##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936437_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1996159 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.862062090244315 31.0 31.0 34.0 30.0 34.0 2 32.00609420391863 31.0 31.0 34.0 30.0 34.0 3 32.11757730721851 33.0 31.0 34.0 30.0 34.0 4 35.86663687612059 37.0 35.0 37.0 35.0 37.0 5 35.634216512812856 37.0 35.0 37.0 33.0 37.0 6 35.72661847077312 37.0 35.0 37.0 33.0 37.0 7 35.67618862024518 37.0 35.0 37.0 33.0 37.0 8 35.64738881021001 37.0 35.0 37.0 33.0 37.0 9 37.286263268607364 39.0 37.0 39.0 34.0 39.0 10-11 37.15332420914366 39.0 37.0 39.0 33.0 39.0 12-13 37.053580651641475 39.0 37.0 39.0 33.0 39.0 14-15 38.16951029452063 40.0 37.5 41.0 33.0 41.0 16-17 38.08273464187973 40.0 37.0 41.0 33.0 41.0 18-19 38.214672027629064 40.0 37.5 41.0 33.0 41.0 20-21 38.23731952214227 40.0 38.0 41.0 33.0 41.0 22-23 38.14849969366168 40.0 37.5 41.0 33.0 41.0 24-25 38.02650164641193 40.0 37.0 41.0 33.0 41.0 26-27 37.81110973624847 40.0 37.0 41.0 33.0 41.0 28-29 37.72697941396452 40.0 37.0 41.0 32.5 41.0 30-31 37.70087302664768 40.0 37.0 41.0 33.0 41.0 32-33 37.56371361199183 40.0 36.5 41.0 32.0 41.0 34-35 37.398597005549156 40.0 36.0 41.0 32.0 41.0 36-37 37.14451228584497 39.0 36.0 41.0 31.0 41.0 38-39 36.97541278024446 39.0 36.0 41.0 31.0 41.0 40-41 36.759074803159464 39.0 35.0 41.0 30.5 41.0 42-43 36.5214133743855 39.0 35.0 40.5 30.0 41.0 44-45 36.29746152485849 39.0 35.0 40.0 30.0 41.0 46-47 35.96864277845602 39.0 35.0 40.0 29.5 41.0 48-49 35.62349867921343 38.0 35.0 40.0 28.0 41.0 50-51 35.29951496849699 38.0 34.5 40.0 27.0 41.0 52-53 35.01842563643477 38.0 34.0 40.0 26.5 41.0 54-55 34.74286467160181 38.0 34.0 40.0 26.0 41.0 56-57 34.419818010489145 38.0 33.5 40.0 25.5 41.0 58-59 34.06442097047379 37.0 33.0 40.0 25.0 41.0 60-61 33.69739409536014 37.0 33.0 39.5 24.0 41.0 62-63 33.280004749120685 36.0 32.0 39.0 23.0 40.0 64-65 32.8494185583413 35.5 31.5 39.0 22.0 40.0 66-67 32.38026003940568 35.0 31.0 38.5 21.5 40.0 68-69 32.084345986467014 35.0 31.0 38.0 20.5 40.0 70-71 31.647116036347803 35.0 31.0 37.5 19.5 39.5 72-73 31.186390963846065 34.5 30.0 37.0 18.0 39.0 74-75 30.664603120292522 34.0 30.0 36.0 16.5 39.0 76-77 29.26821986625314 32.5 28.0 35.0 13.0 37.0 78-79 30.049873532118433 34.0 29.0 35.0 13.5 37.0 80-81 29.864372527438945 34.0 29.0 35.0 10.0 37.0 82-83 29.529929980527605 34.0 29.0 35.0 7.0 36.5 84-85 29.11418905007066 34.0 29.0 35.0 6.0 36.0 86-87 28.718073309791453 33.5 29.0 35.0 2.0 36.0 88-89 28.308722902333933 33.0 27.5 35.0 2.0 35.0 90-91 27.82285980225022 33.0 27.0 35.0 2.0 35.0 92-93 27.302618679173353 33.0 26.0 34.5 2.0 35.0 94-95 26.687529901175207 32.0 25.0 34.0 2.0 35.0 96-97 25.896075913792437 32.0 24.5 34.0 2.0 35.0 98-99 24.888356588828845 32.0 19.0 34.0 2.0 35.0 100-101 21.57578028603934 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.27154928486845975 1101 2 0.3055932054692363 1101 3 0.263586264361507 1101 4 0.16378186531371597 1101 5 0.08328109299714015 1101 6 0.12307305186600814 1101 7 0.07184105838606314 1101 8 0.1265145648423669 1101 9 0.14591230974749436 1101 10-11 0.1418943336639913 1101 12-13 0.18993284159303414 1101 14-15 0.398257846471715 1101 16-17 0.44827956325413965 1101 18-19 0.3017983117918277 1101 20-21 0.3240837627075308 1101 22-23 0.3534005516400214 1101 24-25 0.09916389994547359 1101 26-27 0.1906990738919916 1101 