FastQCFastQC Report
Thu 26 May 2016
SRR936435_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936435_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2891100
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT166810.5769776209747155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87950.3042094704437757No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT81010.2802047663519076No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78570.27176507211787904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT67770.23440904845906402No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC57720.19964719310988896No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT50640.17515824426688803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45930.15886686728234928No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43870.15174155165853828No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC42430.1467607485040296No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT40690.14074227802566497No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG34660.11988516481615993No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA33460.11573449552073606No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31110.10760610148386428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG122500.023.7726081
TACATGG124650.023.0568792
ACATGGG125300.021.760093
GAGTACT108650.021.31245412-13
ATACCGT2801.5807018E-920.3546186
AGTACTT113600.020.17472312-13
GTACTTT117400.019.7454314-15
ACCGTCG2302.0624975E-618.5846548
AGAGTAC195950.018.21633710-11
GTATAGA8200.017.959751
CATGGGG58350.017.8267864
CATGGGA66750.017.7892884
TACTTTT130550.017.55639514-15
ACTTTTT137400.017.19852416-17
TATACCG1952.2777672E-417.0503485
TACCGTC2807.150702E-716.9621817
TAGCCCT20350.015.4045614
AGTACAT95550.015.36261752
GTAAGCG2950.015.29524294-95
GTGTAGC17850.015.1701221
Produced by FastQC (version 0.11.5)