FastQCFastQC Report
Thu 26 May 2016
SRR936436_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936436_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077143
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.299160914775728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55750.2683975056122761No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.15261346955890856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.046.052541
GTATCAA77300.035.709251
ATCAACG99000.027.3955763
TATCAAC100050.027.2999382
TCAACGC100350.027.0704484
CAACGCA103650.026.3002195
AACGCAG106200.025.8022526
TACCTGG18700.023.6239342
GTACATG73450.023.4153611
GTACCTG19800.023.2750511
TACATGG74950.022.372472
ACGCAGA122250.022.2981767
CGCAGAG124950.021.7403288
ACATGGG74250.021.4942533
GAGTACT79750.021.11119312-13
GTAAGGT8450.020.7951374
GCAGAGT133950.020.3150659
CATGGGG46150.020.1697794
GTACTTT83550.019.75311714-15
AGAGTAC116450.019.41215710-11
Produced by FastQC (version 0.11.5)