FastQCFastQC Report
Thu 26 May 2016
SRR936436_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936436_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077143
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50490.24307426113657077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42140.2028748141076469No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24240.1166987540097143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA73450.039.6597821
GGTATCA42700.039.3964461
TCAACGC91250.031.455474
ATCAACG91250.031.4547143
TATCAAC91650.031.367772
CAACGCA93050.030.7959125
AACGCAG95100.030.2812466
ACGCAGA108050.026.1682017
CGCAGAG110950.025.5754268
GTACATG73750.024.6784781
GTACCTG20450.023.9345661
TACATGG74450.023.679362
GCAGAGT118600.023.2054849
TACCTGG19650.022.7313922
ACATGGG76100.022.5426123
GAGTACT66350.021.05963312-13
AGAGTAC101900.020.4541610-11
AGTACTT69400.020.20258712-13
GTACTTT69700.019.87242314-15
CATGGGA34100.019.6497424
Produced by FastQC (version 0.11.5)