##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936436_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2077143 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.875999870976624 31.0 31.0 34.0 30.0 34.0 2 32.040871524011585 31.0 31.0 34.0 30.0 34.0 3 32.123006937894985 33.0 31.0 34.0 30.0 34.0 4 35.824141621448305 37.0 35.0 37.0 35.0 37.0 5 35.644817424703064 37.0 35.0 37.0 33.0 37.0 6 35.72389864347327 37.0 35.0 37.0 33.0 37.0 7 35.669854218029286 37.0 35.0 37.0 33.0 37.0 8 35.634660685374094 37.0 35.0 37.0 33.0 37.0 9 37.26967666645965 39.0 37.0 39.0 34.0 39.0 10-11 37.16156903978205 39.0 37.0 39.0 33.0 39.0 12-13 37.05057210793865 39.0 37.0 39.0 33.0 39.0 14-15 38.196576499547696 40.0 37.5 41.0 33.0 41.0 16-17 38.085100544353466 40.0 37.0 41.0 33.0 41.0 18-19 38.21415136078738 40.0 37.5 41.0 33.0 41.0 20-21 38.224989564993834 40.0 38.0 41.0 33.0 41.0 22-23 38.13685047201854 40.0 37.0 41.0 33.0 41.0 24-25 37.99970151308793 40.0 37.0 41.0 33.0 41.0 26-27 37.80009561209796 40.0 37.0 41.0 33.0 41.0 28-29 37.719161367320396 40.0 37.0 41.0 32.5 41.0 30-31 37.695025811896436 40.0 37.0 41.0 33.0 41.0 32-33 37.545358697017974 40.0 36.5 41.0 32.0 41.0 34-35 37.381489382290965 39.5 36.0 41.0 32.0 41.0 36-37 37.181486541851 39.0 36.0 41.0 31.0 41.0 38-39 37.01404982709423 39.0 36.0 41.0 31.0 41.0 40-41 36.81885527380638 39.0 35.5 41.0 31.0 41.0 42-43 36.5494920186044 39.0 35.0 40.5 30.0 41.0 44-45 36.2981405228239 39.0 35.0 40.0 30.0 41.0 46-47 35.95093693597407 39.0 35.0 40.0 29.5 41.0 48-49 35.58502712620171 38.0 35.0 40.0 28.0 41.0 50-51 35.29733821889008 38.0 34.0 40.0 27.0 41.0 52-53 34.92169966150621 38.0 34.0 40.0 26.0 41.0 54-55 34.73012016986794 38.0 34.0 40.0 26.0 41.0 56-57 34.42116118148823 38.0 33.5 40.0 25.5 41.0 58-59 34.033004468156506 37.0 33.0 40.0 24.5 41.0 60-61 33.61412454510835 36.5 32.5 39.5 23.0 41.0 62-63 33.2666138055974 36.0 32.0 39.0 23.0 40.0 64-65 32.837690038673315 35.5 31.5 39.0 22.0 40.0 66-67 32.41432173904252 35.0 31.0 38.5 21.5 40.0 68-69 32.050614955253444 35.0 31.0 38.0 20.0 40.0 70-71 31.67877368096467 35.0 31.0 37.5 19.5 39.5 72-73 31.21882629169008 34.5 30.0 37.0 18.0 39.0 74-75 30.720859613420934 34.0 30.0 36.0 17.0 39.0 76-77 29.237009681085993 32.5 28.0 35.0 13.0 37.0 78-79 30.00601956629852 34.0 29.0 35.0 13.0 37.0 80-81 29.791483542538955 34.0 29.0 35.0 8.0 37.0 82-83 29.501268087945796 34.0 29.0 35.0 7.0 36.5 84-85 29.133359378723565 34.0 29.0 35.0 6.0 36.0 86-87 28.747018861965692 33.5 29.0 35.0 2.0 36.0 88-89 28.287695888053925 33.0 27.5 35.0 2.0 35.0 90-91 27.823102694422097 33.0 27.0 35.0 2.0 35.0 92-93 27.204760336673978 33.0 26.0 34.5 2.0 35.0 94-95 26.650880319746882 32.0 25.0 34.0 2.0 35.0 96-97 25.909097495935523 32.0 24.5 34.0 2.0 35.0 98-99 24.987731465768128 32.0 20.5 34.0 2.0 35.0 100-101 21.710088328054447 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3497136600328936 1101 2 0.3313909026925046 1101 3 0.36992305370015544 1101 4 0.3724455102827946 1101 5 0.3432259737618608 1101 6 0.23985850938152709 1101 7 0.36283601353759565 1101 8 0.2315836097667585 1101 9 0.30765064410549314 1101 10-11 0.3013186218252457 1101 12-13 0.29883586354569047 1101 14-15 0.4766151282148101 1101 16-17 0.4786221324837925 1101 18-19 0.39599089773580687 1101 20-21 0.43526753892569303 1101 22-23 0.36474790261272716 1101 24-25 0.3318069418242473 1101 26-27 0.28508605700230305 1101 28-29 0.35751503292615894 1101 30-31 0.42961194714909823 1101 32-33 0.33187535231137133 1101 34-35 0.3502427119568523 1101 