177668732290428 1104 24-25 0.19995104764863214 1104 26-27 0.19574384651314602 1104 28-29 0.19507096129635926 1104 30-31 0.24314775298110192 1104 32-33 0.1715221145362662 1104 34-35 0.23963188974069283 1104 36-37 0.15558353052615814 1104 38-39 0.21192812154597362 1104 40-41 0.2278618131694401 1104 42-43 0.3354490086458881 1104 44-45 0.24458153197510057 1104 46-47 0.26205903871100134 1104 48-49 0.5192608976461699 1104 50-51 0.45413714379148473 1104 52-53 0.37932300108561634 1104 54-55 0.44089049325479834 1104 56-57 0.3615994877013833 1104 58-59 0.30052517492363506 1104 60-61 0.44578245706423303 1104 62-63 0.5060015687065729 1104 64-65 0.5740773478742014 1104 66-67 0.3530680033435374 1104 68-69 0.2865646197826379 1104 70-71 0.5110770044602972 1104 72-73 0.6262677818930342 1104 74-75 0.570710670855977 1104 76-77 0.375827539176683 1104 78-79 0.3009357503619903 1104 80-81 0.4750180091406797 1104 82-83 0.35250396622363667 1104 84-85 0.6789171187796619 1104 86-87 0.7772672884302345 1104 88-89 0.7903942957342593 1104 90-91 0.8023802481309978 1104 92-93 0.6566724577496181 1104 94-95 0.5423515869892448 1104 96-97 0.8315823267203761 1104 98-99 0.8764399393465929 1104 100-101 1.4139951069102068 1105 1 0.030813982836122022 1105 2 0.07275058140470492 1105 3 0.09698976431188555 1105 4 0.20257878060800039 1105 5 0.2571990074319572 1105 6 0.14112621688750693 1105 7 0.10754520023395742 1105 8 0.22902637098029288 1105 9 0.21423774433632303 1105 10-11 0.21433584561695085 1105 12-13 0.23937903000499006 1105 14-15 0.24797814745213742 1105 16-17 0.3279052569789158 1105 18-19 0.19905646460651383 1105 20-21 0.1608868593932158 1105 22-23 0.22888665142728115 1105 24-25 0.20131715120807314 1105 26-27 0.1210236190880849 1105 28-29 0.08673192115659134 1105 30-31 0.21532998655346347 1105 32-33 0.1617751886704113 1105 34-35 0.2172897693160607 1105 36-37 0.18134663270120654 1105 38-39 0.20241385367415177 1105 40-41 0.2151768714580271 1105 42-43 0.035466530199641966 1105 44-45 0.23094263900355827 1105 46-47 0.13356807236629464 1105 48-49 0.1076878365109124 1105 50-51 0.3184787066926873 1105 52-53 0.5044850861547943 1105 54-55 0.3132798833656665 1105 56-57 0.25376159822922517 1105 58-59 0.30032193345758174 1105 60-61 0.38725608177736603 1105 62-63 0.14759872144339425 1105 64-65 0.3599420322733593 1105 66-67 0.3410148216839701 1105 68-69 0.22020007690370846 1105 70-71 0.342073060934041 1105 72-73 0.4007146816370124 1105 74-75 0.2754811545471334 1105 76-77 0.17505556390710453 1105 78-79 0.2838216422593547 1105 80-81 0.3400988361323414 1105 82-83 0.4337684618863591 1105 84-85 0.6270919355360896 1105 86-87 0.6944042451524233 1105 88-89 0.39776975738655196 1105 90-91 0.5625113538522903 1105 92-93 0.3314079175877147 1105 94-95 0.6938007093391114 1105 96-97 0.572867172202308 1105 98-99 0.2920842479544241 1105 100-101 0.7167217730249149 1106 1 0.202378652233822 1106 2 0.19778722357030887 1106 3 0.12233551589346803 1106 4 0.13908035393263418 1106 5 0.18337343487247182 1106 6 0.050970601582157826 1106 7 0.04998401439352307 1106 8 0.11297387225290123 1106 9 0.08261150978732701 1106 10-11 0.044682040166271975 1106 12-13 -0.013562707215605485 1106 14-15 0.06620386066703787 1106 16-17 0.07575686341564847 1106 18-19 0.2083260644561733 1106 20-21 0.10872945916985799 1106 22-23 0.05589764607610448 1106 24-25 0.03663194976086004 