FastQCFastQC Report
Thu 26 May 2016
SRR936438_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936438_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2009432
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60160.2993880857874265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54440.27092233029035073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31790.15820391035874815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.041.1164741
GTATCAA74100.035.5775531
TCAACGC92600.028.2113844
ATCAACG93050.028.075653
TATCAAC94950.027.6714922
CAACGCA94550.027.6240525
AACGCAG97900.026.6787956
TACCTGG17500.023.6202012
ACGCAGA113450.022.8127787
GTACATG66900.022.753861
CGCAGAG114550.022.5108018
GTACCTG19150.022.0666221
ACATGGG66250.021.5089323
TACATGG68800.021.3389192
GAGTACT74250.021.1058912-13
GCAGAGT124750.020.556049
TAAGGTG9400.020.207775
GTACTTT78700.020.00299614-15
GTGTAGC10350.019.4863681
AGAGTAC106550.019.3875110-11
Produced by FastQC (version 0.11.5)