##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936438_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2009432 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.154400845612095 31.0 31.0 34.0 28.0 34.0 2 31.204306988243445 31.0 31.0 34.0 28.0 34.0 3 31.244844314214166 31.0 31.0 34.0 27.0 34.0 4 34.888965140397886 37.0 35.0 37.0 32.0 37.0 5 34.79738801810661 37.0 35.0 37.0 32.0 37.0 6 34.905661898486734 37.0 35.0 37.0 32.0 37.0 7 34.72503224791881 37.0 35.0 37.0 31.0 37.0 8 34.70785425931308 37.0 35.0 37.0 31.0 37.0 9 36.19303564390335 39.0 35.0 39.0 32.0 39.0 10-11 36.05282960558009 39.0 35.0 39.0 30.0 39.0 12-13 35.94925655608152 39.0 35.0 39.0 30.0 39.0 14-15 37.00805426608116 39.0 36.0 41.0 31.0 41.0 16-17 36.92274583066259 39.0 36.0 41.0 31.0 41.0 18-19 36.86496383057501 39.0 36.0 41.0 30.0 41.0 20-21 36.754079511026006 39.0 36.0 41.0 30.0 41.0 22-23 36.65104193622875 39.0 36.0 41.0 30.0 41.0 24-25 36.50550379410699 39.0 36.0 41.0 30.0 41.0 26-27 36.28604550937777 39.0 35.5 40.0 29.5 41.0 28-29 36.386995927207295 39.0 36.0 41.0 30.0 41.0 30-31 36.31576833652495 39.0 35.0 40.5 30.0 41.0 32-33 36.17169901743378 39.0 35.0 40.0 29.0 41.0 34-35 36.00517584073509 38.5 35.0 40.0 29.0 41.0 36-37 35.75994932896461 38.0 35.0 40.0 27.5 41.0 38-39 35.57086728986101 38.0 35.0 40.0 27.0 41.0 40-41 35.412067191126646 38.0 34.5 40.0 26.5 41.0 42-43 35.15102874842243 38.0 34.0 40.0 26.0 41.0 44-45 34.90066371989697 38.0 34.0 40.0 25.0 41.0 46-47 34.58166636143945 38.0 33.0 40.0 24.0 41.0 48-49 34.35153988788872 38.0 33.0 40.0 24.0 41.0 50-51 33.650006817847036 37.5 32.0 39.5 23.0 40.5 52-53 33.83077382066176 37.5 33.0 39.5 23.0 40.5 54-55 34.186530820649814 38.0 33.0 40.0 23.0 41.0 56-57 34.1096695484097 38.0 33.0 40.0 23.0 41.0 58-59 33.8611669864917 37.5 33.0 40.0 22.5 41.0 60-61 33.533771732509486 37.0 33.0 40.0 21.5 41.0 62-63 33.19911572026324 36.5 32.0 40.0 20.5 41.0 64-65 32.878466402446065 36.0 32.0 39.0 20.0 41.0 66-67 32.50903613558458 35.5 31.5 39.0 18.5 41.0 68-69 32.12555413669136 35.0 31.0 39.0 18.0 40.0 70-71 31.735503614951888 35.0 31.0 38.0 16.0 40.0 72-73 31.31867388396323 35.0 31.0 37.0 13.0 39.5 74-75 30.895429902579437 35.0 30.0 37.0 9.5 39.0 76-77 30.47130880766306 34.5 30.0 36.0 7.5 39.0 78-79 30.025325315810637 34.0 29.5 36.0 7.0 38.0 80-81 29.62718569227523 34.0 29.0 35.0 2.0 37.0 82-83 29.22557444093654 34.0 29.0 35.0 2.0 37.0 84-85 28.845613088673815 34.0 29.0 35.0 2.0 36.0 86-87 28.495552474530115 34.0 28.5 35.0 2.0 36.0 88-89 28.12386510217813 34.0 27.0 35.0 2.0 35.5 90-91 27.766801265233163 33.5 27.0 35.0 2.0 35.0 92-93 27.402902661050486 33.0 26.0 35.0 2.0 35.0 94-95 26.96575226233085 33.0 25.5 35.0 2.0 35.0 96-97 26.36876440705632 33.0 25.0 35.0 2.0 35.0 98-99 25.686601487385488 33.0 23.5 35.0 2.0 35.0 100-101 23.61152081782315 30.5 10.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39920226058013597 1101 2 0.5059803658766313 1101 3 0.5188091109500057 1101 4 0.4722113857308017 1101 5 0.5367720614052018 1101 6 0.4072788375255385 1101 7 0.2767664346523375 1101 8 0.29815755196244 1101 9 0.6204524762743588 1101 10-11 0.4817907494930722 1101 12-13 0.5149729415847943 1101 14-15 0.726646441582659 1101 16-17 0.7351961857791167 1101 18-19 0.731785984803139 1101 20-21 0.7323719247993239 1101 22-23 0.7449238957451811 1101 24-25 0.7113170381410185 1101 26-27 