36-37 0.4174041089134377 1101 38-39 0.31965336141023926 1101 40-41 0.45109764506594985 1101 42-43 0.5651424571193004 1101 44-45 0.5804574091765815 1101 46-47 0.5351121377906125 1101 48-49 0.6367273831560354 1101 50-51 0.4975134744040304 1101 52-53 0.5913767474545111 1101 54-55 0.44176653667962285 1101 56-57 0.31018841229376903 1101 58-59 0.2866116670875485 1101 60-61 0.43489332789190627 1101 62-63 0.5706199226104047 1101 64-65 0.5827853614132792 1101 66-67 0.2932463332478221 1101 68-69 0.12469959293732558 1101 70-71 0.057273534786663305 1101 72-73 0.20005295912612908 1101 74-75 0.08917628410533851 1101 76-77 0.17119538721818017 1101 78-79 0.4125721627309318 1101 80-81 0.3756735095655266 1101 82-83 0.2881958837305625 1101 84-85 0.33036326579741626 1101 86-87 0.2668801762441326 1101 88-89 0.2571218209641266 1101 90-91 0.30607579639786664 1101 92-93 0.2229737754393888 1101 94-95 0.4617089775903125 1101 96-97 0.7562690341249514 1101 98-99 0.6532203693291336 1101 100-101 1.2293688259893152 1102 1 0.2791398055075085 1102 2 0.20193540292471113 1102 3 0.24955686984588965 1102 4 0.19030869298916997 1102 5 0.20233062400764368 1102 6 0.22581927101590793 1102 7 0.24033441438580638 1102 8 0.2518577876274861 1102 9 0.2718160621193846 1102 10-11 0.30628168799069755 1102 12-13 0.2500634863736906 1102 14-15 0.3991686450343721 1102 16-17 0.46325318844972685 1102 18-19 0.27910254043710125 1102 20-21 0.173174884508974 1102 22-23 0.27065424927378245 1102 24-25 0.30080771292210073 1102 26-27 0.2590163777965415 1102 28-29 0.2224782743411282 1102 30-31 0.3503730995898877 1102 32-33 0.3820729461355725 1102 34-35 0.3364332695131722 1102 36-37 0.26636312681517893 1102 38-39 0.3089379490919484 1102 40-41 0.3849135717415493 1102 42-43 0.4056811741176318 1102 44-45 0.2737926702545366 1102 46-47 0.3406412598781827 1102 48-49 0.48435099978020446 1102 50-51 0.29115826993166394 1102 52-53 0.3903684055777603 1102 54-55 0.3602919434775558 1102 56-57 0.2928603977831159 1102 58-59 0.37457706407150226 1102 60-61 0.42233932913617167 1102 62-63 0.42531244734249185 1102 64-65 0.42689807313659145 1102 66-67 0.2015485597928688 1102 68-69 0.0548214789725705 1102 70-71 0.24181148944961706 1102 72-73 0.3814817683933249 1102 74-75 0.31466742086129784 1102 76-77 0.1593570636550048 1102 78-79 0.21159558482813878 1102 80-81 0.16851697670186283 1102 82-83 0.11920343698450253 1102 84-85 0.17492614970337428 1102 86-87 0.24167863930591338 1102 88-89 0.3498675864978047 1102 90-91 0.2715768347512224 1102 92-93 0.2399055069921907 1102 94-95 0.10719893141206782 1102 96-97 0.4481941938210703 1102 98-99 0.24827088500082084 1102 100-101 0.9180674231169768 1103 1 0.19563638048562026 1103 2 0.215549967247199 1103 3 0.17846741007455336 1103 4 0.22363411508117537 1103 5 0.2612369085340802 1103 6 0.12235742093120194 1103 7 0.1471652256699656 1103 8 0.10936746525803898 1103 9 0.16959041169258882 1103 10-11 0.2081308724508375 1103 12-13 0.19202757479409627 1103 14-15 0.19241946957668432 1103 16-17 0.2206421514496668 1103 18-19 0.18131100437411618 1103 20-21 0.17510959221588251 1103 22-23 0.12450156777217813 1103 24-25 0.18396556673872766 1103 26-27 0.10375853952959346 1103 28-29 0.108291645430171 1103 30-31 0.16114514126303447 1103 32-33 0.1374203996706811 1103 34-35 0.11093977173513281 1103 36-37 -0.016587875320851708 1103 38-39 0.08226553995528008 1103 40-41 0.1131123048517324 1103 42-43 0.18879872152370325 1103 44-45 0.2223764216560582 1103 46-47 0.07326285429338952 1103 48-49 0.060265771158974246 1103 