28-29 0.09663662970167053 1101 30-31 0.16417073950918848 1101 32-33 0.29857675942334083 1101 34-35 0.19815795193112962 1101 36-37 0.15982988265410114 1101 38-39 0.229174753886781 1101 40-41 0.10567048845585703 1101 42-43 0.26216649286290306 1101 44-45 0.25935597429376855 1101 46-47 0.35212689274898423 1101 48-49 0.2838042727812322 1101 50-51 0.33318564920588756 1101 52-53 0.4597098426580075 1101 54-55 0.35940332679433595 1101 56-57 0.2598543318408062 1101 58-59 0.24386676270201946 1101 60-61 0.2142432125728888 1101 62-63 0.20213243775734213 1101 64-65 0.17671921018387593 1101 66-67 0.1225323113966823 1101 68-69 0.08569241173440645 1101 70-71 0.26130030496622325 1101 72-73 0.32691634478369025 1101 74-75 0.07878192935840644 1101 76-77 -0.07008260813825373 1101 78-79 -0.1188612049998099 1101 80-81 0.09188275653111333 1101 82-83 0.27491657288212323 1101 84-85 0.28958317034948067 1101 86-87 0.2941315101397528 1101 88-89 -0.09103727883581314 1101 90-91 -0.11233370169365386 1101 92-93 -0.07794047370000357 1101 94-95 0.0405920234032493 1101 96-97 0.13991198571001817 1101 98-99 0.34640696657679015 1101 100-101 0.7731486262583118 1102 1 0.21281144511229044 1102 2 0.32899014087903566 1102 3 0.21320882709375155 1102 4 0.17990937564731269 1102 5 0.16161560930125773 1102 6 0.20620862031826448 1102 7 0.20084694540539516 1102 8 0.12038413187505626 1102 9 0.26303518561906714 1102 10-11 0.2534777748564281 1102 12-13 0.25618811277168874 1102 14-15 0.42394069896221254 1102 16-17 0.24072079744060204 1102 18-19 0.28741937145530017 1102 20-21 0.17599746150925455 1102 22-23 0.22957858178489943 1102 24-25 0.215538581587154 1102 26-27 -0.04918235068318211 1102 28-29 0.029271592751229036 1102 30-31 0.22930872957223158 1102 32-33 0.2380475539621898 1102 34-35 0.33125949257661347 1102 36-37 0.3873516980271532 1102 38-39 0.38087969313775005 1102 40-41 0.3277524474554738 1102 42-43 0.3919337259638169 1102 44-45 0.3483674978209663 1102 46-47 0.4142410007194286 1102 48-49 0.24145698039260566 1102 50-51 0.21007534065927302 1102 52-53 0.10826126013535742 1102 54-55 0.2503640382468859 1102 56-57 0.2158405119585467 1102 58-59 -0.02563346693349189 1102 60-61 0.02027740738729733 1102 62-63 0.07188985915936996 1102 64-65 -0.060865650573504126 1102 66-67 0.1219546737197561 1102 68-69 -0.031078704294152715 1102 70-71 0.09042393237217539 1102 72-73 0.05453523334784194 1102 74-75 -0.05657588451157736 1102 76-77 -0.36125938572582683 1102 78-79 -0.2018537731598009 1102 80-81 0.22850899343145414 1102 82-83 0.06958923790654481 1102 84-85 0.006817106302051457 1102 86-87 -0.11822392387135139 1102 88-89 0.013001800952086029 1102 90-91 0.15235295022067064 1102 92-93 0.13624640784982844 1102 94-95 0.24944530719098523 1102 96-97 0.4172953935009218 1102 98-99 0.3362424640508017 1102 100-101 0.7867816687200033 1103 1 0.10959237561456092 1103 2 0.1346818289764684 1103 3 0.19043050089693736 1103 4 0.15366368002968755 1103 5 -0.06474479030133296 1103 6 0.1774689880232856 1103 7 0.1724254933738507 1103 8 0.0878742623210087 1103 9 0.09480989967444486 1103 10-11 -0.0058253188898191866 1103 12-13 0.04642200708334343 1103 14-15 -0.012349669164400723 1103 16-17 0.33389650692293316 1103 18-19 0.11280860534881754 1103 20-21 0.27672010854631424 1103 22-23 0.1744208399420515 1103 24-25 0.11344288728202656 1103 26-27 0.1440648300408185 1103 28-29 0.2237763809664699 1103 30-31 