FastQCFastQC Report
Thu 26 May 2016
SRR936434_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936434_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2973496
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT146370.4922488545469709No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT96430.3242984016121091No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79040.2658150540643068No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT71680.24106304498139564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63950.2150667093549142No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC56580.19028106982487955No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT45740.1538256651429832No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39950.13435363625846478No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.12749302504526658No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC37750.12695493789129025No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC35200.1183791738747925No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC31020.1043216469771609No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG30130.10132853718316755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG127100.024.3754921
TACATGG128650.023.3796482
GAGTACT88750.022.67099812-13
ACATGGG131650.021.8723683
GTACTTT98850.020.257114-15
AGTACTT95100.020.0081812-13
GTGTAGC19200.019.551371
GCGCGAC1250.006023628619.0053029
CATGGGG61850.018.0545564
TAGCCCT19300.017.973154
AGAGTAC179750.017.94783410-11
GTATAGG7100.017.4006841
ACCGTAC1650.001461652517.2778388
ACTTTTT121100.017.08500116-17
CATGGGA70450.016.8623334
GGTATAC4901.8189894E-1216.4855791
TACTTTT115900.016.436814-15
GTGTAAG13950.016.3500231
GTGTAGG13800.016.1834141
GTAGCCC17000.015.9319883
Produced by FastQC (version 0.11.5)