0.6428731264871814 1101 28-29 0.7297267140115693 1101 30-31 0.5271752180072937 1101 32-33 0.4433586902925768 1101 34-35 0.5500069734165081 1101 36-37 0.6718422311554733 1101 38-39 0.6080370621415767 1101 40-41 0.47556451800458177 1101 42-43 0.3432618715066553 1101 44-45 0.533088111552928 1101 46-47 0.39888828666993703 1101 48-49 0.41121398747782933 1101 50-51 0.5568902938248144 1101 52-53 0.7680804245992974 1101 54-55 0.8699679691584521 1101 56-57 0.709624022188521 1101 58-59 0.6830535012942249 1101 60-61 0.5520137238011955 1101 62-63 0.713698881828094 1101 64-65 0.7974769504428565 1101 66-67 0.6966020893539309 1101 68-69 0.7027144456140704 1101 70-71 0.6358587210977511 1101 72-73 0.5902809563119327 1101 74-75 0.5531790362620619 1101 76-77 0.5992969250551923 1101 78-79 0.5036464840339541 1101 80-81 0.36485421319803635 1101 82-83 0.47467189110395935 1101 84-85 0.4867878126709293 1101 86-87 0.4391556861293502 1101 88-89 0.5193474657510428 1101 90-91 0.6027761817013406 1101 92-93 0.4495982393562663 1101 94-95 0.3931184190028567 1101 96-97 0.2966287130275269 1101 98-99 0.2916534988419457 1101 100-101 0.6250667371655609 1102 1 0.1634977977530241 1102 2 0.21194362552173018 1102 3 0.09494919215333653 1102 4 0.2065771430622192 1102 5 0.177804958068549 1102 6 0.24497642349258086 1102 7 0.021160946253473867 1102 8 0.13192649248464505 1102 9 0.32989548920497214 1102 10-11 0.3762242140259815 1102 12-13 0.3816926642281757 1102 14-15 0.34012931613702335 1102 16-17 0.21050166318641317 1102 18-19 0.25271746959576546 1102 20-21 0.18075740234087334 1102 22-23 -0.21564942344735272 1102 24-25 -9.495903768055314E-4 1102 26-27 0.07399733704529154 1102 28-29 0.14045003450463156 1102 30-31 -0.011000857634812178 1102 32-33 -0.07858762640041306 1102 34-35 -0.1949232720981371 1102 36-37 -0.20742727866267785 1102 38-39 -0.32733022813559387 1102 40-41 -0.47245838472510115 1102 42-43 -0.018402989802453362 1102 44-45 -0.16012682053010252 1102 46-47 -0.006723536562198262 1102 48-49 0.1422145219531572 1102 50-51 0.08574042509430058 1102 52-53 -0.014673180592076562 1102 54-55 -0.14465186113514505 1102 56-57 0.04740540835072693 1102 58-59 0.03673374576005983 1102 60-61 -0.3154870502231333 1102 62-63 -0.05789597747568109 1102 64-65 0.02636769176938003 1102 66-67 -0.1166100025422665 1102 68-69 -0.05384525326739009 1102 70-71 0.035222657689001124 1102 72-73 -0.19011019656898753 1102 74-75 -0.29649337734297987 1102 76-77 -0.2154440222163423 1102 78-79 -0.23511065523924657 1102 80-81 -0.22048994025428215 1102 82-83 -0.3136191344929635 1102 84-85 -0.28946042217602397 1102 86-87 -0.29378190191853903 1102 88-89 -0.6096437938571384 1102 90-91 -0.503141496510942 1102 92-93 -0.3673563335962591 1102 94-95 -0.32390080281112077 1102 96-97 -0.561456547955018 1102 98-99 -0.5695634142399477 1102 100-101 -0.03730898892799672 1103 1 0.19006060538777447 1103 2 0.3480189128493798 1103 3 0.23311775592877382 1103 4 0.24204479305144844 1103 5 0.3344988952246837 1103 6 0.1789107217943311 1103 7 0.3595732876332747 1103 8 0.010484841744414553 1103 9 0.18704189516879666 1103 10-11 0.36235338330770617 1103 12-13 0.4188512980799217 1103 14-15 0.5151877712308419 1103 16-17 0.7013166839436451 1103 18-19 0.47132566248597385 1103 20-21 0.3710698191287207 1103 22-23 0.3398371842848391 1103 24-25 0.3745069280462232 1103 26-27 0.24058424322745253 1103 28-29 