##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936439_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1896873 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.954163510155926 31.0 31.0 34.0 30.0 34.0 2 32.073293784032984 33.0 31.0 34.0 30.0 34.0 3 32.1990054157553 34.0 31.0 34.0 30.0 34.0 4 35.922819819777075 37.0 35.0 37.0 35.0 37.0 5 35.783580661435956 37.0 35.0 37.0 35.0 37.0 6 35.825209173202424 37.0 35.0 37.0 35.0 37.0 7 35.75357548976658 37.0 35.0 37.0 33.0 37.0 8 35.71982889734843 37.0 35.0 37.0 33.0 37.0 9 37.344002471435886 39.0 37.0 39.0 34.0 39.0 10-11 37.246570012858 39.0 37.0 39.0 33.5 39.0 12-13 37.120004080399696 39.0 37.0 39.0 33.0 39.0 14-15 38.24510497012715 40.0 38.0 41.0 33.0 41.0 16-17 38.21397663417635 40.0 37.5 41.0 33.0 41.0 18-19 38.338188692653645 40.0 38.0 41.0 33.5 41.0 20-21 38.333237122358746 40.0 38.0 41.0 34.0 41.0 22-23 38.23785830680283 40.0 38.0 41.0 33.5 41.0 24-25 38.12501285009592 40.0 37.0 41.0 33.0 41.0 26-27 37.9249949785779 40.0 37.0 41.0 33.0 41.0 28-29 37.95023203978337 40.0 37.0 41.0 33.0 41.0 30-31 37.827248318680276 40.0 37.0 41.0 33.0 41.0 32-33 37.642046146473696 40.0 37.0 41.0 33.0 41.0 34-35 37.47159430283419 40.0 36.0 41.0 32.0 41.0 36-37 37.294691579246475 39.5 36.0 41.0 32.0 41.0 38-39 37.09484952339983 39.0 36.0 41.0 31.0 41.0 40-41 36.86681053502264 39.0 35.5 41.0 31.0 41.0 42-43 36.61398470008272 39.0 35.0 40.5 30.0 41.0 44-45 36.34365558474394 39.0 35.0 40.0 30.0 41.0 46-47 35.99415722612953 39.0 35.0 40.0 29.5 41.0 48-49 35.615945031638915 38.0 35.0 40.0 28.0 41.0 50-51 35.306034457762856 38.0 34.5 40.0 27.0 41.0 52-53 35.02159685967379 38.0 34.0 40.0 26.5 41.0 54-55 34.68208757254703 38.0 34.0 40.0 26.0 41.0 56-57 34.3365707667303 37.5 33.0 40.0 25.5 41.0 58-59 33.95950256026629 37.0 33.0 40.0 24.5 41.0 60-61 33.577484892241074 36.5 32.5 39.0 23.5 40.5 62-63 33.22293348052295 36.0 32.0 39.0 23.0 40.0 64-65 32.891987497317956 36.0 32.0 39.0 22.0 40.0 66-67 32.43716685302601 35.0 31.0 38.5 21.5 40.0 68-69 31.982641431450602 35.0 31.0 38.0 20.0 40.0 70-71 31.472182639533592 35.0 30.5 37.0 18.5 39.5 72-73 30.900634096220465 34.0 30.0 36.5 17.5 39.0 74-75 30.361583774981245 34.0 29.0 36.0 13.5 39.0 76-77 29.003843694332723 32.5 28.0 34.5 12.0 36.5 78-79 29.779262238431357 34.0 29.0 35.0 10.5 37.0 80-81 29.633911179082627 34.0 29.0 35.0 7.5 37.0 82-83 29.23205322654706 34.0 29.0 35.0 7.0 36.0 84-85 28.792645843975848 33.5 29.0 35.0 2.0 36.0 86-87 28.361768816362506 33.0 27.5 35.0 2.0 35.5 88-89 27.85997902864346 33.0 27.0 35.0 2.0 35.0 90-91 27.286224749891005 32.5 26.0 34.0 2.0 35.0 92-93 26.689456806017063 32.0 25.0 34.0 2.0 35.0 94-95 25.93209403054395 32.0 24.5 34.0 2.0 35.0 96-97 25.074569831506906 31.0 21.0 34.0 2.0 35.0 98-99 23.88113964403521 31.0 8.5 34.0 2.0 35.0 100-101 20.008957373530016 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.261095144541148 1101 2 0.40800710209168045 1101 3 0.21410406422059225 1101 4 0.1252465838973862 1101 5 0.2099912013278029 1101 6 0.18259064147799364 1101 7 0.2442858173211846 1101 8 0.1633031027356182 1101 9 0.28819413622284173 1101 10-11 0.22345978166592317 1101 12-13 0.3967429408621044 1101 14-15 0.4558171549009131 1101 16-17 0.44846738820886145 1101 18-19 0.37323614204563427 1101 20-21 0.2648410565647694 1101 22-23 0.34379424814772364 1101 24-25 0.33780836058563324 1101 26-27 0.2560667420961167 1101 28-29 0.33543068164706824 1101 30-31 0.5563747473970722 1101 32-33 0.3654051469347266 