50-51 0.12288718988354219 1103 52-53 -0.004832676352691578 1103 54-55 0.07828312926350378 1103 56-57 -0.02033412054505135 1103 58-59 0.11908659438928737 1103 60-61 0.05942695568193557 1103 62-63 0.17167689095034433 1103 64-65 0.17685899433244145 1103 66-67 -0.10996700216443145 1103 68-69 0.16703370756925295 1103 70-71 0.05339579736603994 1103 72-73 0.07695476514425792 1103 74-75 -0.07224281655778242 1103 76-77 -0.12622654043374482 1103 78-79 -0.14707704527767973 1103 80-81 0.022173811605167515 1103 82-83 -0.010891948033783905 1103 84-85 0.36346685304000914 1103 86-87 0.1784260142742049 1103 88-89 -0.009944057758222158 1103 90-91 -0.03665569845553307 1103 92-93 0.01419451827798568 1103 94-95 0.02101482318628456 1103 96-97 0.18718481490807548 1103 98-99 0.11283438908056809 1103 100-101 0.5142458843532545 1104 1 0.19520126698181528 1104 2 0.16731280075623545 1104 3 0.19973650672277898 1104 4 0.1407040545083902 1104 5 0.14137678426940425 1104 6 0.1532820605199774 1104 7 0.13441281305136243 1104 8 0.06353979827738954 1104 9 0.05555090525790263 1104 10-11 0.19581864186251607 1104 12-13 0.22053647668613507 1104 14-15 0.22515825175792514 1104 16-17 0.31449059782933375 1104 18-19 0.19657623646584454 1104 20-21 0.03868259927127582 1104 22-23 0.09446976861682543 1104 24-25 0.15601517747589355 1104 26-27 0.16963339839062286 1104 28-29 0.1926826112885749 1104 30-31 0.12045754496102745 1104 32-33 -0.034639699017439796 1104 34-35 -0.03982218374916613 1104 36-37 0.043978286884467366 1104 38-39 0.08971880814114996 1104 40-41 0.05167513586550143 1104 42-43 -0.21526576884765802 1104 44-45 -0.21089408794139075 1104 46-47 -0.2417094169679075 1104 48-49 -0.16501385554794723 1104 50-51 -0.1838409281179878 1104 52-53 -0.1361472805709596 1104 54-55 -0.1328453664602165 1104 56-57 -0.26612665873834374 1104 58-59 -0.1358944025352926 1104 60-61 0.01046284552803911 1104 62-63 -0.23117934068599766 1104 64-65 -0.22510439687045647 1104 66-67 -0.20227032774161557 1104 68-69 -0.40346988562613717 1104 70-71 -0.264929304160443 1104 72-73 -0.07121639353648845 1104 74-75 -0.2444058453220599 1104 76-77 -0.34284990368957935 1104 78-79 -0.47138261816153104 1104 80-81 -0.08239203401219797 1104 82-83 -0.24350787693093778 1104 84-85 0.011411682519181454 1104 86-87 -0.07358287670101404 1104 88-89 -0.16025783853587328 1104 90-91 -0.03188718975637883 1104 92-93 0.08529268861790129 1104 94-95 0.012340235062541893 1104 96-97 0.3503289856347571 1104 98-99 0.35626031529530167 1104 100-101 1.0536716125055676 1105 1 0.0852395047562311 1105 2 0.11603296935560081 1105 3 0.09795126540771548 1105 4 0.12781992622917926 1105 5 0.218438191516924 1105 6 0.05717369022244867 1105 7 0.0642521291553706 1105 8 0.10437083815610038 1105 9 0.05168869215537342 1105 10-11 0.08082047611881649 1105 12-13 0.17053034981344695 1105 14-15 0.1762958712129219 1105 16-17 0.09858207774689731 1105 18-19 0.06942098531734331 1105 20-21 0.23819307736523143 1105 22-23 0.22445517828769823 1105 24-25 0.13868649777509034 1105 26-27 0.08144391590967359 1105 28-29 0.038316244090253804 1105 30-31 0.18611507256620996 1105 32-33 0.06572913110760226 1105 34-35 0.28018808732499423 1105 36-37 0.12238494226652108 1105 38-39 0.14882134306812844 1105 40-41 0.2519015257142243 1105 42-43 0.21759360077705736 1105 44-45 0.17157517875185846 1105 46-47 0.03548095904702109 1105 48-49 0.1520803475932624 1105 50-51 0.14227290689331795 1105 52-53 0.30202191853642546 1105 54-55 0.266260738036074 1105 56-57 0.0667498547133718 1105 58-59 -0.0031788241432835207 