0.10389181399580139 1103 32-33 0.20983620033585026 1103 34-35 0.19697857201747837 1103 36-37 0.21616624188790468 1103 38-39 0.27809861865890895 1103 40-41 0.1452678135735752 1103 42-43 0.3053028850690609 1103 44-45 0.302089073386 1103 46-47 0.17170958638429568 1103 48-49 0.12573521630577034 1103 50-51 0.17972004864535762 1103 52-53 0.121406579425269 1103 54-55 0.1538943675873199 1103 56-57 -0.01065859435264116 1103 58-59 0.15526334800252783 1103 60-61 0.1242771122751023 1103 62-63 0.06502730746502294 1103 64-65 0.09855906757843513 1103 66-67 0.1856850867416 1103 68-69 -0.015828025973604554 1103 70-71 -0.07421566086617304 1103 72-73 -0.09604735471451065 1103 74-75 -0.046655521054262294 1103 76-77 -0.20750623076965624 1103 78-79 -0.22731068001809618 1103 80-81 -0.09053463226446823 1103 82-83 0.049278300221356375 1103 84-85 -0.1876687899600462 1103 86-87 -0.16693483642030316 1103 88-89 -0.16141944903498384 1103 90-91 -0.26085602500820215 1103 92-93 -0.024540817848954788 1103 94-95 0.025469768094186662 1103 96-97 0.1382851091193551 1103 98-99 0.25080310881128653 1103 100-101 0.19800669057500997 1104 1 0.1428618519136151 1104 2 0.2314875119440316 1104 3 0.1820010638838383 1104 4 0.08983768736322162 1104 5 0.07055172266499454 1104 6 0.18451697681591384 1104 7 0.10045522237092541 1104 8 0.09601159967548512 1104 9 0.12689111841833522 1104 10-11 0.15737027836818385 1104 12-13 0.17651692886811787 1104 14-15 0.20982624771040292 1104 16-17 0.16273439758935382 1104 18-19 0.12269960108281452 1104 20-21 0.1367605089175825 1104 22-23 0.10715771155663134 1104 24-25 0.13219626378054983 1104 26-27 0.2416270800449638 1104 28-29 0.20598915059952105 1104 30-31 0.10849725194670157 1104 32-33 0.042215919866187335 1104 34-35 0.04594328599311126 1104 36-37 0.19431892266867123 1104 38-39 0.16087006021989225 1104 40-41 0.23462428042865469 1104 42-43 0.2255625703418005 1104 44-45 0.1947402315852429 1104 46-47 0.22403755318171648 1104 48-49 0.1664460037100497 1104 50-51 0.05501798065689911 1104 52-53 0.06111875232667785 1104 54-55 0.10147530040131869 1104 56-57 0.19005956591694684 1104 58-59 0.10633867963687038 1104 60-61 0.18936617085815044 1104 62-63 0.09260180444626798 1104 64-65 0.16157682145352936 1104 66-67 -0.009452427607918423 1104 68-69 0.15325631968267572 1104 70-71 0.05225867118963379 1104 72-73 0.03153107548986611 1104 74-75 0.2115706030281288 1104 76-77 0.03125525896559722 1104 78-79 0.022765285173647953 1104 80-81 0.14808369971730428 1104 82-83 0.16578345409329032 1104 84-85 0.05136368728028273 1104 86-87 0.05240665333808536 1104 88-89 0.01999718996415467 1104 90-91 0.049896751480996926 1104 92-93 0.14093903730046264 1104 94-95 0.2926178497031664 1104 96-97 0.28071733717843017 1104 98-99 0.2751539052696934 1104 100-101 0.7891291553331392 1105 1 0.15528264582562556 1105 2 0.16257564707073158 1105 3 0.08959341202815096 1105 4 0.0655553153577415 1105 5 0.04606039584066224 1105 6 0.01300209314636902 1105 7 0.11187524287873174 1105 8 0.06866197251833484 1105 9 0.05092189176438211 1105 10-11 0.10185807738388775 1105 12-13 0.041826455308267896 1105 14-15 0.14995981984924356 1105 16-17 0.25969873764684337 1105 18-19 0.15433987702723329 1105 20-21 0.19254960411220168 1105 22-23 0.163364813306778 1105 24-25 0.1076235259878402 1105 26-27 0.13353327910253654 1105 28-29 0.10851211527684512 1105 30-31 0.2651336027194162 1105 32-33 0.18303731777005083 1105 