1101 34-35 0.4421453673522677 1101 36-37 0.40671138935827145 1101 38-39 0.3700768182463605 1101 40-41 0.4735297087736825 1101 42-43 0.4166256146456746 1101 44-45 0.3397073366199237 1101 46-47 0.3861556933977681 1101 48-49 0.4934398285543793 1101 50-51 0.5008974487737703 1101 52-53 0.2619080722513516 1101 54-55 0.39864418843147575 1101 56-57 0.45460696284967383 1101 58-59 0.4808772070023011 1101 60-61 0.3488528129125612 1101 62-63 0.21803361742173166 1101 64-65 0.43763336174453826 1101 66-67 0.5399683726733855 1101 68-69 0.27089719314416527 1101 70-71 0.21023324055102321 1101 72-73 0.3587255396343352 1101 74-75 0.2814686757553062 1101 76-77 0.3027857163941725 1101 78-79 0.31047465959016307 1101 80-81 0.18099930227759842 1101 82-83 0.1742078922574919 1101 84-85 0.09749519884030278 1101 86-87 0.16350453246338148 1101 88-89 0.047823460122362604 1101 90-91 0.3471163873611651 1101 92-93 0.31868636299534714 1101 94-95 0.023280425010661077 1101 96-97 -0.08696439278326196 1101 98-99 -0.6992671403467234 1101 100-101 -0.20753372780393775 1102 1 0.20887493674923618 1102 2 0.3410709987751517 1102 3 0.29772167977316855 1102 4 0.23896889849363845 1102 5 0.07144512299760208 1102 6 0.11673136365917003 1102 7 0.1785621141640661 1102 8 0.1312573976674969 1102 9 0.2781547029940086 1102 10-11 0.3476274978848366 1102 12-13 0.29074589932574924 1102 14-15 0.34139365160847746 1102 16-17 0.3130813005006914 1102 18-19 0.3428463692109105 1102 20-21 0.333642282954159 1102 22-23 0.27169432616791767 1102 24-25 0.2559931540307261 1102 26-27 0.24049724432251907 1102 28-29 0.22918008526967526 1102 30-31 0.35496703768919247 1102 32-33 0.28788698791773726 1102 34-35 0.26046258356405616 1102 36-37 0.37215474656685643 1102 38-39 0.31619355407481464 1102 40-41 0.346446813503384 1102 42-43 0.2819869146426015 1102 44-45 0.4513812432901574 1102 46-47 0.47529160785965985 1102 48-49 0.5563185147063834 1102 50-51 0.34330053987606135 1102 52-53 0.6585956464881875 1102 54-55 0.6772072654063663 1102 56-57 0.6184753872087327 1102 58-59 0.47447443454365157 1102 60-61 0.3379098004694825 1102 62-63 0.3435826624191378 1102 64-65 0.4000817710522213 1102 66-67 0.3805762868658533 1102 68-69 0.38034361675799744 1102 70-71 0.16766927327373793 1102 72-73 0.14167547156635152 1102 74-75 0.20353127152649364 1102 76-77 0.37654426914761174 1102 78-79 0.4286865895559231 1102 80-81 0.610963763582042 1102 82-83 0.558981951039037 1102 84-85 0.44409149765203537 1102 86-87 0.7205428219429173 1102 88-89 0.4041990528837083 1102 90-91 0.48444574786757144 1102 92-93 0.42694974656758333 1102 94-95 0.4331242464545113 1102 96-97 0.35208799646109057 1102 98-99 0.26717510851740656 1102 100-101 0.33249965637709167 1103 1 0.23275391419732117 1103 2 0.23262162191944924 1103 3 0.2263986798912896 1103 4 0.14305946176425977 1103 5 0.11869714493010974 1103 6 0.10532077598521994 1103 7 0.1919176859678018 1103 8 0.20985990497812423 1103 9 0.04447929688225116 1103 10-11 0.20987835765334495 1103 12-13 0.11400139377889928 1103 14-15 0.26679500039612236 1103 16-17 0.2691863237164611 1103 18-19 0.3569905825444195 1103 20-21 0.26674577312953573 1103 22-23 0.23362456415116384 1103 24-25 0.30716943758688586 1103 26-27 0.24037150198761736 1103 28-29 0.3921997302867837 1103 30-31 0.4349471009668022 1103 32-33 0.355268897025077 1103 34-35 0.36390174226739447 1103 36-37 0.33140618593554905 1103 38-39 0.28722317634399275 1103 40-41 0.4552046189482368 1103 42-43 0.4846155009981672 1103 44-45 