1105 60-61 0.17936464736138902 1105 62-63 0.07938327366960607 1105 64-65 0.05729781671916356 1105 66-67 0.0022781712126302978 1105 68-69 0.02966481271861454 1105 70-71 0.25986046038498145 1105 72-73 0.23086668609611039 1105 74-75 0.17740232039119874 1105 76-77 -0.040002392037106205 1105 78-79 -0.04653109017275625 1105 80-81 -1.2940381957093905E-6 1105 82-83 0.314585626757367 1105 84-85 0.42127739364741146 1105 86-87 0.4415357524930421 1105 88-89 0.26534898824639797 1105 90-91 0.5013245511583406 1105 92-93 0.3274906299886773 1105 94-95 0.4846667494275856 1105 96-97 0.39869474699573004 1105 98-99 0.10110565552320594 1105 100-101 0.6612981229128785 1106 1 0.003572947115156211 1106 2 -0.031119056678207357 1106 3 -0.041935896866725386 1106 4 -0.0051247496753390465 1106 5 0.12076085011720039 1106 6 -0.03892221620502312 1106 7 -0.015100454337556357 1106 8 0.02238115122495543 1106 9 -0.07740832031790745 1106 10-11 -0.04222213860055035 1106 12-13 0.0163676964163173 1106 14-15 0.13810044773121177 1106 16-17 -0.02368213185893353 1106 18-19 0.05187670025076585 1106 20-21 -0.06944367643919946 1106 22-23 -0.07542844629433887 1106 24-25 -0.05299035714492817 1106 26-27 -0.1832016982916258 1106 28-29 -0.4094919844093283 1106 30-31 -0.11048039663106124 1106 32-33 -0.02421359662242395 1106 34-35 0.10066335618586209 1106 36-37 -0.27075005472211444 1106 38-39 -0.005836812835454452 1106 40-41 0.1028054606357216 1106 42-43 0.20745939027646187 1106 44-45 0.1433993949142618 1106 46-47 0.03265136905091026 1106 48-49 -0.030647574160944657 1106 50-51 -0.04172149746229081 1106 52-53 -0.2064653476506777 1106 54-55 -0.0947812608766796 1106 56-57 -0.40507379752357053 1106 58-59 -0.10172461075539019 1106 60-61 -0.19429208218672755 1106 62-63 -0.07959398735194156 1106 64-65 -0.21001704467170867 1106 66-67 -0.02573313436504776 1106 68-69 -0.25165203594559316 1106 70-71 -9.263058634019217E-4 1106 72-73 -0.06451061011438597 1106 74-75 0.11601029294381959 1106 76-77 -0.1031914619585983 1106 78-79 -0.034362330590724355 1106 80-81 0.1378412624840557 1106 82-83 0.08166204166514746 1106 84-85 0.35565573725546784 1106 86-87 0.36382691773598097 1106 88-89 0.3813046576898351 1106 90-91 0.41699720520961847 1106 92-93 0.5057351563401618 1106 94-95 0.3705801169423246 1106 96-97 0.46931729438990644 1106 98-99 0.30010376674885464 1106 100-101 0.938583656420203 1107 1 0.08630542598071145 1107 2 0.07202811176102131 1107 3 -0.016294409729191273 1107 4 0.07835311597323624 1107 5 0.03545278721382772 1107 6 0.03916741201059182 1107 7 0.012642478123133571 1107 8 0.07868057854974353 1107 9 0.023652855938799178 1107 10-11 0.14657333453826027 1107 12-13 0.09617744098498804 1107 14-15 0.19773793523234673 1107 16-17 0.2147973700045327 1107 18-19 0.06097023720194983 1107 20-21 0.04183929783587104 1107 22-23 0.021798623971996278 1107 24-25 0.13176039278689444 1107 26-27 0.19853641642522035 1107 28-29 0.11306060171920507 1107 30-31 0.0040568272576848585 1107 32-33 -0.003710733246364839 1107 34-35 0.24191033373335813 1107 36-37 0.2537189935462223 1107 38-39 0.16543770766268295 1107 40-41 0.2136653824610093 1107 42-43 0.25849720502058204 1107 44-45 0.3467796330744264 1107 46-47 0.20930567320507976 1107 48-49 0.26124525376378926 1107 50-51 0.24211288123055397 1107 52-53 0.45988989278200876 1107 54-55 0.30535181793432997 1107 56-57 0.2021404557261377 1107 58-59 0.1334505551386158 1107 60-61 0.28541803507533103 1107 62-63 0.34383924599048754 1107 64-65 0.3134441861254871 1107 66-67 0.4449000807972183 1107 