##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936438_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2009432 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.488912787295117 31.0 31.0 34.0 30.0 34.0 2 31.709419875865418 31.0 31.0 34.0 30.0 34.0 3 31.993652932769063 33.0 31.0 34.0 30.0 34.0 4 35.79849430087707 37.0 35.0 37.0 35.0 37.0 5 35.598944378311884 37.0 35.0 37.0 33.0 37.0 6 35.696904896508066 37.0 35.0 37.0 33.0 37.0 7 35.62868860454098 37.0 35.0 37.0 33.0 37.0 8 35.60132614589595 37.0 35.0 37.0 33.0 37.0 9 37.245991902189274 39.0 37.0 39.0 33.0 39.0 10-11 37.13357157644548 39.0 37.0 39.0 33.0 39.0 12-13 37.00010127239936 39.0 37.0 39.0 33.0 39.0 14-15 38.150533832446186 40.0 37.0 41.0 33.0 41.0 16-17 38.04979964487477 40.0 37.0 41.0 33.0 41.0 18-19 38.09140592963584 40.0 37.0 41.0 33.0 41.0 20-21 38.15787570816032 40.0 37.0 41.0 33.0 41.0 22-23 38.07340432520235 40.0 37.0 41.0 33.0 41.0 24-25 37.968930772476995 40.0 37.0 41.0 33.0 41.0 26-27 37.76421247397275 40.0 37.0 41.0 32.5 41.0 28-29 37.68106161343106 40.0 37.0 41.0 32.5 41.0 30-31 37.67682285342326 40.0 37.0 41.0 32.5 41.0 32-33 37.51313605038638 40.0 36.5 41.0 32.0 41.0 34-35 37.35858516237424 39.5 36.0 41.0 32.0 41.0 36-37 37.129858338077625 39.0 36.0 41.0 31.0 41.0 38-39 36.9739627914754 39.0 36.0 41.0 31.0 41.0 40-41 36.74358400781912 39.0 35.0 41.0 30.5 41.0 42-43 36.51884263811863 39.0 35.0 40.5 30.0 41.0 44-45 36.26875803709705 39.0 35.0 40.0 30.0 41.0 46-47 35.90820341270568 38.5 35.0 40.0 29.0 41.0 48-49 35.48721131145518 38.0 35.0 40.0 27.5 41.0 50-51 35.29057191285895 38.0 34.0 40.0 27.0 41.0 52-53 35.00441368506125 38.0 34.0 40.0 26.5 41.0 54-55 34.719997243002 38.0 34.0 40.0 26.0 41.0 56-57 34.43574851002671 38.0 33.5 40.0 26.0 41.0 58-59 34.05765460090215 37.0 33.0 40.0 24.5 41.0 60-61 33.681200458637065 37.0 33.0 39.5 23.0 41.0 62-63 33.37122082260062 36.0 32.0 39.0 23.0 40.5 64-65 32.925194781410866 36.0 32.0 39.0 22.0 40.0 66-67 32.52165736387198 35.0 31.0 38.5 22.0 40.0 68-69 32.06413230206347 35.0 31.0 38.0 20.5 40.0 70-71 31.584687364389538 35.0 30.5 37.0 19.5 39.5 72-73 31.158094177857222 34.0 30.0 37.0 18.0 39.0 74-75 30.79494279975635 34.0 30.0 36.0 17.5 39.0 76-77 29.388545867687984 32.5 28.5 35.0 14.5 37.0 78-79 30.187882695209392 34.0 29.5 35.0 16.0 37.0 80-81 30.03727670306833 34.0 30.0 35.0 12.0 37.0 82-83 29.702995423582387 34.0 29.0 35.0 8.5 36.5 84-85 29.261080743214997 34.0 29.0 35.0 7.0 36.0 86-87 28.8681455754661 34.0 29.0 35.0 2.0 36.0 88-89 28.454381138550595 33.0 28.5 35.0 2.0 35.0 90-91 28.001939354006502 33.0 27.0 35.0 2.0 35.0 92-93 27.528631473968765 33.0 26.5 35.0 2.0 35.0 94-95 26.983864594571997 32.5 25.5 34.0 2.0 35.0 96-97 26.28851361976917 32.0 25.0 34.0 2.0 35.0 98-99 25.384249877577346 32.0 22.0 34.0 2.0 35.0 100-101 22.51304000334423 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.46579267210680797 1101 2 0.388888044038044 1101 3 0.21407507614420496 1101 4 0.15886525301792886 1101 5 0.20964792546578792 1101 6 0.12228816878369031 1101 7 0.2028660739975976 1101 8 0.20805431648017247 1101 9 0.21117404590349054 1101 10-11 0.3096836395963791 1101 12-13 0.330188354445518 1101 14-15 0.4683271374213547 1101 16-17 0.394201719148505 1101 18-19 0.3053944588961244 1101 20-21 0.19215866373841806 1101 22-23 0.24098075604300817 1101 24-25 0.25416179632225067 1101 26-27 0.1945756409117365 1101 28-29 0.21573962284587367 1101 30-31 0.061312897429417035 1101 32-33 0.289333814529634 1101 34-35 