0.5043561291165446 1103 46-47 0.5296613021787664 1103 48-49 0.5899057953399804 1103 50-51 0.5396351296448643 1103 52-53 0.6513434467382311 1103 54-55 0.6556522984359532 1103 56-57 0.59402961666013 1103 58-59 0.6117843233527083 1103 60-61 0.45600884309190093 1103 62-63 0.7384146524545869 1103 64-65 0.5263520544127758 1103 66-67 0.5165366682927299 1103 68-69 0.5882288254373549 1103 70-71 0.3628893287104411 1103 72-73 0.6794635834541687 1103 74-75 0.6380194091978382 1103 76-77 0.5726505540204663 1103 78-79 0.4251208205992043 1103 80-81 0.5764311927186156 1103 82-83 0.4606036037844312 1103 84-85 0.5505517535482731 1103 86-87 0.4401094723661032 1103 88-89 0.5336409432016787 1103 90-91 0.57721289634895 1103 92-93 0.48084441428817115 1103 94-95 0.5797466887940317 1103 96-97 0.4922469864241421 1103 98-99 0.5659223846293671 1103 100-101 0.591678529293926 1104 1 0.1853368737997947 1104 2 0.2484257883878982 1104 3 0.16353599517021422 1104 4 0.09031518882035527 1104 5 0.07978408546906479 1104 6 0.08072611359717996 1104 7 0.07298499212149778 1104 8 0.13190339470899204 1104 9 0.23873905028475662 1104 10-11 0.2185884131399476 1104 12-13 0.17045684664555694 1104 14-15 0.21213096894651073 1104 16-17 0.18820421522566022 1104 18-19 0.17064112940962417 1104 20-21 0.20365322770880567 1104 22-23 0.23758474181207134 1104 24-25 0.06895071745472592 1104 26-27 0.11402996315348446 1104 28-29 0.17449094195324477 1104 30-31 0.2755557885335591 1104 32-33 0.27185270860417887 1104 34-35 0.25637796692974035 1104 36-37 0.29263789763012227 1104 38-39 0.38899113596808377 1104 40-41 0.3966370641211583 1104 42-43 0.30060368576897645 1104 44-45 0.42494239283142576 1104 46-47 0.5069771000743444 1104 48-49 0.3811648178589806 1104 50-51 0.40293299278368266 1104 52-53 0.5495412134350275 1104 54-55 0.24873636733794058 1104 56-57 0.5068948962338808 1104 58-59 0.6417160571035296 1104 60-61 0.4785407057203983 1104 62-63 0.32365123814743413 1104 64-65 0.4172410941875455 1104 66-67 0.4628338135223089 1104 68-69 0.47644711309406773 1104 70-71 0.31149254113805824 1104 72-73 0.31868229493682776 1104 74-75 0.5017972119630869 1104 76-77 0.3986216776349245 1104 78-79 0.43985558340804687 1104 80-81 0.4137161983624189 1104 82-83 0.44252591389527396 1104 84-85 0.49657352003747945 1104 86-87 0.5321361388021195 1104 88-89 0.26893364258367214 1104 90-91 0.2807001621094365 1104 92-93 0.5027806501083845 1104 94-95 0.6345008913741808 1104 96-97 0.6945019134006643 1104 98-99 0.5353297202778862 1104 100-101 0.3408535823802872 1105 1 0.10356922958451875 1105 2 0.22602900391408554 1105 3 0.14136837960104742 1105 4 0.08938060938110226 1105 5 0.12161636247697771 1105 6 0.08631926717615102 1105 7 0.0916926943451557 1105 8 0.09618616765273913 1105 9 0.009432286221304764 1105 10-11 0.1733700760468082 1105 12-13 0.05605053733915355 1105 14-15 0.1294999129791634 1105 16-17 0.17781328530121954 1105 18-19 0.22500026214854785 1105 20-21 0.06255590253960719 1105 22-23 0.020471374819557298 1105 24-25 -0.035606252502667246 1105 26-27 0.2012802048550597 1105 28-29 0.15510871328564235 1105 30-31 0.2081953488157282 1105 32-33 0.21565530107741182 1105 34-35 0.062294732067414316 1105 36-37 0.033310902249304775 1105 38-39 -0.0035725121144167815 1105 40-41 0.04006770471986698 1105 42-43 0.11714547328642766 1105 44-45 0.017663146940350316 1105 46-47 0.18799860256297052 1105 48-49 0.06162593076047784 1105 50-51 0.21633400255917223 1105 52-53 0.1306003139470775 1105 54-55 0.2112327865738024 