68-69 0.5419899965969037 1107 70-71 0.46858937452929084 1107 72-73 0.5520341585430124 1107 74-75 0.598037290489497 1107 76-77 0.4072667691113061 1107 78-79 0.482039814668795 1107 80-81 0.7736492799370147 1107 82-83 0.7714791492008253 1107 84-85 0.9673954881872007 1107 86-87 1.006290421053862 1107 88-89 0.8239453267199224 1107 90-91 0.7860543818299277 1107 92-93 0.8962444736749511 1107 94-95 1.066317231750066 1107 96-97 1.3926852609384035 1107 98-99 1.259191213344618 1107 100-101 1.7855768388581623 1108 1 0.10724992946946088 1108 2 0.11945882198698143 1108 3 0.06282337595538934 1108 4 0.1119726344797769 1108 5 0.1311777537126062 1108 6 0.11580324214783388 1108 7 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-1.815679942337951 2316 60-61 -1.322348367231804 2316 62-63 -1.0368875023873017 2316 64-65 -1.0328889068809985 2316 66-67 -1.0159737476094186 2316 68-69 -1.5193308397405154 2316 70-71 -1.5638143745694855 2316 72-73 -1.0606031882486242 2316 74-75 -1.0585077707938488 2316 76-77 -1.0481564464618742 2316 78-79 -1.364823920010366 2316 80-81 -1.0676254136462582 2316 82-83 -1.136419913524481 2316 84-85 -0.8872606040983086 2316 86-87 -1.1645617779367647 2316 88-89 -1.2858311693146867 2316 90-91 -1.4218446808953757 2316 92-93 -1.476787970449056 2316 94-95 -1.4450079304705525 2316 96-97 -1.802305366314659 2316 98-99 -2.398394943829139 2316 100-101 -3.28650967880586 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 13.0 4 26.0 5 89.0 6 159.0 7 334.0 8 899.0 9 1651.0 10 2668.0 11 4188.0 12 5833.0 13 7972.0 14 10126.0 15 12912.0 16 15638.0 17 19579.0 18 18016.0 19 13434.0 20 12782.0 21 13608.0 22 14413.0 23 16140.0 24 19081.0 25 23129.0 26 28360.0 27 35097.0 28 43310.0 29 53595.0 30 65992.0 31 80455.0 32 99813.0 33 126636.0 34 164519.0 35 221334.0 36 311071.0 37 393645.0 38 228059.0 39 12567.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.98175455052806 18.7771489601235 14.897071704313063 23.34402478503537 2 16.21304356409233 19.90956588059634 39.087020811595906 24.790369743715427 3 19.852476341567343 23.598970608414724 29.425818084647958 27.122734965369975 4 13.61225490974863 16.59216529627474 35.803071815469615 33.992507978507014 5 14.313535082368473 36.55140970280718 33.78375971140102 15.351295503423334 6 33.18086429292543 35.180726603801475 16.895755371681197 14.742653731591904 7 29.434083257628384 31.855774975531293 20.034537824309638 18.675603942530678 8 27.090720282618964 34.348236977425245 19.112742839563765 19.44829990039203 9 26.28437233257412 16.61734411159944 19.8364773152354 37.26180624059104 10-11 25.560854500628988 26.067680463020597 26.64510820872708 21.726356827623327 12-13 26.336255569311252 23.248614246797068 27.406174753065482 23.0089554308262 14-15 23.09086606845616 24.8509789544708 25.55904406035319 26.499110916719843 16-17 22.724704732583344 27.889877560157768 26.411572629225006 22.973845078033886 18-19 22.637445380999424 26.350743049497478 28.813580945888432 22.198230623614666 20-21 23.208946134185275 25.588681183722063 29.00445467644741 22.197918005645253 22-23 22.88707619300485 25.411605720270593 29.0361335865983 22.665184500126255 24-25 22.848210258032307 25.585503742400018 29.10533843842239 22.460947561145282 26-27 22.58086646727127 25.87457347640053 28.858330976295214 22.68622908003298 28-29 22.538079252990673 26.085386593936448 28.874105867244076 22.502428285828803 30-31 22.711057061810703 25.765677025260025 28.827013031530257 22.69625288139902 32-33 22.514343462647776 25.80658129799761 28.823372652223178 