0.267605946981746 1101 36-37 0.2420849449891378 1101 38-39 0.37661453290309055 1101 40-41 0.460503756366208 1101 42-43 0.46525791633365543 1101 44-45 0.4317301620244365 1101 46-47 0.5362498828723616 1101 48-49 0.5644275482921728 1101 50-51 0.5571150181555353 1101 52-53 0.617662603009002 1101 54-55 0.45938731915722997 1101 56-57 0.49194100451043 1101 58-59 0.4689459052802505 1101 60-61 0.5910826268843508 1101 62-63 0.43622345543575136 1101 64-65 0.4404538044586772 1101 66-67 0.18094249281769947 1101 68-69 0.17764420815168336 1101 70-71 0.279483354927752 1101 72-73 0.3298620506031824 1101 74-75 0.24134949086611712 1101 76-77 0.6200426099281167 1101 78-79 0.7165627603586202 1101 80-81 0.37987409192000854 1101 82-83 0.4343683615461593 1101 84-85 0.5375895097290986 1101 86-87 0.47747270553531607 1101 88-89 0.4284092964109085 1101 90-91 0.08152009978557473 1101 92-93 0.21264816980031398 1101 94-95 0.03439352221755598 1101 96-97 0.0027452745594445105 1101 98-99 0.17390583545284954 1101 100-101 0.2310218789771632 1102 1 0.4201508276382917 1102 2 0.47096252154015517 1102 3 0.1551537849793263 1102 4 0.1348073601932427 1102 5 0.10710228739704775 1102 6 0.06744151789676778 1102 7 0.18454204433883348 1102 8 -0.0881175983091893 1102 9 0.031159689744896468 1102 10-11 0.12425289610509793 1102 12-13 0.1778681273648175 1102 14-15 0.27859971984340604 1102 16-17 0.30226556336027244 1102 18-19 0.14253554444322702 1102 20-21 0.09446693434571785 1102 22-23 0.03159668642864233 1102 24-25 0.06757872148646982 1102 26-27 -0.07963254688124266 1102 28-29 -0.06111828726325541 1102 30-31 0.07888209604364249 1102 32-33 0.22657009038602638 1102 34-35 0.3676286642812059 1102 36-37 0.2505382765660613 1102 38-39 0.25343747557159446 1102 40-41 0.35395081184340427 1102 42-43 0.16033386301365482 1102 44-45 0.2032509750981859 1102 46-47 0.23990564803212067 1102 48-49 0.1762405020613116 1102 50-51 0.14868817960652336 1102 52-53 -0.0012236535911753776 1102 54-55 0.08659234456788312 1102 56-57 0.08542353085872634 1102 58-59 -0.01864893892060593 1102 60-61 0.18663089621629325 1102 62-63 0.15761969044716295 1102 64-65 -0.046116034351278756 1102 66-67 0.14429422373746803 1102 68-69 -0.06128567069777091 1102 70-71 -0.18680085841601013 1102 72-73 -0.07871267064317777 1102 74-75 -0.5404858781442137 1102 76-77 -0.19689214045008185 1102 78-79 -0.017002437661556513 1102 80-81 0.10109402138940027 1102 82-83 0.27836428208134123 1102 84-85 0.1938534851684004 1102 86-87 0.02249795257283793 1102 88-89 0.11359136623167743 1102 90-91 0.16319990878406188 1102 92-93 0.3818124458715104 1102 94-95 -0.005685101034384132 1102 96-97 0.08784704722199521 1102 98-99 -0.31765583100265715 1102 100-101 -0.2938733865110841 1103 1 0.3915971900982633 1103 2 0.43011632541723444 1103 3 0.22384244665177278 1103 4 0.12422518667179361 1103 5 0.06842307174748896 1103 6 0.07237599214378321 1103 7 0.13773382898839515 1103 8 0.1615045915708606 1103 9 0.05324728749255314 1103 10-11 0.1204064287760005 1103 12-13 0.12230134854074493 1103 14-15 0.169418805613752 1103 16-17 0.08509849973891193 1103 18-19 0.2625135851602849 1103 20-21 0.2973019525037941 1103 22-23 0.08526358488318664 1103 24-25 0.08555264914935634 1103 26-27 -0.019665391365954576 1103 28-29 0.14966968267945902 1103 30-31 0.1434909627405645 1103 32-33 0.09567369135120174 1103 34-35 -0.015889719535273628 1103 36-37 0.14781580589215793 1103 38-39 0.2270433249201531 1103 40-41 0.2176608795053454 1103 42-43 0.11804130237771915 1103 44-45 0.2108130286524812 