1105 56-57 0.22378043710830298 1105 58-59 0.19675822060121817 1105 60-61 0.1478289393294503 1105 62-63 -0.019746280383017734 1105 64-65 -0.11669470271150573 1105 66-67 -0.08628352811839335 1105 68-69 0.02488410954911302 1105 70-71 -0.034317069632880504 1105 72-73 0.036133148949069493 1105 74-75 -0.01620955568112592 1105 76-77 -0.11867609871054796 1105 78-79 -0.26368221155739846 1105 80-81 -0.04393544699869878 1105 82-83 -0.10811325250283232 1105 84-85 -0.06436750477721276 1105 86-87 0.05093336012914662 1105 88-89 -0.026146835090266052 1105 90-91 0.06916875880797235 1105 92-93 0.09135980289958923 1105 94-95 0.167930905339162 1105 96-97 0.33570084275218903 1105 98-99 0.08743565718482316 1105 100-101 0.21238247914685715 1106 1 0.040162226522340916 1106 2 0.2271532809004313 1106 3 0.15203236871331427 1106 4 0.11447713447762453 1106 5 0.0817100768810306 1106 6 0.11790812204087331 1106 7 0.1231798465219569 1106 8 0.08594932982969539 1106 9 0.22773248296977755 1106 10-11 0.16486588219778753 1106 12-13 0.19039424064837362 1106 14-15 0.2269597496631448 1106 16-17 0.23858896245620542 1106 18-19 0.1862533871947747 1106 20-21 0.12619589721408886 1106 22-23 0.16106424601588287 1106 24-25 0.27190014552098063 1106 26-27 0.16544942333462842 1106 28-29 0.31883339856205595 1106 30-31 0.21138419036663691 1106 32-33 0.2735685098526872 1106 34-35 0.16584193690772508 1106 36-37 0.25231747039379826 1106 38-39 0.3472462869802513 1106 40-41 0.22765184057985977 1106 42-43 0.2293780876742204 1106 44-45 0.3003402205657011 1106 46-47 0.3405655784058155 1106 48-49 0.3883911195946226 1106 50-51 0.3293876350093541 1106 52-53 0.2568947117932012 1106 54-55 0.201152201062186 1106 56-57 0.24824733010967748 1106 58-59 0.22247328295421198 1106 60-61 0.2457316449907765 1106 62-63 0.3234753279247897 1106 64-65 0.4276143847010232 1106 66-67 0.22013002829516637 1106 68-69 0.19312069588914937 1106 70-71 0.263272391749684 1106 72-73 0.29574405295652895 1106 74-75 0.38570097553974847 1106 76-77 0.280879553000279 1106 78-79 0.2764579902207345 1106 80-81 0.2586522392096775 1106 82-83 0.34069238811315117 1106 84-85 0.41348164979607915 1106 86-87 0.36387530453660943 1106 88-89 0.5147690808256478 1106 90-91 0.6662615412455786 1106 92-93 0.44331397381927573 1106 94-95 0.395866044912232 1106 96-97 0.5134244407344042 1106 98-99 0.6993872023260259 1106 100-101 0.27017826754609686 1107 1 0.03442999052694873 1107 2 0.24087847225720793 1107 3 0.13155888771351698 1107 4 0.12731327889272137 1107 5 0.06861145194030627 1107 6 0.09629158068748467 1107 7 0.08155551542394335 1107 8 0.02279312851560178 1107 9 0.1336713165401875 1107 10-11 0.06982676413235112 1107 12-13 0.06501322053050984 1107 14-15 0.16144122361829716 1107 16-17 0.1824223635264488 1107 18-19 0.1444216118103725 1107 20-21 0.12149664909378544 1107 22-23 0.07523734768898294 1107 24-25 0.09762577716765719 1107 26-27 0.25708476168049543 1107 28-29 0.2714556636843142 1107 30-31 0.23634100808661884 1107 32-33 0.1468750871066291 1107 34-35 0.32731826285773735 1107 36-37 0.4110843199501275 1107 38-39 0.28652861442046884 1107 40-41 0.3525629358856932 1107 42-43 0.40733141694333597 1107 44-45 0.4435360479720458 1107 46-47 0.2810375353251402 1107 48-49 0.18054464530078462 1107 50-51 -0.010393405429574898 1107 52-53 0.020260050816439445 1107 54-55 0.23097337562020215 1107 56-57 0.24262516659278077 1107 58-59 0.02068502853438048 1107 60-61 -0.0739569345662261 1107 62-63 -0.2395115311146938 1107 64-65 -0.1458547611365404 