0.25938266881995276 1103 30-31 0.1791045103792399 1103 32-33 0.1988372001128269 1103 34-35 0.1742479764336835 1103 36-37 0.3856282623505294 1103 38-39 0.22790549848447483 1103 40-41 0.19850877838641878 1103 42-43 0.32734331272937567 1103 44-45 0.4674759940192743 1103 46-47 0.26303545538024764 1103 48-49 0.10453092655993146 1103 50-51 -0.38452533848247583 1103 52-53 -0.11143332408293105 1103 54-55 0.06718408150546651 1103 56-57 0.33906681411027506 1103 58-59 0.12248694238987667 1103 60-61 0.30500404442125273 1103 62-63 0.20190814637746257 1103 64-65 0.27484623743087155 1103 66-67 0.08871155128640851 1103 68-69 -0.027816440536589937 1103 70-71 0.22545202913327245 1103 72-73 0.20705164412643384 1103 74-75 -0.015289012504357657 1103 76-77 0.030884993785644355 1103 78-79 0.09298240399723312 1103 80-81 0.1596492474370237 1103 82-83 0.1372860802824185 1103 84-85 0.005534191681604739 1103 86-87 -0.17372502458359662 1103 88-89 -0.26971867221983103 1103 90-91 -0.25307071806298254 1103 92-93 -0.23903659855298898 1103 94-95 -0.15413815646295248 1103 96-97 -0.14691123301607334 1103 98-99 -0.14928006640190716 1103 100-101 -0.23178588846447923 1104 1 0.30519340256770633 1104 2 0.3255238072374418 1104 3 0.30082419386664583 1104 4 0.31911764773492024 1104 5 0.2038964577109752 1104 6 0.2886880100663589 1104 7 0.42198547182775314 1104 8 0.36866788904625025 1104 9 0.5188044854147265 1104 10-11 0.5703154701767659 1104 12-13 0.5040965958924843 1104 14-15 0.4067455442902883 1104 16-17 0.20874814041873435 1104 18-19 0.39724416861511 1104 20-21 0.4144528354213861 1104 22-23 0.47248132672458354 1104 24-25 0.3839629179176356 1104 26-27 0.39398284042393783 1104 28-29 0.35104298904990827 1104 30-31 0.2874741348870913 1104 32-33 0.11046256674004695 1104 34-35 0.27316864035130095 1104 36-37 0.3720323271227457 1104 38-39 0.4300067715475535 1104 40-41 0.21586929535590826 1104 42-43 0.314651443478823 1104 44-45 0.09544385694909607 1104 46-47 0.37518251517251855 1104 48-49 0.4946177723246521 1104 50-51 0.3427274055480609 1104 52-53 0.2997474907192057 1104 54-55 0.6234206405156826 1104 56-57 0.618248272392897 1104 58-59 0.6423018835085941 1104 60-61 0.5534008662221481 1104 62-63 0.3992209726867415 1104 64-65 0.28543835997654554 1104 66-67 0.20595504076618454 1104 68-69 0.29350907302269036 1104 70-71 0.3930579081830423 1104 72-73 0.1996054261608755 1104 74-75 0.07555922491343026 1104 76-77 0.09564274868947109 1104 78-79 0.09891320936005599 1104 80-81 0.2762018589957336 1104 82-83 0.05042505198293412 1104 84-85 0.1829486292627962 1104 86-87 -0.006268467549613632 1104 88-89 0.12079846597101351 1104 90-91 -0.08410186139364129 1104 92-93 0.06859125603809346 1104 94-95 0.21425502216192527 1104 96-97 0.228975561013133 1104 98-99 0.3957495087965377 1104 100-101 0.4329434078924237 1105 1 0.2340538474935947 1105 2 0.2390336719117947 1105 3 0.22747434639673614 1105 4 0.3532314200469244 1105 5 0.4009079830914999 1105 6 0.3644313453378416 1105 7 0.4335040820914742 1105 8 0.05571000009452831 1105 9 0.436177383645699 1105 10-11 0.5050612666387053 1105 12-13 0.4658159246007969 1105 14-15 0.5832887664585371 1105 16-17 0.48685728505549264 1105 18-19 0.34992897693695824 1105 20-21 0.034629618987963795 1105 22-23 -0.05542610384037516 1105 24-25 0.06763941076106761 1105 26-27 0.14954321924017933 1105 28-29 0.19756446671997452 1105 30-31 0.10745318689068739 