1103 46-47 0.1027787951919592 1103 48-49 0.35546333725192625 1103 50-51 0.09714884331641116 1103 52-53 0.1627506604532556 1103 54-55 0.12100853169644665 1103 56-57 0.04709384474548273 1103 58-59 0.08318754366871417 1103 60-61 0.1847653015633881 1103 62-63 0.003261082792164416 1103 64-65 -0.14592001038577251 1103 66-67 -0.13630110769762638 1103 68-69 -0.10335219196836576 1103 70-71 -0.18738433150982914 1103 72-73 -0.21292790651761706 1103 74-75 -0.0697177364321071 1103 76-77 -0.15483290746056966 1103 78-79 -0.16866232827985428 1103 80-81 0.004673552857632046 1103 82-83 0.2480990276577124 1103 84-85 0.2672770658988419 1103 86-87 0.002782741642800346 1103 88-89 -0.11502230210800946 1103 90-91 -0.21282160540047457 1103 92-93 -0.07719702950980434 1103 94-95 -0.06201581489790442 1103 96-97 -0.5144354576390775 1103 98-99 -0.49945563814036475 1103 100-101 -0.1448514430365293 1104 1 0.3097227338633708 1104 2 0.3523881408917795 1104 3 0.1667357630505677 1104 4 0.04008810524540252 1104 5 0.10985164661897073 1104 6 0.17698836544601448 1104 7 0.1482323616761576 1104 8 0.04641939266630146 1104 9 -0.08440712573117537 1104 10-11 -0.02995571979005973 1104 12-13 -0.0349731942948992 1104 14-15 0.14546522457657574 1104 16-17 -0.009133332380962145 1104 18-19 -0.004705743675913254 1104 20-21 0.008789926809569693 1104 22-23 0.07126798481152008 1104 24-25 0.07353328127052094 1104 26-27 0.23801741212024297 1104 28-29 -0.011076220454640406 1104 30-31 -0.03575711304392115 1104 32-33 0.08327052472028384 1104 34-35 0.18357015049934233 1104 36-37 0.0746474156457495 1104 38-39 0.1196111052659603 1104 40-41 0.21135450969124037 1104 42-43 0.1560080566894868 1104 44-45 0.1976318210139425 1104 46-47 0.28862977603777296 1104 48-49 0.25724198639278484 1104 50-51 0.3042598850719429 1104 52-53 0.22070758582157168 1104 54-55 0.3795605560089257 1104 56-57 0.28035582083307276 1104 58-59 0.16204392782254473 1104 60-61 0.10064529124841215 1104 62-63 -0.033435882084724255 1104 64-65 -0.1419843158566394 1104 66-67 0.024155590725321474 1104 68-69 0.06702001047814576 1104 70-71 -0.03788949582229151 1104 72-73 -0.10495292593851602 1104 74-75 -0.024747106777216032 1104 76-77 -0.005609100771426512 1104 78-79 0.14126349874520017 1104 80-81 0.1538195353623557 1104 82-83 0.14648665114210146 1104 84-85 0.1759048457787138 1104 86-87 0.22967900069952663 1104 88-89 0.1605065408401778 1104 90-91 0.20939246955013857 1104 92-93 0.21034666249360257 1104 94-95 0.288147425094607 1104 96-97 0.08728175558432483 1104 98-99 0.09428303969603036 1104 100-101 0.033496081411310996 1105 1 0.39769754847758776 1105 2 0.42727444026492734 1105 3 0.21757009864862198 1105 4 0.10567484827004137 1105 5 -0.042058762862581034 1105 6 0.019211322219859994 1105 7 0.1482578497326159 1105 8 0.12273979346598907 1105 9 0.06061951725283876 1105 10-11 0.05529983780099457 1105 12-13 0.12155254465961463 1105 14-15 0.18287253168376338 1105 16-17 0.12604795142058123 1105 18-19 -0.029811479285115183 1105 20-21 0.13062960693445547 1105 22-23 0.11597184545556871 1105 24-25 -0.003217827858186695 1105 26-27 0.005449251481771 1105 28-29 0.045294798032173844 1105 30-31 0.07002310025925595 1105 32-33 0.1445284797816484 1105 34-35 0.13708511278498037 1105 36-37 0.23214010774205462 1105 38-39 0.11878772156716622 1105 40-41 0.1329530489070052 1105 42-43 0.15020275358073576 1105 44-45 -0.06390530791579607 1105 46-47 0.35604568536400905 1105 48-49 0.31450090522129415 1105 50-51 0.2611745160128507 1105 52-53 0.20826483438587928 