FastQCFastQC Report
Thu 26 May 2016
SRR936439_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936439_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896873
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59370.3129887978794574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52260.27550605654674826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.15794415335133138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42400.042.577511
GTATCAA72500.039.9063341
ATCAACG89600.032.233013
TCAACGC89900.031.965254
TATCAAC91550.031.5489442
CAACGCA92550.031.1013055
AACGCAG94000.030.6199346
ACGCAGA107650.026.5167277
CGCAGAG109800.025.9542488
TACCTGG17600.024.8321782
GTACATG62250.024.345231
TACATGG62650.023.7335622
GTACCTG18650.023.6900881
GCAGAGT118350.023.6779029
ACATGGG63300.022.5874963
GAGTACT75050.022.18443512-13
GTACTTT78250.021.21734414-15
GTGTAGC10200.020.959211
AGAGTAC103100.020.68678310-11
CAGAGTA114250.019.87362110-11
Produced by FastQC (version 0.11.5)