1105 32-33 0.03909342003239402 1105 34-35 -0.053504212479069224 1105 36-37 -0.02328284862537089 1105 38-39 0.001896631283585748 1105 40-41 -0.1698952268625078 1105 42-43 -0.023811228465994816 1105 44-45 0.2675360281332573 1105 46-47 0.1549806418003712 1105 48-49 0.2671529976151774 1105 50-51 0.28599517351116077 1105 52-53 0.3376928347681414 1105 54-55 0.36165252607430887 1105 56-57 0.4194519856841268 1105 58-59 0.19557391275614577 1105 60-61 0.3937594114913239 1105 62-63 0.07792390938874405 1105 64-65 0.1611167803542486 1105 66-67 -0.10214359004727669 1105 68-69 0.09412795489351566 1105 70-71 0.2695625000157129 1105 72-73 0.2939912834755489 1105 74-75 0.30684501771146344 1105 76-77 0.24204495022035033 1105 78-79 0.23437764953878926 1105 80-81 0.3585834390959306 1105 82-83 0.2586728277517558 1105 84-85 0.060808269721896124 1105 86-87 0.22852949977788128 1105 88-89 0.3675989183840187 1105 90-91 0.3894299867294002 1105 92-93 0.36180864526460965 1105 94-95 0.45036044713544143 1105 96-97 0.3805900700892728 1105 98-99 0.26472218082100696 1105 100-101 0.38011323799859653 1106 1 0.06704297760241218 1106 2 -0.06656181299491237 1106 3 0.032289126409889235 1106 4 0.16471128065960983 1106 5 0.3486705288492047 1106 6 0.20558438948715718 1106 7 0.48355436308526123 1106 8 0.4116690475745841 1106 9 0.4336643335786192 1106 10-11 0.44874225522501376 1106 12-13 0.47589181591388297 1106 14-15 0.38238130947726745 1106 16-17 0.25439825456901133 1106 18-19 0.3582518308981619 1106 20-21 0.3369784311369699 1106 22-23 0.4934029387815144 1106 24-25 0.21424069547544633 1106 26-27 0.2811387761196755 1106 28-29 0.17870249654470172 1106 30-31 0.22712435119058938 1106 32-33 0.30546563133652427 1106 34-35 0.1645007816331585 1106 36-37 0.14465876944299794 1106 38-39 0.22645084978898922 1106 40-41 0.1724555645383461 1106 42-43 0.18432789494535484 1106 44-45 0.004005642618977845 1106 46-47 0.15564189184784993 1106 48-49 0.12946724283047928 1106 50-51 0.3974711355974918 1106 52-53 0.32429989729850917 1106 54-55 0.5151102190663579 1106 56-57 0.4485720782891178 1106 58-59 0.3249645031129944 1106 60-61 0.2576422637731639 1106 62-63 0.20163794548309966 1106 64-65 0.16387563750624423 1106 66-67 0.1599525376164621 1106 68-69 -0.024532491457769368 1106 70-71 0.07444945274757941 1106 72-73 0.3291159063680986 1106 74-75 0.2711456120867872 1106 76-77 0.06327522517641526 1106 78-79 0.3672383282022551 1106 80-81 0.3102451109839599 1106 82-83 0.4424027778306261 1106 84-85 0.30245462739869566 1106 86-87 0.4651252665780312 1106 88-89 0.5958275479620383 1106 90-91 0.608776673681735 1106 92-93 0.4028508313182826 1106 94-95 0.4550675678438161 1106 96-97 0.6234736600922481 1106 98-99 0.8141781864508779 1106 100-101 1.067041379161239 1107 1 0.6682136712274094 1107 2 0.6536256195754895 1107 3 0.6968704211739762 1107 4 0.6258320257192764 1107 5 0.6686156491175552 1107 6 0.5786005841707578 1107 7 0.7928371630613285 1107 8 0.7849404856030446 1107 9 0.8887767275006482 1107 10-11 0.962857356946003 1107 12-13 1.0069295755238628 1107 14-15 1.1174726132544848 1107 16-17 1.1776848431476168 1107 18-19 1.18651998309074 1107 20-21 1.244260682991225 1107 22-23 1.2501436067181189 1107 24-25 1.266116251558536 1107 26-27 1.3317154379034264 1107 28-29 1.0253377957825407 1107 30-31 1.0664373268165335 1107 32-33 1.094969363968879 1107 34-35 0.9641095452131694 