1107 66-67 -0.0025550628109840545 1107 68-69 0.08512266025952897 1107 70-71 0.06351624462511651 1107 72-73 0.15874191924386594 1107 74-75 0.09747195704757416 1107 76-77 -0.11799347416222261 1107 78-79 -0.04963172731635268 1107 80-81 0.15508673761786085 1107 82-83 0.10579348545109113 1107 84-85 0.11151050571967147 1107 86-87 0.21557804110776857 1107 88-89 0.11715765360895958 1107 90-91 -0.09882262186489754 1107 92-93 0.07748385798915791 1107 94-95 0.39883376827469164 1107 96-97 0.3086557622815178 1107 98-99 0.6863055116127548 1107 100-101 0.4419844722470394 1108 1 0.04788591252471619 1108 2 0.22468500998954255 1108 3 -0.012687013219178311 1108 4 0.0546066401809 1108 5 0.0650330048760921 1108 6 0.04297314341737035 1108 7 0.10961071000200207 1108 8 0.166702666730572 1108 9 0.043673796861909864 1108 10-11 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2316 98-99 -1.3858952134119953 2316 100-101 -1.3223208417115764 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 10.0 4 18.0 5 57.0 6 133.0 7 301.0 8 755.0 9 1416.0 10 2328.0 11 3580.0 12 5134.0 13 7179.0 14 9028.0 15 11766.0 16 14683.0 17 18258.0 18 17043.0 19 12925.0 20 12402.0 21 13071.0 22 13965.0 23 15655.0 24 18255.0 25 21632.0 26 26306.0 27 32876.0 28 39899.0 29 49737.0 30 60745.0 31 75058.0 32 93651.0 33 119233.0 34 157063.0 35 212468.0 36 295367.0 37 353766.0 38 174753.0 39 6356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.982666578791275 18.782574309628945 14.930204969353456 23.304554142226323 2 16.052846220724394 19.88893182957737 39.36593181850535 24.692290131192884 3 19.77998514325059 23.628347183992247 29.53778006000693 27.053887612750234 4 13.697112132795816 16.659945056769924 35.71963664294861 33.92330616748565 5 14.2415108783388 36.635225182430375 33.800852088650544 15.32241185058028 6 33.124199669666865 35.313117957817944 16.86965864346216 14.693023729053026 7 29.33043873878481 31.96720490450316 20.06121666809885 18.641139688613183 8 27.06356457389103 34.38373662964519 19.107119415740076 19.445579380723704 9 26.24368169002798 16.604160120788585 19.882458874312814 37.26969931487062 10-11 25.490399199102942 26.108679916894808 26.707586643913427 21.69333424008882 12-13 26.2467228960505 23.301902657689787 27.465992715379468 22.985381730880245 14-15 23.00904993477394 24.964645603310093 25.626606433933475 26.39969802798249 16-17 22.68955942063063 27.91347365662925 26.504854394524635 22.892112528215485 18-19 22.55608140060237 26.365836672657327 28.957329581129887 22.120752345610413 20-21 23.126559646195076 25.53890907864004 29.160016437652768 22.174514837512117 22-23 22.747466344450633 25.469248190697908 29.14376043828129 22.63952502657017 24-25 22.74735853214414 25.63897637255636 29.23259572915689 22.381069366142608 26-27 22.45796163518979 25.899106870086147 29.00219493338626 22.640736561337803 28-29 22.437708616529903 26.12501690283185 29.017832784139568 22.419441696498684 30-31 22.607978908340005 25.767958527326822 28.9434110572532 22.680651507079975 32-33 22.42838430974977 25.826513324882296 28.979440992593613 22.76566137277432 34-35 22.923906717220497 25.917096500735955 28.778673763322043 22.38032301872151 36-37 22.959904662566693 25.740316204595903 28.66789344286278 22.631885689974624 38-39 22.699678085680034 25.91262656989906 28.752574101977142 22.635121242443766 40-41 22.9218621704938 25.820844574424477 28.660387031811574 22.596906223270146 42-43 22.83630135072513 25.836038280649902 28.412938830144775 