1105 54-55 0.2538032819565643 1105 56-57 0.07697805597632623 1105 58-59 0.23375198603089586 1105 60-61 0.1289868152748923 1105 62-63 0.13386712311643123 1105 64-65 0.1703919276817416 1105 66-67 0.15451286159168376 1105 68-69 -0.04022317744051662 1105 70-71 0.02346642895608042 1105 72-73 0.010511155814427298 1105 74-75 0.1291579422474669 1105 76-77 0.24701537254178163 1105 78-79 0.09871278149536167 1105 80-81 -0.09487081661177399 1105 82-83 0.28140548761232154 1105 84-85 0.24571264990299468 1105 86-87 0.05235002954085388 1105 88-89 0.09607173849208905 1105 90-91 -0.07073262105193479 1105 92-93 0.228505907091332 1105 94-95 0.23168739753564438 1105 96-97 0.2283762684836681 1105 98-99 0.18366686593120463 1105 100-101 0.5471110843185407 1106 1 0.3511983251839901 1106 2 0.35479903596775486 1106 3 0.12766968100648768 1106 4 0.04430984424156037 1106 5 -0.07640138135279528 1106 6 -0.025045948531797535 1106 7 0.010906368592486615 1106 8 0.05792489349817487 1106 9 0.10613891092203431 1106 10-11 -0.10915000016025544 1106 12-13 0.004532978055792114 1106 14-15 0.1276879914399771 1106 16-17 0.13517600101783245 1106 18-19 -0.06684598024375532 1106 20-21 0.011025617242559349 1106 22-23 -0.01887698168776808 1106 24-25 -0.04401210762043917 1106 26-27 -0.013008871347800266 1106 28-29 -0.01931667882957555 1106 30-31 -0.05007420296012555 1106 32-33 0.03684218520100302 1106 34-35 -0.09795124347235173 1106 36-37 0.05811878780099988 1106 38-39 0.16927013921329603 1106 40-41 0.08257978999887428 1106 42-43 -0.04655167324592213 1106 44-45 0.051712188670492765 1106 46-47 0.09147169433261837 1106 48-49 0.02680155557545305 1106 50-51 0.18133759436923214 1106 52-53 0.24297431420250604 1106 54-55 0.06361907158807867 1106 56-57 0.045639901798601556 1106 58-59 0.01358570888365307 1106 60-61 0.2530044366140274 1106 62-63 -0.15710808825366485 1106 64-65 0.1313993668697222 1106 66-67 0.12646926207229825 1106 68-69 0.026968724020733248 1106 70-71 0.19373846612603174 1106 72-73 0.1380731373853905 1106 74-75 0.2160196085376107 1106 76-77 0.2052321665936283 1106 78-79 0.15705012997763745 1106 80-81 0.09733642904936346 1106 82-83 0.21747155463898693 1106 84-85 0.1963936924689058 1106 86-87 0.05149855139959314 1106 88-89 -0.015844257261516503 1106 90-91 -0.003950264782606183 1106 92-93 0.16721157634835393 1106 94-95 0.22760244164896903 1106 96-97 0.09487643142207602 1106 98-99 0.22657204777246776 1106 100-101 0.4499686041760391 1107 1 0.7012583001325119 1107 2 0.6328526438251991 1107 3 0.42740256812234634 1107 4 0.22909854714216493 1107 5 0.0919176120330718 1107 6 0.26832805003613913 1107 7 0.269566548507683 1107 8 0.26972162329535365 1107 9 0.3964420280753558 1107 10-11 0.3439235832633045 1107 12-13 0.43937668613846625 1107 14-15 0.5766682725647101 1107 16-17 0.47887636366602493 1107 18-19 0.45514620130037287 1107 20-21 0.44357949337089764 1107 22-23 0.4844567424718633 1107 24-25 0.4277095594032829 1107 26-27 0.41208010194669953 1107 28-29 0.42186794461652255 1107 30-31 0.3385667203163294 1107 32-33 0.3011600582181657 1107 34-35 0.42847126773616395 1107 36-37 0.4471955306450113 1107 38-39 0.5023146953407789 1107 40-41 0.6259441385348694 1107 42-43 0.4273551228855723 1107 44-45 0.3473260802774689 1107 46-47 0.4529435558044739 1107 48-49 0.3926636990791579 1107 50-51 0.43859502151631347 1107 52-53 0.29769687095916453 1107 54-55 0.5364071877667485 1107 56-57 0.533182072438187 1107 58-59 0.34312971592432007 1107 60-61 0.3463518493439608 1107 62-63 0.3725711881097311 