22.855702587131436 34-35 23.013260058182013 25.931002442103846 28.598781234917393 22.456956264796755 36-37 23.03899160691465 25.68945608271812 28.57620852653895 22.695343783828285 38-39 22.72285117786859 25.9160457951284 28.67727221410602 22.68383081289699 40-41 23.018643922551128 25.73854024142835 28.582742215490736 22.660073620529786 42-43 22.897625633805056 25.83392171112861 28.29817550324657 22.970277151819765 44-45 22.953102947913052 25.91649421179921 28.28297538868242 22.847427451605316 46-47 23.24704238689794 26.205148956052305 27.732417586782205 22.815391070267548 48-49 23.284393197439062 26.313356966910344 27.509575113154956 22.892674722495638 50-51 23.203119094948295 26.468063554664802 27.47117311727995 22.85764423310695 52-53 23.548945169922714 26.438116338582628 27.210574370328754 22.802364121165905 54-55 23.1647982899358 26.44314349470299 27.389552335811935 23.00250587954928 56-57 23.132008534106816 26.675463672506783 27.231018425562258 22.961509367824146 58-59 23.27317406971688 26.396357698332707 27.253913731057366 23.076554500893046 60-61 23.145875833071102 26.518391787852863 27.523963772828274 22.81176860624776 62-63 23.05776508768044 26.486234026366727 27.587680440665235 22.868320445287598 64-65 22.992068695120622 26.46679062515574 27.46485468663967 23.07628599308397 66-67 22.924215273839316 26.643786944644777 27.28983377738321 23.1421640041327 68-69 23.17346003416262 26.356659496496114 27.449805596584508 23.020074872756755 70-71 23.109943694227713 26.413668213584973 27.255281960945833 23.221106131241484 72-73 23.109325217053048 26.49525164865955 27.1605648530529 23.234858281234505 74-75 23.089059012961027 26.777546607392043 26.931149338877567 23.202245040769355 76-77 23.195466568324523 26.604615469052558 27.007893690343565 23.192024272279358 78-79 23.15418864899414 26.66953144089718 26.995730523447214 23.18054938666147 80-81 23.306316771494778 26.701688284329954 26.83602877005026 23.155966174125012 82-83 23.228906925810247 26.74507936408577 26.88238263916492 23.143631070939065 84-85 23.151519325077224 26.813290323170953 26.862795190226546 23.17239516152528 86-87 23.2286712672235 26.78484632912398 26.77264077017799 23.213841633474527 88-89 23.12653577445415 26.757513555271505 26.60825867378441 23.507691996489932 90-91 23.04582168049835 26.75124860834265 26.831129680189132 23.37180003096987 92-93 23.041502172336443 26.55699361349459 26.997480455713905 23.404023758455065 94-95 22.908569762337773 26.50142219784406 27.063559349581357 23.52644869023681 96-97 23.053562332847253 26.376688385736458 27.019132931062927 23.550616350353362 98-99 22.769783956080744 26.497333318883044 26.825379049242986 23.907503675793233 100-101 22.641034089420177 26.440399392664183 26.9338838277571 23.98468269015854 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 232.0 1 180.0 2 227.0 3 319.5 4 261.5 5 215.0 6 329.0 7 681.0 8 1126.0 9 1427.0 10 1422.0 11 1324.5 12 1374.5 13 1492.5 14 1670.5 15 1839.5 16 2039.0 17 2263.5 18 2506.5 19 2851.5 20 3157.0 21 3546.0 22 4206.5 23 5105.5 24 6243.5 25 7603.0 26 9534.5 27 11931.5 28 14413.5 29 17475.0 30 21434.5 31 26022.0 32 31086.5 33 34107.5 34 36268.0 35 39582.5 36 43269.0 37 47100.0 38 50236.0 39 52425.5 40 54766.5 41 58255.5 42 61444.0 43 64637.0 44 68336.0 45 72025.5 46 74628.5 47 76767.5 48 79378.0 49 79909.5 50 78543.0 51 77509.5 52 77042.5 53 77048.5 54 76732.0 55 72610.0 56 66163.5 57 60569.0 58 56895.0 59 51578.5 60 42682.0 61 35253.5 62 29684.0 63 23275.0 64 16865.5 65 12586.0 66 9842.0 67 7878.0 68 6139.0 69 4771.5 70 3641.0 71 2692.5 72 2078.0 73 1669.5 74 1348.5 75 1099.5 76 797.0 77 504.0 78 356.0 79 260.5 80 173.5 81 110.0 82 76.5 83 45.5 84 24.0 85 14.5 86 8.5 87 3.5 88 4.0 89 4.