FastQCFastQC Report
Thu 26 May 2016
SRR936437_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936437_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1996159
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61160.30638841895861No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55250.27678155898402884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29900.1497876672148862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.045.8835331
GTATCAA73550.037.0257071
ATCAACG92800.029.0212573
TATCAAC93400.028.9895672
TCAACGC93100.028.9773064
CAACGCA96600.027.8782375
AACGCAG98750.027.3681476
TACCTGG16950.026.6236532
GTATACG1151.2774576E-424.7961831
GTACCTG18800.024.77421
ACGCAGA112900.023.895977
CGCAGAG114450.023.4478458
GTACATG66600.023.1921251
TACATGG66550.022.2700042
ACATGGG65700.021.5442833
GAGTACT77450.021.4344512-13
GTATAGC7250.020.9770011
GTGTAGC12250.020.9502431
GCAGAGT127050.020.9360379
GTACTTT81300.020.09782414-15
Produced by FastQC (version 0.11.5)