1107 64-65 0.4660687471253695 1107 66-67 0.35260581870929997 1107 68-69 0.43622669928962665 1107 70-71 0.5475045358417212 1107 72-73 0.6671077916150949 1107 74-75 0.7598882918679521 1107 76-77 0.7067197190174248 1107 78-79 0.8491100113208461 1107 80-81 0.7718805374153632 1107 82-83 0.6643457162924626 1107 84-85 0.8840228822715268 1107 86-87 1.0593667049431765 1107 88-89 1.1491034974655499 1107 90-91 1.1122777355570754 1107 92-93 1.274962626754327 1107 94-95 1.3179593094802016 1107 96-97 1.5343466060683575 1107 98-99 1.5226497440396436 1107 100-101 1.6039168906198675 1108 1 0.32933855321276084 1108 2 0.271630532603087 1108 3 0.004991645711420745 1108 4 0.11375555679917682 1108 5 -0.01143736632190695 1108 6 0.011002042710131832 1108 7 -0.06663891269777622 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-1.2536909192662051 2316 62-63 -1.402843268013335 2316 64-65 -1.4278445751580158 2316 66-67 -1.4766782258809954 2316 68-69 -1.5260288928032253 2316 70-71 -1.4383965227784543 2316 72-73 -1.498947716604686 2316 74-75 -1.7887449154338881 2316 76-77 -1.5851691114413669 2316 78-79 -1.3467919958638355 2316 80-81 -1.505083240733704 2316 82-83 -1.311656711145126 2316 84-85 -1.4815413971189955 2316 86-87 -1.4017227352595363 2316 88-89 -1.404019165792942 2316 90-91 -1.3851945619216295 2316 92-93 -1.7001181399076764 2316 94-95 -2.1443385797141516 2316 96-97 -2.25935004411215 2316 98-99 -2.513980655289373 2316 100-101 -3.055973225670815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 8.0 4 30.0 5 59.0 6 142.0 7 367.0 8 841.0 9 1745.0 10 2776.0 11 4144.0 12 5764.0 13 7847.0 14 9821.0 15 12388.0 16 15089.0 17 18756.0 18 16939.0 19 12675.0 20 11962.0 21 12631.0 22 13459.0 23 15250.0 24 17979.0 25 21885.0 26 27037.0 27 33248.0 28 41683.0 29 51029.0 30 62860.0 31 77274.0 32 96478.0 33 121258.0 34 158892.0 35 212298.0 36 297183.0 37 382354.0 38 231726.0 39 13554.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89809019709617 18.878893589797755 14.955830904083353 23.26718530902273 2 15.995851025124292 20.018554916430706 39.28724832281573 24.69834573562927 3 19.806543538403883 23.66119041018921 29.48235267184614 27.049913379560763 4 13.70954857455993 16.631254015963815 35.73845704368323 33.92074036579302 5 14.233574462833278 36.60437377328519 33.83309313278578 15.328958631095752 6 33.11025205132594 35.29047014280653 16.885916020049446 14.713361785818083 7 29.318235202783672 31.947734484172642 20.01774630840954 18.716284004634147 8 27.073620804286985 34.41962703888462 19.12102524494484 19.38572691188356 9 26.264518434392954 16.678295680874637 19.84490445130794 37.21228143342448 10-11 25.554380814625468 26.13014210771083 26.663851601439514 21.651625476224186 12-13 26.26437911342797 23.306717095686576 27.435112034537244 22.99379175634821 14-15 23.062916053620143 24.929264819427782 25.580891908980224 26.42692721797185 16-17 22.653093013349046 27.948843255208438 26.477581724586845 22.920482006855668 18-19 22.553587282376313 26.37601073338137 28.907422595041783 22.16297938920053 20-21 23.164008535745424 25.62460436581084 29.054080954219895 22.15730614422384 22-23 22.775322800056436 25.470492699472157 29.101335491844743 22.652849008626664 24-25 22.764393122036473 25.640081376229702 29.18829798669475 22.40722751503908 26-27 22.487485263241187 25.908204859982753 28.94483562759806 22.659474249178004 28-29 22.49317219990525 26.117753673674947 28.973958810250856 22.415115316168947 30-31 22.656159217171624 25.778037220975186 28.904369220846792 