34-35 0.14481874853340315 1105 36-37 0.09848425671531658 1105 38-39 0.18384249097695005 1105 40-41 0.09586161084669698 1105 42-43 0.1078551032114845 1105 44-45 0.28341615971874035 1105 46-47 0.25651597442920604 1105 48-49 0.24405081548594865 1105 50-51 0.26165045471684323 1105 52-53 0.2600832823430892 1105 54-55 0.37016367278283724 1105 56-57 0.1642232135935373 1105 58-59 0.2955604489298125 1105 60-61 0.33191037992151706 1105 62-63 0.31016941916305285 1105 64-65 0.1996207484860406 1105 66-67 0.5602585246871925 1105 68-69 0.518213259095468 1105 70-71 0.4478533686218924 1105 72-73 0.36935432064736773 1105 74-75 0.12403442845199919 1105 76-77 0.10046175401018687 1105 78-79 0.21327170016412111 1105 80-81 0.3779846884728997 1105 82-83 0.31676978674238043 1105 84-85 0.4287627425253788 1105 86-87 0.4105374768875656 1105 88-89 0.5370188666036633 1105 90-91 0.3735584617703722 1105 92-93 0.41131368282353975 1105 94-95 0.3531206399671909 1105 96-97 0.3881986175015939 1105 98-99 0.7348489824259694 1105 100-101 0.9945812672693428 1106 1 0.011273819323104561 1106 2 0.10471274550184972 1106 3 0.03538151022093672 1106 4 0.014576510469467507 1106 5 -0.18277099659352558 1106 6 -0.17959235618342007 1106 7 -0.007165635169243956 1106 8 -0.0759753519172719 1106 9 0.03353824298357466 1106 10-11 0.02783400585141038 1106 12-13 -0.04431885324949292 1106 14-15 0.14054652749805285 1106 16-17 0.15978883125379895 1106 18-19 -0.07458931371233746 1106 20-21 0.04645074974949637 1106 22-23 0.031103289132509815 1106 24-25 -0.025327003684495253 1106 26-27 -0.02626329631310398 1106 28-29 -0.02101054270028868 1106 30-31 0.012733126877250811 1106 32-33 -0.10356903994162536 1106 34-35 -0.10967093333446343 1106 36-37 -0.23013407512501516 1106 38-39 -0.010660052600826475 1106 40-41 0.008271106322503385 1106 42-43 -0.012887999542478212 1106 44-45 -0.05192159371446792 1106 46-47 -0.1720119556162416 1106 48-49 -0.2612939851780922 1106 50-51 -0.167927368901033 1106 52-53 -0.2647289284738932 1106 54-55 -0.3084869160615824 1106 56-57 -0.40033188630044236 1106 58-59 -0.42185987421550664 1106 60-61 -0.528641666857304 1106 62-63 -0.6755123747372949 1106 64-65 -0.5664690246054533 1106 66-67 -0.45472290185313113 1106 68-69 -0.46964033519771675 1106 70-71 -0.39246203091294873 1106 72-73 -0.4550732716241015 1106 74-75 -0.2655705978877947 1106 76-77 -0.5315935924341453 1106 78-79 -0.38401953332727956 1106 80-81 -0.5174448844197137 1106 82-83 -0.2907379795936329 1106 84-85 -0.4161012027733193 1106 86-87 -0.35274912145726134 1106 88-89 -0.4051553171092195 1106 90-91 -0.4387009461800524 1106 92-93 -0.3415607860659158 1106 94-95 -0.19413422734762875 1106 96-97 -0.5028984493710773 1106 98-99 -0.1593565707097433 1106 100-101 0.11213965861049857 1107 1 0.05005557810843442 1107 2 0.1469481275330864 1107 3 0.12929394128073568 1107 4 0.07141002235297833 1107 5 0.026688194772582108 1107 6 0.02533695117416812 1107 7 -0.015760842089058258 1107 8 -0.005550534765873749 1107 9 -0.008095358836314404 1107 10-11 0.053603094187458566 1107 12-13 0.014596090738656642 1107 14-15 0.006882711021184207 1107 16-17 0.055788315442185876 1107 18-19 0.03587620626544208 1107 20-21 0.010401800550326357 1107 22-23 -0.11313962507082209 1107 24-25 0.038617930119677624 1107 26-27 0.07760413349022599 1107 28-29 0.002405155166371742 1107 30-31 -0.076992467959343 1107 32-33 0.06754650185894917 1107 34-35 