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019546078435620465 2 0.013094909690859032 3 0.009050893462799624 4 0.0 5 0.0035625857247190006 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.001131361682849953 14-15 1.6850067616914195E-4 16-17 2.4071525167020277E-5 18-19 0.001299862359019095 20-21 0.0 22-23 1.6850067616914195E-4 24-25 0.0 26-27 0.001468363035188237 28-29 0.0029126545452094533 30-31 0.001299862359019095 32-33 0.007293672125607144 34-35 0.0019497935385286425 36-37 7.943603305116692E-4 38-39 0.0010832186325159124 40-41 2.407152516702028E-4 42-43 0.005030948759907238 44-45 0.0014202199848541964 46-47 1.6850067616914195E-4 48-49 6.499311795095475E-4 50-51 9.628610066808111E-5 52-53 3.6107287750530414E-4 54-55 0.0013480054093531356 56-57 0.014442915100212167 58-59 0.026189819381718063 60-61 0.01995529436345981 62-63 0.013624483244533477 64-65 0.017933286249430105 66-67 0.002359009466367987 68-69 9.147179563467707E-4 70-71 1.203576258351014E-4 72-73 4.8143050334040555E-5 74-75 0.0015165060855222776 76-77 0.001973865063695663 78-79 0.009171251088634726 80-81 0.015911278135400404 82-83 0.017018568293083337 84-85 0.027898897668576502 86-87 0.010615542598655943 88-89 0.03991058872691962 90-91 0.04484525138615878 92-93 0.0277785400427414 94-95 0.029054330876593475 96-97 0.04864855236254798 98-99 0.05105570487925001 100-101 0.057338372947842296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2077143.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.69249945650397 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97878505541152 50.76762438074738 2 11.390949420028889 14.282541806484506 3 3.2699176715485923 6.149979355391186 4 1.3925891434484425 3.4921957647509925 5 0.7298666384647166 2.287858191763481 6 0.4654072274847165 1.7506525413683165 7 0.3207003451698554 1.4073854350683255 8 0.2367179025173288 1.1872349579929915 9 0.166594814090082 0.9399820762618961 >10 0.9689595684588284 10.875768343525463 >50 0.05375348603231975 2.2647302958333553 >100 0.022915685743669468 2.5710525420937786 >500 0.002074651979137662 0.8661855141103585 >1k 6.147116975222392E-4 0.5889059512818371 >5k 1.536779243805598E-4 0.5679028433261004 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6214 0.299160914775728 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5575 0.2683975056122761 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3170 0.15261346955890856 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.8143050334040555E-5 7 0.0 0.0 0.0 0.0 4.8143050334040555E-5 8 0.0 0.0 0.0 0.0 9.628610066808111E-5 9 0.0 0.0 0.0 0.0 1.4442915100212168E-4 10-11 0.0 0.0 0.0 0.0 1.4442915100212168E-4 12-13 2.4071525167020277E-5 0.0 0.0 0.0 1.9257220133616222E-4 14-15 4.8143050334040555E-5 0.0 0.0 0.0 2.1664372650318252E-4 16-17 7.221457550106084E-5 0.0 0.0 9.628610066808111E-5 2.8885830200424335E-4 18-19 1.203576258351014E-4 0.0 0.0 1.4442915100212168E-4 2.8885830200424335E-4 20-21 1.4442915100212168E-4 0.0 0.0 1.4442915100212168E-4 3.6107287750530414E-4 22-23 1.4442915100212168E-4 0.0 0.0 1.6850067616914195E-4 4.0921592783934473E-4 24-25 1.9257220133616222E-4 0.0 0.0 2.407152516702028E-4 4.33287453006365E-4 26-27 1.9257220133616222E-4 0.0 0.0 3.370013523382839E-4 4.33287453006365E-4 28-29 1.9257220133616222E-4 0.0 0.0 8.665749060127301E-4 4.33287453006365E-4 