22.661434341006398 32-33 22.484726946342317 25.818260179526654 28.90452310323865 22.792489770892374 34-35 22.92602258159552 25.909141127009534 28.721986997268868 22.442849294126077 36-37 23.056440162498937 25.737607913337886 28.606869725639967 22.599082198523217 38-39 22.731482426102144 25.903278991196004 28.712783581803585 22.652455000898264 40-41 22.974076933853468 25.814075649855837 28.6062977097812 22.605549706509496 42-43 22.880130438580316 25.844611955426515 28.346600909854885 22.92865669613828 44-45 22.91945618173127 25.95899391082195 28.369607708231054 22.75194219921573 46-47 23.184477509139573 26.256995890303248 27.7986298356619 22.759896764895288 48-49 23.264302085121948 26.30351850331096 27.57457440269555 22.857605008871534 50-51 23.192905616320726 26.505328277480995 27.566654167208902 22.735111938989373 52-53 23.45650534865145 26.46847169656387 27.297730400097542 22.777292554687133 54-55 23.075320851403117 26.543947677325257 27.43668017187726 22.94405129939437 56-57 23.11273651680934 26.73648047908161 27.274168267313048 22.876614736796004 58-59 23.28750046033596 26.43191855089226 27.298892904456153 22.981688084315632 60-61 23.071192046800274 26.536620009560526 27.59504594749079 22.79714199614842 62-63 22.9701836488955 26.556267808926314 27.65682244088326 22.816726101294922 64-65 22.91493183706198 26.50754100253698 27.55033017550793 23.02719698489311 66-67 22.886238177804234 26.66311992499089 27.365086127097438 23.085555770107437 68-69 23.059612341742625 26.455037250715318 27.522556831570434 22.962793575971627 70-71 23.04026845804584 26.473430023815546 27.25612576680171 23.230175751336905 72-73 23.050449841161544 26.548006239575724 27.25778410460869 23.143759814654043 74-75 23.03670888091759 26.817702714000774 26.9880244765685 23.15756392851313 76-77 23.13871704438175 26.63654003395031 27.071120982459 23.153621939208932 78-79 23.07837600014334 26.69561499732233 27.0741464813071 23.151862521227226 80-81 23.23123350017321 26.768642066743915 26.903602188429517 23.096522244653357 82-83 23.170435810495533 26.80536286434193 26.988197965595546 23.03600335956699 84-85 23.11142488065877 26.826649320309297 26.942089859473107 23.11983593955883 86-87 23.15091087631562 26.851030176401874 26.848043941451877 23.150015005830625 88-89 23.036653514968148 26.878149164752653 26.68562761279249 23.399569707486712 90-91 22.973003579230262 26.789621352232746 26.899156102076134 23.338218966460865 92-93 22.943634074361615 26.617870277748747 27.138381681820672 23.300113966068967 94-95 22.834005719512934 26.562240265331248 27.157608116811048 23.446145898344763 96-97 22.98109905774005 26.451987147199997 27.09373127375309 23.47318252130686 98-99 22.725661972644136 26.52661079761221 26.900785478160206 23.846941751583447 100-101 22.539247315574197 26.559863665909806 27.04670358384656 23.854185434669432 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 251.0 1 203.5 2 228.5 3 307.0 4 245.5 5 211.0 6 381.0 7 755.5 8 1200.0 9 1481.0 10 1440.5 11 1337.5 12 1426.5 13 1533.0 14 1657.5 15 1872.5 16 2131.0 17 2309.0 18 2429.5 19 2711.0 20 3081.5 21 3558.5 22 4129.5 23 4944.0 24 6018.5 25 7490.0 26 9406.5 27 11802.0 28 14368.0 29 17208.0 30 21047.5 31 25379.5 32 30299.0 33 33471.0 34 35429.5 35 38316.5 36 42129.0 37 46246.0 38 49249.0 39 51273.0 40 53317.5 41 56960.0 42 60229.0 43 63077.0 44 66635.0 45 70016.5 46 72313.5 47 73989.0 48 76755.0 49 77285.0 50 75472.0 51 74462.0 52 74158.5 53 74161.0 54 73291.5 55 69593.0 56 63776.0 57 58286.0 58 54689.5 59 49118.5 60 40409.0 61 33417.0 62 28284.0 63 22146.0 64 15828.5 65 11803.0 66 9304.0 67 7462.0 68 5837.0 69 4551.0 70 3445.0 71 2511.5 72 1921.0 73 1556.5 74 1269.0 75 1012.5 76 752.0 77 507.5 78 343.5 79 233.5 80 161.5 81 100.5 82 65.5 83 37.5 84 24.0 85 12.0 86 5.5 87 4.0 88 3.5 89 2.5 90 1.5 91 1.