1106 26-27 0.04520605950997947 1106 28-29 -0.11044428046970012 1106 30-31 0.08423710200433021 1106 32-33 0.043343816368953014 1106 34-35 0.06503607358671104 1106 36-37 -0.141657555606173 1106 38-39 -0.010633955908218695 1106 40-41 0.12186766250582792 1106 42-43 0.15342010760083724 1106 44-45 0.08497519218757077 1106 46-47 0.18795335834563787 1106 48-49 -0.048102025483061084 1106 50-51 0.11459275373649547 1106 52-53 -0.014744659223985934 1106 54-55 0.01651464217130183 1106 56-57 -0.06919713590574617 1106 58-59 -0.010340815732817532 1106 60-61 -0.03600065075365677 1106 62-63 0.03221982829879266 1106 64-65 0.06950049759740295 1106 66-67 -0.05832596655451283 1106 68-69 -0.155250755886172 1106 70-71 0.10971974901887904 1106 72-73 0.32093103508024257 1106 74-75 0.11588471604989081 1106 76-77 0.035958641512319645 1106 78-79 -0.18123588357324039 1106 80-81 0.09215579526083317 1106 82-83 0.0907821143416534 1106 84-85 0.3944223383379182 1106 86-87 0.5840915983075625 1106 88-89 0.2928087080348938 1106 90-91 0.30234398148855135 1106 92-93 0.5109880935921645 1106 94-95 0.5439123140628936 1106 96-97 0.9068447552987422 1106 98-99 0.9565980492931772 1106 100-101 1.332839191253175 1107 1 0.07910746836236981 1107 2 0.07742948520439086 1107 3 0.03549786241461561 1107 4 0.11214204864234745 1107 5 0.05567296496190721 1107 6 -0.02593162670342508 1107 7 -0.06146482598847314 1107 8 0.05046788468279573 1107 9 -0.06451900408261935 1107 10-11 -0.02638480840125368 1107 12-13 -0.11941021365792182 1107 14-15 -0.05335199805663393 1107 16-17 0.08513082127527838 1107 18-19 0.003169478129059655 1107 20-21 0.013216953485454042 1107 22-23 -0.009292895078964136 1107 24-25 0.10943170722637774 1107 26-27 0.12983846818058709 1107 28-29 0.08273716564217182 1107 30-31 0.08179993359444637 1107 32-33 0.164408845927845 1107 34-35 0.18007078633493023 1107 36-37 0.16188496372079442 1107 38-39 0.2143167792744478 1107 40-41 0.31927466568909324 1107 42-43 0.2687391920331592 1107 44-45 0.07007205439767006 1107 46-47 0.17943706305990048 1107 48-49 0.07462012094713799 1107 50-51 0.030856206821226806 1107 52-53 0.26991230606326866 1107 54-55 0.095265589571639 1107 56-57 -0.06778315985602745 1107 58-59 0.06612757899206656 1107 60-61 -0.0029134899229816824 1107 62-63 0.10583359213083554 1107 64-65 0.06306363235562884 1107 66-67 0.0016418976261718399 1107 68-69 0.20878653379095624 1107 70-71 0.2531713179138322 1107 72-73 0.4319481640782712 1107 74-75 0.34553637589527497 1107 76-77 0.15816904017837885 1107 78-79 0.34917399970703755 1107 80-81 0.4140733335358071 1107 82-83 0.385105291543411 1107 84-85 0.644694280129638 1107 86-87 0.5070077723260553 1107 88-89 0.3720968693203268 1107 90-91 0.5498316273882047 1107 92-93 0.4565636582427608 1107 94-95 0.5584637768490062 1107 96-97 0.9408623390489339 1107 98-99 0.7763200441393217 1107 100-101 1.529165703304109 1108 1 0.0709668787932074 1108 2 0.05908275257193907 1108 3 -0.05556707265031946 1108 4 0.011522794880235665 1108 5 0.06634499134821681 1108 6 0.01606837329657651 1108 7 -0.07246791813442144 1108 8 0.03207291457353989 1108 9 0.05905551663476416 1108 10-11 0.06517858059070392 1108 12-13 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2316 58-59 -1.853699036137698 2316 60-61 -1.3425596031814635 2316 62-63 -1.2289544316743033 2316 64-65 -1.3910631238870863 2316 66-67 -1.158670759592063 2316 68-69 -1.6241613771238264 2316 70-71 -1.585711435629328 2316 72-73 -0.9689635482967205 2316 74-75 -1.0890978961459545 2316 76-77 -1.1801139219665409 2316 78-79 -1.4821185949306717 2316 80-81 -1.0620436061537468 2316 82-83 -1.5299845912714858 2316 84-85 -1.2227851971389043 2316 86-87 -1.4283088728669 2316 88-89 -1.7067047749523283 2316 90-91 -1.735320345125448 2316 92-93 -1.7344452380085436 2316 94-95 -1.8888723787433577 2316 96-97 -1.8736562203718918 2316 98-99 -2.3151703540446213 2316 100-101 -3.2914994908795876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 11.0 4 28.0 5 77.0 6 160.0 7 376.0 8 827.0 9 1663.0 10 2595.0 11 4160.0 12 6035.0 13 8219.0 14 10277.0 15 13248.0 16 16658.0 17 20536.0 18 18753.0 19 13337.0 20 12543.0 21 12773.0 22 13710.0 23 15475.0 24 18012.0 25 21695.0 26 26720.0 27 33161.0 28 41157.0 29 50623.0 30 62488.0 31 76710.0 32 95910.0 33 121299.0 34 156805.0 35 211523.0 36 296714.0 37 381710.0 38 229192.0 39 13150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46981819449528 18.998911806525676 14.682444226971757 22.84882577200728 2 16.00830254902996 20.557224940713866 39.15574151742629 24.27873099282989 3 19.783830889126808 24.467345566815226 29.716256255757788 26.03256728830018 4 13.184187857573207 16.82960469643933 36.45909785742383 33.52710958856363 5 13.834712636809975 37.163863244798975 33.79537510083356 15.206049017557488 6 32.02541415006498 35.860839603053286 17.28505773453566 14.828688512346078 7 28.937639201545167 32.57130679286113 20.187230279501016 18.303823726092688 8 26.91818575632491 34.08050469793311 19.37186617737125 19.629443368370737 9 26.573122942942646 16.658965409071218 20.11671388666205 36.651197761324084 10-11 25.31675571245761 26.36750434440555 26.796044474762613 21.519695468374223 12-13 26.465948682148692 23.7270994922589 27.444285161581373 22.36266666401103 14-15 23.125172251794968 25.09689046765102 26.162081874716957 25.615855405837056 16-17 22.32495273433785 28.333878562725527 26.822995634679376 22.518173068257244 18-19 22.143396688647083 26.62663452676215 29.4739140458387 21.75605473875207 20-21 22.94575592656585 25.631445031444034 29.547883067025836 21.874915974964274 22-23 22.499816389508183 25.673875401297845 29.482243309420404 22.34406489977357 24-25 22.578535400058755 25.83564454048886 29.629542953598264 21.95627710585412 26-27 22.31613823762489 25.96963444871534 29.565370235605272 22.148857078054498 28-29 22.055979040661626 26.269631640574136 29.63195427705296 22.042435041711283 30-31 22.251744135963683 25.890317238302423 29.491470651062762 22.366467974671128 32-33 22.060975761574564 26.031147683842292 29.528850822263998 22.379025732319146 34-35 22.73800098591339 26.02029587360391 29.151019026136666 22.090684114346036 36-37 22.776061992344317 25.76775277751844 29.21223664145894 22.243948588678304 38-39 22.415575362246678 25.9955932878554 29.39824727381367 22.19058407608425 40-41 22.573540853124936 25.94760054722287 29.257651597290774 22.221207002361425 42-43 22.438759382659125 26.11048258512737 28.795812821863677 22.65494521034983 44-45 22.581473982028033 26.18109545188716 28.669251446936695 22.568179119148112 46-47 22.912668203105724 26.563623838490162 28.060245274229974 22.463462684174136 48-49 23.03172831573111 26.5286123974981 27.877876818718562 22.561782468052222 50-51 