30-31 1.9257220133616222E-4 0.0 0.0 0.0016609352365243991 4.33287453006365E-4 32-33 1.9257220133616222E-4 9.628610066808111E-5 0.0 0.0028404399697083927 4.33287453006365E-4 34-35 1.9257220133616222E-4 9.628610066808111E-5 0.0 0.004549518256566833 4.33287453006365E-4 36-37 1.9257220133616222E-4 1.203576258351014E-4 0.0 0.006595597895763556 4.33287453006365E-4 38-39 1.9257220133616222E-4 1.4442915100212168E-4 0.0 0.010302612771484677 4.33287453006365E-4 40-41 1.9257220133616222E-4 1.4442915100212168E-4 0.0 0.016994496767916315 4.33287453006365E-4 42-43 1.9257220133616222E-4 1.4442915100212168E-4 0.0 0.022867948908669264 4.33287453006365E-4 44-45 2.407152516702028E-4 1.4442915100212168E-4 0.0 0.030859695264119996 5.055020285074258E-4 46-47 2.407152516702028E-4 1.4442915100212168E-4 0.0 0.04359353207747372 5.777166040084867E-4 48-49 2.8885830200424335E-4 1.4442915100212168E-4 0.0 0.05288514079194355 6.258596543425272E-4 50-51 2.8885830200424335E-4 1.4442915100212168E-4 0.0 0.06439132982177924 6.258596543425272E-4 52-53 2.8885830200424335E-4 1.4442915100212168E-4 0.0 0.07659559308145852 7.221457550106083E-4 54-55 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.09308458782086741 7.702888053446489E-4 56-57 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.13540232906448907 8.425033808457097E-4 58-59 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.16996903920433018 8.6657490601273E-4 60-61 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.19401649284618344 8.906464311797503E-4 62-63 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.22511690336197365 9.147179563467706E-4 64-65 3.370013523382839E-4 1.4442915100212168E-4 0.0 0.2565302437049351 9.147179563467706E-4 66-67 3.8514440267232444E-4 1.6850067616914195E-4 0.0 0.3119669661645828 9.147179563467706E-4 68-69 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.382303962702616 9.147179563467706E-4 70-71 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.4194703975604953 9.628610066808111E-4 72-73 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.4578644802018927 0.0012035762583510138 74-75 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.5288032648691015 0.0013480054093531356 76-77 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.6155811130962096 0.0013480054093531356 78-79 3.8514440267232444E-4 1.9257220133616222E-4 0.0 0.7024311758988186 0.0013480054093531356 80-81 4.814305033404056E-4 1.9257220133616222E-4 0.0 0.7769325462907465 0.0013480054093531356 82-83 4.814305033404056E-4 1.9257220133616222E-4 0.0 0.8925962247182789 0.001372076934520156 84-85 4.814305033404056E-4 1.9257220133616222E-4 0.0 1.0901271602388474 0.0014442915100212166 86-87 4.814305033404056E-4 1.9257220133616222E-4 0.0 1.2765129796070853 0.0014924345603552572 88-89 4.814305033404056E-4 1.9257220133616222E-4 0.0 1.517420803478624 0.0015405776106892977 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 46.05254 1 GTATCAA 7730 0.0 35.70925 1 ATCAACG 9900 0.0 27.395576 3 TATCAAC 10005 0.0 27.299938 2 TCAACGC 10035 0.0 27.070448 4 CAACGCA 10365 0.0 26.300219 5 AACGCAG 10620 0.0 25.802252 6 TACCTGG 1870 0.0 23.623934 2 GTACATG 7345 0.0 23.415361 1 GTACCTG 1980 0.0 23.275051 1 TACATGG 7495 0.0 22.37247 2 ACGCAGA 12225 0.0 22.298176 7 CGCAGAG 12495 0.0 21.740328 8 ACATGGG 7425 0.0 21.494253 3 GAGTACT 7975 0.0 21.111193 12-13 GTAAGGT 845 0.0 20.795137 4 GCAGAGT 13395 0.0 20.315065 9 CATGGGG 4615 0.0 20.169779 4 GTACTTT 8355 0.0 19.753117 14-15 AGAGTAC 11645 0.0 19.412157 10-11 >>END_MODULE