22.914721538480194 44-45 22.875404532023964 25.98602745005313 28.415288878766525 22.72327913915638 46-47 23.219293015399558 26.229035892228946 27.836760816354072 22.714910276017424 48-49 23.198168513617528 26.367815607174112 27.57942240170391 22.854593477504455 50-51 23.178337668129874 26.51274382736307 27.608686884821992 22.700231619685063 52-53 23.4464201646826 26.480169079336864 27.351028884035255 22.72238187194528 54-55 23.052688847745127 26.54749503392954 27.44141407538305 22.958402042942275 56-57 23.099109729391586 26.75311816153623 27.29812584737307 22.84964626169912 58-59 23.21156923667384 26.411866997641052 27.3904767628617 22.986087002823403 60-61 23.038376425589906 26.477743465648114 27.737046951382656 22.746833157379324 62-63 22.928928106752817 26.531761830554366 27.74555214174102 22.7937579209518 64-65 22.928711646188717 26.48130677170693 27.58176746952032 23.00821411258403 66-67 22.88731763224792 26.61688775203574 27.444020467574205 23.051774148142133 68-69 23.05717360005103 26.406196209795567 27.560881615025433 22.975748575127962 70-71 22.98543368131031 26.458753894113844 27.360865005986483 23.19494741858936 72-73 23.013206671318347 26.56190813348647 27.265538155842467 23.159347039352724 74-75 22.97112841796257 26.82206982070327 27.06952018828055 23.13728157305361 76-77 23.022589711187624 26.646253737142413 27.15228034881226 23.178876202857705 78-79 23.030061468398262 26.744209955254973 27.10314027121171 23.122588305135057 80-81 23.186025368401715 26.739362460680667 26.953986129859707 23.120626041057914 82-83 23.086576837823326 26.84055156248888 26.989378855611427 23.083492744076363 84-85 23.061940664985098 26.814814853866125 26.983991001874436 23.13925347927434 86-87 23.10090196382339 26.878142362566365 26.83688999879274 23.1840656748175 88-89 22.991071722763955 26.847145922017084 26.74236631110134 23.419416044117618 90-91 22.897644279727647 26.816270268175618 26.95326967794726 23.332815774149473 92-93 22.903540593274354 26.58861183585869 27.174352564200564 23.33349500666639 94-95 22.8033263252899 26.556434079756734 27.163171079906455 23.47706851504691 96-97 22.91828336334766 26.36519491035768 27.130033022786986 23.58648870350767 98-99 22.68824652939778 26.535418251521858 26.915493544334563 23.860841674745803 100-101 22.50455299224311 26.493577836068027 27.038144440499263 23.963724731189597 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 214.0 1 166.5 2 206.5 3 279.5 4 226.5 5 202.5 6 322.0 7 679.5 8 1121.0 9 1355.5 10 1325.0 11 1246.5 12 1309.5 13 1435.5 14 1619.5 15 1835.0 16 2043.0 17 2222.5 18 2376.0 19 2654.5 20 2998.0 21 3371.5 22 3927.5 23 4681.0 24 5699.0 25 7076.5 26 8881.5 27 11141.5 28 13647.0 29 16415.0 30 19875.5 31 24158.0 32 28599.0 33 31422.0 34 33454.0 35 36251.0 36 39886.5 37 43627.5 38 46336.0 39 48293.5 40 50443.0 41 53824.0 42 56869.0 43 59505.5 44 62892.5 45 66557.0 46 68964.5 47 70311.5 48 72701.0 49 73137.0 50 71263.5 51 70471.0 52 70296.5 53 70393.0 54 69661.0 55 65430.0 56 59415.0 57 54408.5 58 51104.5 59 45871.0 60 37951.0 61 31520.0 62 26552.5 63 20595.0 64 14799.5 65 11067.0 66 8556.5 67 6962.0 68 5439.5 69 4211.5 70 3291.5 71 2372.5 72 1834.0 73 1527.0 74 1222.0 75 981.5 76 695.0 77 436.5 78 306.5 79 205.0 80 130.0 81 83.5 82 59.0 83 33.0 84 19.5 85 12.5 86 6.5 87 3.5 88 1.0 89 0.5 90 0.0 91 1.5 92 1.