22.853244801955857 26.762243707761808 27.883649454583477 22.50086203569886 52-53 23.183268847604634 26.878884921791812 27.536597030710407 22.401249199893144 54-55 22.764550893552247 26.678972237526725 27.766730592895648 22.789746276025376 56-57 22.888912515770738 27.029105800817256 27.448239491591554 22.633742191820456 58-59 23.14336492625455 26.58984957566077 27.49986117243181 22.76692432565287 60-61 22.91713874353763 26.6614657283397 27.981454911450417 22.439940616672253 62-63 22.7797763641597 26.77208545849132 27.949101781497404 22.499036395851572 64-65 22.662700897776038 26.792993586958282 27.762158447324286 22.78214706794139 66-67 22.64532899927525 26.946647615533593 27.52657187408426 22.881451511106896 68-69 22.691984132931637 26.75609672906655 27.731095365980508 22.82082377202131 70-71 22.734253883266607 26.796960756171114 27.517634689743236 22.951150670819043 72-73 22.805670085511366 26.69230820938496 27.58394476603565 22.918076939068015 74-75 22.73860410829953 27.1952080876343 27.205863863500507 22.860323940565667 76-77 22.891787937310642 26.929863238930164 27.31646204333203 22.861886780427167 78-79 22.824993321001983 26.94483334374917 27.363973148560582 22.866200186688264 80-81 22.970466152233403 27.167579250361673 27.08560856757975 22.776346029825177 82-83 22.916947312041884 27.16028048518429 27.16503641272587 22.75773579004796 84-85 22.823058313172602 27.065465199239476 27.1204501393923 22.99102634819562 86-87 22.846820557382845 27.062946465252587 27.098128362883973 22.9921046144806 88-89 22.881788397383453 27.071999880454722 26.9069775840148 23.139234138147025 90-91 22.772078223057306 26.964050837963644 27.128707100891624 23.13516383808743 92-93 22.80772749753336 26.927055654697128 27.233686175503323 23.031530672266186 94-95 22.614478176945756 26.856316212959282 27.2850996389782 23.244105971116763 96-97 22.58858575379929 26.70660129343358 27.38096513109309 23.32384782167404 98-99 22.464085962047875 26.789263212692056 26.99923657372294 23.747414251537126 100-101 22.368540683830815 26.839000620495895 27.086700787713436 23.70575790795986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 272.0 1 226.5 2 319.0 3 425.0 4 330.0 5 293.5 6 437.0 7 838.0 8 1350.0 9 1695.5 10 1736.5 11 1634.5 12 1696.0 13 1839.0 14 2038.5 15 2252.0 16 2491.5 17 2767.5 18 2939.5 19 3189.5 20 3573.0 21 4054.0 22 4665.0 23 5508.5 24 6459.0 25 7807.0 26 9858.0 27 12401.0 28 15095.0 29 18153.5 30 22092.5 31 26597.5 32 31422.0 33 34887.5 34 37714.0 35 40638.0 36 44454.5 37 48745.0 38 51461.5 39 53292.0 40 54873.5 41 58107.0 42 61349.5 43 64093.5 44 67304.0 45 70533.0 46 72606.5 47 75683.0 48 85143.0 49 85339.5 50 76451.5 51 72418.5 52 70035.5 53 69955.5 54 69235.0 55 64599.0 56 58716.0 57 53261.0 58 49517.5 59 44716.0 60 37565.0 61 31021.5 62 25616.5 63 19986.0 64 14706.0 65 11191.0 66 8596.0 67 6986.0 68 5343.5 69 3735.5 70 2718.5 71 2080.0 72 1747.0 73 1528.0 74 1220.5 75 939.5 76 654.5 77 404.5 78 263.5 79 183.5 80 128.5 81 94.0 82 67.5 83 40.5 84 21.5 85 12.0 86 7.5 87 7.0 88 5.5 89 3.5 90 2.5 91 1.