1107 36-37 1.2202134532790012 1107 38-39 1.2471558376975054 1107 40-41 0.9666324457638211 1107 42-43 1.0115279092274037 1107 44-45 1.0170858990396212 1107 46-47 0.9556559567694052 1107 48-49 0.9966208018587608 1107 50-51 0.9599252979457731 1107 52-53 0.8265754954557352 1107 54-55 0.9345154441726464 1107 56-57 1.1039194987390815 1107 58-59 1.2632886158407999 1107 60-61 1.1264729983528952 1107 62-63 1.2833475403168606 1107 64-65 1.0521690390074099 1107 66-67 1.1275110602351717 1107 68-69 1.2256362634758133 1107 70-71 1.3087288269623123 1107 72-73 1.2537943914288796 1107 74-75 1.0937114397796783 1107 76-77 1.3526447017393828 1107 78-79 1.399671361805595 1107 80-81 1.3653698328217878 1107 82-83 1.4330152267578349 1107 84-85 1.6252279307783297 1107 86-87 1.5046323767215668 1107 88-89 1.4721725211638983 1107 90-91 1.3701807195541242 1107 92-93 1.3629736424497843 1107 94-95 1.4562635686775813 1107 96-97 1.5574767297674121 1107 98-99 1.6725563683076636 1107 100-101 2.0796682047060315 1108 1 0.11455899257272861 1108 2 0.1868840278338979 1108 3 0.10146582576938101 1108 4 -0.0014157265284779896 1108 5 0.18364228914419556 1108 6 0.17800118357136085 1108 7 0.3463253415495089 1108 8 0.12168707234962994 1108 9 0.35173044045436086 1108 10-11 0.3115919556806048 1108 12-13 0.32192707802136766 1108 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2316 90-91 -3.2622673146430152 2316 92-93 -3.4944811038119816 2316 94-95 -3.5817809406316634 2316 96-97 -4.109191185010449 2316 98-99 -4.764470736229693 2316 100-101 -5.2972344714662505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5754.0 3 2241.0 4 2379.0 5 2656.0 6 2940.0 7 3462.0 8 4364.0 9 5373.0 10 6551.0 11 7885.0 12 9527.0 13 10810.0 14 11722.0 15 13586.0 16 15753.0 17 18104.0 18 16741.0 19 13487.0 20 14306.0 21 15291.0 22 17117.0 23 19471.0 24 22537.0 25 25318.0 26 29660.0 27 35122.0 28 41969.0 29 49933.0 30 59677.0 31 72708.0 32 89688.0 33 112192.0 34 146954.0 35 197528.0 36 270756.0 37 342645.0 38 255858.0 39 37356.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47338963875966 18.01164764794857 14.11843052896209 23.396532184329676 2 15.863508480826876 19.376976126476077 40.024993816245185 24.73452157645186 3 19.68561387861833 23.562933215345634 29.060211053980865 27.69124185205517 4 13.202365075263936 16.045752268471986 36.25652215254308 34.49536050372101 5 14.444046143199255 36.43137020607111 33.752694756861985 15.371888893867656 6 33.01398881372947 34.88962122841823 17.338071961820077 14.758317996032218 7 29.4845686187347 31.793090711602247 20.164959148492603 18.557381521170456 8 26.94465945717237 34.27081648145152 19.4161554064985 19.368368654877607 9 26.414418181003718 16.9648554349395 19.931512644776596 36.689213739280184 10-11 25.496273776828904 26.076019134982488 26.81055909280108 21.61714799538753 12-13 26.260275190007466 23.305715421578714 27.342205617564403 23.091803770849413 14-15 23.139954301022417 24.966519687618295 25.505564538668544 26.38796147269074 16-17 22.83888457209758 28.048842405812785 26.222989012673782 22.88928400941586 18-19 22.73630462524819 26.577473647686826 28.40962181453059 22.27659991253439 20-21 23.343210030177644 25.834551846436238 28.517163923715895 22.305074199670223 22-23 22.976801760156526 25.728601722279613 28.505163528963518 22.789432988600346 24-25 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27.260037005813636 