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01307414887554412 2 0.01054366844801945 3 0.004850087486088947 4 3.1631005344058354E-4 5 0.006431637753291865 6 0.0 7 2.1087336896038903E-4 8 0.0024250437430444736 9 2.635917112004863E-4 10-11 0.0 12-13 0.0 14-15 4.4810590904082663E-4 16-17 0.004560136603768412 18-19 0.0016869869516831122 20-21 4.4810590904082663E-4 22-23 4.7446508016087526E-4 24-25 5.799017646410698E-4 26-27 2.3723254008043763E-4 28-29 7.907751336014589E-5 30-31 5.008242512809239E-4 32-33 0.0034003330744862732 34-35 8.962118180816533E-4 36-37 0.0016606277805630634 38-39 0.005008242512809239 40-41 2.635917112004863E-5 42-43 0.0021087336896038903 44-45 0.004349263234808024 46-47 0.0 48-49 0.0027940721387251545 50-51 7.11697620241313E-4 52-53 6.326201068811671E-4 54-55 5.271834224009726E-5 56-57 0.010675464303619694 58-59 0.002609557940884814 60-61 0.0037166431279268566 62-63 2.635917112004863E-4 64-65 0.00403295318136744 66-67 0.0010016485025618478 68-69 0.001897860320643501 70-71 0.005271834224009725 72-73 0.0035057697589664675 74-75 0.0068006661489725465 76-77 0.001897860320643501 78-79 0.006642511122252254 80-81 0.0034003330744862732 82-83 0.002293247887444231 84-85 0.0017660644650432582 86-87 6.326201068811671E-4 88-89 0.0018187828072833553 90-91 0.00788139216489454 92-93 0.0023196070585642793 94-95 2.635917112004863E-4 96-97 5.271834224009726E-5 98-99 6.326201068811671E-4 100-101 0.002293247887444231 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1896873.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.73258165767191 #Duplication Level Percentage of deduplicated Percentage of total 1 81.65661778601428 52.041870609364594 2 11.066190539107316 14.105537843460263 3 3.0622917043992 5.855032683306999 4 1.3327161872739652 3.3974977292775663 5 0.7250114289976273 2.3103425050668336 6 0.46294269593738213 1.770271990299119 7 0.3093504204236053 1.380099065133779 8 0.22821288460246875 1.1635677042607755 9 0.17213293761984255 0.9873428852548402 >10 0.9108628164630699 10.413183502971112 >50 0.0506372305691639 2.173696489782312 >100 0.02046559584481463 2.4697386454784707 >500 0.001822290336781978 0.7852598213329718 >1k 5.798196526124447E-4 0.5572573226212316 >5k 1.6566275788926994E-4 0.5893012023892391 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5937 0.3129887978794574 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5226 0.27550605654674826 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2996 0.15794415335133138 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.271834224009726E-5 7 5.271834224009726E-5 0.0 0.0 0.0 1.0543668448019451E-4 8 5.271834224009726E-5 0.0 0.0 0.0 1.0543668448019451E-4 9 5.271834224009726E-5 0.0 0.0 0.0 1.0543668448019451E-4 10-11 5.271834224009726E-5 0.0 0.0 2.635917112004863E-5 1.0543668448019451E-4 12-13 5.271834224009726E-5 0.0 0.0 5.271834224009726E-5 1.0543668448019451E-4 14-15 5.271834224009726E-5 0.0 0.0 5.271834224009726E-5 1.5815502672029177E-4 16-17 5.271834224009726E-5 0.0 0.0 5.271834224009726E-5 2.1087336896038903E-4 18-19 5.271834224009726E-5 0.0 0.0 5.271834224009726E-5 2.1087336896038903E-4 20-21 5.271834224009726E-5 0.0 0.0 5.271834224009726E-5 2.1087336896038903E-4 22-23 5.271834224009726E-5 0.0 0.0 1.0543668448019451E-4 2.3723254008043766E-4 24-25 5.271834224009726E-5 0.0 0.0 1.0543668448019451E-4 2.635917112004863E-4 26-27 5.271834224009726E-5 0.0 0.0 1.0543668448019451E-4 2.635917112004863E-4 28-29 7.907751336014589E-5 0.0 0.0 3.4266922456063217E-4 2.635917112004863E-4 30-31 1.0543668448019451E-4 2.635917112004863E-5 0.0 0.0012125218715222368 2.899508823205349E-4 32-33 