0.13061544130348324 1107 36-37 0.2596100360257694 1107 38-39 0.22009290008999471 1107 40-41 0.16836282820745652 1107 42-43 0.0753612498854963 1107 44-45 0.22715563254166682 1107 46-47 0.2541005111535881 1107 48-49 0.20300011340805213 1107 50-51 0.15387790560332348 1107 52-53 0.10002179564625635 1107 54-55 0.20605665679350693 1107 56-57 0.07608130525082402 1107 58-59 0.1094029384126145 1107 60-61 0.13976834385569958 1107 62-63 0.3719307206614033 1107 64-65 0.37843088810335246 1107 66-67 0.3287717879324745 1107 68-69 0.36830306439757265 1107 70-71 0.254922043734247 1107 72-73 0.1972870624631078 1107 74-75 0.16481302046451418 1107 76-77 0.2478291614708148 1107 78-79 0.37574286268377577 1107 80-81 0.5901944177797631 1107 82-83 0.5170560660887595 1107 84-85 0.5494212548706123 1107 86-87 0.4311324270356458 1107 88-89 0.5052976382267893 1107 90-91 0.5478548871202165 1107 92-93 0.43591444836384596 1107 94-95 0.6152213057238285 1107 96-97 0.705989546766947 1107 98-99 0.9613607050114013 1107 100-101 1.1388014978095349 1108 1 -0.019072059449491974 1108 2 -0.027417508855087647 1108 3 0.03235665779668295 1108 4 -0.07518908383919154 1108 5 -0.08394864330723095 1108 6 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63.0 6 149.0 7 325.0 8 849.0 9 1582.0 10 2613.0 11 4008.0 12 5668.0 13 7532.0 14 9589.0 15 12495.0 16 15000.0 17 18841.0 18 17324.0 19 13038.0 20 12398.0 21 12747.0 22 13748.0 23 15413.0 24 18510.0 25 21979.0 26 27343.0 27 33954.0 28 41345.0 29 51462.0 30 62521.0 31 77232.0 32 95945.0 33 122413.0 34 158204.0 35 213279.0 36 300104.0 37 379214.0 38 217625.0 39 11608.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.017725680436605 18.768373416991995 14.912574358151959 23.301326544419442 2 16.134436520401696 19.94308717031515 39.20066531899491 24.721810990288244 3 19.780660753093805 23.640013506432087 29.46135587344433 27.117969867029778 4 13.680926826922308 16.60807035965341 35.76234981043452 33.948653002989765 5 14.304335911805918 36.56160906307815 33.81661534268837 15.317439682427562 6 33.15445628775692 35.230295011865294 16.886397212243327 14.72885148813445 7 29.350467572973898 31.977663101987368 19.984931060100923 18.686938264937815 8 27.081610232451425 34.34290555010898 19.156940905008067 19.418543312431524 9 26.2258412449358 16.641993788947453 19.89164974633276 37.240515219783994 10-11 25.542388254180487 26.114314641420716 26.662069480301692 21.681227624097104 12-13 26.324019805159594 23.26960972052903 27.380705774851695 23.025664699459682 14-15 23.02208190514169 24.93737832608759 25.619077868298103 26.42146190047262 16-17 22.74147648639692 27.890339738205878 26.46045897759668 22.907724797800523 18-19 22.581743237888364 26.36683751144072 28.83437641991445 22.217042830756466 20-21 23.184431496797473 25.517562858861158 29.12867390039418 22.169331743947186 22-23 22.846954550965222 25.41284968984924 29.076596367163194 22.663599392022345 24-25 22.805448724210812 25.60069774769968 29.197394767994382 22.396458760095122 26-27 22.522627007323408 25.907084234689638 28.93158342459618 22.638705333390778 28-29 22.535922178341135 26.086157117227966 28.92438620055299 22.453534503877908 30-31 22.656932178366873 25.767596648119138 28.874428707906734 22.70104246560725 32-33 22.46235688376892 25.811681846315544 28.898175661780673 22.827785608134864 34-35 22.97393673904499 25.891489879234186 28.683465011131176 22.451108370589644 36-37 