23.00026276317374 50-51 23.14422606776409 26.720372649015438 27.261455237204185 22.87394604601628 52-53 23.39307971168659 26.67088183325408 27.019018761970237 22.91701969308909 54-55 23.0093531710405 26.723772402521785 27.166721741917378 23.100152684520335 56-57 23.080370362075957 26.888177228355943 26.982509573824824 23.048942835743276 58-59 23.15793193097147 26.635461228682395 27.036506928532894 23.170099911813242 60-61 23.03652089181335 26.657008780929143 27.394065376852872 22.912404950404632 62-63 22.96787229039478 26.77507368483915 27.342555809500585 22.914498215265482 64-65 22.893073967296072 26.727203324838094 27.233035776050198 23.146686931815637 66-67 22.848469239851646 26.900296236023348 27.071244701406282 23.179989822718728 68-69 23.016069522702054 26.71643688935528 27.212256205580005 23.055237382362666 70-71 22.961146167286014 26.733650684551414 27.018759125050302 23.286444023112274 72-73 23.01541484311936 26.767128073488 26.984112369996048 23.23334471339659 74-75 22.97234988067194 27.064315721817188 26.862486945577395 23.100847451933472 76-77 23.140391192750954 26.866961867353783 26.805051838451654 23.187595101443613 78-79 23.064100202615585 26.985060212173863 26.81843752022422 23.132402064986334 80-81 23.18391323252307 27.027303491789638 26.721398258946007 23.067385016741287 82-83 23.118673961880287 27.071387122428405 26.811527485948222 22.99841142974309 84-85 23.07136991414707 27.14122184894861 26.678387458006718 23.109020778897598 86-87 23.190158889814313 27.08674302382613 26.704492716440743 23.01860536991881 88-89 23.052657170364828 27.08931953455629 26.494612450004567 23.363410845074313 90-91 22.962105600033446 27.086558640596603 26.711927355275833 23.239408404094117 92-93 23.040300424057815 26.954523283362203 26.89469728642816 23.110479006151824 94-95 22.91121695333652 26.881519748367577 26.930616937410417 23.276646360885493 96-97 23.002440346869165 26.77618159744056 26.879847194024126 23.34153086166615 98-99 22.785199838124473 26.906977820763174 26.770214903200724 23.537607437911625 100-101 22.80414479602866 26.77324552385229 26.88297418439095 23.5396354957281 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 199.0 1 144.0 2 179.0 3 256.5 4 226.0 5 217.5 6 326.5 7 628.0 8 1031.0 9 1284.0 10 1201.0 11 1159.5 12 1297.5 13 1389.0 14 1519.5 15 1659.0 16 1805.5 17 1980.0 18 2174.0 19 2439.0 20 2733.0 21 3163.5 22 3776.0 23 4609.5 24 5775.0 25 7227.5 26 9064.0 27 11455.0 28 13991.5 29 16912.0 30 20696.0 31 25208.5 32 30173.5 33 33195.0 34 35441.5 35 38574.5 36 42589.5 37 46481.0 38 49213.5 39 51563.5 40 53659.5 41 56910.5 42 60315.5 43 63391.5 44 66691.0 45 70788.5 46 73521.0 47 74852.0 48 77397.5 49 77418.0 50 75612.5 51 74732.5 52 74141.5 53 74042.0 54 73419.0 55 69289.0 56 63437.5 57 58265.5 58 54261.0 59 48705.5 60 40373.0 61 33525.5 62 28298.5 63 22340.5 64 16406.5 65 12313.0 66 9435.0 67 7583.5 68 6043.5 69 4680.5 70 3636.0 71 2725.5 72 2083.5 73 1678.0 74 1299.5 75 1041.5 76 790.0 77 508.0 78 337.5 79 212.5 80 121.5 81 78.0 82 57.0 83 43.0 84 33.5 85 20.5 86 14.0 87 13.0 88 8.0 89 4.0 90 3.0 91 2.5 92 3.5 93 2.5 94 1.5 95 2.0 96 2.