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0532329533918041 2 0.012988745078211156 3 0.004528642919989331 4 0.013088275691837296 5 0.0 6 0.0 7 0.0 8 0.0 9 0.004478877613176261 10-11 2.737091874718826E-4 12-13 3.4835714769148696E-4 14-15 2.488265340653478E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0011197194032940652 24-25 0.0 26-27 4.976530681306956E-5 28-29 0.0 30-31 2.488265340653478E-5 32-33 0.004603290880208935 34-35 3.981224545045565E-4 36-37 0.0032347449428495214 38-39 1.4929592043920868E-4 40-41 0.0041056378120782385 42-43 0.010475597084151142 44-45 0.0023638520736208044 46-47 0.0018910816588966436 48-49 0.009803765442174704 50-51 5.723010283503E-4 52-53 0.0023389694202142694 54-55 0.010326301163711934 56-57 0.004354464346143587 58-59 0.0014929592043920869 60-61 0.00634507661866637 62-63 0.004055872505265169 64-65 0.017368092077761278 66-67 0.003782163317793287 68-69 3.732398010980217E-4 70-71 0.0015178418577986217 72-73 2.488265340653478E-5 74-75 0.0 76-77 0.001244132670326739 78-79 0.010948367498875304 80-81 0.01592489818018226 82-83 0.012167617515795507 84-85 0.0085845154252545 86-87 0.010600010351183816 88-89 0.028167163656197376 90-91 0.023912229923679924 92-93 0.0030854490224103133 94-95 0.008460102158221825 96-97 0.010948367498875304 98-99 0.010450714430744608 100-101 0.012441326703267391 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2009432.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.70170972283685 #Duplication Level Percentage of deduplicated Percentage of total 1 81.62842178615513 51.99870029754946 2 11.025305089422051 14.046615688241584 3 3.116993894768033 5.956735208771037 4 1.3463404219456188 3.4305674698760593 5 0.7340199424496034 2.3379162652349024 6 0.447743231426962 1.7113205615235174 7 0.32061826285271283 1.4296752058458602 8 0.22357253786025613 1.139356232701759 9 0.16904652358111527 0.969169731733701 >10 0.9148366721148253 10.456996855491687 >50 0.049297284043108895 2.115143315298044 >100 0.02137808433404354 2.543391165032843 >500 0.0017218683556820661 0.7568331114047204 >1k 5.478672040806532E-4 0.5362147637126298 >5k 1.565334868801866E-4 0.5713641275822164 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6016 0.2993880857874265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5444 0.27092233029035073 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3179 0.15820391035874815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.953061362613912E-5 2 4.976530681306956E-5 0.0 0.0 0.0 9.953061362613912E-5 3 4.976530681306956E-5 0.0 0.0 0.0 9.953061362613912E-5 4 4.976530681306956E-5 0.0 0.0 0.0 9.953061362613912E-5 5 4.976530681306956E-5 0.0 0.0 0.0 9.953061362613912E-5 6 4.976530681306956E-5 0.0 0.0 0.0 1.4929592043920868E-4 7 9.953061362613912E-5 0.0 0.0 0.0 1.4929592043920868E-4 8 9.953061362613912E-5 0.0 0.0 0.0 1.4929592043920868E-4 9 9.953061362613912E-5 0.0 0.0 0.0 1.4929592043920868E-4 10-11 9.953061362613912E-5 0.0 0.0 4.976530681306956E-5 1.4929592043920868E-4 12-13 9.953061362613912E-5 0.0 0.0 1.4929592043920868E-4 2.4882653406534783E-4 14-15 9.953061362613912E-5 0.0 0.0 1.4929592043920868E-4 2.985918408784174E-4 16-17 