23.052134306575194 25.690138135345684 28.62471886694046 22.633008691138663 38-39 22.756702350074825 25.840301927024285 28.683142457911632 22.71985326498926 40-41 22.980031260645653 25.80183055126946 28.609352143157725 22.60878604492716 42-43 22.856534540995945 25.81716986637978 28.388855004089137 22.93744058853514 44-45 22.92949197561587 25.947725466395177 28.40081667843525 22.721965879553707 46-47 23.221996065439743 26.215648368339767 27.804275916172926 22.758079650047566 48-49 23.238290042346666 26.292404902855154 27.575128635573705 22.894176419224475 50-51 23.169087377278927 26.47576513199834 27.566168247006413 22.788979243716316 52-53 23.49141866499541 26.432470628431215 27.340188181436304 22.735922525137074 54-55 23.10530525300128 26.519276401334295 27.38531235683066 22.99010598883376 56-57 23.14531348080951 26.6817509376946 27.236928259287147 22.936007322208745 58-59 23.24728876188797 26.459870469747553 27.295384683207956 22.99745608515652 60-61 23.135093106634706 26.468641028139523 27.623284035667062 22.77298182955871 62-63 22.9818288573036 26.51851606926987 27.70421682129497 22.795438252131557 64-65 22.99482266604142 26.457609388339126 27.52749487978004 23.020073065839416 66-67 22.917446313927943 26.67956685428299 27.302883880777216 23.10010295101185 68-69 23.093499644431375 26.422994656826802 27.51284568371751 22.970660015024308 70-71 23.05484989407146 26.371623073736185 27.309400012123298 23.26412702006906 72-73 23.050313882519617 26.505200816179713 27.286187415743168 23.158297885557495 74-75 23.04849764196257 26.747912309614186 27.021904059802242 23.181685988621002 76-77 23.128852627457796 26.621726182499412 27.080941716223183 23.168479473819605 78-79 23.1239764400227 26.697274731638792 27.067722660772496 23.111026167566003 80-81 23.25946996928469 26.73371665264747 26.881081002548502 23.12573237551933 82-83 23.196480696005388 26.76836283579312 26.86996214824432 23.165194319957173 84-85 23.080915539492874 26.795767080196125 26.960014909304235 23.163302471006762 86-87 23.218248803415463 26.795002048997596 26.818197302938028 23.16855184464891 88-89 23.09024202917937 26.761336052773572 26.7085304150954 23.439891502951653 90-91 23.01852940638613 26.737787869397582 26.89292815323942 23.350754570976868 92-93 22.989588875295947 26.587243140991536 27.12292862102444 23.300239362688078 94-95 22.852872417889856 26.535351554231983 27.16910778414765 23.44266824373051 96-97 23.04866654810421 26.3755457421467 27.041683052498954 23.534104657250136 98-99 22.752414961590063 26.48452130105837 26.908949360201756 23.85411437714982 100-101 22.588714234757063 26.457838194213434 27.01440629396134 23.939041277068164 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 216.0 1 165.5 2 212.5 3 321.5 4 268.5 5 215.0 6 352.5 7 699.5 8 1129.0 9 1408.0 10 1380.0 11 1307.0 12 1401.0 13 1506.0 14 1639.0 15 1857.5 16 2063.0 17 2228.0 18 2475.0 19 2769.5 20 3037.5 21 3451.0 22 4097.0 23 4958.5 24 5972.5 25 7272.5 26 9155.0 27 11554.0 28 14167.0 29 17139.5 30 20887.5 31 25108.5 32 29755.5 33 32662.5 34 34822.0 35 37890.5 36 41853.0 37 45745.5 38 48524.5 39 50764.5 40 52892.0 41 56645.0 42 59639.5 43 62151.0 44 65746.0 45 69314.0 46 71966.0 47 74186.5 48 76953.5 49 77210.5 50 75292.0 51 74095.5 52 73468.0 53 73549.5 54 73092.0 55 69231.0 56 63474.0 57 58179.0 58 54332.5 59 48858.0 60 40503.0 61 33487.0 62 28244.0 63 22209.5 64 16055.5 65 11902.5 