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020663934711924833 2 0.013543590943719408 3 0.008962670477461373 4 0.0 5 0.003485482963457201 6 0.0 7 4.9792613763674304E-5 8 0.0 9 0.0 10-11 0.0 12-13 0.0011452301165645088 14-15 2.738593757002086E-4 16-17 4.9792613763674304E-5 18-19 0.001344400571619206 20-21 0.0 22-23 1.2448153440918576E-4 24-25 0.0 26-27 0.0017178451748467633 28-29 0.003012453132702295 30-31 0.0014439857991465545 32-33 0.007717855133369515 34-35 0.001991704550546972 36-37 8.713707408643002E-4 38-39 0.0014937784129102289 40-41 3.734446032275572E-4 42-43 0.005178431831422126 44-45 0.0017676377886104374 46-47 3.236519894638829E-4 48-49 6.72200285809603E-4 50-51 1.2448153440918576E-4 52-53 2.2406676193653435E-4 54-55 0.0012697116509736946 56-57 0.01292118327167348 58-59 0.02208302420418955 60-61 0.017875548341159075 62-63 0.012149397758336528 64-65 0.015560191801148218 66-67 0.002514526995065552 68-69 9.709559683916488E-4 70-71 7.468892064551145E-5 72-73 2.4896306881837152E-5 74-75 0.0014688821060283917 76-77 0.002315356540010855 78-79 0.008439848032942794 80-81 0.014688821060283918 82-83 0.015410813959857196 84-85 0.025269751485064707 86-87 0.009709559683916487 88-89 0.03597516344425468 90-91 0.040556083910512714 92-93 0.026838218818620446 94-95 0.027709589559484746 96-97 0.043941981646442564 98-99 0.04643161233462628 100-101 0.05300423735143129 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2008330.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.06823568918218 #Duplication Level Percentage of deduplicated Percentage of total 1 79.51080108928565 46.17051937488323 2 12.271122272841653 14.251248406202844 3 3.5175741509978145 6.12777974562948 4 1.4820425883508637 3.442383932870543 5 0.7873797237900986 2.2860875688963307 6 0.4807759892736181 1.6750688075304105 7 0.33896513841274745 1.3778175283437375 8 0.23550194511387487 1.0940145963306664 9 0.1747294391143774 0.9131607227099048 >10 1.065553220307934 11.612054934893063 >50 0.08752917255567079 3.4766882069450125 >100 0.043809702803643914 4.610461197849449 >500 0.002925087411643708 1.1288341222220162 >1k 0.0011184157750402392 1.1176436972185744 >5k 1.7206396539080603E-4 0.7162371574746423 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7579 0.3773782197148875 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6758 0.3364984838149109 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3995 0.1989214919858788 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3589 0.17870569079782705 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.10028232412004003 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.97926137636743E-5 0.0 0.0 0.0 0.0 3 4.97926137636743E-5 0.0 0.0 0.0 0.0 4 4.97926137636743E-5 0.0 0.0 0.0 0.0 5 4.97926137636743E-5 0.0 0.0 0.0 0.0 6 4.97926137636743E-5 0.0 0.0 0.0 0.0 7 4.97926137636743E-5 0.0 0.0 0.0 0.0 8 4.97926137636743E-5 0.0 0.0 0.0 0.0 9 4.97926137636743E-5 0.0 0.0 0.0 0.0 10-11 4.97926137636743E-5 0.0 0.0 0.0 0.0 12-13 4.97926137636743E-5 0.0 0.0 0.0 0.0 14-15 7.468892064551145E-5 0.0 0.0 0.0 0.0 16-17 1.2448153440918576E-4 0.0 0.0 4.97926137636743E-5 0.0 18-19 1.493778412910229E-4 0.0 0.0 7.468892064551145E-5 4.97926137636743E-5 20-21 1.493778412910229E-4 0.0 0.0 9.95852275273486E-5 4.97926137636743E-5 22-23 1.493778412910229E-4 0.0 0.0 1.2448153440918576E-4 4.97926137636743E-5 24-25 1.493778412910229E-4 0.0 0.0 2.4896306881837146E-4 4.97926137636743E-5 26-27 1.493778412910229E-4 0.0 0.0 2.738593757002086E-4 4.97926137636743E-5 28-29 2.2406676193653433E-4 0.0 0.0 4.232372169912315E-4 4.97926137636743E-5 30-31 2.4896306881837146E-4 0.0 0.0 