9.953061362613912E-5 0.0 0.0 1.9906122725227824E-4 3.4835714769148696E-4 18-19 9.953061362613912E-5 0.0 0.0 2.2394388065881304E-4 3.4835714769148696E-4 20-21 9.953061362613912E-5 0.0 0.0 2.985918408784174E-4 3.4835714769148696E-4 22-23 9.953061362613912E-5 0.0 0.0 4.2300510791109126E-4 3.4835714769148696E-4 24-25 1.244132670326739E-4 0.0 0.0 4.976530681306957E-4 3.4835714769148696E-4 26-27 1.4929592043920868E-4 0.0 0.0 5.474183749437652E-4 3.732398010980217E-4 28-29 1.9906122725227824E-4 0.0 0.0 9.206581760417869E-4 3.981224545045565E-4 30-31 1.9906122725227824E-4 0.0 0.0 0.0019159643123031781 3.981224545045565E-4 32-33 1.9906122725227824E-4 0.0 0.0 0.003309392903069126 4.976530681306957E-4 34-35 2.488265340653478E-4 0.0 0.0 0.004752586800648144 4.976530681306957E-4 36-37 2.9859184087841737E-4 0.0 0.0 0.006718316419764392 4.976530681306957E-4 38-39 2.9859184087841737E-4 0.0 0.0 0.010077474629646588 4.976530681306957E-4 40-41 2.9859184087841737E-4 0.0 0.0 0.017293444117541672 4.976530681306957E-4 42-43 3.4835714769148696E-4 0.0 0.0 0.023439459508955762 4.976530681306957E-4 44-45 3.4835714769148696E-4 0.0 0.0 0.031202847371794615 4.976530681306957E-4 46-47 3.4835714769148696E-4 0.0 0.0 0.045784082268023996 5.474183749437652E-4 48-49 3.4835714769148696E-4 0.0 0.0 0.05571226097723138 6.220663351633696E-4 50-51 3.4835714769148696E-4 0.0 0.0 0.06700898562379817 6.469489885699044E-4 52-53 3.4835714769148696E-4 0.0 0.0 0.07979866947475706 6.469489885699044E-4 54-55 3.4835714769148696E-4 0.0 0.0 0.09679352175142031 6.967142953829739E-4 56-57 3.4835714769148696E-4 0.0 0.0 0.1377752519119831 7.215969487895087E-4 58-59 3.4835714769148696E-4 0.0 0.0 0.17228749218684683 7.464796021960434E-4 60-61 3.4835714769148696E-4 0.0 0.0 0.19754338539447963 7.464796021960434E-4 62-63 3.4835714769148696E-4 0.0 0.0 0.22717862560166258 8.460102158221826E-4 64-65 3.4835714769148696E-4 0.0 0.0 0.2588293607347748 8.460102158221826E-4 66-67 3.4835714769148696E-4 0.0 0.0 0.3138200247632167 8.708928692287174E-4 68-69 3.4835714769148696E-4 0.0 0.0 0.382048260403935 9.206581760417869E-4 70-71 3.4835714769148696E-4 0.0 0.0 0.4169337404798968 9.704234828548566E-4 72-73 3.4835714769148696E-4 0.0 0.0 0.45497931753848847 0.0010699540964809956 74-75 3.4835714769148696E-4 0.0 0.0 0.5296521604114994 0.0011446020567006 76-77 3.4835714769148696E-4 0.0 0.0 0.6191301820613984 0.0012690153237332738 78-79 3.4835714769148696E-4 0.0 0.0 0.708210081256793 0.0013436632839528783 80-81 4.478877613176261E-4 0.0 0.0 0.7829078067832104 0.001368545937359413 82-83 4.478877613176261E-4 0.0 0.0 0.9018717727198532 0.0014431938975790174 84-85 4.478877613176261E-4 0.0 0.0 1.1051381683978359 0.0014431938975790174 86-87 4.478877613176261E-4 0.0 0.0 1.2864829464246612 0.0015178418577986217 88-89 4.478877613176261E-4 0.0 0.0 1.5266751997579415 0.001592489818018226 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4730 0.0 41.116474 1 GTATCAA 7410 0.0 35.577553 1 TCAACGC 9260 0.0 28.211384 4 ATCAACG 9305 0.0 28.07565 3 TATCAAC 9495 0.0 27.671492 2 CAACGCA 9455 0.0 27.624052 5 AACGCAG 9790 0.0 26.678795 6 TACCTGG 1750 0.0 23.620201 2 ACGCAGA 11345 0.0 22.812778 7 GTACATG 6690 0.0 22.75386 1 CGCAGAG 11455 0.0 22.510801 8 GTACCTG 1915 0.0 22.066622 1 ACATGGG 6625 0.0 21.508932 3 TACATGG 6880 0.0 21.338919 2 GAGTACT 7425 0.0 21.10589 12-13 GCAGAGT 12475 0.0 20.55604 9 TAAGGTG 940 0.0 20.20777 5 GTACTTT 7870 0.0 20.002996 14-15 GTGTAGC 1035 0.0 19.486368 1 AGAGTAC 10655 0.0 19.38751 10-11 >>END_MODULE