FastQCFastQC Report
Thu 26 May 2016
SRR936435_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936435_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891100
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT145990.5049635086991111No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT95220.32935560859188545No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76660.265158590155996No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT69830.241534364082875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63490.2196049946387188No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56930.19691466915706823No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45720.1581405001556501No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40450.13991214416658018No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC38360.13268306181038358No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35050.1212341323371727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34550.11950468679741275No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31600.10930095811282903No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG29820.1031441319912836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122900.024.9302651
TACATGG124050.024.0864582
ACATGGG126750.022.8230343
GAGTACT83600.021.96303612-13
CTACGGT1404.8522642E-420.3583374
AGTACTT88550.019.7159612-13
GTACTTT94150.019.57900414-15
CATGGGA65500.018.928594
GTGTAGC18250.018.7408261
CATGGGG60200.018.4645394
AGAGTAC172500.017.68113310-11
ACTTTTT110550.017.08421916-17
CGACGAA9800.016.7275594-95
GTATAGC9700.016.1607421
TACTTTT109400.015.85092914-15
CCTATAC7100.015.3876933
GTATAGA8450.015.1784041
GTATCAA310050.014.7847721
AGTACAT95150.014.6277642
GTATAGG7550.014.4710471
Produced by FastQC (version 0.11.5)