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013486398146341852 2 0.006718316419764391 3 0.00910705114679173 4 0.03488548007596176 5 0.04488830674538875 6 0.06250522535721537 7 0.02841599019026272 8 0.02523101055422627 9 0.015029122657547007 10-11 0.04797375576779906 12-13 0.044415536330664585 14-15 0.020677484980830402 16-17 0.023937112577086462 18-19 0.03197420962739719 20-21 0.0246089442190629 22-23 0.014556352242822847 24-25 0.0034835714769148696 26-27 0.0029112704485645693 28-29 0.0014680765509855521 30-31 0.0068676123402036 32-33 0.0015427245112051566 34-35 0.001791551045270504 36-37 0.00624554600504023 38-39 0.006942260300423204 40-41 0.00395634189163903 42-43 6.718316419764391E-4 44-45 0.0016422551248312957 46-47 0.0027868571815318956 48-49 8.95775522635252E-4 50-51 0.0017169030850509 52-53 0.0010699540964809956 54-55 0.0029859184087841738 56-57 0.0023389694202142694 58-59 0.0029859184087841738 60-61 0.001020188789667926 62-63 0.0014680765509855521 64-65 0.003533336783727939 66-67 0.002712209221312291 68-69 0.006544137845918648 70-71 0.009778882788768168 72-73 0.0034835714769148696 74-75 0.002761974528125361 76-77 0.0032098622894429865 78-79 0.03493524538277483 80-81 0.023713168696427648 82-83 0.003881693931419426 84-85 0.009057285839978661 86-87 0.00729061744811469 88-89 0.006146015391414091 90-91 0.012764801197552342 92-93 0.01353616345315492 94-95 0.007564326635586574 96-97 0.0043046990393305175 98-99 0.023937112577086462 100-101 0.0418526230297915 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2009432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.67549971870753 #Duplication Level Percentage of deduplicated Percentage of total 1 82.83817267649394 54.40438386313323 2 10.35668395684957 13.603607885896334 3 2.913760869998801 5.740881035939617 4 1.251959502629989 3.288922638512362 5 0.6702428237952666 2.200926619281588 6 0.4216861069018741 1.6616667477130143 7 0.2868026583455818 1.318513553524988 8 0.19614697887518429 1.0305640684753998 9 0.1514776580392431 0.8953533799152107 >10 0.8477139944698923 9.939170492063035 >50 0.04392228878365943 1.9747445987335757 >100 0.01930386767160143 2.3696163273031026 >500 0.0015190122466579629 0.6767846507570173 >1k 5.316542863302807E-4 0.6284001348936339 >5k 7.595061233289724E-5 0.2664640038578975 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5342 0.26584626899541763 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4245 0.2112537274214803 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2497 0.1242639711122347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 9.953061362613912E-5 0.0 0.0 0.0 0.0 7 9.953061362613912E-5 0.0 0.0 0.0 0.0 8 9.953061362613912E-5 0.0 0.0 0.0 0.0 9 9.953061362613912E-5 0.0 0.0 0.0 0.0 10-11 9.953061362613912E-5 0.0 0.0 0.0 0.0 12-13 9.953061362613912E-5 0.0 0.0 0.0 4.976530681306956E-5 14-15 1.4929592043920868E-4 0.0 0.0 0.0 4.976530681306956E-5 16-17 1.4929592043920868E-4 0.0 0.0 4.976530681306956E-5 9.953061362613912E-5 18-19 1.4929592043920868E-4 0.0 0.0 7.464796021960434E-5 1.4929592043920868E-4 20-21 1.4929592043920868E-4 0.0 0.0 1.4929592043920868E-4 1.4929592043920868E-4 22-23 1.4929592043920868E-4 0.0 0.0 2.737091874718826E-4 1.7417857384574348E-4 24-25 2.4882653406534783E-4 0.0 0.0 3.4835714769148696E-4 1.9906122725227824E-4 26-27 2.4882653406534783E-4 0.0 0.0 3.981224545045565E-4 3.4835714769148696E-4 28-29 2.4882653406534783E-4 0.0 0.0 7.96244909009113E-4 4.478877613176261E-4 30-31 2.4882653406534783E-4 2.488265340653478E-5 0.0 0.0017666683918639695 5.225357215372304E-4 32-33 