66 9123.5 67 7432.5 68 5911.5 69 4476.5 70 3426.0 71 2542.0 72 1971.0 73 1614.0 74 1283.5 75 1074.0 76 815.5 77 518.0 78 344.5 79 216.5 80 141.0 81 94.5 82 62.5 83 43.0 84 27.0 85 16.0 86 9.5 87 4.5 88 3.0 89 2.0 90 1.5 91 1.5 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.037522061118377845 2 0.006211930011587254 3 0.003657023313273141 4 7.514431465629742E-4 5 8.015393563338392E-4 6 1.001924195417299E-4 7 0.0 8 0.0 9 0.0020038483908345977 10-11 5.26010202594082E-4 12-13 6.262026221358118E-4 14-15 0.0022543294396889227 16-17 0.00242966617388695 18-19 0.0 20-21 2.5048104885432474E-5 22-23 0.0016531749224385432 24-25 0.0011021166149590288 26-27 0.00465894750869044 28-29 0.005410390655253414 30-31 0.0013025014540424887 32-33 5.26010202594082E-4 34-35 0.009443135541808043 36-37 0.010920973730048559 38-39 0.00901731775875569 40-41 0.001953752181063733 42-43 8.265874612192716E-4 44-45 0.0012273571393861913 46-47 1.001924195417299E-4 48-49 8.015393563338392E-4 50-51 8.265874612192716E-4 52-53 0.0026801472227412746 54-55 0.0026300510129704095 56-57 0.0020038483908345977 58-59 0.0011522128247298937 60-61 0.004383418354950683 62-63 0.0041078892012109255 64-65 0.007564527675400607 66-67 0.0032061574253353566 68-69 0.0030308206911373293 70-71 1.001924195417299E-4 72-73 0.0015028862931259484 74-75 0.005685919808993171 76-77 0.0018786078664074356 78-79 0.003105965005793627 80-81 0.004308274040294386 82-83 0.004132937306096358 84-85 0.004508658879377845 86-87 0.003055868796022762 88-89 0.00801539356333839 90-91 0.011196502883788316 92-93 0.0012524052442716236 94-95 0.00222928133480349 96-97 0.0012023090345007587 98-99 0.003055868796022762 100-101 0.00435837025006525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1996159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.61307645888975 #Duplication Level Percentage of deduplicated Percentage of total 1 81.65007147193172 51.940122394178054 2 11.022058909305436 14.022941522640675 3 3.0928307390151555 5.902334348261268 4 1.3440283798835178 3.419911203697918 5 0.7222428770560224 2.2972045680026634 6 0.4426914213946855 1.6896577942124826 7 0.33744176039600476 1.5025995953145386 8 0.23091251887112477 1.1751244574610944 9 0.17056341522916565 0.976505721665605 >10 0.911023671843666 10.37736926634054 >50 0.051878598963742695 2.223521707752261 >100 0.02157990654771641 2.5189435329917518 >500 0.0019678893838821995 0.8403818576963673 >1k 5.510090274870039E-4 0.5304775904448054 >5k 1.5743115071057255E-4 0.5829044393400018 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6116 0.30638841895861 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5525 0.27678155898402884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2990 0.1497876672148862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 2.5048104885432474E-5 12-13 0.0 0.0 0.0 0.0 1.5028862931259484E-4 14-15 0.0 0.0 0.0 0.0 1.5028862931259484E-4 16-17 0.0 0.0 0.0 0.0 2.003848390834598E-4 18-19 0.0 0.0 0.0 0.0 2.003848390834598E-4 20-21 0.0 0.0 0.0 5.009620977086495E-5 2.504810488543247E-4 22-23 0.0 0.0 0.0 2.003848390834598E-4 2.504810488543247E-4 24-25 2.5048104885432474E-5 0.0 0.0 4.75913992823217E-4 2.504810488543247E-4 26-27 5.009620977086495E-5 0.0 0.0 6.011545172503794E-4 2.504810488543247E-4 28-29 5.009620977086495E-5 0.0 0.0 9.01731775875569E-4 2.504810488543247E-4 30-31 5.009620977086495E-5 2.5048104885432474E-5 