0.0010705411959189975 4.97926137636743E-5 32-33 2.4896306881837146E-4 0.0 0.0 0.002041497164310646 4.97926137636743E-5 34-35 3.236519894638829E-4 0.0 0.0 0.0031369346671114807 4.97926137636743E-5 36-37 3.4854829634572006E-4 4.97926137636743E-5 0.0 0.00445643893184885 4.97926137636743E-5 38-39 3.983409101093944E-4 9.95852275273486E-5 0.0 0.005701254275940707 4.97926137636743E-5 40-41 3.983409101093944E-4 9.95852275273486E-5 0.0 0.007493788371432981 4.97926137636743E-5 42-43 4.2323721699123154E-4 9.95852275273486E-5 0.0 0.010257278435316906 4.97926137636743E-5 44-45 4.979261376367429E-4 9.95852275273486E-5 0.0 0.01448965060522922 4.97926137636743E-5 46-47 5.228224445185801E-4 9.95852275273486E-5 0.0 0.025692988702055938 4.97926137636743E-5 48-49 5.975113651640916E-4 9.95852275273486E-5 0.0 0.04892124302280999 4.97926137636743E-5 50-51 5.975113651640916E-4 9.95852275273486E-5 0.0 0.06577604278181375 4.97926137636743E-5 52-53 5.975113651640916E-4 9.95852275273486E-5 0.0 0.08175947179995319 4.97926137636743E-5 54-55 5.975113651640916E-4 1.2448153440918576E-4 0.0 0.10551054856522583 4.97926137636743E-5 56-57 6.473039789277659E-4 1.493778412910229E-4 0.0 0.12405829719219451 4.97926137636743E-5 58-59 6.473039789277659E-4 1.493778412910229E-4 0.0 0.14427409838024627 4.97926137636743E-5 60-61 6.473039789277659E-4 1.493778412910229E-4 0.0 0.18089656580342872 4.97926137636743E-5 62-63 6.473039789277659E-4 1.493778412910229E-4 0.0 0.2081580218390404 4.97926137636743E-5 64-65 6.473039789277659E-4 1.493778412910229E-4 0.0 0.23693815259444415 4.97926137636743E-5 66-67 6.970965926914401E-4 1.493778412910229E-4 0.0 0.29519551069794303 4.97926137636743E-5 68-69 6.970965926914401E-4 1.493778412910229E-4 0.0 0.36435745121568663 4.97926137636743E-5 70-71 6.970965926914401E-4 1.493778412910229E-4 0.0 0.42099654937186615 9.95852275273486E-5 72-73 6.970965926914401E-4 1.493778412910229E-4 0.0 0.4825402199837676 9.95852275273486E-5 74-75 6.970965926914401E-4 1.493778412910229E-4 0.0 0.5344739161392799 9.95852275273486E-5 76-77 6.970965926914401E-4 1.493778412910229E-4 0.0 0.5791378906852958 9.95852275273486E-5 78-79 6.970965926914401E-4 1.493778412910229E-4 0.0 0.6402832203870878 9.95852275273486E-5 80-81 7.468892064551144E-4 1.493778412910229E-4 0.0 0.7454701169628497 9.95852275273486E-5 82-83 7.468892064551144E-4 1.493778412910229E-4 0.0 0.8462254709136446 9.95852275273486E-5 84-85 8.215781271006259E-4 1.493778412910229E-4 0.0 0.965404091956999 9.95852275273486E-5 86-87 8.962670477461373E-4 1.493778412910229E-4 0.0 1.1826741621147918 9.95852275273486E-5 88-89 8.962670477461373E-4 1.493778412910229E-4 0.0 1.4464754298347384 9.95852275273486E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2045 0.0 31.126415 2 GTACCTG 2195 0.0 30.518776 1 GTACATG 8900 0.0 23.754864 1 TATCACG 120 1.7107945E-4 23.751314 2 TACATGG 9105 0.0 23.007862 2 GGTATCA 11675 0.0 22.584923 1 ACATGGG 9020 0.0 22.2213 3 GTATCAA 15020 0.0 21.983528 1 GAGTACT 8460 0.0 21.697527 12-13 GTACTTT 8950 0.0 20.429512 14-15 CATGGGG 4280 0.0 19.639887 4 AGTACTT 8810 0.0 19.245245 12-13 ATCAACG 17405 0.0 18.965939 3 CAACGCA 17530 0.0 18.855448 5 TCAACGC 17505 0.0 18.855244 4 TATCAAC 17630 0.0 18.699274 2 AACGCAG 17845 0.0 18.628597 6 ATACCGA 130 0.0075745885 18.265238 6 CATGGGA 4885 0.0 17.88804 4 ACTTTTT 10600 0.0 17.65268 16-17 >>END_MODULE