FastQCFastQC Report
Thu 26 May 2016
SRR936438_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936438_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009432
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.26584626899541763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42450.2112537274214803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24970.1242639711122347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.040.6382941
GTATCAA66300.036.7736171
TCAACGC82700.029.0795974
TATCAAC83650.028.9751682
ATCAACG82900.028.9506683
CAACGCA85150.028.1877825
AACGCAG87750.027.5143936
TACCTGG18550.024.0827262
GTACATG67100.024.0817991
GTACCTG20750.024.0493721
ACGCAGA101000.023.9030447
CGCAGAG102400.023.4834258
TACATGG67800.022.8512332
ACATGGG67650.022.1291373
GCAGAGT111450.021.2769689
GAGTACT59250.020.77022612-13
CATGGGG43150.020.1564314
AGAGTAC92300.019.92500910-11
AGTACTT62100.019.3961812-13
GTGTAGC11450.019.093471
Produced by FastQC (version 0.11.5)