0.0 0.0017032711322094083 2.504810488543247E-4 32-33 5.009620977086495E-5 5.009620977086495E-5 0.0 0.003055868796022762 2.504810488543247E-4 34-35 7.514431465629742E-5 5.009620977086495E-5 0.0 0.004283225935408953 3.005772586251897E-4 36-37 1.001924195417299E-4 5.009620977086495E-5 0.0 0.006312122431128983 3.005772586251897E-4 38-39 1.001924195417299E-4 5.009620977086495E-5 0.0 0.010019241954172989 4.007696781669196E-4 40-41 1.001924195417299E-4 5.009620977086495E-5 0.0 0.017784154468657054 4.007696781669196E-4 42-43 1.2524052442716238E-4 5.009620977086495E-5 0.0 0.02419646931932777 4.007696781669196E-4 44-45 2.003848390834598E-4 5.009620977086495E-5 0.0 0.032287007197322456 4.007696781669196E-4 46-47 2.003848390834598E-4 5.009620977086495E-5 0.0 0.04771663980674887 4.007696781669196E-4 48-49 2.003848390834598E-4 5.009620977086495E-5 0.0 0.057460352607182096 4.007696781669196E-4 50-51 2.003848390834598E-4 5.009620977086495E-5 0.0 0.0691077213789082 4.007696781669196E-4 52-53 2.003848390834598E-4 5.009620977086495E-5 0.0 0.08100557119948862 4.25817783052352E-4 54-55 2.003848390834598E-4 1.001924195417299E-4 0.0 0.10026756385638619 4.508658879377845E-4 56-57 2.003848390834598E-4 1.001924195417299E-4 0.0 0.1445526132938308 4.508658879377845E-4 58-59 2.003848390834598E-4 1.001924195417299E-4 0.0 0.17916909424549848 4.508658879377845E-4 60-61 2.003848390834598E-4 1.001924195417299E-4 0.0 0.20496864227749395 4.75913992823217E-4 62-63 2.003848390834598E-4 1.001924195417299E-4 0.0 0.2355774264474924 6.011545172503794E-4 64-65 2.003848390834598E-4 1.001924195417299E-4 0.0 0.2679395779594712 6.262026221358118E-4 66-67 2.003848390834598E-4 1.001924195417299E-4 0.0 0.3259760369790182 6.512507270212443E-4 68-69 2.504810488543247E-4 1.001924195417299E-4 0.0 0.3983400119930326 6.512507270212443E-4 70-71 2.504810488543247E-4 1.001924195417299E-4 0.0 0.4351356780697329 6.512507270212443E-4 72-73 2.504810488543247E-4 1.001924195417299E-4 0.0 0.4747868281033725 6.762988319066768E-4 74-75 2.504810488543247E-4 1.001924195417299E-4 0.0 0.5508829707453163 7.013469367921092E-4 76-77 2.504810488543247E-4 1.001924195417299E-4 0.0 0.639478117725091 7.263950416775417E-4 78-79 2.504810488543247E-4 1.001924195417299E-4 0.0 0.7250424440137284 7.514431465629742E-4 80-81 3.506734683960546E-4 1.001924195417299E-4 0.0 0.8029670983123087 7.764912514484066E-4 82-83 3.506734683960546E-4 1.001924195417299E-4 0.0 0.9224966548255926 8.015393563338392E-4 84-85 3.506734683960546E-4 1.001924195417299E-4 0.0 1.1245096207266054 8.015393563338392E-4 86-87 3.506734683960546E-4 1.001924195417299E-4 0.0 1.311969637689182 8.015393563338392E-4 88-89 3.506734683960546E-4 1.001924195417299E-4 0.0 1.5553620728609294 8.015393563338392E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4485 0.0 45.883533 1 GTATCAA 7355 0.0 37.025707 1 ATCAACG 9280 0.0 29.021257 3 TATCAAC 9340 0.0 28.989567 2 TCAACGC 9310 0.0 28.977306 4 CAACGCA 9660 0.0 27.878237 5 AACGCAG 9875 0.0 27.368147 6 TACCTGG 1695 0.0 26.623653 2 GTATACG 115 1.2774576E-4 24.796183 1 GTACCTG 1880 0.0 24.7742 1 ACGCAGA 11290 0.0 23.89597 7 CGCAGAG 11445 0.0 23.447845 8 GTACATG 6660 0.0 23.192125 1 TACATGG 6655 0.0 22.270004 2 ACATGGG 6570 0.0 21.544283 3 GAGTACT 7745 0.0 21.43445 12-13 GTATAGC 725 0.0 20.977001 1 GTGTAGC 1225 0.0 20.950243 1 GCAGAGT 12705 0.0 20.936037 9 GTACTTT 8130 0.0 20.097824 14-15 >>END_MODULE