FastQCFastQC Report
Thu 26 May 2016
SRR936437_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936437_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996159
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51040.2556910546704947No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41340.2070977311927557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.11612301424886494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40800.038.7943041
GTATCAA63850.034.690371
TCAACGC80700.026.9183444
ATCAACG80750.026.8421383
TATCAAC82700.026.3241752
CAACGCA83250.026.034765
AACGCAG85400.025.4893536
GTACCTG17300.023.9032551
TACCTGG16650.023.6951052
GTACATG70500.022.9905381
TACATGG69500.022.5696032
ACGCAGA97000.022.3442657
GTGGTAT25750.022.1507231
CGCAGAG98250.022.0088528
ACATGGG69750.021.8753813
GTATAGC7250.021.6351451
TATAACG1100.002866542721.6058862
TGGTATC26900.020.6741852
GGACCGT3102.582965E-1019.9311736
GAGTACT58750.019.61912-13
Produced by FastQC (version 0.11.5)