FastQCFastQC Report
Thu 26 May 2016
SRR936439_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936439_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896873
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48680.25663289002479345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.20902822698198564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23230.12246470902374591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.037.5495641
GTATCAA71350.034.4260641
GTATACG1051.8992159E-631.6737541
ATCAACG87400.027.8315813
TCAACGC88450.027.6100714
TATCAAC88500.027.540062
CAACGCA90350.027.0827485
TACCTGG17650.026.9182032
GTACCTG17900.026.8076741
AACGCAG92400.026.4311646
GTACCGT1701.12035195E-725.1546766
GTACATG64800.024.2685471
GTATAGG5900.023.3526841
ACGCAGA104600.023.2562947
TACATGG66150.023.1268672
CGCAGAG105850.023.0720398
ACATGGG64900.022.7664343
GCAGAGT113850.020.8682029
GAGTACT62450.020.69427312-13
GTGTAGC10950.019.9588031
Produced by FastQC (version 0.11.5)