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.002609557940884814 3.1631005344058354E-4 34-35 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.0040856715236075375 3.1631005344058354E-4 36-37 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.00632620106881167 3.1631005344058354E-4 38-39 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.010016485025618478 3.1631005344058354E-4 40-41 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.01713346122803161 3.1631005344058354E-4 42-43 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.024065923232604397 3.1631005344058354E-4 44-45 1.0543668448019451E-4 5.271834224009726E-5 0.0 0.032079111253099174 3.690283956806808E-4 46-47 1.3179585560024314E-4 5.271834224009726E-5 0.0 0.04871174822984986 4.2174673792077805E-4 48-49 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.05941357170458961 4.744650801608753E-4 50-51 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.07327849571373518 4.744650801608753E-4 52-53 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.08687982801168027 5.00824251280924E-4 54-55 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.10385513421299158 5.271834224009726E-4 56-57 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.15196062150708034 5.535425935210211E-4 58-59 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.1898914687488303 5.799017646410698E-4 60-61 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.2167514641201599 5.799017646410698E-4 62-63 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.2510183865762231 5.799017646410698E-4 64-65 1.5815502672029177E-4 5.271834224009726E-5 0.0 0.28536438654564644 5.799017646410698E-4 66-67 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.34630679017519883 6.062609357611185E-4 68-69 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.42198397046085845 6.326201068811671E-4 70-71 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.46144364962757123 6.326201068811671E-4 72-73 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.5023267240347666 7.380567913613616E-4 74-75 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.5818787024750735 7.644159624814102E-4 76-77 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.6751638090689256 8.171343047215075E-4 78-79 2.1087336896038903E-4 5.271834224009726E-5 0.0 0.7716910937105436 8.434934758415561E-4 80-81 2.635917112004863E-4 5.271834224009726E-5 0.0 0.8536153975516547 8.698526469616047E-4 82-83 2.635917112004863E-4 5.271834224009726E-5 0.0 0.9781624810938845 8.962118180816534E-4 84-85 2.635917112004863E-4 5.271834224009726E-5 0.0 1.1901165760701957 8.962118180816534E-4 86-87 2.635917112004863E-4 5.271834224009726E-5 0.0 1.3814314400595085 0.001001648502561848 88-89 2.635917112004863E-4 7.907751336014589E-5 0.0 1.634874870378776 0.0010543668448019451 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4240 0.0 42.57751 1 GTATCAA 7250 0.0 39.906334 1 ATCAACG 8960 0.0 32.23301 3 TCAACGC 8990 0.0 31.96525 4 TATCAAC 9155 0.0 31.548944 2 CAACGCA 9255 0.0 31.101305 5 AACGCAG 9400 0.0 30.619934 6 ACGCAGA 10765 0.0 26.516727 7 CGCAGAG 10980 0.0 25.954248 8 TACCTGG 1760 0.0 24.832178 2 GTACATG 6225 0.0 24.34523 1 TACATGG 6265 0.0 23.733562 2 GTACCTG 1865 0.0 23.690088 1 GCAGAGT 11835 0.0 23.677902 9 ACATGGG 6330 0.0 22.587496 3 GAGTACT 7505 0.0 22.184435 12-13 GTACTTT 7825 0.0 21.217344 14-15 GTGTAGC 1020 0.0 20.95921 1 AGAGTAC 10310 0.0 20.686783 10-11 CAGAGTA 11425 0.0 19.873621 10-11 >>END_MODULE