2.4882653406534783E-4 4.976530681306956E-5 0.0 0.0031600969826299174 5.723010283503E-4 34-35 2.737091874718826E-4 4.976530681306956E-5 0.0 0.004653056187022005 6.967142953829739E-4 36-37 3.4835714769148696E-4 4.976530681306956E-5 0.0 0.006718316419764392 6.967142953829739E-4 38-39 3.981224545045565E-4 4.976530681306956E-5 0.0 0.009977944016020448 6.967142953829739E-4 40-41 3.981224545045565E-4 4.976530681306956E-5 0.0 0.01692020431644365 6.967142953829739E-4 42-43 3.981224545045565E-4 7.464796021960434E-5 0.0 0.02279251052038586 6.967142953829739E-4 44-45 3.981224545045565E-4 9.953061362613912E-5 0.0 0.030506133076411642 6.967142953829739E-4 46-47 4.478877613176261E-4 9.953061362613912E-5 0.0 0.04498783735901489 7.215969487895087E-4 48-49 4.478877613176261E-4 9.953061362613912E-5 0.0 0.054965781375035334 7.464796021960434E-4 50-51 4.976530681306957E-4 9.953061362613912E-5 0.0 0.06653621520907402 7.464796021960434E-4 52-53 4.976530681306957E-4 9.953061362613912E-5 0.0 0.07957472559409823 8.211275624156477E-4 54-55 4.976530681306957E-4 9.953061362613912E-5 0.0 0.09651981256394843 8.957755226352522E-4 56-57 4.976530681306957E-4 1.4929592043920868E-4 0.0 0.13727759884385238 9.206581760417869E-4 58-59 4.976530681306957E-4 1.9906122725227824E-4 0.0 0.17196401769256187 9.455408294483217E-4 60-61 4.976530681306957E-4 1.9906122725227824E-4 0.0 0.1971701455933816 9.953061362613913E-4 62-63 4.976530681306957E-4 1.9906122725227824E-4 0.0 0.22668097253353187 9.953061362613913E-4 64-65 4.976530681306957E-4 1.9906122725227824E-4 0.0 0.2580331158257657 9.953061362613913E-4 66-67 5.971836817568347E-4 1.9906122725227824E-4 0.0 0.31349655026893175 9.953061362613913E-4 68-69 5.971836817568347E-4 1.9906122725227824E-4 0.0 0.38232196959140696 9.953061362613913E-4 70-71 5.971836817568347E-4 1.9906122725227824E-4 0.0 0.41720744966736867 0.0010948367498875304 72-73 6.220663351633696E-4 1.9906122725227824E-4 0.0 0.454581195083984 0.0013934285907659478 74-75 6.718316419764391E-4 1.9906122725227824E-4 0.0 0.5293038032638079 0.0014431938975790174 76-77 6.967142953829739E-4 1.9906122725227824E-4 0.0 0.617761636124039 0.0014431938975790174 78-79 6.967142953829739E-4 1.9906122725227824E-4 0.0 0.7067171220524009 0.0014929592043920869 80-81 7.464796021960434E-4 1.9906122725227824E-4 0.0 0.7813899649254117 0.0015676071646116914 82-83 7.464796021960434E-4 1.9906122725227824E-4 0.0 0.901075527810844 0.0016422551248312957 84-85 7.464796021960434E-4 1.9906122725227824E-4 0.0 1.1032719693923458 0.0016920204316443652 86-87 7.464796021960434E-4 1.9906122725227824E-4 0.0 1.2855622882486195 0.0017169030850509 88-89 7.464796021960434E-4 1.9906122725227824E-4 0.0 1.527446562013544 0.0017417857384574348 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4140 0.0 40.638294 1 GTATCAA 6630 0.0 36.773617 1 TCAACGC 8270 0.0 29.079597 4 TATCAAC 8365 0.0 28.975168 2 ATCAACG 8290 0.0 28.950668 3 CAACGCA 8515 0.0 28.187782 5 AACGCAG 8775 0.0 27.514393 6 TACCTGG 1855 0.0 24.082726 2 GTACATG 6710 0.0 24.081799 1 GTACCTG 2075 0.0 24.049372 1 ACGCAGA 10100 0.0 23.903044 7 CGCAGAG 10240 0.0 23.483425 8 TACATGG 6780 0.0 22.851233 2 ACATGGG 6765 0.0 22.129137 3 GCAGAGT 11145 0.0 21.276968 9 GAGTACT 5925 0.0 20.770226 12-13 CATGGGG 4315 0.0 20.156431 4 AGAGTAC 9230 0.0 19.925009 10-11 AGTACTT 6210 0.0 19.39618 12-13 GTGTAGC 1145 0.0 19.09347 1 >>END_MODULE